LT3604527T - Programuojamų, dnr surišančių baltymų panaudojimas tiksliniam genomo modifikavimui pagerinti - Google Patents
Programuojamų, dnr surišančių baltymų panaudojimas tiksliniam genomo modifikavimui pagerintiInfo
- Publication number
- LT3604527T LT3604527T LTEP19186629.2T LT19186629T LT3604527T LT 3604527 T LT3604527 T LT 3604527T LT 19186629 T LT19186629 T LT 19186629T LT 3604527 T LT3604527 T LT 3604527T
- Authority
- LT
- Lithuania
- Prior art keywords
- binding proteins
- dna binding
- genome modification
- programmable dna
- targeted genome
- Prior art date
Links
- 102000052510 DNA-Binding Proteins Human genes 0.000 title 1
- 108700020911 DNA-Binding Proteins Proteins 0.000 title 1
- 230000004048 modification Effects 0.000 title 1
- 238000012986 modification Methods 0.000 title 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/06—Antianaemics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/30—Special therapeutic applications
- C12N2320/32—Special delivery means, e.g. tissue-specific
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01048—Histone acetyltransferase (2.3.1.48)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
- C12Y305/04001—Cytosine deaminase (3.5.4.1)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662344858P | 2016-06-02 | 2016-06-02 | |
US201662358415P | 2016-07-05 | 2016-07-05 |
Publications (1)
Publication Number | Publication Date |
---|---|
LT3604527T true LT3604527T (lt) | 2021-06-25 |
Family
ID=58108457
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
LTEP19186629.2T LT3604527T (lt) | 2016-06-02 | 2017-02-20 | Programuojamų, dnr surišančių baltymų panaudojimas tiksliniam genomo modifikavimui pagerinti |
Country Status (17)
Country | Link |
---|---|
US (3) | US10266851B2 (lt) |
EP (3) | EP3604527B1 (lt) |
JP (3) | JP6878468B2 (lt) |
KR (2) | KR20220147710A (lt) |
CN (2) | CN109983124B (lt) |
AU (4) | AU2017274145B2 (lt) |
BR (1) | BR112018074531B1 (lt) |
CA (2) | CA3026321C (lt) |
DK (2) | DK3272867T3 (lt) |
ES (2) | ES2881355T3 (lt) |
GB (3) | GB2552861B (lt) |
IL (5) | IL289989B (lt) |
LT (1) | LT3604527T (lt) |
PL (2) | PL3272867T3 (lt) |
PT (2) | PT3272867T (lt) |
SG (1) | SG11201810003UA (lt) |
WO (1) | WO2017209809A1 (lt) |
Families Citing this family (78)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
US9068179B1 (en) | 2013-12-12 | 2015-06-30 | President And Fellows Of Harvard College | Methods for correcting presenilin point mutations |
AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
IL258821B (en) | 2015-10-23 | 2022-07-01 | Harvard College | Nucleobase editors and their uses |
US11293021B1 (en) | 2016-06-23 | 2022-04-05 | Inscripta, Inc. | Automated cell processing methods, modules, instruments, and systems |
KR102547316B1 (ko) | 2016-08-03 | 2023-06-23 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
US11078481B1 (en) | 2016-08-03 | 2021-08-03 | KSQ Therapeutics, Inc. | Methods for screening for cancer targets |
CN109804066A (zh) | 2016-08-09 | 2019-05-24 | 哈佛大学的校长及成员们 | 可编程cas9-重组酶融合蛋白及其用途 |
WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
US11078483B1 (en) | 2016-09-02 | 2021-08-03 | KSQ Therapeutics, Inc. | Methods for measuring and improving CRISPR reagent function |
KR102622411B1 (ko) | 2016-10-14 | 2024-01-10 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
GB2575930A (en) | 2017-03-23 | 2020-01-29 | Harvard College | Nucleobase editors comprising nucleic acid programmable DNA binding proteins |
US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
US10011849B1 (en) | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
SI3645719T1 (sl) | 2017-06-30 | 2022-07-29 | Inscripta, Inc., | Postopki, moduli, instrumenti in sistemi za avtomatizirano obdelavo celic |
JP2020534795A (ja) | 2017-07-28 | 2020-12-03 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ファージによって支援される連続的進化(pace)を用いて塩基編集因子を進化させるための方法および組成物 |
BR112019028146A2 (pt) * | 2017-07-31 | 2020-07-07 | Sigma-Aldrich Co. Llc | rna guia sintético para sistemas ativadores de crispr/cas |
EP3676376A2 (en) | 2017-08-30 | 2020-07-08 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
US11709156B2 (en) | 2017-09-18 | 2023-07-25 | Waters Technologies Corporation | Use of vapor deposition coated flow paths for improved analytical analysis |
US11709155B2 (en) | 2017-09-18 | 2023-07-25 | Waters Technologies Corporation | Use of vapor deposition coated flow paths for improved chromatography of metal interacting analytes |
WO2019079347A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | USES OF BASIC EDITORS ADENOSINE |
WO2019126709A1 (en) * | 2017-12-22 | 2019-06-27 | The Broad Institute, Inc. | Cas12b systems, methods, and compositions for targeted dna base editing |
EP3728576A4 (en) * | 2017-12-22 | 2021-11-24 | The Broad Institute, Inc. | CAS12B SYSTEMS, METHODS AND COMPOSITIONS FOR SPECIFIC EDITING OF RNA BASES |
CA3084020A1 (en) * | 2018-02-15 | 2019-08-22 | Sigma-Aldrich Co. Llc | Engineered cas9 systems for eukaryotic genome modification |
WO2019200004A1 (en) | 2018-04-13 | 2019-10-17 | Inscripta, Inc. | Automated cell processing instruments comprising reagent cartridges |
US10526598B2 (en) | 2018-04-24 | 2020-01-07 | Inscripta, Inc. | Methods for identifying T-cell receptor antigens |
US10501738B2 (en) | 2018-04-24 | 2019-12-10 | Inscripta, Inc. | Automated instrumentation for production of peptide libraries |
US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
EP3830301A1 (en) | 2018-08-01 | 2021-06-09 | Mammoth Biosciences, Inc. | Programmable nuclease compositions and methods of use thereof |
US10532324B1 (en) | 2018-08-14 | 2020-01-14 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
US10533152B1 (en) | 2018-08-14 | 2020-01-14 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
WO2020041313A1 (en) * | 2018-08-21 | 2020-02-27 | Sigma-Aldrich Co. Llc | Down-regulation of the cytosolic dna sensor pathway |
CN112867792A (zh) * | 2018-08-23 | 2021-05-28 | 桑格摩生物治疗股份有限公司 | 工程化靶特异性碱基编辑器 |
EP3870697A4 (en) | 2018-10-22 | 2022-11-09 | Inscripta, Inc. | MODIFIED ENZYMES |
US11214781B2 (en) | 2018-10-22 | 2022-01-04 | Inscripta, Inc. | Engineered enzyme |
EP3931313A2 (en) | 2019-01-04 | 2022-01-05 | Mammoth Biosciences, Inc. | Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection |
CN110804628B (zh) * | 2019-02-28 | 2023-05-12 | 中国科学院脑科学与智能技术卓越创新中心 | 高特异性无脱靶单碱基基因编辑工具 |
WO2020191249A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
US11001831B2 (en) | 2019-03-25 | 2021-05-11 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
AU2020247900A1 (en) | 2019-03-25 | 2021-11-04 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
WO2020247587A1 (en) | 2019-06-06 | 2020-12-10 | Inscripta, Inc. | Curing for recursive nucleic acid-guided cell editing |
EP3986909A4 (en) | 2019-06-21 | 2023-08-02 | Inscripta, Inc. | GENOME-WIDE RATIONAL DESIGNED MUTATIONS LEADING TO INCREASED LYSINE PRODUCTION IN E. COLI |
US10927385B2 (en) | 2019-06-25 | 2021-02-23 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
EP3783104A1 (en) * | 2019-08-20 | 2021-02-24 | Kemijski Institut | Coiled-coil mediated tethering of crispr-cas and exonucleases for enhanced genome editing |
WO2021102059A1 (en) | 2019-11-19 | 2021-05-27 | Inscripta, Inc. | Methods for increasing observed editing in bacteria |
WO2021118626A1 (en) | 2019-12-10 | 2021-06-17 | Inscripta, Inc. | Novel mad nucleases |
US10704033B1 (en) | 2019-12-13 | 2020-07-07 | Inscripta, Inc. | Nucleic acid-guided nucleases |
CA3161802A1 (en) * | 2019-12-17 | 2021-06-24 | Aridis Pharmaceuticals, Inc. | Methods of enhancing and expediting expression of antibodies |
JP2023507566A (ja) | 2019-12-18 | 2023-02-24 | インスクリプタ, インコーポレイテッド | 核酸誘導ヌクレアーゼ編集済み細胞のin vivo検出のためのカスケード/dCas3相補性アッセイ |
US20230037794A1 (en) * | 2019-12-23 | 2023-02-09 | The Broad Institute, Inc. | Programmable dna nuclease-associated ligase and methods of use thereof |
US10689669B1 (en) | 2020-01-11 | 2020-06-23 | Inscripta, Inc. | Automated multi-module cell processing methods, instruments, and systems |
US11918936B2 (en) | 2020-01-17 | 2024-03-05 | Waters Technologies Corporation | Performance and dynamic range for oligonucleotide bioanalysis through reduction of non specific binding |
CN115135370A (zh) | 2020-01-27 | 2022-09-30 | 因思科瑞普特公司 | 电穿孔模块和仪器 |
US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
MX2022014008A (es) | 2020-05-08 | 2023-02-09 | Broad Inst Inc | Métodos y composiciones para la edición simultánea de ambas cadenas de una secuencia de nucleótidos de doble cadena objetivo. |
US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
WO2022060749A1 (en) | 2020-09-15 | 2022-03-24 | Inscripta, Inc. | Crispr editing to embed nucleic acid landing pads into genomes of live cells |
JP2023541990A (ja) * | 2020-09-18 | 2023-10-04 | インスティチュート フォー ベーシック サイエンス | 標的化された脱アミノ酵素およびそれを用いた塩基編集 |
US11512297B2 (en) | 2020-11-09 | 2022-11-29 | Inscripta, Inc. | Affinity tag for recombination protein recruitment |
AU2021415461A1 (en) | 2021-01-04 | 2023-08-17 | Inscripta, Inc. | Mad nucleases |
WO2022150269A1 (en) | 2021-01-07 | 2022-07-14 | Inscripta, Inc. | Mad nucleases |
WO2022163770A1 (ja) * | 2021-01-28 | 2022-08-04 | 国立研究開発法人理化学研究所 | ゲノム編集ツールの評価方法 |
US11884924B2 (en) | 2021-02-16 | 2024-01-30 | Inscripta, Inc. | Dual strand nucleic acid-guided nickase editing |
CN115261359B (zh) * | 2021-05-21 | 2023-06-30 | 山东舜丰生物科技有限公司 | 一种新型crispr酶和系统以及应用 |
CN114250172B (zh) * | 2021-12-09 | 2023-05-23 | 中国科学院海洋研究所 | 一种海运海杆菌及其应用 |
CN114507689B (zh) * | 2022-01-27 | 2024-01-16 | 山东大学 | 一种提高真核生物基因编辑效率的方法及其应用 |
CN114606251B (zh) * | 2022-03-30 | 2023-02-28 | 呈诺再生医学科技(珠海横琴新区)有限公司 | 以外泌体为载体的dna递送系统 |
WO2024063538A1 (ko) * | 2022-09-21 | 2024-03-28 | 기초과학연구원 | 식물세포 소기관 dna의 염기 교정 |
Family Cites Families (63)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS501B1 (lt) | 1970-05-19 | 1975-01-06 | ||
JP2610720B2 (ja) | 1991-06-24 | 1997-05-14 | 株式会社クボタ | 作業機のローリング制御装置 |
US6534261B1 (en) * | 1999-01-12 | 2003-03-18 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
GB9915126D0 (en) * | 1999-06-30 | 1999-09-01 | Imp College Innovations Ltd | Control of gene expression |
WO2001083751A2 (en) | 2000-04-28 | 2001-11-08 | Sangamo Biosciences, Inc. | Methods for binding an exogenous molecule to cellular chromatin |
AU2002360424A1 (en) | 2001-11-26 | 2003-06-10 | Advanced Cell Technology, Inc. | Methods for making and using reprogrammed human somatic cell nuclei and autologous and isogenic human stem cells |
WO2005014791A2 (en) * | 2003-08-08 | 2005-02-17 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
US7972854B2 (en) | 2004-02-05 | 2011-07-05 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
US20050220796A1 (en) | 2004-03-31 | 2005-10-06 | Dynan William S | Compositions and methods for modulating DNA repair |
EP1913149A4 (en) | 2005-07-26 | 2009-08-05 | Sangamo Biosciences Inc | TARGETED INTEGRATION AND EXPRESSION OF EXOGENOUS NUCLEIC ACID SEQUENCES |
WO2007102618A1 (en) * | 2006-03-08 | 2007-09-13 | Kyoto University | Nucleic acid cleaving agent |
EP2054514A4 (en) | 2006-08-04 | 2009-11-04 | Univ Georgia State Res Found | ENZYME SENSORS, METHODS OF PREPARATION AND USE OF SUCH SENSORS, AND METHODS OF DETECTING PROTEASE ACTIVITY |
PT2126130E (pt) | 2007-03-02 | 2015-08-03 | Dupont Nutrition Biosci Aps | Culturas com resistência melhorada a fagos |
GB0806086D0 (en) | 2008-04-04 | 2008-05-14 | Ulive Entpr Ltd | Dendrimer polymer hybrids |
US8546553B2 (en) | 2008-07-25 | 2013-10-01 | University Of Georgia Research Foundation, Inc. | Prokaryotic RNAi-like system and methods of use |
US20100076057A1 (en) | 2008-09-23 | 2010-03-25 | Northwestern University | TARGET DNA INTERFERENCE WITH crRNA |
WO2010054108A2 (en) | 2008-11-06 | 2010-05-14 | University Of Georgia Research Foundation, Inc. | Cas6 polypeptides and methods of use |
WO2010075424A2 (en) | 2008-12-22 | 2010-07-01 | The Regents Of University Of California | Compositions and methods for downregulating prokaryotic genes |
US20120192298A1 (en) | 2009-07-24 | 2012-07-26 | Sigma Aldrich Co. Llc | Method for genome editing |
US8586526B2 (en) | 2010-05-17 | 2013-11-19 | Sangamo Biosciences, Inc. | DNA-binding proteins and uses thereof |
JP2013518602A (ja) | 2010-02-09 | 2013-05-23 | サンガモ バイオサイエンシーズ, インコーポレイテッド | 部分的に一本鎖のドナー分子による標的化ゲノム改変 |
US10087431B2 (en) | 2010-03-10 | 2018-10-02 | The Regents Of The University Of California | Methods of generating nucleic acid fragments |
EA024121B9 (ru) | 2010-05-10 | 2017-01-30 | Дзе Реджентс Ов Дзе Юниверсити Ов Калифорния | Композиции эндорибонуклеаз и способы их использования |
JP2013537410A (ja) | 2010-07-23 | 2013-10-03 | シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニー | 標的化エンドヌクレアーゼおよび一本鎖核酸を用いたゲノム編集 |
US20140113376A1 (en) | 2011-06-01 | 2014-04-24 | Rotem Sorek | Compositions and methods for downregulating prokaryotic genes |
US9458205B2 (en) | 2011-11-16 | 2016-10-04 | Sangamo Biosciences, Inc. | Modified DNA-binding proteins and uses thereof |
GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
WO2013141680A1 (en) | 2012-03-20 | 2013-09-26 | Vilnius University | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
EP2847338B1 (en) | 2012-05-07 | 2018-09-19 | Sangamo Therapeutics, Inc. | Methods and compositions for nuclease-mediated targeted integration of transgenes |
PL2800811T3 (pl) | 2012-05-25 | 2017-11-30 | Emmanuelle Charpentier | Sposoby i kompozycje do kierowanej RNA modyfikacji docelowego DNA i do modulacji transkrypcji kierowanej RNA |
PT2912175T (pt) | 2012-10-23 | 2018-11-05 | Toolgen Inc | Composição para a clivagem de um adn alvo que compreende um arn guia específico para o adn alvo e ácido nucleico que codifica a proteína cas ou a proteína cas e sua utilização |
PT3363902T (pt) | 2012-12-06 | 2019-12-19 | Sigma Aldrich Co Llc | Modificação e regulação de genoma baseadas em crispr |
US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
US8993233B2 (en) | 2012-12-12 | 2015-03-31 | The Broad Institute Inc. | Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains |
MX2015007743A (es) | 2012-12-17 | 2015-12-07 | Harvard College | Ingenieria del genoma humano guiada por ácido robonucleico. |
CN103233028B (zh) | 2013-01-25 | 2015-05-13 | 南京徇齐生物技术有限公司 | 一种无物种限制无生物安全性问题的真核生物基因打靶方法及螺旋结构dna序列 |
US20140315985A1 (en) | 2013-03-14 | 2014-10-23 | Caribou Biosciences, Inc. | Compositions and methods of nucleic acid-targeting nucleic acids |
US20140273230A1 (en) | 2013-03-15 | 2014-09-18 | Sigma-Aldrich Co., Llc | Crispr-based genome modification and regulation |
CN112301024A (zh) * | 2013-03-15 | 2021-02-02 | 通用医疗公司 | 使用RNA引导的FokI核酸酶(RFN)提高RNA引导的基因组编辑的特异性 |
WO2014191518A1 (en) * | 2013-05-29 | 2014-12-04 | Cellectis | A method for producing precise dna cleavage using cas9 nickase activity |
EP3603679B1 (en) * | 2013-06-04 | 2022-08-10 | President and Fellows of Harvard College | Rna-guided transcriptional regulation |
WO2014197748A2 (en) * | 2013-06-05 | 2014-12-11 | Duke University | Rna-guided gene editing and gene regulation |
CN103343120B (zh) | 2013-07-04 | 2015-03-04 | 中国科学院遗传与发育生物学研究所 | 一种小麦基因组定点改造方法 |
CN103388006B (zh) | 2013-07-26 | 2015-10-28 | 华东师范大学 | 一种基因定点突变的构建方法 |
WO2015021426A1 (en) * | 2013-08-09 | 2015-02-12 | Sage Labs, Inc. | A crispr/cas system-based novel fusion protein and its application in genome editing |
US9322037B2 (en) * | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
JP6606088B2 (ja) * | 2014-02-24 | 2019-11-13 | サンガモ セラピューティクス, インコーポレイテッド | ヌクレアーゼ媒介性標的化組み込みのための方法および組成物 |
EP3167071B1 (en) * | 2014-07-09 | 2020-10-07 | Gen9, Inc. | Compositions and methods for site-directed dna nicking and cleaving |
AU2015298571B2 (en) * | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
EP3686279B1 (en) | 2014-08-17 | 2023-01-04 | The Broad Institute, Inc. | Genome editing using cas9 nickases |
DK3186376T3 (da) * | 2014-08-27 | 2019-05-06 | Caribou Biosciences Inc | Fremgangsmåder til forbedring af cas9-medieret manipulationseffektivitet |
US10570418B2 (en) * | 2014-09-02 | 2020-02-25 | The Regents Of The University Of California | Methods and compositions for RNA-directed target DNA modification |
WO2016065364A1 (en) * | 2014-10-24 | 2016-04-28 | Life Technologies Corporation | Compositions and methods for enhancing homologous recombination |
WO2016073990A2 (en) | 2014-11-07 | 2016-05-12 | Editas Medicine, Inc. | Methods for improving crispr/cas-mediated genome-editing |
US10190106B2 (en) * | 2014-12-22 | 2019-01-29 | Univesity Of Massachusetts | Cas9-DNA targeting unit chimeras |
GB201506509D0 (en) * | 2015-04-16 | 2015-06-03 | Univ Wageningen | Nuclease-mediated genome editing |
US9790490B2 (en) * | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
EP3344771A4 (en) * | 2015-08-31 | 2019-03-20 | Agilent Technologies, Inc. | COMPOUNDS AND METHODS FOR GENOME EDITING BASED ON CRISPR / CAS BY HOMOLOGOUS RECOMBINATION |
WO2017070598A1 (en) * | 2015-10-23 | 2017-04-27 | Caribou Biosciences, Inc. | Engineered crispr class 2 cross-type nucleic-acid targeting nucleic acids |
NZ742040A (en) * | 2015-12-04 | 2019-08-30 | Caribou Biosciences Inc | Engineered nucleic-acid targeting nucleic acids |
CN109152808A (zh) * | 2016-04-29 | 2019-01-04 | 生物辐射实验室股份有限公司 | 用于核酸序列的特异性靶向的二聚蛋白质 |
EP3872177A1 (en) * | 2016-05-27 | 2021-09-01 | Life Technologies Corporation | Compositions and methods for enhancing homologous recombination |
-
2017
- 2017-02-20 ES ES19186629T patent/ES2881355T3/es active Active
- 2017-02-20 CA CA3026321A patent/CA3026321C/en active Active
- 2017-02-20 ES ES17157024T patent/ES2760477T3/es active Active
- 2017-02-20 EP EP19186629.2A patent/EP3604527B1/en active Active
- 2017-02-20 CA CA3209273A patent/CA3209273A1/en active Pending
- 2017-02-20 JP JP2018562948A patent/JP6878468B2/ja active Active
- 2017-02-20 LT LTEP19186629.2T patent/LT3604527T/lt unknown
- 2017-02-20 CN CN201780048469.4A patent/CN109983124B/zh active Active
- 2017-02-20 DK DK17157024T patent/DK3272867T3/da active
- 2017-02-20 US US15/437,148 patent/US10266851B2/en active Active
- 2017-02-20 DK DK19186629.2T patent/DK3604527T3/da active
- 2017-02-20 EP EP17157024.5A patent/EP3272867B1/en active Active
- 2017-02-20 GB GB1702743.4A patent/GB2552861B/en active Active
- 2017-02-20 KR KR1020227036504A patent/KR20220147710A/ko active Application Filing
- 2017-02-20 GB GB2010846.0A patent/GB2582731B8/en not_active Expired - Fee Related
- 2017-02-20 EP EP21160880.7A patent/EP3907286A1/en active Pending
- 2017-02-20 BR BR112018074531-6A patent/BR112018074531B1/pt active IP Right Grant
- 2017-02-20 PL PL17157024T patent/PL3272867T3/pl unknown
- 2017-02-20 IL IL289989A patent/IL289989B/en unknown
- 2017-02-20 GB GB1904166.4A patent/GB2578802B8/en active Active
- 2017-02-20 PT PT171570245T patent/PT3272867T/pt unknown
- 2017-02-20 PT PT191866292T patent/PT3604527T/pt unknown
- 2017-02-20 CN CN202211060110.8A patent/CN116064529A/zh active Pending
- 2017-02-20 IL IL302238A patent/IL302238A/en unknown
- 2017-02-20 AU AU2017274145A patent/AU2017274145B2/en active Active
- 2017-02-20 SG SG11201810003UA patent/SG11201810003UA/en unknown
- 2017-02-20 KR KR1020187037407A patent/KR102458395B1/ko active IP Right Grant
- 2017-02-20 PL PL19186629T patent/PL3604527T3/pl unknown
- 2017-02-20 WO PCT/US2017/018589 patent/WO2017209809A1/en active Application Filing
- 2017-02-20 IL IL295358A patent/IL295358B2/en unknown
-
2018
- 2018-11-07 IL IL262854A patent/IL262854B/en active IP Right Grant
-
2019
- 2019-02-15 US US16/277,638 patent/US20190169651A1/en active Pending
- 2019-02-15 US US16/277,614 patent/US20190169650A1/en active Pending
-
2020
- 2020-06-09 IL IL275244A patent/IL275244B/en unknown
- 2020-09-30 AU AU2020244497A patent/AU2020244497B2/en active Active
-
2021
- 2021-02-01 AU AU2021200636A patent/AU2021200636B2/en active Active
- 2021-04-28 JP JP2021076124A patent/JP7220737B2/ja active Active
-
2023
- 2023-01-31 JP JP2023012725A patent/JP2023065365A/ja active Pending
- 2023-05-31 AU AU2023203391A patent/AU2023203391A1/en active Pending
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
IL295358A (en) | Use of programmable DNA-binding proteins to enhance targeted genome modification | |
SG10201913505WA (en) | Truncated crispr-cas proteins for dna targeting | |
HK1249527A1 (zh) | Cd137特異性的蛋白 | |
PL3708668T3 (pl) | Sztuczne cząsteczki kwasu nukleinowego dla poprawy ekspresji białka | |
IL274926A (en) | Pyrazolopyrimidines with activity against respiratory syncytial virus | |
HK1245293A1 (zh) | 新的對綠膿菌熒光素和綠膿菌螯鐵蛋白特異性的蛋白質 | |
HK1223956A1 (zh) | 利用 基序的 結合性蛋白質及其應用 | |
IL250796B (en) | lox1-specific binding proteins and their uses | |
IL256298A (en) | Multi-specific binding proteins | |
IL265942A (en) | A nucleotide sequence expressing an exosome anchor protein for use as a component | |
GB201517241D0 (en) | DNA modification | |
EP3265589A4 (en) | Polynucleotide binding protein sequencing cross reference to related applications | |
ZA201901862B (en) | Modified factor h binding protein | |
EP3259355A4 (en) | Methods related to dna sequencing | |
SG11201702843XA (en) | Light-activated two-component protein binding matrix |