JP2016539624A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2016539624A5 JP2016539624A5 JP2016521352A JP2016521352A JP2016539624A5 JP 2016539624 A5 JP2016539624 A5 JP 2016539624A5 JP 2016521352 A JP2016521352 A JP 2016521352A JP 2016521352 A JP2016521352 A JP 2016521352A JP 2016539624 A5 JP2016539624 A5 JP 2016539624A5
- Authority
- JP
- Japan
- Prior art keywords
- double
- nucleic acid
- mismatch
- target nucleic
- allele
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108700028369 Alleles Proteins 0.000 claims description 36
- 102000039446 nucleic acids Human genes 0.000 claims description 29
- 108020004707 nucleic acids Proteins 0.000 claims description 29
- 150000007523 nucleic acids Chemical class 0.000 claims description 29
- 108091034117 Oligonucleotide Proteins 0.000 claims description 24
- 108091033319 polynucleotide Proteins 0.000 claims description 24
- 102000040430 polynucleotide Human genes 0.000 claims description 24
- 239000002157 polynucleotide Substances 0.000 claims description 24
- 239000002773 nucleotide Substances 0.000 claims description 17
- 125000003729 nucleotide group Chemical group 0.000 claims description 17
- 150000001875 compounds Chemical class 0.000 claims description 15
- 230000002687 intercalation Effects 0.000 claims description 9
- 238000009830 intercalation Methods 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 239000011159 matrix material Substances 0.000 claims description 6
- 241000317914 Chrysis Species 0.000 claims description 5
- 238000003776 cleavage reaction Methods 0.000 claims description 5
- 230000000295 complement effect Effects 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 230000007017 scission Effects 0.000 claims description 5
- 238000009396 hybridization Methods 0.000 claims description 3
- 239000010948 rhodium Substances 0.000 claims 16
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 6
- 239000007801 affinity label Substances 0.000 claims 6
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 4
- 125000000217 alkyl group Chemical group 0.000 claims 4
- 125000003118 aryl group Chemical group 0.000 claims 4
- 102000052116 epidermal growth factor receptor activity proteins Human genes 0.000 claims 4
- 108700015053 epidermal growth factor receptor activity proteins Proteins 0.000 claims 4
- 239000001257 hydrogen Substances 0.000 claims 4
- 229910052739 hydrogen Inorganic materials 0.000 claims 4
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims 4
- YOHYSYJDKVYCJI-UHFFFAOYSA-N n-[3-[[6-[3-(trifluoromethyl)anilino]pyrimidin-4-yl]amino]phenyl]cyclopropanecarboxamide Chemical compound FC(F)(F)C1=CC=CC(NC=2N=CN=C(NC=3C=C(NC(=O)C4CC4)C=CC=3)C=2)=C1 YOHYSYJDKVYCJI-UHFFFAOYSA-N 0.000 claims 4
- 229910052703 rhodium Inorganic materials 0.000 claims 4
- MHOVAHRLVXNVSD-UHFFFAOYSA-N rhodium atom Chemical compound [Rh] MHOVAHRLVXNVSD-UHFFFAOYSA-N 0.000 claims 4
- 239000007787 solid Substances 0.000 claims 4
- 229910052757 nitrogen Inorganic materials 0.000 claims 3
- 239000011541 reaction mixture Substances 0.000 claims 2
- 230000003321 amplification Effects 0.000 claims 1
- 239000003054 catalyst Substances 0.000 claims 1
- 238000002372 labelling Methods 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361909587P | 2013-11-27 | 2013-11-27 | |
| US61/909,587 | 2013-11-27 | ||
| PCT/EP2014/075460 WO2015078831A1 (en) | 2013-11-27 | 2014-11-25 | Methods for the enrichment of mutated nucleic acid from a mixture |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016539624A JP2016539624A (ja) | 2016-12-22 |
| JP2016539624A5 true JP2016539624A5 (cg-RX-API-DMAC7.html) | 2019-02-28 |
| JP6553598B2 JP6553598B2 (ja) | 2019-07-31 |
Family
ID=52000821
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016521352A Active JP6553598B2 (ja) | 2013-11-27 | 2014-11-25 | 混合物から変異された核酸の富化方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9873908B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP3074529B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6553598B2 (cg-RX-API-DMAC7.html) |
| CN (1) | CN105745336B (cg-RX-API-DMAC7.html) |
| CA (1) | CA2926693C (cg-RX-API-DMAC7.html) |
| ES (1) | ES2676062T3 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2015078831A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2545189B1 (en) | 2010-03-08 | 2018-01-10 | Dana-Farber Cancer Institute, Inc. | Full cold-pcr enrichment with reference blocking sequence |
| US11130992B2 (en) | 2011-03-31 | 2021-09-28 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable multiplex COLD-PCR |
| PL3246416T3 (pl) | 2011-04-15 | 2024-09-30 | The Johns Hopkins University | Bezpieczny system sekwencjonowania |
| CA2889937C (en) | 2012-10-29 | 2020-12-29 | The Johns Hopkins University | Papanicolaou test for ovarian and endometrial cancers |
| JP6941568B2 (ja) | 2015-06-24 | 2021-09-29 | デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド | ヌクレアーゼを使用する野生型ｄｎａの選択的分解および突然変異体対立遺伝子の濃縮 |
| US11286531B2 (en) | 2015-08-11 | 2022-03-29 | The Johns Hopkins University | Assaying ovarian cyst fluid |
| WO2018111835A1 (en) | 2016-12-12 | 2018-06-21 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection |
| WO2019067092A1 (en) | 2017-08-07 | 2019-04-04 | The Johns Hopkins University | METHODS AND SUBSTANCES FOR THE EVALUATION AND TREATMENT OF CANCER |
| EP3746567A4 (en) * | 2018-02-26 | 2021-11-03 | CY Molecular Diagnostics, Inc | COMPOSITION AND PROCESSES FOR AFFINITY-DIRECTED ENRICHMENT OF RARE SPECIES |
| KR102589502B1 (ko) | 2018-07-19 | 2023-10-13 | 바이오피델리티 엘티디 | 개선된 폴리뉴클레오티드 서열 탐지 방법 |
| US20220073977A1 (en) | 2020-02-14 | 2022-03-10 | The Johns Hopkins University | Methods and materials for assessing nucleic acids |
| EP4143334A1 (en) | 2021-04-15 | 2023-03-08 | Biofidelity | Nucleic acid enrichment and detection |
| CN113774113B (zh) * | 2021-09-17 | 2023-07-04 | 广州医科大学附属第一医院（广州呼吸中心） | 一种富集突变基因序列的方法和应用 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6120992A (en) * | 1993-11-04 | 2000-09-19 | Valigene Corporation | Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification in a diseased human |
| WO1997034015A1 (en) | 1996-03-15 | 1997-09-18 | The Penn State Research Foundation | Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays |
| US6630301B1 (en) | 1997-03-14 | 2003-10-07 | The Penn State Research Foundation | Detection of extracellular tumor-associated nucleic acid in blood plasma or serum |
| US6031098A (en) | 1997-08-11 | 2000-02-29 | California Institute Of Technology | Detection and treatment of duplex polynucleotide damage |
| SE9902741D0 (sv) | 1999-07-19 | 1999-07-19 | Boerries Kemper | Method for the enrichment of heteroduplexes and its use in mutation detection |
| US20030082537A1 (en) * | 2000-05-23 | 2003-05-01 | Stanton Vincent P. | Methods for genetic analysis of DNA to detect sequence variances |
| US20020132244A1 (en) | 2000-09-27 | 2002-09-19 | Xiao-Cheng Li-Sucholeiki | Methods for detecting rare polymorphic variants in genomic DNA sequences |
| JP2004522739A (ja) * | 2000-12-08 | 2004-07-29 | カリフォルニア インスティテュート オブ テクノロジー | ポリヌクレオチド二本鎖損傷を検出するための方法および組成物 |
| WO2002086196A1 (en) * | 2001-04-19 | 2002-10-31 | Rd Chemical Company | Copper acid baths, system and method for electroplating high aspect ratio substrates |
| WO2002086169A1 (en) | 2001-04-23 | 2002-10-31 | Dana-Farber Cancer Institute, Inc. | Methods for rapid screening of polymorphisms, mutations and methylation |
| GB2382137A (en) | 2001-11-20 | 2003-05-21 | Mats Gullberg | Nucleic acid enrichment |
| CA2558753A1 (en) * | 2004-03-01 | 2005-09-15 | University Of Chicago | Polymorphisms in the epidermal growth factor receptor gene promoter |
| US8076082B2 (en) | 2005-10-14 | 2011-12-13 | Cleveland State University | Methods for identifying multiple DNA alteration markers in a large background of wild-type DNA |
| US20070154892A1 (en) | 2005-12-30 | 2007-07-05 | Simon Wain-Hobson | Differential amplification of mutant nucleic acids by PCR in a mixure of nucleic acids |
| US7786298B2 (en) | 2006-01-13 | 2010-08-31 | California Institute Of Technology | Compounds and methods for nucleic acid mismatch detection |
| EP2545189B1 (en) | 2010-03-08 | 2018-01-10 | Dana-Farber Cancer Institute, Inc. | Full cold-pcr enrichment with reference blocking sequence |
| US20120164641A1 (en) | 2010-12-22 | 2012-06-28 | Roche Molecular Systems, Inc. | Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene |
| CN103215361A (zh) * | 2013-04-18 | 2013-07-24 | 深圳联合医学科技有限公司 | 等位基因变体检测方法、试剂盒及组合物 |
-
2014
- 2014-11-19 US US14/547,899 patent/US9873908B2/en active Active
- 2014-11-25 JP JP2016521352A patent/JP6553598B2/ja active Active
- 2014-11-25 CA CA2926693A patent/CA2926693C/en active Active
- 2014-11-25 ES ES14805538.7T patent/ES2676062T3/es active Active
- 2014-11-25 CN CN201480064597.4A patent/CN105745336B/zh active Active
- 2014-11-25 WO PCT/EP2014/075460 patent/WO2015078831A1/en not_active Ceased
- 2014-11-25 EP EP14805538.7A patent/EP3074529B1/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2016539624A5 (cg-RX-API-DMAC7.html) | ||
| US11866780B2 (en) | Nucleic acid sample enrichment for sequencing applications | |
| AU2019384154B2 (en) | Methods for targeted nucleic acid library formation | |
| CA2810931C (en) | Direct capture, amplification and sequencing of target dna using immobilized primers | |
| JP4226476B2 (ja) | 環状化可能プローブを用いた複数の標的配列の分析と検出 | |
| US20150353993A1 (en) | Compositions, methods, systems and kits for target nucleic acid enrichment | |
| US20220127597A1 (en) | Haplotagging - haplotype phasing and single-tube combinatorial barcoding of nucleic acid molecules using bead-immobilized tn5 transposase | |
| US20120028814A1 (en) | Oligonucleotide ligation, barcoding and methods and compositions for improving data quality and throughput using massively parallel sequencing | |
| RU2019138698A (ru) | Способы и композиции для днк-профилирования | |
| US20150050657A1 (en) | Targeted enrichment and amplification of nucleic acids on a support | |
| US9822394B2 (en) | Nucleic acid sample preparation | |
| KR20170107970A (ko) | 무효소 및 무증폭 시퀀싱 | |
| EP1786916A4 (en) | ON PRIMER-nucleic acid amplification | |
| RU2013113407A (ru) | Способы улавливания и секвенирования нуклеиновых кислот | |
| JP2013509871A5 (cg-RX-API-DMAC7.html) | ||
| JP7539770B2 (ja) | ゲノム再編成検出のための配列決定方法 | |
| ATE393244T1 (de) | Verfahren zur genotypisierung durch schmelztemperaturabhängigkeit | |
| US20100112556A1 (en) | Method for sample analysis using q probes | |
| JP2016508713A5 (cg-RX-API-DMAC7.html) | ||
| RU2016104218A (ru) | Определение целевых нуклеиновокислотных последовательностей путем расщепления зондирующего олигонуклеотида и гибридизации | |
| JP2015533281A (ja) | 複数の異なる核酸標的配列を同時に増幅する方法 | |
| EP2785865A1 (en) | Method and kit for characterizing rna in a composition | |
| JP2010035532A (ja) | アレル特異的ｐｃｒ法 | |
| JP2008504826A5 (cg-RX-API-DMAC7.html) | ||
| US20150284788A1 (en) | Sequencing Performance With Additives |