JP2016535987A - 核酸配列の合成及び濃縮 - Google Patents

核酸配列の合成及び濃縮 Download PDF

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JP2016535987A
JP2016535987A JP2016524530A JP2016524530A JP2016535987A JP 2016535987 A JP2016535987 A JP 2016535987A JP 2016524530 A JP2016524530 A JP 2016524530A JP 2016524530 A JP2016524530 A JP 2016524530A JP 2016535987 A JP2016535987 A JP 2016535987A
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sequence
target
temperature
blocker
melting temperature
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JP2016535987A5 (enExample
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プール、ジェイソン
ハンコック、ゼーゲ
コスコ、カレナ
メルニコワ、ヴラダ
クラウチャー、ピーター
ル、ティム
エルランデル、マーク、ジー．
サミュエルズ、エリン
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トローバジーン インコーポレイテッド
トローバジーン インコーポレイテッド
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Priority claimed from PCT/US2014/061435 external-priority patent/WO2015073163A2/en
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Publication of JP2016535987A5 publication Critical patent/JP2016535987A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2016524530A 2013-10-20 2014-10-20 核酸配列の合成及び濃縮 Withdrawn JP2016535987A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201361893283P 2013-10-20 2013-10-20
US61/893,283 2013-10-20
US201361904141P 2013-11-14 2013-11-14
US61/904,141 2013-11-14
US201462039905P 2014-08-20 2014-08-20
US62/039,905 2014-08-20
PCT/US2014/061435 WO2015073163A2 (en) 2013-11-14 2014-10-20 Synthesis and enrichment of nucleic acid sequences

Publications (2)

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JP2016535987A true JP2016535987A (ja) 2016-11-24
JP2016535987A5 JP2016535987A5 (enExample) 2017-11-30

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US (2) US20160273022A1 (enExample)
JP (1) JP2016535987A (enExample)
CN (1) CN105283555A (enExample)

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EP4414990A3 (en) 2013-01-17 2024-11-06 Personalis, Inc. Methods and systems for genetic analysis
EP3212808B1 (en) 2014-10-30 2022-03-02 Personalis, Inc. Methods for using mosaicism in nucleic acids sampled distal to their origin
CA2984960C (en) * 2015-05-18 2022-10-04 Saga Diagnostics Ab Detection of target nucleic acid and variants
US11299783B2 (en) 2016-05-27 2022-04-12 Personalis, Inc. Methods and systems for genetic analysis
CN107012221A (zh) * 2016-10-14 2017-08-04 苏州艾达康医疗科技有限公司 基于阻断物的富集系统及其应用
CN106755451A (zh) * 2017-01-05 2017-05-31 苏州艾达康医疗科技有限公司 核酸制备及分析
EP3660168A4 (en) * 2017-07-26 2021-04-21 Sekisui Medical Co., Ltd. METHOD OF DETECTING MUTUAL GENES
CN108179191A (zh) * 2018-02-05 2018-06-19 广州市达瑞生物技术股份有限公司 一种检测人类ctDNA基因突变的试剂盒
CN108410982A (zh) * 2018-02-05 2018-08-17 广州市达瑞生物技术股份有限公司 一种基于maldi-tof-ms检测肺癌34个突变位点的试剂盒
US10801064B2 (en) 2018-05-31 2020-10-13 Personalis, Inc. Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples
US11814750B2 (en) 2018-05-31 2023-11-14 Personalis, Inc. Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples
CN109632938B (zh) * 2019-01-11 2021-03-16 中国科学院化学研究所 聚多巴胺修饰的银纳米颗粒在质谱分析检测中的应用
CN109762901B (zh) * 2019-01-30 2022-11-29 杭州瑞普基因科技有限公司 用于富集低频dna突变的dna探针应用于多种突变的同时检测
CN114945987A (zh) 2019-11-05 2022-08-26 佩索纳里斯公司 从单一样品预估肿瘤纯度
CN111004839A (zh) * 2019-12-18 2020-04-14 杭州瑞普基因科技有限公司 基于锁核酸修饰的阻滞物进行阻滞取代扩增富集检测目标突变的方法
US12297508B2 (en) 2021-10-05 2025-05-13 Personalis, Inc. Customized assays for personalized cancer monitoring
CN115287338A (zh) * 2022-07-04 2022-11-04 苏州唯善生物科技有限公司 一种提高数字pcr灵敏度的检测方法

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US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
US6391592B1 (en) * 2000-12-14 2002-05-21 Affymetrix, Inc. Blocker-aided target amplification of nucleic acids
AUPS076902A0 (en) 2002-02-26 2002-03-21 Commonwealth Scientific And Industrial Research Organisation Novel selective polymerase chain reaction
ES2546848T3 (es) * 2006-03-10 2015-09-29 Epigenomics Ag Un método para identificar una muestra biológica para el análisis de la metilación
AU2008282780B2 (en) * 2007-08-01 2014-04-17 Dana- Farber Cancer Institute Enrichment of a target sequence
ES2595489T3 (es) 2008-07-18 2016-12-30 Trovagene, Inc. Métodos para la detección de secuencias de ácidos nucleicos "ultracortos" basados en PCR
US20130178383A1 (en) * 2008-11-12 2013-07-11 David Spetzler Vesicle isolation methods
US8206929B2 (en) 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
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KR101312241B1 (ko) 2010-04-27 2013-09-27 사회복지법인 삼성생명공익재단 증폭억제시발체를 이용하는 유전자 돌연변이 검출 방법
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US20170009276A1 (en) 2017-01-12
US9725755B2 (en) 2017-08-08
US20160273022A1 (en) 2016-09-22
CN105283555A (zh) 2016-01-27

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