CN105283555A - 核酸序列的合成和富集 - Google Patents
核酸序列的合成和富集 Download PDFInfo
- Publication number
- CN105283555A CN105283555A CN201480013111.4A CN201480013111A CN105283555A CN 105283555 A CN105283555 A CN 105283555A CN 201480013111 A CN201480013111 A CN 201480013111A CN 105283555 A CN105283555 A CN 105283555A
- Authority
- CN
- China
- Prior art keywords
- sequence
- reference sequences
- blocker
- temperature
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000007523 nucleic acids Chemical group 0.000 title claims abstract description 84
- 230000015572 biosynthetic process Effects 0.000 title description 8
- 238000003786 synthesis reaction Methods 0.000 title description 7
- 238000000034 method Methods 0.000 claims abstract description 138
- 230000008859 change Effects 0.000 claims description 102
- 238000002844 melting Methods 0.000 claims description 84
- 230000008018 melting Effects 0.000 claims description 81
- 102000039446 nucleic acids Human genes 0.000 claims description 59
- 108020004707 nucleic acids Proteins 0.000 claims description 59
- 239000002773 nucleotide Substances 0.000 claims description 51
- 125000003729 nucleotide group Chemical group 0.000 claims description 51
- 239000011541 reaction mixture Substances 0.000 claims description 50
- 230000000295 complement effect Effects 0.000 claims description 32
- 230000000903 blocking effect Effects 0.000 claims description 30
- 238000004925 denaturation Methods 0.000 claims description 30
- 230000036425 denaturation Effects 0.000 claims description 30
- 102100030708 GTPase KRas Human genes 0.000 claims description 24
- 230000004087 circulation Effects 0.000 claims description 18
- 238000000137 annealing Methods 0.000 claims description 16
- 230000035772 mutation Effects 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 11
- 108700028369 Alleles Proteins 0.000 claims description 10
- 101000984753 Homo sapiens Serine/threonine-protein kinase B-raf Proteins 0.000 claims description 10
- 102100027103 Serine/threonine-protein kinase B-raf Human genes 0.000 claims description 10
- 230000027455 binding Effects 0.000 claims description 10
- 102000052116 epidermal growth factor receptor activity proteins Human genes 0.000 claims description 10
- 108700015053 epidermal growth factor receptor activity proteins Proteins 0.000 claims description 10
- YOHYSYJDKVYCJI-UHFFFAOYSA-N n-[3-[[6-[3-(trifluoromethyl)anilino]pyrimidin-4-yl]amino]phenyl]cyclopropanecarboxamide Chemical compound FC(F)(F)C1=CC=CC(NC=2N=CN=C(NC=3C=C(NC(=O)C4CC4)C=CC=3)C=2)=C1 YOHYSYJDKVYCJI-UHFFFAOYSA-N 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 6
- 238000012163 sequencing technique Methods 0.000 claims description 6
- 238000003753 real-time PCR Methods 0.000 claims description 5
- 102100039788 GTPase NRas Human genes 0.000 claims description 4
- 101000744505 Homo sapiens GTPase NRas Proteins 0.000 claims description 4
- 101001012157 Homo sapiens Receptor tyrosine-protein kinase erbB-2 Proteins 0.000 claims description 4
- 102100030086 Receptor tyrosine-protein kinase erbB-2 Human genes 0.000 claims description 4
- 230000002829 reductive effect Effects 0.000 claims description 4
- 101100322915 Caenorhabditis elegans akt-1 gene Proteins 0.000 claims description 3
- ZEOWTGPWHLSLOG-UHFFFAOYSA-N Cc1ccc(cc1-c1ccc2c(n[nH]c2c1)-c1cnn(c1)C1CC1)C(=O)Nc1cccc(c1)C(F)(F)F Chemical compound Cc1ccc(cc1-c1ccc2c(n[nH]c2c1)-c1cnn(c1)C1CC1)C(=O)Nc1cccc(c1)C(F)(F)F ZEOWTGPWHLSLOG-UHFFFAOYSA-N 0.000 claims description 3
- 102100025064 Cellular tumor antigen p53 Human genes 0.000 claims description 3
- 102100023593 Fibroblast growth factor receptor 1 Human genes 0.000 claims description 3
- 101710182386 Fibroblast growth factor receptor 1 Proteins 0.000 claims description 3
- 102100023600 Fibroblast growth factor receptor 2 Human genes 0.000 claims description 3
- 101710182389 Fibroblast growth factor receptor 2 Proteins 0.000 claims description 3
- 102100027842 Fibroblast growth factor receptor 3 Human genes 0.000 claims description 3
- 101710182396 Fibroblast growth factor receptor 3 Proteins 0.000 claims description 3
- 101000605639 Homo sapiens Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Proteins 0.000 claims description 3
- 101000829171 Hypocrea virens (strain Gv29-8 / FGSC 10586) Effector TSP1 Proteins 0.000 claims description 3
- 102100025744 Mothers against decapentaplegic homolog 1 Human genes 0.000 claims description 3
- 102100038332 Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Human genes 0.000 claims description 3
- 108010051742 Platelet-Derived Growth Factor beta Receptor Proteins 0.000 claims description 3
- 102100026547 Platelet-derived growth factor receptor beta Human genes 0.000 claims description 3
- 101700032040 SMAD1 Proteins 0.000 claims description 3
- 108010006785 Taq Polymerase Proteins 0.000 claims description 3
- 108010078814 Tumor Suppressor Protein p53 Proteins 0.000 claims description 3
- 230000001351 cycling effect Effects 0.000 claims description 3
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 claims description 3
- 235000011180 diphosphates Nutrition 0.000 claims description 3
- 230000001036 exonucleolytic effect Effects 0.000 claims description 3
- 230000000977 initiatory effect Effects 0.000 claims description 3
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 claims description 3
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 claims description 3
- 108700024394 Exon Proteins 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract description 28
- 238000001514 detection method Methods 0.000 abstract description 21
- 230000035945 sensitivity Effects 0.000 abstract description 9
- 239000013615 primer Substances 0.000 description 91
- 239000000523 sample Substances 0.000 description 59
- 230000003321 amplification Effects 0.000 description 44
- 238000003199 nucleic acid amplification method Methods 0.000 description 44
- 206010028980 Neoplasm Diseases 0.000 description 27
- 108020004414 DNA Proteins 0.000 description 26
- 201000011510 cancer Diseases 0.000 description 23
- 101000584612 Homo sapiens GTPase KRas Proteins 0.000 description 22
- 108091034117 Oligonucleotide Proteins 0.000 description 22
- 238000003752 polymerase chain reaction Methods 0.000 description 18
- 210000004027 cell Anatomy 0.000 description 14
- 238000006243 chemical reaction Methods 0.000 description 13
- 210000002700 urine Anatomy 0.000 description 13
- 238000013461 design Methods 0.000 description 12
- 238000012360 testing method Methods 0.000 description 12
- 210000001519 tissue Anatomy 0.000 description 11
- 108091093088 Amplicon Proteins 0.000 description 10
- 108091027305 Heteroduplex Proteins 0.000 description 10
- -1 c-MET Proteins 0.000 description 10
- 238000009826 distribution Methods 0.000 description 9
- 238000012408 PCR amplification Methods 0.000 description 8
- 210000004369 blood Anatomy 0.000 description 8
- 239000008280 blood Substances 0.000 description 8
- 230000000875 corresponding effect Effects 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 8
- 239000012472 biological sample Substances 0.000 description 7
- 239000002253 acid Substances 0.000 description 6
- 210000001124 body fluid Anatomy 0.000 description 6
- 239000010839 body fluid Substances 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 230000000670 limiting effect Effects 0.000 description 6
- 108091093037 Peptide nucleic acid Proteins 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 239000000975 dye Substances 0.000 description 5
- 210000002381 plasma Anatomy 0.000 description 5
- 210000000952 spleen Anatomy 0.000 description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 4
- 230000010558 Gene Alterations Effects 0.000 description 4
- 206010025323 Lymphomas Diseases 0.000 description 4
- 241000124008 Mammalia Species 0.000 description 4
- 239000013068 control sample Substances 0.000 description 4
- 230000008034 disappearance Effects 0.000 description 4
- 238000006062 fragmentation reaction Methods 0.000 description 4
- 208000032839 leukemia Diseases 0.000 description 4
- 108091033319 polynucleotide Proteins 0.000 description 4
- 102000040430 polynucleotide Human genes 0.000 description 4
- 239000002157 polynucleotide Substances 0.000 description 4
- 230000002441 reversible effect Effects 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 206010062113 splenic marginal zone lymphoma Diseases 0.000 description 4
- 208000010839 B-cell chronic lymphocytic leukemia Diseases 0.000 description 3
- 206010009944 Colon cancer Diseases 0.000 description 3
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- 206010062717 Increased upper airway secretion Diseases 0.000 description 3
- 208000015914 Non-Hodgkin lymphomas Diseases 0.000 description 3
- 108020004682 Single-Stranded DNA Proteins 0.000 description 3
- 239000012491 analyte Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000013467 fragmentation Methods 0.000 description 3
- 238000009396 hybridization Methods 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 210000003097 mucus Anatomy 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 210000001819 pancreatic juice Anatomy 0.000 description 3
- 208000026435 phlegm Diseases 0.000 description 3
- 238000012207 quantitative assay Methods 0.000 description 3
- 230000035484 reaction time Effects 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 210000003296 saliva Anatomy 0.000 description 3
- 210000000582 semen Anatomy 0.000 description 3
- 210000001138 tear Anatomy 0.000 description 3
- DSGKWFGEUBCEIE-UHFFFAOYSA-N (2-carbonochloridoylphenyl) acetate Chemical compound CC(=O)OC1=CC=CC=C1C(Cl)=O DSGKWFGEUBCEIE-UHFFFAOYSA-N 0.000 description 2
- 208000031261 Acute myeloid leukaemia Diseases 0.000 description 2
- 208000003950 B-cell lymphoma Diseases 0.000 description 2
- 201000009030 Carcinoma Diseases 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 208000012468 Ewing sarcoma/peripheral primitive neuroectodermal tumor Diseases 0.000 description 2
- 208000031852 Gastrointestinal stromal cancer Diseases 0.000 description 2
- 101150105104 Kras gene Proteins 0.000 description 2
- 206010057444 Oropharyngeal neoplasm Diseases 0.000 description 2
- 208000033766 Prolymphocytic Leukemia Diseases 0.000 description 2
- 206010039491 Sarcoma Diseases 0.000 description 2
- 201000008736 Systemic mastocytosis Diseases 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 210000004381 amniotic fluid Anatomy 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 2
- 201000010989 colorectal carcinoma Diseases 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 230000001934 delay Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 210000002768 hair cell Anatomy 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 201000002313 intestinal cancer Diseases 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000036210 malignancy Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 201000006958 oropharynx cancer Diseases 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 201000000849 skin cancer Diseases 0.000 description 2
- 201000010106 skin squamous cell carcinoma Diseases 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 238000002626 targeted therapy Methods 0.000 description 2
- 208000024893 Acute lymphoblastic leukemia Diseases 0.000 description 1
- 208000007860 Anus Neoplasms Diseases 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 208000032791 BCR-ABL1 positive chronic myelogenous leukemia Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 206010005003 Bladder cancer Diseases 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 241000282836 Camelus dromedarius Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 206010007279 Carcinoid tumour of the gastrointestinal tract Diseases 0.000 description 1
- 208000017897 Carcinoma of esophagus Diseases 0.000 description 1
- 206010008342 Cervix carcinoma Diseases 0.000 description 1
- 108020001019 DNA Primers Proteins 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010014759 Endometrial neoplasm Diseases 0.000 description 1
- 241000283073 Equus caballus Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 201000003741 Gastrointestinal carcinoma Diseases 0.000 description 1
- 206010064571 Gene mutation Diseases 0.000 description 1
- 208000017604 Hodgkin disease Diseases 0.000 description 1
- 208000010747 Hodgkins lymphoma Diseases 0.000 description 1
- 206010021042 Hypopharyngeal cancer Diseases 0.000 description 1
- 206010056305 Hypopharyngeal neoplasm Diseases 0.000 description 1
- 208000005016 Intestinal Neoplasms Diseases 0.000 description 1
- 208000007766 Kaposi sarcoma Diseases 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- 206010023825 Laryngeal cancer Diseases 0.000 description 1
- 206010061523 Lip and/or oral cavity cancer Diseases 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 208000032298 Lymphoma cutis Diseases 0.000 description 1
- 208000032271 Malignant tumor of penis Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000034578 Multiple myelomas Diseases 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 206010028767 Nasal sinus cancer Diseases 0.000 description 1
- 208000002454 Nasopharyngeal Carcinoma Diseases 0.000 description 1
- 206010061306 Nasopharyngeal cancer Diseases 0.000 description 1
- 206010029260 Neuroblastoma Diseases 0.000 description 1
- 206010030155 Oesophageal carcinoma Diseases 0.000 description 1
- 206010033128 Ovarian cancer Diseases 0.000 description 1
- 206010061535 Ovarian neoplasm Diseases 0.000 description 1
- 208000003937 Paranasal Sinus Neoplasms Diseases 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 208000002471 Penile Neoplasms Diseases 0.000 description 1
- 206010034299 Penile cancer Diseases 0.000 description 1
- 241000009328 Perro Species 0.000 description 1
- 208000007913 Pituitary Neoplasms Diseases 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- 206010060862 Prostate cancer Diseases 0.000 description 1
- 208000000236 Prostatic Neoplasms Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 208000015634 Rectal Neoplasms Diseases 0.000 description 1
- 201000000582 Retinoblastoma Diseases 0.000 description 1
- 206010061934 Salivary gland cancer Diseases 0.000 description 1
- 208000000453 Skin Neoplasms Diseases 0.000 description 1
- 206010041067 Small cell lung cancer Diseases 0.000 description 1
- 206010054184 Small intestine carcinoma Diseases 0.000 description 1
- 208000021712 Soft tissue sarcoma Diseases 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 201000009365 Thymic carcinoma Diseases 0.000 description 1
- 208000033781 Thyroid carcinoma Diseases 0.000 description 1
- 208000024770 Thyroid neoplasm Diseases 0.000 description 1
- 208000007097 Urinary Bladder Neoplasms Diseases 0.000 description 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 1
- 201000005969 Uveal melanoma Diseases 0.000 description 1
- 201000003761 Vaginal carcinoma Diseases 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 208000033559 Waldenström macroglobulinemia Diseases 0.000 description 1
- 208000008383 Wilms tumor Diseases 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 208000020990 adrenal cortex carcinoma Diseases 0.000 description 1
- 208000007128 adrenocortical carcinoma Diseases 0.000 description 1
- 150000001413 amino acids Chemical group 0.000 description 1
- 201000011165 anus cancer Diseases 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 210000000270 basal cell Anatomy 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000002902 bimodal effect Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 201000010881 cervical cancer Diseases 0.000 description 1
- 208000006990 cholangiocarcinoma Diseases 0.000 description 1
- 201000010902 chronic myelomonocytic leukemia Diseases 0.000 description 1
- 210000003040 circulating cell Anatomy 0.000 description 1
- 239000002872 contrast media Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000001739 density measurement Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 201000003914 endometrial carcinoma Diseases 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 201000005619 esophageal carcinoma Diseases 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 208000021045 exocrine pancreatic carcinoma Diseases 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 201000010175 gallbladder cancer Diseases 0.000 description 1
- 201000007487 gallbladder carcinoma Diseases 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 208000013010 hypopharyngeal carcinoma Diseases 0.000 description 1
- 201000006866 hypopharynx cancer Diseases 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 208000022013 kidney Wilms tumor Diseases 0.000 description 1
- 201000005264 laryngeal carcinoma Diseases 0.000 description 1
- 210000000867 larynx Anatomy 0.000 description 1
- 208000012987 lip and oral cavity carcinoma Diseases 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 201000006385 lung benign neoplasm Diseases 0.000 description 1
- 201000005202 lung cancer Diseases 0.000 description 1
- 208000020816 lung neoplasm Diseases 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 201000000564 macroglobulinemia Diseases 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 201000001441 melanoma Diseases 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 238000009126 molecular therapy Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000002969 morbid Effects 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 208000018795 nasal cavity and paranasal sinus carcinoma Diseases 0.000 description 1
- 201000007425 nasal cavity carcinoma Diseases 0.000 description 1
- 201000011216 nasopharynx carcinoma Diseases 0.000 description 1
- 201000008026 nephroblastoma Diseases 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 208000002154 non-small cell lung carcinoma Diseases 0.000 description 1
- 201000005443 oral cavity cancer Diseases 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 201000008968 osteosarcoma Diseases 0.000 description 1
- 201000007052 paranasal sinus cancer Diseases 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 230000000505 pernicious effect Effects 0.000 description 1
- 208000010916 pituitary tumor Diseases 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 102000016914 ras Proteins Human genes 0.000 description 1
- 206010038038 rectal cancer Diseases 0.000 description 1
- 201000001275 rectum cancer Diseases 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000008261 resistance mechanism Effects 0.000 description 1
- 201000009410 rhabdomyosarcoma Diseases 0.000 description 1
- 125000000548 ribosyl group Chemical group C1([C@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 102200006532 rs112445441 Human genes 0.000 description 1
- 102200006531 rs121913529 Human genes 0.000 description 1
- 102200006537 rs121913529 Human genes 0.000 description 1
- 102200006539 rs121913529 Human genes 0.000 description 1
- 102200006538 rs121913530 Human genes 0.000 description 1
- 102200006540 rs121913530 Human genes 0.000 description 1
- 102200006541 rs121913530 Human genes 0.000 description 1
- 201000003804 salivary gland carcinoma Diseases 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 201000008261 skin carcinoma Diseases 0.000 description 1
- 208000000587 small cell lung carcinoma Diseases 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 230000005477 standard model Effects 0.000 description 1
- 201000000498 stomach carcinoma Diseases 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 208000008732 thymoma Diseases 0.000 description 1
- 201000002510 thyroid cancer Diseases 0.000 description 1
- 208000013077 thyroid gland carcinoma Diseases 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 230000017105 transposition Effects 0.000 description 1
- 208000029729 tumor suppressor gene on chromosome 11 Diseases 0.000 description 1
- 201000005112 urinary bladder cancer Diseases 0.000 description 1
- 208000012991 uterine carcinoma Diseases 0.000 description 1
- 201000003365 uterine corpus sarcoma Diseases 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361893283P | 2013-10-20 | 2013-10-20 | |
| US61/893,283 | 2013-10-20 | ||
| US201361904141P | 2013-11-14 | 2013-11-14 | |
| US61/904,141 | 2013-11-14 | ||
| US201462039905P | 2014-08-20 | 2014-08-20 | |
| US62/039,905 | 2014-08-20 | ||
| PCT/US2014/061435 WO2015073163A2 (en) | 2013-11-14 | 2014-10-20 | Synthesis and enrichment of nucleic acid sequences |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105283555A true CN105283555A (zh) | 2016-01-27 |
Family
ID=55151140
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201480013111.4A Pending CN105283555A (zh) | 2013-10-20 | 2014-10-20 | 核酸序列的合成和富集 |
Country Status (3)
| Country | Link |
|---|---|
| US (2) | US20160273022A1 (enExample) |
| JP (1) | JP2016535987A (enExample) |
| CN (1) | CN105283555A (enExample) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106755451A (zh) * | 2017-01-05 | 2017-05-31 | 苏州艾达康医疗科技有限公司 | 核酸制备及分析 |
| CN107012221A (zh) * | 2016-10-14 | 2017-08-04 | 苏州艾达康医疗科技有限公司 | 基于阻断物的富集系统及其应用 |
| CN108179191A (zh) * | 2018-02-05 | 2018-06-19 | 广州市达瑞生物技术股份有限公司 | 一种检测人类ctDNA基因突变的试剂盒 |
| CN108410982A (zh) * | 2018-02-05 | 2018-08-17 | 广州市达瑞生物技术股份有限公司 | 一种基于maldi-tof-ms检测肺癌34个突变位点的试剂盒 |
| CN109762901A (zh) * | 2019-01-30 | 2019-05-17 | 杭州瑞普基因科技有限公司 | 用于富集低频dna突变的dna探针应用于多种突变的同时检测 |
| CN111004839A (zh) * | 2019-12-18 | 2020-04-14 | 杭州瑞普基因科技有限公司 | 基于锁核酸修饰的阻滞物进行阻滞取代扩增富集检测目标突变的方法 |
| CN115287338A (zh) * | 2022-07-04 | 2022-11-04 | 苏州唯善生物科技有限公司 | 一种提高数字pcr灵敏度的检测方法 |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4414990A3 (en) | 2013-01-17 | 2024-11-06 | Personalis, Inc. | Methods and systems for genetic analysis |
| EP3212808B1 (en) | 2014-10-30 | 2022-03-02 | Personalis, Inc. | Methods for using mosaicism in nucleic acids sampled distal to their origin |
| CA2984960C (en) * | 2015-05-18 | 2022-10-04 | Saga Diagnostics Ab | Detection of target nucleic acid and variants |
| US11299783B2 (en) | 2016-05-27 | 2022-04-12 | Personalis, Inc. | Methods and systems for genetic analysis |
| EP3660168A4 (en) * | 2017-07-26 | 2021-04-21 | Sekisui Medical Co., Ltd. | METHOD OF DETECTING MUTUAL GENES |
| US10801064B2 (en) | 2018-05-31 | 2020-10-13 | Personalis, Inc. | Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples |
| US11814750B2 (en) | 2018-05-31 | 2023-11-14 | Personalis, Inc. | Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples |
| CN109632938B (zh) * | 2019-01-11 | 2021-03-16 | 中国科学院化学研究所 | 聚多巴胺修饰的银纳米颗粒在质谱分析检测中的应用 |
| CN114945987A (zh) | 2019-11-05 | 2022-08-26 | 佩索纳里斯公司 | 从单一样品预估肿瘤纯度 |
| US12297508B2 (en) | 2021-10-05 | 2025-05-13 | Personalis, Inc. | Customized assays for personalized cancer monitoring |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020098510A1 (en) * | 2000-12-14 | 2002-07-25 | Affymetrix, Inc. | Blocker-aided target amplification of nucleic acids |
| CN101815789A (zh) * | 2007-08-01 | 2010-08-25 | 达纳-法伯癌症研究院有限公司 | 靶序列的富集 |
| CN102859003A (zh) * | 2010-03-08 | 2013-01-02 | 达纳-法伯癌症研究院有限公司 | 使用参考封闭序列的全cold-pcr富集 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5849497A (en) * | 1997-04-03 | 1998-12-15 | The Research Foundation Of State University Of New York | Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker |
| AUPS076902A0 (en) | 2002-02-26 | 2002-03-21 | Commonwealth Scientific And Industrial Research Organisation | Novel selective polymerase chain reaction |
| ES2546848T3 (es) * | 2006-03-10 | 2015-09-29 | Epigenomics Ag | Un método para identificar una muestra biológica para el análisis de la metilación |
| ES2595489T3 (es) | 2008-07-18 | 2016-12-30 | Trovagene, Inc. | Métodos para la detección de secuencias de ácidos nucleicos "ultracortos" basados en PCR |
| US20130178383A1 (en) * | 2008-11-12 | 2013-07-11 | David Spetzler | Vesicle isolation methods |
| US8206929B2 (en) | 2009-01-07 | 2012-06-26 | Roche Molecular Systems, Inc. | Nucleic acid amplification with allele-specific suppression of sequence variants |
| KR101312241B1 (ko) | 2010-04-27 | 2013-09-27 | 사회복지법인 삼성생명공익재단 | 증폭억제시발체를 이용하는 유전자 돌연변이 검출 방법 |
| EP2646576B1 (en) | 2010-12-03 | 2015-10-28 | Brandeis University | Methods and kits for detecting nucleic acid mutants in wild-type populations |
| GB201100150D0 (en) | 2011-01-06 | 2011-02-23 | Epistem Ltd | Mutational analysis |
| EP2715347A4 (en) | 2011-05-26 | 2014-12-03 | Univ Brandeis | EVIDENCE OF SEQUENCE VARIANTS OF THE EPIDERMAL GROWTH FACTOR RECEPTOR (EGFR) GENE |
| US9133490B2 (en) | 2012-05-16 | 2015-09-15 | Transgenomic, Inc. | Step-up method for COLD-PCR enrichment |
-
2014
- 2014-10-20 JP JP2016524530A patent/JP2016535987A/ja not_active Withdrawn
- 2014-10-20 US US15/027,688 patent/US20160273022A1/en not_active Abandoned
- 2014-10-20 CN CN201480013111.4A patent/CN105283555A/zh active Pending
-
2016
- 2016-09-03 US US15/256,523 patent/US9725755B2/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020098510A1 (en) * | 2000-12-14 | 2002-07-25 | Affymetrix, Inc. | Blocker-aided target amplification of nucleic acids |
| CN101815789A (zh) * | 2007-08-01 | 2010-08-25 | 达纳-法伯癌症研究院有限公司 | 靶序列的富集 |
| CN102859003A (zh) * | 2010-03-08 | 2013-01-02 | 达纳-法伯癌症研究院有限公司 | 使用参考封闭序列的全cold-pcr富集 |
Non-Patent Citations (1)
| Title |
|---|
| PATRICK L DOMINGUEZ等: "Wild-type blocking polymerase chain reaction for detection of single nucleotide minority mutations from clinical specimens", 《ONCOGENE》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107012221A (zh) * | 2016-10-14 | 2017-08-04 | 苏州艾达康医疗科技有限公司 | 基于阻断物的富集系统及其应用 |
| CN106755451A (zh) * | 2017-01-05 | 2017-05-31 | 苏州艾达康医疗科技有限公司 | 核酸制备及分析 |
| CN108179191A (zh) * | 2018-02-05 | 2018-06-19 | 广州市达瑞生物技术股份有限公司 | 一种检测人类ctDNA基因突变的试剂盒 |
| CN108410982A (zh) * | 2018-02-05 | 2018-08-17 | 广州市达瑞生物技术股份有限公司 | 一种基于maldi-tof-ms检测肺癌34个突变位点的试剂盒 |
| CN109762901A (zh) * | 2019-01-30 | 2019-05-17 | 杭州瑞普基因科技有限公司 | 用于富集低频dna突变的dna探针应用于多种突变的同时检测 |
| CN111004839A (zh) * | 2019-12-18 | 2020-04-14 | 杭州瑞普基因科技有限公司 | 基于锁核酸修饰的阻滞物进行阻滞取代扩增富集检测目标突变的方法 |
| CN115287338A (zh) * | 2022-07-04 | 2022-11-04 | 苏州唯善生物科技有限公司 | 一种提高数字pcr灵敏度的检测方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| US20170009276A1 (en) | 2017-01-12 |
| JP2016535987A (ja) | 2016-11-24 |
| US9725755B2 (en) | 2017-08-08 |
| US20160273022A1 (en) | 2016-09-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105283555A (zh) | 核酸序列的合成和富集 | |
| US10329605B2 (en) | Method to increase sensitivity of detection of low-occurrence mutations | |
| US20160130641A1 (en) | Highly sensitive method for detecting low frequency mutations | |
| JP5818018B2 (ja) | 核酸増幅を用いる環状プライマー及びその応用 | |
| JP2017501683A (ja) | クランピングプローブ及び検出プローブを用いた多重標的核酸の検出方法 | |
| CN102796817B (zh) | 一种快速检测kras基因突变的方法 | |
| CA2828535A1 (en) | Kit and method for sequencing a target dna in a mixed population | |
| Oh et al. | Detection of low-level KRAS mutations using PNA-mediated asymmetric PCR clamping and melting curve analysis with unlabeled probes | |
| US20240287617A1 (en) | Kits and methods for detecting cancer-related mutations | |
| KR102294796B1 (ko) | 유전자 변이 검출법 | |
| US10351903B2 (en) | Allele amplification bias | |
| EP3350347B1 (en) | Methods and materials for detection of mutations | |
| Botezatu et al. | TaqMan probes as blocking agents for enriched PCR amplification and DNA melting analysis of mutant genes | |
| WO2015073163A2 (en) | Synthesis and enrichment of nucleic acid sequences | |
| CN105420349A (zh) | 确定突变核酸碱基的方法及试剂盒 | |
| CN105506116B (zh) | Braf基因检测物在制备acth型垂体腺瘤分子病理诊断及分型产品中的应用 | |
| KR20160106041A (ko) | Ｎｒａｓ 및 ｂｒａｆ 핵산의 멀티플렉스 분석을 위한 조성물 및 방법 | |
| WO2016093333A1 (ja) | 塩基変異の検出方法及びキット並びに核酸サンプルのｐｃｒ増幅を抑制する方法 | |
| US20140295431A1 (en) | Method of allele-specific amplification | |
| JP2025118633A (ja) | 高い特異度の標的核酸増幅方法及びこれを利用した標的核酸増幅用組成物 | |
| CN108004317A (zh) | Pik3ca基因突变检测体系及其试剂盒 | |
| JPWO2016103727A1 (ja) | 特異的核酸配列の増幅促進方法 | |
| EP2951321A2 (en) | Synthesis and enrichment of nucleic acid sequences | |
| US20150140564A1 (en) | Selective-competitive primer and method of use | |
| Botezatu et al. | Scanning for KRAS, NRAS, BRAF, and PIK3CA mutations by DNA melting analysis with TaqMan probes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160127 |