JP2017517268A5 - - Google Patents
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- JP2017517268A5 JP2017517268A5 JP2016572578A JP2016572578A JP2017517268A5 JP 2017517268 A5 JP2017517268 A5 JP 2017517268A5 JP 2016572578 A JP2016572578 A JP 2016572578A JP 2016572578 A JP2016572578 A JP 2016572578A JP 2017517268 A5 JP2017517268 A5 JP 2017517268A5
- Authority
- JP
- Japan
- Prior art keywords
- controlled
- genetically modified
- gene
- genes
- silencing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108090000623 proteins and genes Proteins 0.000 claims description 41
- 230000002503 metabolic effect Effects 0.000 claims description 28
- 244000005700 microbiome Species 0.000 claims description 26
- 230000014509 gene expression Effects 0.000 claims description 24
- 230000030279 gene silencing Effects 0.000 claims description 22
- 102000004169 proteins and genes Human genes 0.000 claims description 18
- 230000004907 flux Effects 0.000 claims description 16
- 230000002779 inactivation Effects 0.000 claims description 15
- 235000015097 nutrients Nutrition 0.000 claims description 15
- 238000004519 manufacturing process Methods 0.000 claims description 13
- 230000002797 proteolythic effect Effects 0.000 claims description 13
- 108091005804 Peptidases Proteins 0.000 claims description 11
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 10
- 239000004365 Protease Substances 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 5
- 108091033409 CRISPR Proteins 0.000 claims description 4
- 238000010446 CRISPR interference Methods 0.000 claims description 4
- 230000015556 catabolic process Effects 0.000 claims description 4
- 238000006731 degradation reaction Methods 0.000 claims description 4
- 108020005004 Guide RNA Proteins 0.000 claims description 3
- 230000017854 proteolysis Effects 0.000 claims description 3
- 239000000411 inducer Substances 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 230000004151 fermentation Effects 0.000 claims 10
- 238000000855 fermentation Methods 0.000 claims 10
- 230000015572 biosynthetic process Effects 0.000 claims 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 8
- 241000588724 Escherichia coli Species 0.000 claims 7
- 101100174653 Dictyostelium discoideum g6pd-2 gene Proteins 0.000 claims 6
- 102000035195 Peptidases Human genes 0.000 claims 6
- 101150085516 ZWF1 gene Proteins 0.000 claims 6
- 235000018102 proteins Nutrition 0.000 claims 6
- 101150033131 sthA gene Proteins 0.000 claims 6
- 101150078419 zwf gene Proteins 0.000 claims 6
- 101150026856 zwf2 gene Proteins 0.000 claims 6
- 101100161530 Mus musculus Acsbg1 gene Proteins 0.000 claims 5
- 101150106096 gltA gene Proteins 0.000 claims 5
- 101150042350 gltA2 gene Proteins 0.000 claims 5
- 101150040445 lpd gene Proteins 0.000 claims 5
- 101150003321 lpdA gene Proteins 0.000 claims 5
- 101150007808 lpdC gene Proteins 0.000 claims 5
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 4
- 229910019142 PO4 Inorganic materials 0.000 claims 4
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims 4
- 230000002708 enhancing effect Effects 0.000 claims 4
- 239000011777 magnesium Substances 0.000 claims 4
- 229910052749 magnesium Inorganic materials 0.000 claims 4
- 229910052757 nitrogen Inorganic materials 0.000 claims 4
- 230000037361 pathway Effects 0.000 claims 4
- 239000010452 phosphate Substances 0.000 claims 4
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 4
- 229910052717 sulfur Inorganic materials 0.000 claims 4
- 239000011593 sulfur Substances 0.000 claims 4
- 230000005526 G1 to G0 transition Effects 0.000 claims 3
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 claims 3
- 235000004279 alanine Nutrition 0.000 claims 3
- 230000009368 gene silencing by RNA Effects 0.000 claims 3
- 230000037353 metabolic pathway Effects 0.000 claims 3
- 230000037426 transcriptional repression Effects 0.000 claims 3
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims 2
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 claims 2
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 claims 2
- OFOBLEOULBTSOW-UHFFFAOYSA-N malonic acid group Chemical group C(CC(=O)O)(=O)O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims 2
- 230000006337 proteolytic cleavage Effects 0.000 claims 2
- 230000001568 sexual effect Effects 0.000 claims 2
- 230000007704 transition Effects 0.000 claims 2
- KJTLQQUUPVSXIM-ZCFIWIBFSA-N (R)-mevalonic acid Chemical group OCC[C@](O)(C)CC(O)=O KJTLQQUUPVSXIM-ZCFIWIBFSA-N 0.000 claims 1
- -1 (i) the fabl Proteins 0.000 claims 1
- WTLNOANVTIKPEE-UHFFFAOYSA-N 2-acetyloxypropanoic acid Chemical compound OC(=O)C(C)OC(C)=O WTLNOANVTIKPEE-UHFFFAOYSA-N 0.000 claims 1
- KJTLQQUUPVSXIM-UHFFFAOYSA-N DL-mevalonic acid Natural products OCCC(O)(C)CC(O)=O KJTLQQUUPVSXIM-UHFFFAOYSA-N 0.000 claims 1
- LTYOQGRJFJAKNA-KKIMTKSISA-N Malonyl CoA Natural products S(C(=O)CC(=O)O)CCNC(=O)CCNC(=O)[C@@H](O)C(CO[P@](=O)(O[P@](=O)(OC[C@H]1[C@@H](OP(=O)(O)O)[C@@H](O)[C@@H](n2c3ncnc(N)c3nc2)O1)O)O)(C)C LTYOQGRJFJAKNA-KKIMTKSISA-N 0.000 claims 1
- 101100421548 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) sipB gene Proteins 0.000 claims 1
- OJFDKHTZOUZBOS-CITAKDKDSA-N acetoacetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 OJFDKHTZOUZBOS-CITAKDKDSA-N 0.000 claims 1
- 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 claims 1
- 230000003698 anagen phase Effects 0.000 claims 1
- 230000006698 induction Effects 0.000 claims 1
- 230000000977 initiatory effect Effects 0.000 claims 1
- LTYOQGRJFJAKNA-DVVLENMVSA-N malonyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 LTYOQGRJFJAKNA-DVVLENMVSA-N 0.000 claims 1
- 230000001404 mediated effect Effects 0.000 claims 1
- 239000002207 metabolite Substances 0.000 claims 1
- 230000001902 propagating effect Effects 0.000 claims 1
- 101150113992 sspB gene Proteins 0.000 claims 1
- 238000013518 transcription Methods 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000002103 transcriptional effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012226 gene silencing method Methods 0.000 description 1
- 229910052816 inorganic phosphate Inorganic materials 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 239000002924 silencing RNA Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020033434A JP6920491B2 (ja) | 2014-06-11 | 2020-02-28 | 合成代謝弁を用いた迅速かつ動的なフラックス制御のための組成物及び方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462010574P | 2014-06-11 | 2014-06-11 | |
| US62/010,574 | 2014-06-11 | ||
| PCT/US2015/035306 WO2016053397A2 (en) | 2014-06-11 | 2015-06-11 | Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020033434A Division JP6920491B2 (ja) | 2014-06-11 | 2020-02-28 | 合成代謝弁を用いた迅速かつ動的なフラックス制御のための組成物及び方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017517268A JP2017517268A (ja) | 2017-06-29 |
| JP2017517268A5 true JP2017517268A5 (enExample) | 2018-05-10 |
| JP6730199B2 JP6730199B2 (ja) | 2020-07-29 |
Family
ID=55631742
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016572578A Active JP6730199B2 (ja) | 2014-06-11 | 2015-06-11 | 合成代謝弁を用いた迅速かつ動的なフラックス制御のための組成物及び方法 |
| JP2020033434A Active JP6920491B2 (ja) | 2014-06-11 | 2020-02-28 | 合成代謝弁を用いた迅速かつ動的なフラックス制御のための組成物及び方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020033434A Active JP6920491B2 (ja) | 2014-06-11 | 2020-02-28 | 合成代謝弁を用いた迅速かつ動的なフラックス制御のための組成物及び方法 |
Country Status (12)
| Country | Link |
|---|---|
| US (4) | US10662426B2 (enExample) |
| EP (2) | EP4148127A1 (enExample) |
| JP (2) | JP6730199B2 (enExample) |
| AU (2) | AU2015324564B2 (enExample) |
| BR (1) | BR112016028741A2 (enExample) |
| DK (1) | DK3155102T5 (enExample) |
| ES (1) | ES2937642T3 (enExample) |
| GB (1) | GB2528177B (enExample) |
| HU (1) | HUE061007T2 (enExample) |
| IL (2) | IL249191B (enExample) |
| MY (2) | MY187588A (enExample) |
| WO (1) | WO2016053397A2 (enExample) |
Families Citing this family (47)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US20150166984A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting alpha-antitrypsin point mutations |
| HUE061007T2 (hu) * | 2014-06-11 | 2023-05-28 | Univ Duke | Gyors és dinamikus áramlásszabályozásra szolgáló összetételek és módszerek szintetikus matabolikus szelepek segítségével |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| SG10202104041PA (en) | 2015-10-23 | 2021-06-29 | Harvard College | Nucleobase editors and uses thereof |
| LT3604527T (lt) | 2016-06-02 | 2021-06-25 | Sigma-Aldrich Co., Llc | Programuojamų, dnr surišančių baltymų panaudojimas tiksliniam genomo modifikavimui pagerinti |
| WO2018020012A1 (en) * | 2016-07-29 | 2018-02-01 | Danmarks Tekniske Universitet | Methods for decoupling cell growth from production of biochemicals and recombinant polypeptides |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| SG11201903089RA (en) | 2016-10-14 | 2019-05-30 | Harvard College | Aav delivery of nucleobase editors |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| CN118256413A (zh) * | 2017-02-21 | 2024-06-28 | 杜克大学 | 用于稳健动态代谢控制的组合物和方法 |
| EP3592381A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Cancer vaccine |
| US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| WO2018165629A1 (en) | 2017-03-10 | 2018-09-13 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| WO2018176009A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| US11795443B2 (en) | 2017-10-16 | 2023-10-24 | The Broad Institute, Inc. | Uses of adenosine base editors |
| WO2019118949A1 (en) | 2017-12-15 | 2019-06-20 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| US12227776B2 (en) | 2018-06-13 | 2025-02-18 | Caribou Biosciences, Inc. | Engineered cascade components and cascade complexes |
| US10227576B1 (en) | 2018-06-13 | 2019-03-12 | Caribou Biosciences, Inc. | Engineered cascade components and cascade complexes |
| WO2019246488A1 (en) * | 2018-06-21 | 2019-12-26 | Duke University | Compositions and methods for the production of pyruvic acid and related products using dynamic metabolic control |
| EP3820495A4 (en) | 2018-07-09 | 2022-07-20 | The Broad Institute Inc. | RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES |
| JP7474245B2 (ja) * | 2018-09-19 | 2024-04-24 | コナゲン インコーポレイテッド | コリネバクテリウム宿主細胞における操作した分解タグ変異体による制御可能なタンパク質分解 |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| EP3942043A2 (en) | 2019-03-19 | 2022-01-26 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| WO2020214842A1 (en) | 2019-04-17 | 2020-10-22 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| US12577572B2 (en) | 2019-10-23 | 2026-03-17 | Duke University | Compositions, systems, and methods for high level expression of recombinant protein |
| KR20220154748A (ko) * | 2020-03-16 | 2022-11-22 | 듀크 유니버시티 | 개선된 i-e형 crispr에 기반한 유전자 침묵화를 위한 방법 및 조성물 |
| US20230183757A1 (en) * | 2020-04-03 | 2023-06-15 | Duke University | Methods and compositions for the production of xylitol from xylose utilizing dynamic metabolic control |
| BR112022019942A2 (pt) * | 2020-04-03 | 2022-12-13 | Univ Duke | Microrganismos e. coli geneticamente modificados para produzir xilitol a partir de xilose e bioprocessos de fermentação multiestágio para produzir xilitol |
| BR112022022603A2 (pt) | 2020-05-08 | 2023-01-17 | Broad Inst Inc | Métodos e composições para edição simultânea de ambas as fitas de sequência alvo de nucleotídeos de fita dupla |
| CR20230098A (es) * | 2020-07-24 | 2023-08-07 | Univ Duke | Métodos y composiciones para la producción de productos derivados del acetil-coa |
| WO2022226507A1 (en) * | 2021-04-20 | 2022-10-27 | Duke University | Two-stage dynamic control over redox state improves cystolic expression of disulfide containing proteins in e. coli |
| CN113604416B (zh) * | 2021-09-02 | 2023-05-05 | 天津大学 | 产乙偶姻的大肠杆菌工程菌及构建方法与在全细胞催化生产乙偶姻的应用 |
| WO2023183784A2 (en) * | 2022-03-21 | 2023-09-28 | Duke University | Compositions and methods for improved malonyl-coa biosynthesis using 2-stage dynamic metabolic control |
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| EP2562249A1 (en) * | 2011-08-24 | 2013-02-27 | Helmholtz-Zentrum für Infektionsforschung GmbH | Engineered host cells and methods for the production of cis, cis-muconic acid |
| LT3401400T (lt) * | 2012-05-25 | 2019-06-10 | The Regents Of The University Of California | Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui |
| CA2905049A1 (en) | 2013-03-13 | 2014-10-02 | Trustees Of Boston University | Tunable control of protein degradation in synthetic and endogenous bacterial systems |
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| EP3636743A1 (en) * | 2014-04-02 | 2020-04-15 | The Regents of The University of California | A synthetic biochemistry molecular purge valve module that maintains co-factor balance |
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| HUE061007T2 (hu) * | 2014-06-11 | 2023-05-28 | Univ Duke | Gyors és dinamikus áramlásszabályozásra szolgáló összetételek és módszerek szintetikus matabolikus szelepek segítségével |
| CN118256413A (zh) | 2017-02-21 | 2024-06-28 | 杜克大学 | 用于稳健动态代谢控制的组合物和方法 |
| WO2019246488A1 (en) * | 2018-06-21 | 2019-12-26 | Duke University | Compositions and methods for the production of pyruvic acid and related products using dynamic metabolic control |
-
2015
- 2015-06-11 HU HUE15845669A patent/HUE061007T2/hu unknown
- 2015-06-11 GB GB1511937.3A patent/GB2528177B/en active Active
- 2015-06-11 WO PCT/US2015/035306 patent/WO2016053397A2/en not_active Ceased
- 2015-06-11 US US15/317,768 patent/US10662426B2/en active Active
- 2015-06-11 MY MYPI2016001993A patent/MY187588A/en unknown
- 2015-06-11 DK DK15845669.9T patent/DK3155102T5/da active
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