CN105492629A - 具有复合选择性的特异性核酸扩增 - Google Patents

具有复合选择性的特异性核酸扩增 Download PDF

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Publication number
CN105492629A
CN105492629A CN201480045718.0A CN201480045718A CN105492629A CN 105492629 A CN105492629 A CN 105492629A CN 201480045718 A CN201480045718 A CN 201480045718A CN 105492629 A CN105492629 A CN 105492629A
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nucleic acid
target
strand
pseudo
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Chinese (zh)
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X·陈
D·Y·张
尹鹏
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Harvard University
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Harvard University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201480045718.0A 2013-07-18 2014-07-18 具有复合选择性的特异性核酸扩增 Pending CN105492629A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361856028P 2013-07-18 2013-07-18
US61/856,028 2013-07-18
PCT/US2014/047196 WO2015010020A1 (en) 2013-07-18 2014-07-18 Specific nucleic acid amplification with compounded selectivity

Publications (1)

Publication Number Publication Date
CN105492629A true CN105492629A (zh) 2016-04-13

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CN201480045718.0A Pending CN105492629A (zh) 2013-07-18 2014-07-18 具有复合选择性的特异性核酸扩增

Country Status (7)

Country Link
US (1) US10745746B2 (enExample)
EP (1) EP3022323B1 (enExample)
JP (1) JP2016524917A (enExample)
CN (1) CN105492629A (enExample)
AU (1) AU2014290454A1 (enExample)
CA (1) CA2917627A1 (enExample)
WO (1) WO2015010020A1 (enExample)

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CN109563506A (zh) * 2016-05-06 2019-04-02 威廉马歇莱思大学 使用随机捕获探针文库的化学计量核酸纯化
CN112996921A (zh) * 2018-09-28 2021-06-18 Bioo科技公司 包含内部5’-5’连接的5’衔接子
CN115125292A (zh) * 2022-06-02 2022-09-30 武汉大学 一种内切酶辅助的rna中肌苷修饰的单碱基分辨率定位分析方法

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JP5960709B2 (ja) 2010-10-27 2016-08-02 プレジデント アンド フェローズ オブ ハーバード カレッジ トーホールドプライマーデュプレックスの組成物およびその使用方法
WO2012151328A2 (en) 2011-05-02 2012-11-08 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
US9862994B2 (en) 2012-02-09 2018-01-09 Dana-Farber Cancer Institute, Inc. Selective nucleic acid amplification from nucleic acid pools
EP3022323B1 (en) 2013-07-18 2019-04-24 President and Fellows of Harvard College Specific nucleic acid amplification with compounded selectivity
EP3079668A1 (en) 2013-12-09 2016-10-19 Durect Corporation Pharmaceutically active agent complexes, polymer complexes, and compositions and methods involving the same
US20180094309A1 (en) * 2015-04-03 2018-04-05 President And Fellows Of Harvard College Nucleic acid retro-activated primers
WO2017147585A1 (en) 2016-02-26 2017-08-31 Arizona Board Of Regents On Behalf Of Arizona State University Synthetic translation-sensing riboswitches and uses thereof
EP3464636B1 (en) 2016-05-27 2022-07-06 President and Fellows of Harvard College Conditional primer extension for single-molecule detection
US11773430B2 (en) 2016-10-17 2023-10-03 Arizona Board Of Regents On Behalf Of Arizona State University Unimolecular aptamer-based sensors for pathogen detection
US11434540B2 (en) 2016-11-15 2022-09-06 Arizona Board Of Regents On Behalf Of Arizona State University Ultraspecific nucleic acid sensors for low-cost liquid biopsies
WO2019014656A1 (en) 2017-07-14 2019-01-17 Han Si Ping METALLIC OLIGONUCLEOTIDE JONCTIONS FOR THE ADMINISTRATION OF THERAPEUTIC AGENTS
CN111630166B (zh) 2017-08-10 2024-04-19 希望之城 条件性-siRNA及其在治疗心肌肥大中的用途
CN113166750B (zh) 2018-08-10 2025-02-18 希望之城 可编程的条件性sirna及其用途
WO2020092255A1 (en) * 2018-10-29 2020-05-07 Kutyavin Igor V Accelerated polymerase chain reaction
AU2021261343A1 (en) * 2020-04-22 2022-11-03 President And Fellows Of Harvard College Isothermal methods, compositions, kits, and systems for detecting nucleic acids
EP4367673A4 (en) * 2021-07-06 2025-05-14 Switch Therapeutics Inc. METHOD FOR GENERATING CORE STRANDS IN CONDITIONALLY ACTIVABLE NUCLEIC ACID COMPLEXES
CN119173639A (zh) * 2022-03-11 2024-12-20 哈佛大学校长及研究员协会 用dna编码的热通道进行的多重荧光细胞和组织成像及其用途

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AU2001290965A1 (en) 2000-09-13 2002-03-26 Archemix Corporation Target activated nucleic acid biosensor and methods of using same
US20040086892A1 (en) 2002-11-06 2004-05-06 Crothers Donald M. Universal tag assay
ATE514776T1 (de) 2004-10-05 2011-07-15 California Inst Of Techn Aptamer-regulierte nukleinsäuren und verwendungen davon
US20100009355A1 (en) 2006-03-14 2010-01-14 Kolodney Michael S Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
GB0703997D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Methods for detecting nucleic sequences
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US20180094309A1 (en) 2015-04-03 2018-04-05 President And Fellows Of Harvard College Nucleic acid retro-activated primers
EP3464636B1 (en) 2016-05-27 2022-07-06 President and Fellows of Harvard College Conditional primer extension for single-molecule detection

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109563506A (zh) * 2016-05-06 2019-04-02 威廉马歇莱思大学 使用随机捕获探针文库的化学计量核酸纯化
CN112996921A (zh) * 2018-09-28 2021-06-18 Bioo科技公司 包含内部5’-5’连接的5’衔接子
CN112996921B (zh) * 2018-09-28 2024-05-03 Bioo科技公司 包含内部5’-5’连接的5’衔接子
CN115125292A (zh) * 2022-06-02 2022-09-30 武汉大学 一种内切酶辅助的rna中肌苷修饰的单碱基分辨率定位分析方法
CN115125292B (zh) * 2022-06-02 2025-02-07 武汉大学 一种内切酶辅助的rna中肌苷修饰的单碱基分辨率定位分析方法

Also Published As

Publication number Publication date
US20160153036A1 (en) 2016-06-02
AU2014290454A1 (en) 2016-01-28
EP3022323B1 (en) 2019-04-24
US10745746B2 (en) 2020-08-18
EP3022323A1 (en) 2016-05-25
EP3022323A4 (en) 2017-05-03
JP2016524917A (ja) 2016-08-22
CA2917627A1 (en) 2015-01-22
WO2015010020A1 (en) 2015-01-22

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