JP2016524917A - 複合的な選択性を有する特異的核酸増幅 - Google Patents

複合的な選択性を有する特異的核酸増幅 Download PDF

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JP2016524917A
JP2016524917A JP2016527125A JP2016527125A JP2016524917A JP 2016524917 A JP2016524917 A JP 2016524917A JP 2016527125 A JP2016527125 A JP 2016527125A JP 2016527125 A JP2016527125 A JP 2016527125A JP 2016524917 A JP2016524917 A JP 2016524917A
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nucleic acid
domain
target
primer
strand
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Japanese (ja)
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JP2016524917A5 (enExample
Inventor
チェン，シ
ジャン，デーヴィッド，ユ
イン，ペン
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Harvard University
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Harvard University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2016527125A 2013-07-18 2014-07-18 複合的な選択性を有する特異的核酸増幅 Pending JP2016524917A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361856028P 2013-07-18 2013-07-18
US61/856,028 2013-07-18
PCT/US2014/047196 WO2015010020A1 (en) 2013-07-18 2014-07-18 Specific nucleic acid amplification with compounded selectivity

Publications (2)

Publication Number Publication Date
JP2016524917A true JP2016524917A (ja) 2016-08-22
JP2016524917A5 JP2016524917A5 (enExample) 2017-08-31

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JP2016527125A Pending JP2016524917A (ja) 2013-07-18 2014-07-18 複合的な選択性を有する特異的核酸増幅

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Country Link
US (1) US10745746B2 (enExample)
EP (1) EP3022323B1 (enExample)
JP (1) JP2016524917A (enExample)
CN (1) CN105492629A (enExample)
AU (1) AU2014290454A1 (enExample)
CA (1) CA2917627A1 (enExample)
WO (1) WO2015010020A1 (enExample)

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WO2012151328A2 (en) 2011-05-02 2012-11-08 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
US9862994B2 (en) 2012-02-09 2018-01-09 Dana-Farber Cancer Institute, Inc. Selective nucleic acid amplification from nucleic acid pools
EP3022323B1 (en) 2013-07-18 2019-04-24 President and Fellows of Harvard College Specific nucleic acid amplification with compounded selectivity
US20160303242A1 (en) 2013-12-09 2016-10-20 Durect Corporation Pharmaceutically Active Agent Complexes, Polymer Complexes, and Compositions and Methods Involving the Same
WO2016161237A1 (en) * 2015-04-03 2016-10-06 President And Fellows Of Harvard College Nucleic acid retro-activated primers
US10550440B2 (en) 2016-02-26 2020-02-04 Arizona Board Of Regents On Behalf Of Arizona State University Synthetic translation-sensing riboswitches and uses thereof
CN109563506A (zh) * 2016-05-06 2019-04-02 威廉马歇莱思大学 使用随机捕获探针文库的化学计量核酸纯化
WO2017205719A1 (en) 2016-05-27 2017-11-30 President And Fellows Of Harvard College Conditional primer extension for single-molecule detection
US11773430B2 (en) 2016-10-17 2023-10-03 Arizona Board Of Regents On Behalf Of Arizona State University Unimolecular aptamer-based sensors for pathogen detection
WO2018093898A1 (en) * 2016-11-15 2018-05-24 Arizona Board Of Regents On Behalf Of Arizona State University Ultraspecific nucleic acid sensors for low-cost liquid biopsies
WO2019014656A1 (en) 2017-07-14 2019-01-17 Han Si Ping METALLIC OLIGONUCLEOTIDE JONCTIONS FOR THE ADMINISTRATION OF THERAPEUTIC AGENTS
WO2019033079A1 (en) 2017-08-10 2019-02-14 City Of Hope CONDITIONAL RNAIDS AND THEIR USES IN THE TREATMENT OF CARDIAC HYPERTROPHY
EP3833757A4 (en) 2018-08-10 2022-05-18 City of Hope Programmable conditional sirnas and uses thereof
US11104941B2 (en) * 2018-09-28 2021-08-31 Bioo Scientific Corporation 5′ adapter comprising an internal 5′-5′ linkage
WO2020092268A2 (en) * 2018-10-29 2020-05-07 Kutyavin Igor V Thermokinetically balanced isothermal amplification of nucleic acid sequences
CA3176545A1 (en) * 2020-04-22 2021-10-28 President And Fellows Of Harvard College Isothermal methods, compositions, kits, and systems for detecting nucleic acids
EP4367673A4 (en) * 2021-07-06 2025-05-14 Switch Therapeutics Inc. METHOD FOR GENERATING CORE STRANDS IN CONDITIONALLY ACTIVABLE NUCLEIC ACID COMPLEXES
CN115125292B (zh) * 2022-06-02 2025-02-07 武汉大学 一种内切酶辅助的rna中肌苷修饰的单碱基分辨率定位分析方法

Citations (3)

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JP2010519896A (ja) * 2007-03-01 2010-06-10 オキシテック・リミテッド 核酸配列の検出方法
WO2012058488A1 (en) * 2010-10-27 2012-05-03 President And Fellows Of Harvard College Compositions of toehold primer duplexes and methods of use
WO2012149154A1 (en) * 2011-04-26 2012-11-01 Swift Biosciences, Inc. Polynucleotide primers and probes

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US20040086892A1 (en) 2002-11-06 2004-05-06 Crothers Donald M. Universal tag assay
JP5101288B2 (ja) 2004-10-05 2012-12-19 カリフォルニア インスティテュート オブ テクノロジー アプタマー調節される核酸及びその利用
WO2007106534A2 (en) 2006-03-14 2007-09-20 Harbor-Ucla Research And Education Institute Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
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US9862994B2 (en) 2012-02-09 2018-01-09 Dana-Farber Cancer Institute, Inc. Selective nucleic acid amplification from nucleic acid pools
KR102270892B1 (ko) 2012-07-17 2021-06-30 디엔에이 로직스, 인크. 공조 프라이머, 프로브 그리고 이의 용도
EP3022323B1 (en) 2013-07-18 2019-04-24 President and Fellows of Harvard College Specific nucleic acid amplification with compounded selectivity
WO2016161237A1 (en) 2015-04-03 2016-10-06 President And Fellows Of Harvard College Nucleic acid retro-activated primers
WO2017205719A1 (en) 2016-05-27 2017-11-30 President And Fellows Of Harvard College Conditional primer extension for single-molecule detection

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
JP2010519896A (ja) * 2007-03-01 2010-06-10 オキシテック・リミテッド 核酸配列の検出方法
WO2012058488A1 (en) * 2010-10-27 2012-05-03 President And Fellows Of Harvard College Compositions of toehold primer duplexes and methods of use
WO2012149154A1 (en) * 2011-04-26 2012-11-01 Swift Biosciences, Inc. Polynucleotide primers and probes

Non-Patent Citations (1)

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Title
J AM CHEM SOC, vol. 133, no. 7, JPN6018025484, 2011, pages 2177 - 2182, ISSN: 0003832124 *

Also Published As

Publication number Publication date
US20160153036A1 (en) 2016-06-02
EP3022323A1 (en) 2016-05-25
US10745746B2 (en) 2020-08-18
WO2015010020A1 (en) 2015-01-22
EP3022323A4 (en) 2017-05-03
AU2014290454A1 (en) 2016-01-28
EP3022323B1 (en) 2019-04-24
CN105492629A (zh) 2016-04-13
CA2917627A1 (en) 2015-01-22

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