ATE548469T1 - Verfahren zur identifizierung mehrerer mutationen oder methylierungsmarkern in einem dominierenden hintergrund von wildtyp-dna - Google Patents

Verfahren zur identifizierung mehrerer mutationen oder methylierungsmarkern in einem dominierenden hintergrund von wildtyp-dna

Info

Publication number
ATE548469T1
ATE548469T1 AT06816985T AT06816985T ATE548469T1 AT E548469 T1 ATE548469 T1 AT E548469T1 AT 06816985 T AT06816985 T AT 06816985T AT 06816985 T AT06816985 T AT 06816985T AT E548469 T1 ATE548469 T1 AT E548469T1
Authority
AT
Austria
Prior art keywords
wild
type dna
multiple mutations
identifying multiple
methylation markers
Prior art date
Application number
AT06816985T
Other languages
English (en)
Inventor
Baochuan Guo
Original Assignee
Univ State Cleveland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ State Cleveland filed Critical Univ State Cleveland
Application granted granted Critical
Publication of ATE548469T1 publication Critical patent/ATE548469T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT06816985T 2005-10-14 2006-10-13 Verfahren zur identifizierung mehrerer mutationen oder methylierungsmarkern in einem dominierenden hintergrund von wildtyp-dna ATE548469T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US72716805P 2005-10-14 2005-10-14
PCT/US2006/040357 WO2007047572A2 (en) 2005-10-14 2006-10-13 Methods for identifying multiple dna alteration markers in a large background of wild-type dna

Publications (1)

Publication Number Publication Date
ATE548469T1 true ATE548469T1 (de) 2012-03-15

Family

ID=37963165

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06816985T ATE548469T1 (de) 2005-10-14 2006-10-13 Verfahren zur identifizierung mehrerer mutationen oder methylierungsmarkern in einem dominierenden hintergrund von wildtyp-dna

Country Status (5)

Country Link
US (2) US8076082B2 (de)
EP (1) EP1951901B1 (de)
CN (1) CN101370945B (de)
AT (1) ATE548469T1 (de)
WO (1) WO2007047572A2 (de)

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Publication number Priority date Publication date Assignee Title
JP2008136404A (ja) * 2006-11-30 2008-06-19 Sysmex Corp Ｄｎａメチル化検出における非メチル化シトシン変換処理後のｄｎａ量の確認方法
JP5795341B2 (ja) 2010-03-08 2015-10-14 デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド 参照ブロック配列によるｆｕｌｌＣＯＬＤ−ＰＣＲ濃縮
CN103328654B (zh) 2010-10-27 2017-07-11 哈佛学院院长等 立足点引物双链体的组合物及其使用方法
EP2691541B1 (de) * 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Verfahren zur erhöhung der konzentration an einzelsträngige mutantsequenzen in einer mischung aus wildtyp- und mutantsequenzen
WO2012151328A2 (en) 2011-05-02 2012-11-08 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
US9617392B2 (en) 2011-07-10 2017-04-11 President And Fellows Of Harvard College Compositions and methods for self-assembly of polymers with complementary macroscopic and microscopic scale units
US9279146B2 (en) 2012-12-21 2016-03-08 Roche Molecular Systems, Inc. Compounds and methods for the enrichment of mutated nucleic acid from a mixture
EP3068904A4 (de) * 2013-11-11 2017-11-15 The Translational Genomics Research Institute Systeme und verfahren für universelle anhangsbasierte indexierungsstrategien zur amplicon-sequenzierung
US9873908B2 (en) 2013-11-27 2018-01-23 Roche Molecular Systems, Inc. Methods for the enrichment of mutated nucleic acid from a mixture
US10480021B2 (en) * 2014-06-23 2019-11-19 Yale University Methods for closed chromatin mapping and DNA methylation analysis for single cells
CN105463074B (zh) * 2015-11-18 2018-10-16 扬州大学 一种快速测定脱氧核糖核酸甲基化率的方法
CN106011230A (zh) * 2016-05-10 2016-10-12 人和未来生物科技（长沙）有限公司 用于检测碎片化dna目标区域的引物组合物及其应用
CN109642230A (zh) 2016-08-16 2019-04-16 加利福尼亚大学董事会 通过杂交找到低丰度序列(flash)的方法
KR102006803B1 (ko) * 2016-10-06 2019-08-05 (주)지노믹트리 메틸화 dna 다중 검출방법
CN107012221A (zh) * 2016-10-14 2017-08-04 苏州艾达康医疗科技有限公司 基于阻断物的富集系统及其应用
CA3046953A1 (en) 2016-12-12 2018-06-21 Dana Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
CN106755443B (zh) * 2016-12-30 2018-11-30 人和未来生物科技（长沙）有限公司 一种从多种混合dna中超灵敏检测痕量dna的方法和试剂
US20180237853A1 (en) * 2017-02-19 2018-08-23 Yan Wang Methods, Compositions and Kits for Detection of Mutant Variants of Target Genes
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE
CN108998504A (zh) * 2018-08-08 2018-12-14 鲍伟胜 一种提高基因突变检测灵敏度的方法
WO2020050350A1 (ja) * 2018-09-07 2020-03-12 株式会社ヘルスケアシステムズ ヒトパピローマウイルスの検出方法
CN111996244B (zh) * 2020-10-28 2022-05-06 浙江绍兴鼎晶生物医药科技股份有限公司 一种单核苷酸多态性检测用组合物及其应用
CN115161408A (zh) * 2022-05-25 2022-10-11 华中农业大学 玉米基因组目标区段的dna甲基化检测

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5834181A (en) * 1994-07-28 1998-11-10 Genzyme Corporation High throughput screening method for sequences or genetic alterations in nucleic acids
US7611869B2 (en) * 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US20020132244A1 (en) 2000-09-27 2002-09-19 Xiao-Cheng Li-Sucholeiki Methods for detecting rare polymorphic variants in genomic DNA sequences
WO2003006677A2 (en) * 2001-07-12 2003-01-23 Illumina, Inc. Multiplex nucleic acid reactions
US7223538B2 (en) * 2001-12-14 2007-05-29 Ge Healthcare Bio-Sciences Ab Post-synthesis labeling of nucleic acids, assays using nucleic acids that are labeled post-synthetically, single nucleotide polymorphism detection, and associated compounds and microarrays
EP1620536A4 (de) * 2003-03-12 2007-09-26 Univ State Cleveland Molekulare haplotypisierung genomischer dna
US20050009059A1 (en) * 2003-05-07 2005-01-13 Affymetrix, Inc. Analysis of methylation status using oligonucleotide arrays
US7358048B2 (en) 2004-02-10 2008-04-15 Cornell Research Foundation, Inc. Method for detection of promoter methylation status

Also Published As

Publication number Publication date
EP1951901A4 (de) 2010-01-20
EP1951901B1 (de) 2012-03-07
WO2007047572A3 (en) 2007-11-15
WO2007047572A2 (en) 2007-04-26
CN101370945B (zh) 2011-12-28
EP1951901A2 (de) 2008-08-06
US20090042196A1 (en) 2009-02-12
US20120252013A1 (en) 2012-10-04
US8076082B2 (en) 2011-12-13
CN101370945A (zh) 2009-02-18

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