JP2017513477A5 - - Google Patents
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- JP2017513477A5 JP2017513477A5 JP2016562503A JP2016562503A JP2017513477A5 JP 2017513477 A5 JP2017513477 A5 JP 2017513477A5 JP 2016562503 A JP2016562503 A JP 2016562503A JP 2016562503 A JP2016562503 A JP 2016562503A JP 2017513477 A5 JP2017513477 A5 JP 2017513477A5
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- JP
- Japan
- Prior art keywords
- cell
- cells
- dna
- nucleic acids
- isolated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims description 101
- 210000004027 cell Anatomy 0.000 claims description 80
- 108020004414 DNA Proteins 0.000 claims description 55
- 230000004048 modification Effects 0.000 claims description 33
- 238000012986 modification Methods 0.000 claims description 33
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 22
- 150000007523 nucleic acids Chemical class 0.000 claims description 20
- 238000004520 electroporation Methods 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 18
- 108020004707 nucleic acids Proteins 0.000 claims description 18
- 102000039446 nucleic acids Human genes 0.000 claims description 18
- 210000000130 stem cell Anatomy 0.000 claims description 9
- 101710163270 Nuclease Proteins 0.000 claims description 8
- 230000003833 cell viability Effects 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 210000004962 mammalian cell Anatomy 0.000 claims description 6
- 229940124568 digestive agent Drugs 0.000 claims description 5
- 210000003958 hematopoietic stem cell Anatomy 0.000 claims description 5
- 108020004705 Codon Proteins 0.000 claims description 4
- 108091034117 Oligonucleotide Proteins 0.000 claims description 4
- 238000012216 screening Methods 0.000 claims description 4
- 238000001890 transfection Methods 0.000 claims description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 3
- 230000001902 propagating effect Effects 0.000 claims description 3
- 102000040650 (ribonucleotides)n+m Human genes 0.000 claims description 2
- 108091033409 CRISPR Proteins 0.000 claims description 2
- 108020005004 Guide RNA Proteins 0.000 claims description 2
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 claims description 2
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 claims description 2
- 102100034343 Integrase Human genes 0.000 claims description 2
- 108010061833 Integrases Proteins 0.000 claims description 2
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 2
- 238000010459 TALEN Methods 0.000 claims description 2
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 claims description 2
- 102000008579 Transposases Human genes 0.000 claims description 2
- 108010020764 Transposases Proteins 0.000 claims description 2
- 230000004075 alteration Effects 0.000 claims description 2
- 210000001185 bone marrow Anatomy 0.000 claims description 2
- 239000002458 cell surface marker Substances 0.000 claims description 2
- 238000010790 dilution Methods 0.000 claims description 2
- 239000012895 dilution Substances 0.000 claims description 2
- 210000002950 fibroblast Anatomy 0.000 claims description 2
- 230000008014 freezing Effects 0.000 claims description 2
- 238000007710 freezing Methods 0.000 claims description 2
- 210000005260 human cell Anatomy 0.000 claims description 2
- 238000011031 large-scale manufacturing process Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims description 2
- 239000002773 nucleotide Substances 0.000 claims description 2
- 125000003729 nucleotide group Chemical group 0.000 claims description 2
- 210000005105 peripheral blood lymphocyte Anatomy 0.000 claims description 2
- 239000012679 serum free medium Substances 0.000 claims description 2
- 230000003612 virological effect Effects 0.000 claims description 2
- 102000053602 DNA Human genes 0.000 claims 1
- 108020004682 Single-Stranded DNA Proteins 0.000 claims 1
- 230000008685 targeting Effects 0.000 claims 1
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 101150013707 HBB gene Proteins 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102100025621 Cytochrome b-245 heavy chain Human genes 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 208000016532 chronic granulomatous disease Diseases 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461979178P | 2014-04-14 | 2014-04-14 | |
| US61/979,178 | 2014-04-14 | ||
| US201462078706P | 2014-11-12 | 2014-11-12 | |
| US62/078,706 | 2014-11-12 | ||
| PCT/US2015/025523 WO2015160683A1 (en) | 2014-04-14 | 2015-04-13 | Methods and compositions for modifying genomic dna |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020127917A Division JP7209671B2 (ja) | 2014-04-14 | 2020-07-29 | ゲノムｄｎａを改変するための方法および組成物 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2017513477A JP2017513477A (ja) | 2017-06-01 |
| JP2017513477A5 true JP2017513477A5 (OSRAM) | 2018-05-24 |
Family
ID=54324457
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016562503A Pending JP2017513477A (ja) | 2014-04-14 | 2015-04-13 | ゲノムｄｎａを改変するための方法および組成物 |
| JP2020127917A Active JP7209671B2 (ja) | 2014-04-14 | 2020-07-29 | ゲノムｄｎａを改変するための方法および組成物 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020127917A Active JP7209671B2 (ja) | 2014-04-14 | 2020-07-29 | ゲノムｄｎａを改変するための方法および組成物 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20170029805A1 (OSRAM) |
| EP (1) | EP3132025B1 (OSRAM) |
| JP (2) | JP2017513477A (OSRAM) |
| KR (1) | KR102406585B1 (OSRAM) |
| CN (1) | CN106687585B (OSRAM) |
| ES (1) | ES2962509T3 (OSRAM) |
| SG (1) | SG11201608503UA (OSRAM) |
| WO (1) | WO2015160683A1 (OSRAM) |
Families Citing this family (78)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6261500B2 (ja) | 2011-07-22 | 2018-01-17 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ヌクレアーゼ切断特異性の評価および改善 |
| JP6683598B2 (ja) | 2013-03-15 | 2020-04-22 | マックスサイト インコーポレーティッド | 安定なトランスフェクトされた細胞を生成するための方法および組成物 |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| WO2015066119A1 (en) | 2013-10-30 | 2015-05-07 | North Carolina State University | Compositions and methods related to a type-ii crispr-cas system in lactobacillus buchneri |
| PT3066201T (pt) | 2013-11-07 | 2018-06-04 | Massachusetts Inst Technology | Métodos e composições relacionadas com crispr com arng reguladores |
| US9068179B1 (en) | 2013-12-12 | 2015-06-30 | President And Fellows Of Harvard College | Methods for correcting presenilin point mutations |
| US11439712B2 (en) | 2014-04-08 | 2022-09-13 | North Carolina State University | Methods and compositions for RNA-directed repression of transcription using CRISPR-associated genes |
| GB201407852D0 (en) | 2014-05-02 | 2014-06-18 | Iontas Ltd | Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| WO2016033298A1 (en) | 2014-08-28 | 2016-03-03 | North Carolina State University | Novel cas9 proteins and guiding features for dna targeting and genome editing |
| AU2015330699B2 (en) | 2014-10-10 | 2021-12-02 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| AU2015342749B2 (en) | 2014-11-07 | 2022-01-27 | Editas Medicine, Inc. | Methods for improving CRISPR/Cas-mediated genome-editing |
| ES2926467T3 (es) | 2015-04-13 | 2022-10-26 | Maxcyte Inc | Métodos y composiciones para la modificación del ADN genómico |
| CN108026536A (zh) | 2015-05-29 | 2018-05-11 | 北卡罗来纳州立大学 | 使用crispr核酸筛选细菌、古细菌、藻类和酵母的方法 |
| EP4299754A3 (en) | 2015-06-15 | 2024-03-20 | North Carolina State University | Methods and compositions for efficient delivery of nucleic acids and rna-based antimicrobials |
| WO2017053879A1 (en) | 2015-09-24 | 2017-03-30 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
| US11286480B2 (en) | 2015-09-28 | 2022-03-29 | North Carolina State University | Methods and compositions for sequence specific antimicrobials |
| EP3365356B1 (en) | 2015-10-23 | 2023-06-28 | President and Fellows of Harvard College | Nucleobase editors and uses thereof |
| US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
| US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
| WO2017112620A1 (en) | 2015-12-22 | 2017-06-29 | North Carolina State University | Methods and compositions for delivery of crispr based antimicrobials |
| EP3433363A1 (en) | 2016-03-25 | 2019-01-30 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| US11236313B2 (en) | 2016-04-13 | 2022-02-01 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
| KR20190031306A (ko) * | 2016-07-21 | 2019-03-25 | 맥스시티 인코포레이티드 | 게놈 dna를 변경하기 위한 방법 및 조성물 |
| EP4613289A2 (en) * | 2016-07-27 | 2025-09-10 | The Board of Trustees of the Leland Stanford Junior University | Immolative cell-penetrating complexes for nucleic acid delivery |
| JP7231935B2 (ja) | 2016-08-03 | 2023-03-08 | プレジデント アンド フェローズ オブ ハーバード カレッジ | アデノシン核酸塩基編集因子およびそれらの使用 |
| EP3497214B1 (en) | 2016-08-09 | 2023-06-28 | President and Fellows of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| EP3526320A1 (en) | 2016-10-14 | 2019-08-21 | President and Fellows of Harvard College | Aav delivery of nucleobase editors |
| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| WO2018165631A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Cancer vaccine |
| WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| KR20190127797A (ko) | 2017-03-10 | 2019-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 시토신에서 구아닌으로의 염기 편집제 |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| WO2018232382A1 (en) * | 2017-06-16 | 2018-12-20 | Applied Stemcell, Inc. | Gene editing methods with increased knock-in efficiency |
| EP3848459A1 (en) | 2017-06-30 | 2021-07-14 | Inscripta, Inc. | Automated cell processing methods, modules, instruments and systems |
| US11866726B2 (en) | 2017-07-14 | 2024-01-09 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
| WO2019023680A1 (en) | 2017-07-28 | 2019-01-31 | President And Fellows Of Harvard College | METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE) |
| US10738327B2 (en) | 2017-08-28 | 2020-08-11 | Inscripta, Inc. | Electroporation cuvettes for automation |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| JP6985508B2 (ja) | 2017-09-30 | 2021-12-22 | インスクリプタ， インコーポレイテッド | フロースルーエレクトロポレーション機器編成 |
| CN111757937A (zh) | 2017-10-16 | 2020-10-09 | 布罗德研究所股份有限公司 | 腺苷碱基编辑器的用途 |
| WO2019118949A1 (en) | 2017-12-15 | 2019-06-20 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| BR112020014989A2 (pt) | 2018-02-01 | 2020-12-29 | Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences | Método aprimorado para edição de genoma |
| EP3775159A4 (en) | 2018-03-29 | 2022-01-19 | Inscripta, Inc. | AUTOMATED REGULATION OF CELL GROWTH RATES FOR INDUCTION AND TRANSFORMATION |
| WO2019200004A1 (en) | 2018-04-13 | 2019-10-17 | Inscripta, Inc. | Automated cell processing instruments comprising reagent cartridges |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| US10557216B2 (en) | 2018-04-24 | 2020-02-11 | Inscripta, Inc. | Automated instrumentation for production of T-cell receptor peptide libraries |
| US10501738B2 (en) | 2018-04-24 | 2019-12-10 | Inscripta, Inc. | Automated instrumentation for production of peptide libraries |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| WO2020005383A1 (en) | 2018-06-30 | 2020-01-02 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| US10532324B1 (en) | 2018-08-14 | 2020-01-14 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US10752874B2 (en) | 2018-08-14 | 2020-08-25 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| CA3108892A1 (en) | 2018-08-30 | 2020-04-23 | Inscripta, Inc. | Improved detection of nuclease edited sequences in automated modules and instruments |
| WO2020072253A1 (en) | 2018-10-01 | 2020-04-09 | North Carolina State University | Recombinant type i crispr-cas system and uses thereof for screening for variant cells |
| US12264313B2 (en) | 2018-10-01 | 2025-04-01 | North Carolina State University | Recombinant type I CRISPR-Cas system and uses thereof for genome modification and alteration of expression |
| US10711267B2 (en) | 2018-10-01 | 2020-07-14 | North Carolina State University | Recombinant type I CRISPR-Cas system |
| WO2020072254A1 (en) | 2018-10-01 | 2020-04-09 | North Carolina State University | Recombinant type i crispr-cas system and uses thereof for killing target cells |
| US12281338B2 (en) | 2018-10-29 | 2025-04-22 | The Broad Institute, Inc. | Nucleobase editors comprising GeoCas9 and uses thereof |
| WO2020127332A1 (en) * | 2018-12-17 | 2020-06-25 | Consejo Superior De Investigaciones Científicas (Csic) | Method for the introduction of genetic information in cell by site-specific integration system |
| WO2020154500A1 (en) | 2019-01-23 | 2020-07-30 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| MX2021011426A (es) | 2019-03-19 | 2022-03-11 | Broad Inst Inc | Metodos y composiciones para editar secuencias de nucleótidos. |
| EP3956349A1 (en) | 2019-04-17 | 2022-02-23 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US10907125B2 (en) | 2019-06-20 | 2021-02-02 | Inscripta, Inc. | Flow through electroporation modules and instrumentation |
| CN114008070A (zh) | 2019-06-21 | 2022-02-01 | 因思科瑞普特公司 | 导致大肠杆菌赖氨酸产量增加的全基因组合理设计的突变 |
| US10927385B2 (en) | 2019-06-25 | 2021-02-23 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| US10689669B1 (en) | 2020-01-11 | 2020-06-23 | Inscripta, Inc. | Automated multi-module cell processing methods, instruments, and systems |
| CN115135370A (zh) | 2020-01-27 | 2022-09-30 | 因思科瑞普特公司 | 电穿孔模块和仪器 |
| US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
| MX2022014008A (es) | 2020-05-08 | 2023-02-09 | Broad Inst Inc | Métodos y composiciones para la edición simultánea de ambas cadenas de una secuencia de nucleótidos de doble cadena objetivo. |
| US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2001289104A1 (en) * | 2000-09-15 | 2002-03-26 | Deltagen, Inc. | Methods of producing cells and animals comprising targeted gene modifications |
| US7141425B2 (en) * | 2001-08-22 | 2006-11-28 | Maxcyte, Inc. | Apparatus and method for electroporation of biological samples |
| US20070059833A1 (en) * | 2005-09-07 | 2007-03-15 | Maxcyte, Inc. | Use of Nucleases to Improve Viability and Enhance Transgene Expression in Transfected Cells |
| CA2648778A1 (en) * | 2006-04-10 | 2007-10-18 | The Regents Of The University Of California | Method for culturing cells on removable pallets for subsequent cell expansion and analysis |
| AU2008262487B2 (en) * | 2007-05-23 | 2013-10-31 | Sangamo Therapeutics, Inc. | Methods and compositions for increased transgene expression |
| WO2012012738A1 (en) * | 2010-07-23 | 2012-01-26 | Sigma-Aldrich Co., Llc | Genome editing using targeting endonucleases and single-stranded nucleic acids |
| AU2012328682B2 (en) * | 2011-10-27 | 2017-09-21 | Sangamo Therapeutics, Inc. | Methods and compositions for modification of the HPRT locus |
| WO2013139994A1 (en) * | 2012-03-23 | 2013-09-26 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) | A novel method of producing an oocyte carrying a modified target sequence in its genome |
| EP2847338B1 (en) * | 2012-05-07 | 2018-09-19 | Sangamo Therapeutics, Inc. | Methods and compositions for nuclease-mediated targeted integration of transgenes |
| CN104540382A (zh) * | 2012-06-12 | 2015-04-22 | 弗·哈夫曼-拉罗切有限公司 | 用于产生条件性敲除等位基因的方法和组合物 |
| CN102839214A (zh) * | 2012-09-06 | 2012-12-26 | 许汉鹏 | 筛选真核细胞基因组内特定基因调控相关机制和分子的方法 |
| JP6683598B2 (ja) * | 2013-03-15 | 2020-04-22 | マックスサイト インコーポレーティッド | 安定なトランスフェクトされた細胞を生成するための方法および組成物 |
| EP3309248B1 (en) * | 2013-05-29 | 2021-06-09 | Cellectis | Methods for engineering t cells for immunotherapy by using rna-guided cas nuclease system |
-
2015
- 2015-04-13 EP EP15780038.4A patent/EP3132025B1/en active Active
- 2015-04-13 ES ES15780038T patent/ES2962509T3/es active Active
- 2015-04-13 SG SG11201608503UA patent/SG11201608503UA/en unknown
- 2015-04-13 CN CN201580025136.0A patent/CN106687585B/zh active Active
- 2015-04-13 KR KR1020167031636A patent/KR102406585B1/ko active Active
- 2015-04-13 JP JP2016562503A patent/JP2017513477A/ja active Pending
- 2015-04-13 WO PCT/US2015/025523 patent/WO2015160683A1/en not_active Ceased
- 2015-04-13 US US15/303,932 patent/US20170029805A1/en not_active Abandoned
-
2020
- 2020-07-29 JP JP2020127917A patent/JP7209671B2/ja active Active
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