JP2016534731A5 - - Google Patents
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- Publication number
- JP2016534731A5 JP2016534731A5 JP2016527338A JP2016527338A JP2016534731A5 JP 2016534731 A5 JP2016534731 A5 JP 2016534731A5 JP 2016527338 A JP2016527338 A JP 2016527338A JP 2016527338 A JP2016527338 A JP 2016527338A JP 2016534731 A5 JP2016534731 A5 JP 2016534731A5
- Authority
- JP
- Japan
- Prior art keywords
- pluripotent stem
- stem cells
- cell population
- population
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 238000000034 method Methods 0.000 claims description 42
- 210000001778 pluripotent stem cell Anatomy 0.000 claims description 31
- 210000004027 cell Anatomy 0.000 claims description 25
- 210000000130 stem cell Anatomy 0.000 claims description 13
- 238000004114 suspension culture Methods 0.000 claims description 12
- 239000000725 suspension Substances 0.000 claims description 9
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 8
- 229940098773 bovine serum albumin Drugs 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 8
- 230000004069 differentiation Effects 0.000 claims description 7
- 102000040945 Transcription factor Human genes 0.000 claims description 4
- 108091023040 Transcription factor Proteins 0.000 claims description 4
- 210000005155 neural progenitor cell Anatomy 0.000 claims description 4
- 102100039939 Growth/differentiation factor 8 Human genes 0.000 claims description 3
- 206010021143 Hypoxia Diseases 0.000 claims description 3
- 210000004381 amniotic fluid Anatomy 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 210000001671 embryonic stem cell Anatomy 0.000 claims description 3
- 150000002632 lipids Chemical class 0.000 claims description 3
- 230000002107 myocardial effect Effects 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 210000003954 umbilical cord Anatomy 0.000 claims description 3
- 102100028096 Homeobox protein Nkx-6.2 Human genes 0.000 claims description 2
- 101000886562 Homo sapiens Growth/differentiation factor 8 Proteins 0.000 claims description 2
- 101000578254 Homo sapiens Homeobox protein Nkx-6.1 Proteins 0.000 claims description 2
- 101000578258 Homo sapiens Homeobox protein Nkx-6.2 Proteins 0.000 claims description 2
- 102100041030 Pancreas/duodenum homeobox protein 1 Human genes 0.000 claims description 2
- 101710183548 Pyridoxal 5'-phosphate synthase subunit PdxS Proteins 0.000 claims description 2
- 102000044880 Wnt3A Human genes 0.000 claims description 2
- 108700013515 Wnt3A Proteins 0.000 claims description 2
- 108010023082 activin A Proteins 0.000 claims description 2
- 210000000227 basophil cell of anterior lobe of hypophysis Anatomy 0.000 claims description 2
- 210000001900 endoderm Anatomy 0.000 claims description 2
- 230000001146 hypoxic effect Effects 0.000 claims description 2
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims description 2
- 239000003112 inhibitor Substances 0.000 claims description 2
- 101150068520 wnt3a gene Proteins 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims 6
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 2
- RHUJMHOIQBDFQR-UHFFFAOYSA-N 2-[[3-(2-methoxyphenyl)-4-oxo-6,7-dihydrothieno[3,2-d]pyrimidin-2-yl]sulfanyl]-n-(6-methyl-1,3-benzothiazol-2-yl)acetamide Chemical compound COC1=CC=CC=C1N1C(=O)C(SCC2)=C2N=C1SCC(=O)NC1=NC2=CC=C(C)C=C2S1 RHUJMHOIQBDFQR-UHFFFAOYSA-N 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 1
- 102000004877 Insulin Human genes 0.000 claims 1
- 108090001061 Insulin Proteins 0.000 claims 1
- 239000000853 adhesive Substances 0.000 claims 1
- 230000001070 adhesive effect Effects 0.000 claims 1
- 210000004413 cardiac myocyte Anatomy 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 239000008103 glucose Substances 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 230000007954 hypoxia Effects 0.000 claims 1
- 229940125396 insulin Drugs 0.000 claims 1
- 230000008186 parthenogenesis Effects 0.000 claims 1
- 239000003590 rho kinase inhibitor Substances 0.000 description 4
- 230000035519 G0 Phase Effects 0.000 description 2
- 230000010190 G1 phase Effects 0.000 description 2
- 102000043168 TGF-beta family Human genes 0.000 description 2
- 108091085018 TGF-beta family Proteins 0.000 description 2
- 230000022131 cell cycle Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000001776 parthenogenetic effect Effects 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 102100031650 C-X-C chemokine receptor type 4 Human genes 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 101000922348 Homo sapiens C-X-C chemokine receptor type 4 Proteins 0.000 description 1
- 108010056852 Myostatin Proteins 0.000 description 1
- 238000004115 adherent culture Methods 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361962158P | 2013-11-01 | 2013-11-01 | |
| US61/962,158 | 2013-11-01 | ||
| US13/998,974 US10377989B2 (en) | 2012-12-31 | 2013-12-30 | Methods for suspension cultures of human pluripotent stem cells |
| US13/998,974 | 2013-12-30 | ||
| PCT/US2014/038993 WO2015065524A2 (en) | 2013-11-01 | 2014-05-21 | Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2016534731A JP2016534731A (ja) | 2016-11-10 |
| JP2016534731A5 true JP2016534731A5 (cg-RX-API-DMAC7.html) | 2017-07-06 |
Family
ID=53004904
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016527338A Pending JP2016534731A (ja) | 2013-11-01 | 2014-05-21 | 膵内分泌細胞への分化のためのヒト多能性幹細胞の懸濁及び集団化 |
| JP2016527450A Pending JP2017500013A (ja) | 2013-11-01 | 2014-06-17 | 膵内分泌細胞への分化のためのヒト多能性幹細胞の懸濁及び集団化 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016527450A Pending JP2017500013A (ja) | 2013-11-01 | 2014-06-17 | 膵内分泌細胞への分化のためのヒト多能性幹細胞の懸濁及び集団化 |
Country Status (12)
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2016005657A (es) * | 2013-11-01 | 2017-01-05 | Janssen Biotech Inc | Suspensión y agrupamiento de células madre humanas pluripotentes para la diferenciación a células endocrinas pancreáticas. |
| RU2771328C2 (ru) * | 2016-06-23 | 2022-04-29 | Титон Биотек, Инк. | Клетки, экспрессирующие рецептор паратиреоидного гормона 1, и их применение |
| WO2018089011A1 (en) | 2016-11-10 | 2018-05-17 | Viacyte, Inc | Pdx1 pancreatic endoderm cells in cell delivery devices and methods thereof |
| JP7024978B2 (ja) | 2016-11-16 | 2022-03-03 | サイナータ セラピューティクス リミテッド | 多能性幹細胞のアッセイ |
| US10391156B2 (en) | 2017-07-12 | 2019-08-27 | Viacyte, Inc. | University donor cells and related methods |
| WO2019018818A1 (en) * | 2017-07-21 | 2019-01-24 | Semma Therapeutics, Inc. | REAGGREGATION OF BETA PANCREATIC CELLS DERIVED FROM STEM CELLS |
| JP7000896B2 (ja) * | 2017-08-23 | 2022-01-19 | 王子ホールディングス株式会社 | 細胞シート形成部材、細胞シート形成部材の製造方法、および、細胞シートの製造方法 |
| EP3735457A4 (en) * | 2018-01-05 | 2021-08-25 | Platelet Biogenesis, Inc. | COMPOSITIONS AND METHOD OF MANUFACTURING MEGAKARYOCYTE |
| WO2019143292A1 (en) | 2018-01-18 | 2019-07-25 | Agency For Science, Technology And Research | Method for differentiation of human pluripotent stem cell lines in suspension culture |
| KR102115360B1 (ko) * | 2018-05-30 | 2020-05-26 | 주식회사 바이블리오테카 | 성체 줄기세포 배양액 및 그 제조방법 |
| US12378572B2 (en) | 2018-09-07 | 2025-08-05 | Crispr Therapeutics Ag | Universal donor cells |
| US20220233298A1 (en) | 2019-05-31 | 2022-07-28 | W. L. Gore & Associates, Inc. | A biocompatible membrane composite |
| CN114401752B (zh) | 2019-05-31 | 2023-04-04 | W.L.戈尔及同仁股份有限公司 | 具有受控氧扩散距离的细胞封装装置 |
| WO2020243663A1 (en) | 2019-05-31 | 2020-12-03 | W. L. Gore & Associates, Inc. | A biocompatible membrane composite |
| AU2020283056B2 (en) | 2019-05-31 | 2023-06-08 | Viacyte, Inc. | A biocompatible membrane composite |
| WO2021041555A1 (en) * | 2019-08-27 | 2021-03-04 | United Therapeutics Corporation | Methods and compositions for culturing alveolar cells |
| KR20220052370A (ko) | 2019-09-05 | 2022-04-27 | 크리스퍼 테라퓨틱스 아게 | 보편적 공여자 세포 |
| US11116798B2 (en) | 2019-09-05 | 2021-09-14 | Crispr Therapeutics Ag | Universal donor cells |
| CN114729323B (zh) * | 2019-11-22 | 2024-09-17 | 诺和诺德股份有限公司 | 旋转聚集的神经微球及其应用 |
| AU2021414617A1 (en) | 2020-12-31 | 2023-08-10 | Crispr Therapeutics Ag | Universal donor cells |
| JPWO2022203051A1 (cg-RX-API-DMAC7.html) * | 2021-03-25 | 2022-09-29 | ||
| CN116218766A (zh) * | 2023-04-21 | 2023-06-06 | 北京意胜生物科技有限公司 | 一种制备胰腺前体细胞的方法、培养基及其应用 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR0213805A (pt) * | 2001-11-09 | 2005-08-16 | Artecel Sciences Inc | Células estromais derivadas de tecido adiposo para diferenciação endócrina do pâncreas e usos das mesmas |
| EP2733203B1 (en) * | 2006-08-02 | 2018-10-10 | Technion Research & Development Foundation Ltd. | Methods of expanding embryonic stem cells in a suspension culture |
| EP2088190A4 (en) * | 2006-11-09 | 2011-01-05 | Japan Government | METHOD FOR THE CULTURE AND PASSING OF AN EMBRYONAL PRIMATIVE STEM CELL AND METHOD FOR INDUCING THE DIFFERENTIATION OF THE EMBRYONAL STEM CELL |
| WO2008148105A1 (en) * | 2007-05-25 | 2008-12-04 | Medistem Laboratories, Inc. | Endometrial stem cells and methods of making and using same |
| JP2010532172A (ja) * | 2007-07-01 | 2010-10-07 | ライフスキャン・インコーポレイテッド | 単細胞の分化万能性幹細胞の培養 |
| EP2271747B1 (en) * | 2008-03-17 | 2016-09-07 | Agency for Science, Technology And Research | Microcarriers for pluripotent stem cell culture |
| KR20180018839A (ko) * | 2008-06-30 | 2018-02-21 | 얀센 바이오테크 인코포레이티드 | 만능 줄기 세포의 분화 |
| US8008075B2 (en) * | 2008-11-04 | 2011-08-30 | Viacyte, Inc. | Stem cell aggregate suspension compositions and methods of differentiation thereof |
| JP5761816B2 (ja) * | 2009-08-12 | 2015-08-12 | 国立大学法人京都大学 | 多能性幹細胞から神経前駆細胞への分化誘導法 |
| US8932857B2 (en) * | 2010-06-15 | 2015-01-13 | Kyoto University | Method for selecting reduced differentiation resistance human induced pluripotent stem cells |
| ES2659393T3 (es) * | 2010-08-31 | 2018-03-15 | Janssen Biotech, Inc. | Diferenciación de células madre embrionarias humanas |
| WO2012117333A1 (en) * | 2011-02-28 | 2012-09-07 | Stempeutics Research Malaysia Sdn Bhd | Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof |
| US8951798B2 (en) * | 2011-10-13 | 2015-02-10 | Wisconsin Alumni Research Foundation | Generation of cardiomyocytes from human pluripotent stem cells |
| SG11201403473QA (en) * | 2011-12-22 | 2014-10-30 | Janssen Biotech Inc | Differentiation of human embryonic stem cells into single hormonal insulin positive cells |
| US10519422B2 (en) * | 2012-02-29 | 2019-12-31 | Riken | Method of producing human retinal pigment epithelial cells |
| CA2896750A1 (en) * | 2012-12-31 | 2014-07-03 | Janssen Biotech, Inc. | Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells |
| MX2016005657A (es) * | 2013-11-01 | 2017-01-05 | Janssen Biotech Inc | Suspensión y agrupamiento de células madre humanas pluripotentes para la diferenciación a células endocrinas pancreáticas. |
-
2014
- 2014-05-21 MX MX2016005657A patent/MX2016005657A/es unknown
- 2014-05-21 SG SG11201603045VA patent/SG11201603045VA/en unknown
- 2014-05-21 CN CN201480059910.5A patent/CN105793413A/zh active Pending
- 2014-05-21 WO PCT/US2014/038993 patent/WO2015065524A2/en not_active Ceased
- 2014-05-21 AU AU2014342995A patent/AU2014342995C1/en not_active Ceased
- 2014-05-21 JP JP2016527338A patent/JP2016534731A/ja active Pending
- 2014-05-21 KR KR1020187034291A patent/KR20180128529A/ko not_active Abandoned
- 2014-05-21 EP EP14857034.4A patent/EP3063268A4/en not_active Withdrawn
- 2014-05-21 CA CA2928639A patent/CA2928639A1/en not_active Abandoned
- 2014-05-21 KR KR1020167014409A patent/KR20160079072A/ko not_active Ceased
- 2014-05-21 RU RU2016121409A patent/RU2689710C2/ru active
- 2014-06-17 BR BR112016009393A patent/BR112016009393A8/pt not_active Application Discontinuation
- 2014-06-17 CN CN201480059902.0A patent/CN105683362A/zh active Pending
- 2014-06-17 CA CA2928741A patent/CA2928741A1/en not_active Abandoned
- 2014-06-17 MX MX2016005658A patent/MX2016005658A/es unknown
- 2014-06-17 KR KR1020187034483A patent/KR20180130001A/ko not_active Withdrawn
- 2014-06-17 JP JP2016527450A patent/JP2017500013A/ja active Pending
- 2014-06-17 AU AU2014343007A patent/AU2014343007C1/en not_active Ceased
- 2014-06-17 SG SG11201603047PA patent/SG11201603047PA/en unknown
- 2014-06-17 RU RU2016121404A patent/RU2016121404A/ru not_active Application Discontinuation
- 2014-06-17 EP EP14859059.9A patent/EP3063269A4/en not_active Withdrawn
- 2014-06-17 KR KR1020167014406A patent/KR20160079071A/ko not_active Abandoned
- 2014-06-17 WO PCT/US2014/042796 patent/WO2015065537A1/en not_active Ceased
-
2016
- 2016-04-27 PH PH12016500783A patent/PH12016500783A1/en unknown
- 2016-04-27 PH PH12016500782A patent/PH12016500782A1/en unknown
-
2018
- 2018-07-26 AU AU2018208707A patent/AU2018208707A1/en not_active Abandoned
- 2018-07-26 AU AU2018208717A patent/AU2018208717A1/en not_active Abandoned
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