JP2016524917A - 複合的な選択性を有する特異的核酸増幅 - Google Patents

複合的な選択性を有する特異的核酸増幅 Download PDF

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Publication number
JP2016524917A
JP2016524917A JP2016527125A JP2016527125A JP2016524917A JP 2016524917 A JP2016524917 A JP 2016524917A JP 2016527125 A JP2016527125 A JP 2016527125A JP 2016527125 A JP2016527125 A JP 2016527125A JP 2016524917 A JP2016524917 A JP 2016524917A
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nucleic acid
domain
target
primer
strand
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Japanese (ja)
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JP2016524917A5 (enExample
Inventor
チェン，シ
ジャン，デーヴィッド，ユ
イン，ペン
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Harvard University
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Harvard University
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Publication of JP2016524917A publication Critical patent/JP2016524917A/ja
Publication of JP2016524917A5 publication Critical patent/JP2016524917A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2016527125A 2013-07-18 2014-07-18 複合的な選択性を有する特異的核酸増幅 Pending JP2016524917A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361856028P 2013-07-18 2013-07-18
US61/856,028 2013-07-18
PCT/US2014/047196 WO2015010020A1 (en) 2013-07-18 2014-07-18 Specific nucleic acid amplification with compounded selectivity

Publications (2)

Publication Number Publication Date
JP2016524917A true JP2016524917A (ja) 2016-08-22
JP2016524917A5 JP2016524917A5 (enExample) 2017-08-31

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JP2016527125A Pending JP2016524917A (ja) 2013-07-18 2014-07-18 複合的な選択性を有する特異的核酸増幅

Country Status (7)

Country Link
US (1) US10745746B2 (enExample)
EP (1) EP3022323B1 (enExample)
JP (1) JP2016524917A (enExample)
CN (1) CN105492629A (enExample)
AU (1) AU2014290454A1 (enExample)
CA (1) CA2917627A1 (enExample)
WO (1) WO2015010020A1 (enExample)

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CN103328654B (zh) 2010-10-27 2017-07-11 哈佛学院院长等 立足点引物双链体的组合物及其使用方法
US9796748B2 (en) 2011-05-02 2017-10-24 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
US9862994B2 (en) 2012-02-09 2018-01-09 Dana-Farber Cancer Institute, Inc. Selective nucleic acid amplification from nucleic acid pools
WO2015010020A1 (en) 2013-07-18 2015-01-22 President And Fellows Of Harvard College Specific nucleic acid amplification with compounded selectivity
WO2015088990A1 (en) 2013-12-09 2015-06-18 Durect Corporation Pharmaceutically active agent complexes, polymer complexes, and compositions and methods involving the same
WO2016161237A1 (en) * 2015-04-03 2016-10-06 President And Fellows Of Harvard College Nucleic acid retro-activated primers
WO2017147585A1 (en) 2016-02-26 2017-08-31 Arizona Board Of Regents On Behalf Of Arizona State University Synthetic translation-sensing riboswitches and uses thereof
WO2017193025A1 (en) * 2016-05-06 2017-11-09 William Marsh Rice University Stoichiometric nucleic acid purification using randomer capture probe libraries
EP4108781A1 (en) 2016-05-27 2022-12-28 President and Fellows of Harvard College Conditional primer extension for single-molecule detection
US11773430B2 (en) 2016-10-17 2023-10-03 Arizona Board Of Regents On Behalf Of Arizona State University Unimolecular aptamer-based sensors for pathogen detection
WO2018093898A1 (en) * 2016-11-15 2018-05-24 Arizona Board Of Regents On Behalf Of Arizona State University Ultraspecific nucleic acid sensors for low-cost liquid biopsies
US12054711B2 (en) 2017-07-14 2024-08-06 City Of Hope Meta-stable oligonucleotides junctions for delivery of therapeutics
CN111630166B (zh) 2017-08-10 2024-04-19 希望之城 条件性-siRNA及其在治疗心肌肥大中的用途
WO2019033083A1 (en) 2017-08-10 2019-02-14 City Of Hope CONDITIONAL RNAIDS AND THEIR USES IN THE TREATMENT OF ACUTE MYELOID LEUKEMIA
JP7566741B2 (ja) 2018-08-10 2024-10-15 シティ・オブ・ホープ プログラム可能なｓｉＲＮＡ及びその使用
US11104941B2 (en) * 2018-09-28 2021-08-31 Bioo Scientific Corporation 5′ adapter comprising an internal 5′-5′ linkage
US20210388431A1 (en) * 2018-10-29 2021-12-16 Igor V. Kutyavin Thermokinetically balanced isothermal amplification of nucleic acid sequences
CA3176545A1 (en) * 2020-04-22 2021-10-28 President And Fellows Of Harvard College Isothermal methods, compositions, kits, and systems for detecting nucleic acids
CN118302819A (zh) * 2021-07-06 2024-07-05 斯威齐治疗公司 产生条件可激活核酸复合物中的核心链的方法
EP4490316A4 (en) * 2022-03-11 2026-03-04 Harvard College Multiplex Fluorescent Cell and Tissue Imaging with DNA-Encoded Thermal Channels and Their Uses
CN115125292B (zh) * 2022-06-02 2025-02-07 武汉大学 一种内切酶辅助的rna中肌苷修饰的单碱基分辨率定位分析方法

Citations (3)

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JP2010519896A (ja) * 2007-03-01 2010-06-10 オキシテック・リミテッド 核酸配列の検出方法
WO2012058488A1 (en) * 2010-10-27 2012-05-03 President And Fellows Of Harvard College Compositions of toehold primer duplexes and methods of use
WO2012149154A1 (en) * 2011-04-26 2012-11-01 Swift Biosciences, Inc. Polynucleotide primers and probes

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US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
CA2418724A1 (en) 2000-09-13 2002-03-21 Archemix Corporation Target activated nucleic acid biosensor and methods of using same
WO2003095664A2 (en) 2002-05-10 2003-11-20 City Of Hope Pyrophosphorolysis activated polymerization (pap)
US20040086892A1 (en) 2002-11-06 2004-05-06 Crothers Donald M. Universal tag assay
EP1799825B1 (en) 2004-10-05 2011-06-29 The California Institute of Technology Aptamer regulated nucleic acids and uses thereof
US20100009355A1 (en) 2006-03-14 2010-01-14 Kolodney Michael S Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
GB0703996D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Nucleic acid detection
WO2010120853A2 (en) 2009-04-16 2010-10-21 Padma Arunachalam Methods and compositions to detect and differentiate small rnas in rna maturation pathway
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US9796748B2 (en) 2011-05-02 2017-10-24 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
US9862994B2 (en) 2012-02-09 2018-01-09 Dana-Farber Cancer Institute, Inc. Selective nucleic acid amplification from nucleic acid pools
WO2014014988A2 (en) 2012-07-17 2014-01-23 Dna Logix, Inc. Cooperative primers, probes, and applications thereof
WO2015010020A1 (en) 2013-07-18 2015-01-22 President And Fellows Of Harvard College Specific nucleic acid amplification with compounded selectivity
WO2016161237A1 (en) 2015-04-03 2016-10-06 President And Fellows Of Harvard College Nucleic acid retro-activated primers
EP4108781A1 (en) 2016-05-27 2022-12-28 President and Fellows of Harvard College Conditional primer extension for single-molecule detection

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
JP2010519896A (ja) * 2007-03-01 2010-06-10 オキシテック・リミテッド 核酸配列の検出方法
WO2012058488A1 (en) * 2010-10-27 2012-05-03 President And Fellows Of Harvard College Compositions of toehold primer duplexes and methods of use
WO2012149154A1 (en) * 2011-04-26 2012-11-01 Swift Biosciences, Inc. Polynucleotide primers and probes

Non-Patent Citations (1)

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Also Published As

Publication number Publication date
EP3022323A1 (en) 2016-05-25
EP3022323B1 (en) 2019-04-24
WO2015010020A1 (en) 2015-01-22
US20160153036A1 (en) 2016-06-02
CA2917627A1 (en) 2015-01-22
US10745746B2 (en) 2020-08-18
CN105492629A (zh) 2016-04-13
EP3022323A4 (en) 2017-05-03
AU2014290454A1 (en) 2016-01-28

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