JP2016523560A5 - - Google Patents
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- JP2016523560A5 JP2016523560A5 JP2016525414A JP2016525414A JP2016523560A5 JP 2016523560 A5 JP2016523560 A5 JP 2016523560A5 JP 2016525414 A JP2016525414 A JP 2016525414A JP 2016525414 A JP2016525414 A JP 2016525414A JP 2016523560 A5 JP2016523560 A5 JP 2016523560A5
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- Prior art keywords
- rna
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- target nucleic
- nucleic acid
- protein
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- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 47
- 210000004027 cell Anatomy 0.000 claims description 44
- 108091033409 CRISPR Proteins 0.000 claims description 31
- 101710163270 Nuclease Proteins 0.000 claims description 31
- 238000000034 method Methods 0.000 claims description 26
- 230000002950 deficient Effects 0.000 claims description 22
- 108090000623 proteins and genes Proteins 0.000 claims description 21
- 102000004169 proteins and genes Human genes 0.000 claims description 19
- 108020005004 Guide RNA Proteins 0.000 claims description 12
- 230000000295 complement effect Effects 0.000 claims description 12
- 108020004414 DNA Proteins 0.000 claims description 6
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims description 5
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims description 5
- 230000027455 binding Effects 0.000 claims description 5
- 230000004927 fusion Effects 0.000 claims description 5
- 238000013518 transcription Methods 0.000 claims description 5
- 230000035897 transcription Effects 0.000 claims description 5
- 238000010354 CRISPR gene editing Methods 0.000 claims description 4
- 108020004707 nucleic acids Proteins 0.000 claims 61
- 102000039446 nucleic acids Human genes 0.000 claims 61
- 150000007523 nucleic acids Chemical class 0.000 claims 60
- 102000052510 DNA-Binding Proteins Human genes 0.000 claims 21
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 claims 18
- 101710096438 DNA-binding protein Proteins 0.000 claims 17
- 101000709520 Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B) Atypical response regulator protein ChxR Proteins 0.000 claims 11
- 239000002773 nucleotide Substances 0.000 claims 6
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- 230000002411 adverse Effects 0.000 claims 5
- 201000010099 disease Diseases 0.000 claims 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 5
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- 108700020911 DNA-Binding Proteins Proteins 0.000 claims 4
- 230000004570 RNA-binding Effects 0.000 claims 4
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- 241001525611 Corynebacterium kroppenstedtii DSM 44385 Species 0.000 description 1
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- 241000382842 Flavobacterium psychrophilum Species 0.000 description 1
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- 101000976622 Homo sapiens Zinc finger protein 42 homolog Proteins 0.000 description 1
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- 241001459443 Rhodococcus jostii RHA1 Species 0.000 description 1
- 241001113889 Rhodococcus opacus B4 Species 0.000 description 1
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- 241000933177 Shewanella pealeana ATCC 700345 Species 0.000 description 1
- 241001496704 Slackia heliotrinireducens DSM 20476 Species 0.000 description 1
- 241001478878 Streptobacillus Species 0.000 description 1
- 241001288016 Streptococcus gallolyticus Species 0.000 description 1
- 241001147754 Streptococcus gordonii str. Challis Species 0.000 description 1
- 241000672607 Streptococcus mutans NN2025 Species 0.000 description 1
- 241000103155 Streptococcus pyogenes MGAS10270 Species 0.000 description 1
- 241001520169 Streptococcus pyogenes MGAS315 Species 0.000 description 1
- 241001332083 Streptococcus pyogenes MGAS6180 Species 0.000 description 1
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- 241001496716 Streptococcus pyogenes NZ131 Species 0.000 description 1
- 241001217892 Streptococcus thermophilus CNRZ1066 Species 0.000 description 1
- 241001633172 Streptococcus thermophilus LMD-9 Species 0.000 description 1
- 241000079831 Streptococcus thermophilus LMG 18311 Species 0.000 description 1
- 241000192593 Synechocystis sp. PCC 6803 Species 0.000 description 1
- 241001496699 Thermomonospora curvata DSM 43183 Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361844844P | 2013-07-10 | 2013-07-10 | |
| US61/844,844 | 2013-07-10 | ||
| PCT/US2014/045700 WO2015006294A2 (en) | 2013-07-10 | 2014-07-08 | Orthogonal cas9 proteins for rna-guided gene regulation and editing |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019238875A Division JP7153992B2 (ja) | 2013-07-10 | 2019-12-27 | Ｒｎａ誘導性遺伝子制御および編集のための直交性ｃａｓ９タンパク質 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016523560A JP2016523560A (ja) | 2016-08-12 |
| JP2016523560A5 true JP2016523560A5 (https=) | 2017-07-27 |
| JP6718813B2 JP6718813B2 (ja) | 2020-07-08 |
Family
ID=52280701
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016525414A Active JP6718813B2 (ja) | 2013-07-10 | 2014-07-08 | Ｒｎａ誘導性遺伝子制御および編集のための直交性ｃａｓ９タンパク質 |
| JP2019238875A Active JP7153992B2 (ja) | 2013-07-10 | 2019-12-27 | Ｒｎａ誘導性遺伝子制御および編集のための直交性ｃａｓ９タンパク質 |
| JP2022159833A Pending JP2022176275A (ja) | 2013-07-10 | 2022-10-03 | Ｒｎａ誘導性遺伝子制御および編集のための直交性ｃａｓ９タンパク質 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019238875A Active JP7153992B2 (ja) | 2013-07-10 | 2019-12-27 | Ｒｎａ誘導性遺伝子制御および編集のための直交性ｃａｓ９タンパク質 |
| JP2022159833A Pending JP2022176275A (ja) | 2013-07-10 | 2022-10-03 | Ｒｎａ誘導性遺伝子制御および編集のための直交性ｃａｓ９タンパク質 |
Country Status (12)
| Country | Link |
|---|---|
| US (4) | US10329587B2 (https=) |
| EP (2) | EP3019204B1 (https=) |
| JP (3) | JP6718813B2 (https=) |
| KR (2) | KR102481330B1 (https=) |
| CN (2) | CN110819658B (https=) |
| AU (4) | AU2014287397B2 (https=) |
| BR (1) | BR112016000571B1 (https=) |
| CA (2) | CA3218040A1 (https=) |
| IL (3) | IL282489B (https=) |
| RU (3) | RU2748433C2 (https=) |
| SG (3) | SG10201913015XA (https=) |
| WO (1) | WO2015006294A2 (https=) |
Families Citing this family (136)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2013010911A (es) | 2011-03-23 | 2015-03-03 | Pioneer Hi Bred Int | Metodos para producir un locus de rasgo transgenico complejo. |
| JP6261500B2 (ja) | 2011-07-22 | 2018-01-17 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ヌクレアーゼ切断特異性の評価および改善 |
| EP3241902B1 (en) | 2012-05-25 | 2018-02-28 | The Regents of The University of California | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| EP3363902B1 (en) | 2012-12-06 | 2019-11-27 | Sigma Aldrich Co. LLC | Crispr-based genome modification and regulation |
| CA2895155C (en) * | 2012-12-17 | 2021-07-06 | President And Fellows Of Harvard College | Rna-guided human genome engineering |
| SI2986729T1 (sl) | 2013-04-16 | 2019-02-28 | Regeneron Pharmaceuticals, Inc. | Ciljana sprememba genoma podgane |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| CA3109801C (en) | 2013-08-22 | 2024-01-09 | Andrew Cigan | Plant genome modification using guide rna/cas endonuclease systems and methods of use |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| EP3418379B1 (en) | 2013-09-18 | 2020-12-09 | Kymab Limited | Methods, cells & organisms |
| US11326209B2 (en) * | 2013-11-07 | 2022-05-10 | Massachusetts Institute Of Technology | Cell-based genomic recorded accumulative memory |
| AU2014346559B2 (en) | 2013-11-07 | 2020-07-09 | Editas Medicine,Inc. | CRISPR-related methods and compositions with governing gRNAs |
| US9546384B2 (en) | 2013-12-11 | 2017-01-17 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for the targeted modification of a mouse genome |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| WO2015134812A1 (en) | 2014-03-05 | 2015-09-11 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for treating usher syndrome and retinitis pigmentosa |
| WO2015138510A1 (en) | 2014-03-10 | 2015-09-17 | Editas Medicine., Inc. | Crispr/cas-related methods and compositions for treating leber's congenital amaurosis 10 (lca10) |
| US11339437B2 (en) | 2014-03-10 | 2022-05-24 | Editas Medicine, Inc. | Compositions and methods for treating CEP290-associated disease |
| US11141493B2 (en) | 2014-03-10 | 2021-10-12 | Editas Medicine, Inc. | Compositions and methods for treating CEP290-associated disease |
| US11242525B2 (en) | 2014-03-26 | 2022-02-08 | Editas Medicine, Inc. | CRISPR/CAS-related methods and compositions for treating sickle cell disease |
| US12460231B2 (en) | 2014-04-02 | 2025-11-04 | Editas Medicine, Inc. | Crispr/CAS-related methods and compositions for treating primary open angle glaucoma |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| CN108064129A (zh) | 2014-09-12 | 2018-05-22 | 纳幕尔杜邦公司 | 玉米和大豆中复合性状基因座的位点特异性整合位点的产生和使用方法 |
| EP3204496A1 (en) | 2014-10-10 | 2017-08-16 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| GB201418965D0 (https=) | 2014-10-24 | 2014-12-10 | Ospedale San Raffaele And Fond Telethon | |
| CA2963820A1 (en) | 2014-11-07 | 2016-05-12 | Editas Medicine, Inc. | Methods for improving crispr/cas-mediated genome-editing |
| US10900034B2 (en) | 2014-12-03 | 2021-01-26 | Agilent Technologies, Inc. | Guide RNA with chemical modifications |
| AU2015360502A1 (en) | 2014-12-10 | 2017-06-29 | Regents Of The University Of Minnesota | Genetically modified cells, tissues, and organs for treating disease |
| BR112017017260A2 (pt) | 2015-03-27 | 2018-04-17 | Du Pont | construções de dna, vetor, célula, plantas, semente, método de expressão de rna e método de modificação de local alvo |
| EP3280803B1 (en) | 2015-04-06 | 2021-05-26 | The Board of Trustees of the Leland Stanford Junior University | Chemically modified guide rnas for crispr/cas-mediated gene regulation |
| CA2982966C (en) * | 2015-04-24 | 2024-02-20 | Editas Medicine, Inc. | Evaluation of cas9 molecule/guide rna molecule complexes |
| AU2016261358B2 (en) | 2015-05-11 | 2021-09-16 | Editas Medicine, Inc. | Optimized CRISPR/Cas9 systems and methods for gene editing in stem cells |
| CN107849546A (zh) * | 2015-05-15 | 2018-03-27 | 先锋国际良种公司 | 对cas内切核酸酶系统、pam序列和指导rna元件的快速表征 |
| EA037359B1 (ru) | 2015-06-01 | 2021-03-17 | Тэмпл Юниверсити - Оф Зе Коммонвэлс Систем Оф Хайе Эдьюкейшн | Способы и композиции для рнк-направляемого лечения инфекции, вызываемой вич |
| EP3307887A1 (en) | 2015-06-09 | 2018-04-18 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for improving transplantation |
| WO2016205745A2 (en) * | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Cell sorting |
| GB2592821B (en) | 2015-07-31 | 2022-01-12 | Univ Minnesota | Modified cells and methods of therapy |
| AU2016326711B2 (en) | 2015-09-24 | 2022-11-03 | Editas Medicine, Inc. | Use of exonucleases to improve CRISPR/Cas-mediated genome editing |
| JP7109784B2 (ja) | 2015-10-23 | 2022-08-01 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 遺伝子編集のための進化したＣａｓ９蛋白質 |
| WO2017074943A1 (en) * | 2015-10-27 | 2017-05-04 | The Board Of Trustees Of The Leland Stanford Junior University | Methods of inducibly targeting chromatin effectors and compositions for use in the same |
| JP2019500899A (ja) * | 2015-11-23 | 2019-01-17 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | ＣＲＩＳＰＲ／Ｃａｓ９の核送達を通じた細胞ＲＮＡの追跡と操作 |
| WO2017123559A2 (en) | 2016-01-11 | 2017-07-20 | The Board Of Trustees Of The Leland Stanford Junior University | Chimeric proteins and methods of regulating gene expression |
| ES2875747T3 (es) | 2016-01-11 | 2021-11-11 | Univ Leland Stanford Junior | Proteínas quiméricas y métodos de inmunoterapia |
| US11254928B2 (en) | 2016-01-15 | 2022-02-22 | Astrazeneca Ab | Gene modification assays |
| EP3433363A1 (en) | 2016-03-25 | 2019-01-30 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| WO2017165862A1 (en) | 2016-03-25 | 2017-09-28 | Editas Medicine, Inc. | Systems and methods for treating alpha 1-antitrypsin (a1at) deficiency |
| US12559567B2 (en) | 2016-04-12 | 2026-02-24 | Carrygenes Bioengineering, Llc | Methods for creating synthetic chromosomes having gene regulatory systems and uses thereof |
| CN109328232A (zh) | 2016-04-12 | 2019-02-12 | 辛普洛德生物技术有限责任公司 | 用于产生表达生物合成通路的合成染色体的方法及其用途 |
| WO2017180694A1 (en) | 2016-04-13 | 2017-10-19 | Editas Medicine, Inc. | Cas9 fusion molecules gene editing systems, and methods of use thereof |
| US10767175B2 (en) | 2016-06-08 | 2020-09-08 | Agilent Technologies, Inc. | High specificity genome editing using chemically modified guide RNAs |
| WO2017222834A1 (en) * | 2016-06-10 | 2017-12-28 | City Of Hope | Compositions and methods for mitochondrial genome editing |
| WO2018026976A1 (en) | 2016-08-02 | 2018-02-08 | Editas Medicine, Inc. | Compositions and methods for treating cep290 associated disease |
| KR102547316B1 (ko) | 2016-08-03 | 2023-06-23 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| CN106282228A (zh) * | 2016-08-19 | 2017-01-04 | 苏州兰希亚生物科技有限公司 | 一种基因点突变修复的方法 |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| US20190225974A1 (en) | 2016-09-23 | 2019-07-25 | BASF Agricultural Solutions Seed US LLC | Targeted genome optimization in plants |
| EP3526320A1 (en) | 2016-10-14 | 2019-08-21 | President and Fellows of Harvard College | Aav delivery of nucleobase editors |
| JP7181862B2 (ja) * | 2016-10-18 | 2022-12-01 | リージェンツ オブ ザ ユニバーシティ オブ ミネソタ | 腫瘍浸潤リンパ球および治療の方法 |
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