BR112022012109A2 - Célula de levedura capaz de produzir e8,e10-dodecadienil coenzima a e opcionalmente e8,e10-dodecadien-1-ol, e, método para produzir e8,e10-dodecadienil coenzima a e opcionalmente e8,e10-dodecadien-1-ol em uma célula de levedura - Google Patents

Célula de levedura capaz de produzir e8,e10-dodecadienil coenzima a e opcionalmente e8,e10-dodecadien-1-ol, e, método para produzir e8,e10-dodecadienil coenzima a e opcionalmente e8,e10-dodecadien-1-ol em uma célula de levedura

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Publication number
BR112022012109A2
BR112022012109A2 BR112022012109A BR112022012109A BR112022012109A2 BR 112022012109 A2 BR112022012109 A2 BR 112022012109A2 BR 112022012109 A BR112022012109 A BR 112022012109A BR 112022012109 A BR112022012109 A BR 112022012109A BR 112022012109 A2 BR112022012109 A2 BR 112022012109A2
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BR
Brazil
Prior art keywords
dodecadienyl
dodecadien
coenzyme
producing
yeast
Prior art date
Application number
BR112022012109A
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English (en)
Inventor
Borodina Irina
Holkenbrink Carina
Löfstedt Christer
Ding Baojian
Wenning Leonie
Original Assignee
Biophero Aps
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Publication date
Application filed by Biophero Aps filed Critical Biophero Aps
Publication of BR112022012109A2 publication Critical patent/BR112022012109A2/pt

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    • C—CHEMISTRY; METALLURGY
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/32—Yeast
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/004—Cytochrome-b5 reductase (1.6.2.2)
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    • C12N9/0004—Oxidoreductases (1.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01084—Alcohol-forming fatty acyl-CoA reductase (1.2.1.84)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12Y106/02002—Cytochrome-b5 reductase (1.6.2.2)
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    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01086—Fatty-acyl-CoA synthase (2.3.1.86)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

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  • Life Sciences & Earth Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Zoology (AREA)
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  • Wood Science & Technology (AREA)
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  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

CÉLULA DE LEVEDURA CAPAZ DE PRODUZIR E8,E10- DODECADIENIL COENZIMA A E OPCIONALMENTE E8,E10- DODECADIEN-1-OL, E, MÉTODO PARA PRODUZIR E8,E10- DODECADIENIL COENZIMA A E OPCIONALMENTE E8,E10- DODECADIEN-1-OL EM UMA CÉLULA DE LEVEDURA. A presente invenção refere-se a células de levedura modificadas para a produção de E8,E10-dodecadienil coenzima A, codlemone (E8,E10-dodecadien-1-ol) e, opcionalmente, seus derivados E8,E10-dodecadienil acetato e/ou E8,E10-dodecadienal. Métodos para produção de E8,E10-dodecadienil coenzima A, codlemone (E8,E10-dodecadien-1-ol) e, opcionalmente, seus derivados E8,E10-dodecadienil acetato e/ou E8,E10-dodecadienal também são providos. Construções de ácidos nucleicos úteis para obter tais células de levedura também são providas.
BR112022012109A 2019-12-20 2020-12-18 Célula de levedura capaz de produzir e8,e10-dodecadienil coenzima a e opcionalmente e8,e10-dodecadien-1-ol, e, método para produzir e8,e10-dodecadienil coenzima a e opcionalmente e8,e10-dodecadien-1-ol em uma célula de levedura BR112022012109A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19218703 2019-12-20
PCT/EP2020/086975 WO2021123128A1 (en) 2019-12-20 2020-12-18 Yeast cells and methods for production of e8,e10-dodecadienyl coenzyme a, codlemone and derivatives thereof

Publications (1)

Publication Number Publication Date
BR112022012109A2 true BR112022012109A2 (pt) 2022-12-13

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BR112022012109A BR112022012109A2 (pt) 2019-12-20 2020-12-18 Célula de levedura capaz de produzir e8,e10-dodecadienil coenzima a e opcionalmente e8,e10-dodecadien-1-ol, e, método para produzir e8,e10-dodecadienil coenzima a e opcionalmente e8,e10-dodecadien-1-ol em uma célula de levedura

Country Status (12)

Country Link
US (1) US20240327874A1 (pt)
EP (1) EP4077636A1 (pt)
JP (1) JP2023507647A (pt)
KR (1) KR20220118442A (pt)
CN (1) CN115103900A (pt)
AU (1) AU2020407273A1 (pt)
BR (1) BR112022012109A2 (pt)
CA (1) CA3161539A1 (pt)
CL (1) CL2022001679A1 (pt)
IL (1) IL293960A (pt)
MX (1) MX2022007696A (pt)
WO (1) WO2021123128A1 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111690587B (zh) * 2019-03-13 2022-10-25 上海凯赛生物技术股份有限公司 一种离心筛选具有高含油率油脂酵母菌株的方法及其应用
CN112410355B (zh) * 2020-11-23 2022-03-25 昆明理工大学 一种酰基辅酶a氧化酶2基因rkacox2及其应用
CA3225388A1 (en) 2021-08-06 2023-02-09 Anders Gabrielsson Method for producing fatty aldehydes and derivatives thereof
AR128802A1 (es) 2022-03-16 2024-06-12 Biophero Aps Estabilización de aldehídos y/o alcoholes
TW202409274A (zh) 2022-07-04 2024-03-01 丹麥商百歐飛羅公司 生物農藥組成物

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108138202B (zh) * 2015-06-26 2023-05-16 丹麦科技大学 用于在酵母中产生蛾信息素的方法
US11434506B2 (en) * 2016-12-16 2022-09-06 Danmarks Tekniske Universitet Production of desaturated fatty alcohols and desaturated fatty alcohol acetates in yeast
DE17825464T1 (de) 2016-12-16 2019-12-19 Danmarks Tekniske Universitet Verfahren zur herstellung von fettalkoholen und derivaten davon in hefe
AR112543A1 (es) * 2017-05-17 2019-11-13 Provivi Inc Microorganismos para la producción de feromonas de insectos y compuestos relacionados
US20230332096A1 (en) 2019-02-19 2023-10-19 Biophero Aps Methods and cell factories for producing insect pheromones

Also Published As

Publication number Publication date
US20240327874A1 (en) 2024-10-03
WO2021123128A1 (en) 2021-06-24
JP2023507647A (ja) 2023-02-24
AU2020407273A1 (en) 2022-07-14
KR20220118442A (ko) 2022-08-25
MX2022007696A (es) 2022-09-23
CL2022001679A1 (es) 2023-02-24
IL293960A (en) 2022-08-01
EP4077636A1 (en) 2022-10-26
CN115103900A (zh) 2022-09-23
CA3161539A1 (en) 2021-06-24

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