JP2016513478A5 - - Google Patents

Info

Publication number

JP2016513478A5

JP2016513478A5 JP2016503216A JP2016503216A JP2016513478A5 JP 2016513478 A5 JP2016513478 A5 JP 2016513478A5 JP 2016503216 A JP2016503216 A JP 2016503216A JP 2016503216 A JP2016503216 A JP 2016503216A JP 2016513478 A5 JP2016513478 A5 JP 2016513478A5

Authority

JP

Japan

Prior art keywords

cell culture

culture medium

insulin

cystine

copper

Prior art date

2014-03-14

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Pending

Links

• Espacenet
• Global Dossier
• Discuss

• NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 64
• 238000000034 method Methods 0.000 claims 57
• 239000006143 cell culture medium Substances 0.000 claims 49
• 102000004877 Insulin Human genes 0.000 claims 32
• 108090001061 Insulin Proteins 0.000 claims 32
• 229940125396 insulin Drugs 0.000 claims 32
• LEVWYRKDKASIDU-QWWZWVQMSA-N D-cystine Chemical compound OC(=O)[C@H](N)CSSC[C@@H](N)C(O)=O LEVWYRKDKASIDU-QWWZWVQMSA-N 0.000 claims 31
• 229960003067 cystine Drugs 0.000 claims 31
• 229960000397 bevacizumab Drugs 0.000 claims 21
• 239000012634 fragment Substances 0.000 claims 21
• RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims 20
• 229940108928 copper Drugs 0.000 claims 20
• 229910052802 copper Inorganic materials 0.000 claims 20
• 239000010949 copper Substances 0.000 claims 20
• 210000004962 mammalian cell Anatomy 0.000 claims 16
• 238000004113 cell culture Methods 0.000 claims 14
• 210000004027 cell Anatomy 0.000 claims 10
• 238000012258 culturing Methods 0.000 claims 9
• 150000007523 nucleic acids Chemical class 0.000 claims 8
• 102000039446 nucleic acids Human genes 0.000 claims 8
• 108020004707 nucleic acids Proteins 0.000 claims 8
• 239000002609 medium Substances 0.000 claims 4
• XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 3
• 235000018417 cysteine Nutrition 0.000 claims 3
• 241001465754 Metazoa Species 0.000 claims 2
• 238000004519 manufacturing process Methods 0.000 claims 2
• 230000010261 cell growth Effects 0.000 claims 1
• 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 1
• 230000001186 cumulative effect Effects 0.000 claims 1
• 230000002708 enhancing effect Effects 0.000 claims 1
• 239000001963 growth medium Substances 0.000 claims 1
• 230000000977 initiatory effect Effects 0.000 claims 1