JP2015529451A5 - - Google Patents

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JP2015529451A5
JP2015529451A5 JP2015522172A JP2015522172A JP2015529451A5 JP 2015529451 A5 JP2015529451 A5 JP 2015529451A5 JP 2015522172 A JP2015522172 A JP 2015522172A JP 2015522172 A JP2015522172 A JP 2015522172A JP 2015529451 A5 JP2015529451 A5 JP 2015529451A5
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Japan
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amino acid
helicase
construct
seq
polynucleotide
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JP2015522172A
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Japanese (ja)
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JP2015529451A (ja
JP6429773B2 (ja
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Priority claimed from PCT/GB2013/051928 external-priority patent/WO2014013262A1/en
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Publication of JP2015529451A5 publication Critical patent/JP2015529451A5/ja
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JP2015522172A 2012-07-19 2013-07-18 酵素構築物 Active JP6429773B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261673446P 2012-07-19 2012-07-19
US61/673,446 2012-07-19
US201361774694P 2013-03-08 2013-03-08
US61/774,694 2013-03-08
PCT/GB2013/051928 WO2014013262A1 (en) 2012-07-19 2013-07-18 Enzyme construct

Related Child Applications (1)

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JP2018146743A Division JP2018186830A (ja) 2012-07-19 2018-08-03 酵素構築物

Publications (3)

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JP2015529451A JP2015529451A (ja) 2015-10-08
JP2015529451A5 true JP2015529451A5 (enExample) 2016-09-01
JP6429773B2 JP6429773B2 (ja) 2018-11-28

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JP2015522172A Active JP6429773B2 (ja) 2012-07-19 2013-07-18 酵素構築物
JP2018146743A Pending JP2018186830A (ja) 2012-07-19 2018-08-03 酵素構築物

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JP2018146743A Pending JP2018186830A (ja) 2012-07-19 2018-08-03 酵素構築物

Country Status (7)

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US (2) US9797009B2 (enExample)
EP (1) EP2875152B1 (enExample)
JP (2) JP6429773B2 (enExample)
AU (1) AU2013291765C1 (enExample)
BR (1) BR112015001052B8 (enExample)
CA (1) CA2879355C (enExample)
WO (1) WO2014013262A1 (enExample)

Families Citing this family (93)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ579083A (en) 2007-02-20 2012-07-27 Oxford Nanopore Tech Ltd Lipid bilayer sensor system
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
JP5843614B2 (ja) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
AU2012264497B2 (en) 2011-05-27 2017-06-15 Oxford Nanopore Technologies Limited Coupling method
JP6298404B2 (ja) 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
WO2013041878A1 (en) 2011-09-23 2013-03-28 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
KR20130049671A (ko) 2011-11-04 2013-05-14 한미사이언스 주식회사 생리활성 폴리펩타이드 결합체 제조 방법
KR102086182B1 (ko) 2011-12-29 2020-03-06 옥스포드 나노포어 테크놀로지즈 리미티드 효소 방법
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
AU2013220156B2 (en) 2012-02-15 2018-08-09 Oxford Nanopore Technologies Limited Aptamer method
EP3736339B1 (en) 2012-02-16 2022-07-27 Oxford Nanopore Technologies plc Analysis of measurements of a polymer
AR090281A1 (es) 2012-03-08 2014-10-29 Hanmi Science Co Ltd Proceso mejorado para la preparacion de un complejo polipeptidico fisiologicamente activo
CN112646019B (zh) 2012-04-10 2022-08-16 牛津纳米孔科技公开有限公司 突变胞溶素孔
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
EP2895618B1 (en) 2012-09-14 2017-07-26 Oxford Nanopore Technologies Limited Sample preparation method
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
US9995728B2 (en) 2012-11-06 2018-06-12 Oxford Nanopore Technologies Ltd. Quadruplex method
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
AU2014224432B2 (en) 2013-03-08 2019-10-24 Oxford Nanopore Technologies Limited Enzyme stalling method
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CA2927726A1 (en) 2013-10-18 2015-04-23 Oxford Nanopore Technologies Limited Modified helicases for use in nucleic acid sequencing
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
EP3097210B2 (en) * 2014-01-22 2022-04-20 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
DK3127923T3 (da) * 2014-03-31 2021-11-22 Hanmi Pharm Ind Co Ltd Fremgangsmåde til forbedring af oppløselighed af protein og peptid ved hjælp af immunoglobulin-fc-fragment-binding
EP3126515B1 (en) 2014-04-04 2018-08-29 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
EP3137490B1 (en) 2014-05-02 2021-01-27 Oxford Nanopore Technologies Limited Mutant pores
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
CN115851894B (zh) 2014-10-16 2025-06-27 牛津楠路珀尔科技股份有限公司 聚合物的分析
GB201418512D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Electrical device with detachable components
CN107109489B (zh) 2014-10-17 2021-11-30 牛津纳米孔技术公司 纳米孔rna表征方法
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
EP3283887B1 (en) 2015-04-14 2021-07-21 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
AU2016369071B2 (en) 2015-12-08 2022-05-19 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
KR102222192B1 (ko) 2016-03-02 2021-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 기공
JP7364333B2 (ja) 2016-04-06 2023-10-18 オックスフォード ナノポール テクノロジーズ ピーエルシー 変異体ポア
WO2017203268A1 (en) 2016-05-25 2017-11-30 Oxford Nanopore Technologies Limited Method
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201611770D0 (en) 2016-07-06 2016-08-17 Oxford Nanopore Tech Microfluidic device
GB201616590D0 (en) 2016-09-29 2016-11-16 Oxford Nanopore Technologies Limited Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
KR102593484B1 (ko) 2017-06-29 2023-10-24 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 나노포어를 통한 폴리머의 결정적 스텝핑
CN117106037B (zh) 2017-06-30 2025-07-25 弗拉芒区生物技术研究所 新颖蛋白孔
GB2568895B (en) 2017-11-29 2021-10-27 Oxford Nanopore Tech Ltd Microfluidic device
GB2569977A (en) 2018-01-05 2019-07-10 Oxford Nanopore Tech Ltd Method
GB201801768D0 (en) 2018-02-02 2018-03-21 Oxford Nanopore Tech Ltd Synthesis method
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB201808554D0 (en) 2018-05-24 2018-07-11 Oxford Nanopore Tech Ltd Method
GB201808556D0 (en) 2018-05-24 2018-07-11 Oxford Nanopore Tech Ltd Method
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
US20220162692A1 (en) 2018-07-30 2022-05-26 Oxford University Innovation Limited Assemblies
EP3844497B1 (en) 2018-08-28 2025-11-12 F. Hoffmann-La Roche AG Nanopore sequencing device comprising ruthenium-containing electrodes
EP3898643B1 (en) 2018-12-19 2024-04-17 Roche Diagnostics GmbH 3' reversibly protected nucleotides for sequencing by synthesis using nanopores
GB201821155D0 (en) 2018-12-21 2019-02-06 Oxford Nanopore Tech Ltd Method
KR20210138594A (ko) 2019-03-12 2021-11-19 옥스포드 나노포어 테크놀로지즈 피엘씨 나노포어 감지 디바이스 및 이를 작동하는 방법 및 형성하는 방법
GB201907244D0 (en) * 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
WO2022013551A1 (en) 2020-07-17 2022-01-20 Oxford Nanopore Technologies Limited Nanopore sensing device
CN117337333A (zh) 2021-05-19 2024-01-02 牛津纳米孔科技公开有限公司 用于补体链测序的方法
GB202112235D0 (en) 2021-08-26 2021-10-13 Oxford Nanopore Tech Ltd Nanopore
GB202205617D0 (en) 2022-04-14 2022-06-01 Oxford Nanopore Tech Plc Novel modified protein pores and enzymes
EP4519453A1 (en) 2022-05-06 2025-03-12 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
KR20250044691A (ko) 2022-08-09 2025-04-01 옥스포드 나노포어 테크놀로지즈 피엘씨 신규 포어 단량체 및 포어
GB202211607D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202211602D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
EP4608850A2 (en) 2022-10-28 2025-09-03 Oxford Nanopore Technologies PLC Pore monomers and pores
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases
CN119060983A (zh) * 2023-06-02 2024-12-03 北京齐碳科技有限公司 解旋酶突变体及其应用
GB202315935D0 (en) 2023-10-18 2023-11-29 Oxford Nanopore Tech Ltd Novel enzymes
GB202405960D0 (en) 2024-04-29 2024-06-12 Oxford Nanopore Tech Plc Photo-active conjugates and their uses
GB202407100D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc A flow cell and a method
GB202407099D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc A flow cell and a method
GB202407044D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc Polymer analysis using transformer neural network
GB202407055D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc Enhanced polynucleotide sequencing
GB202407247D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6602979B1 (en) 1994-12-19 2003-08-05 The United States Of America As Represented By The Department Of Health And Human Services Screening assays for compounds that cause apoptosis
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
EP1384783A4 (en) * 2001-05-01 2005-04-20 Nat Inst Of Advanced Ind Scien NEW MAXIZYM
AU2003272438B2 (en) * 2002-09-20 2009-04-02 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
JP4757804B2 (ja) 2004-10-27 2011-08-24 株式会社カネカ 新規カルボニル還元酵素、その遺伝子、およびその利用法
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
NZ579083A (en) 2007-02-20 2012-07-27 Oxford Nanopore Tech Ltd Lipid bilayer sensor system
WO2009035647A1 (en) 2007-09-12 2009-03-19 President And Fellows Of Harvard College High-resolution molecular graphene sensor comprising an aperture in the graphene layer
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US8231969B2 (en) 2008-03-26 2012-07-31 University Of Utah Research Foundation Asymmetrically functionalized nanoparticles
US9447152B2 (en) 2008-07-07 2016-09-20 Oxford Nanopore Technologies Limited Base-detecting pore
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
EP3029467B1 (en) 2008-09-22 2020-01-08 University of Washington Msp nanopores and related methods
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
CA2750874A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Hybridization linkers
JP5843614B2 (ja) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
AU2010215761B2 (en) 2009-02-23 2017-04-06 Cytomx Therapeutics, Inc Proproteins and methods of use thereof
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
JP5781502B2 (ja) 2009-04-20 2015-09-24 オックスフォード ナノポール テクノロジーズ リミテッド 脂質二重層センサアレイ
BR112012013074B1 (pt) 2009-12-01 2018-09-18 Oxford Nanopore Technologies Limited instrumento de análise e módulo para realizar análise bioquímica, e, método para operar um instrumento de análise para realizar análise bioquímica
WO2012033524A2 (en) 2010-09-07 2012-03-15 The Regents Of The University Of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
US20130291392A1 (en) 2011-01-18 2013-11-07 R.K. Swamy Multipurpose instrument for triangle solutions, measurements and geometrical applications called triometer
US9402808B2 (en) 2011-01-19 2016-08-02 Panacea Biotec Limited Liquid oral composition of lanthanum salts
KR101939420B1 (ko) 2011-02-11 2019-01-16 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 세공
AU2012264497B2 (en) 2011-05-27 2017-06-15 Oxford Nanopore Technologies Limited Coupling method
JP6298404B2 (ja) 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
WO2013041878A1 (en) 2011-09-23 2013-03-28 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
KR102086182B1 (ko) 2011-12-29 2020-03-06 옥스포드 나노포어 테크놀로지즈 리미티드 효소 방법
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
CN112646019B (zh) 2012-04-10 2022-08-16 牛津纳米孔科技公开有限公司 突变胞溶素孔
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
EP4039733A1 (en) 2013-03-14 2022-08-10 Arkema, Inc. Methods for crosslinking polymer compositions in the presence of atmospheric oxygen
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CA2927726A1 (en) 2013-10-18 2015-04-23 Oxford Nanopore Technologies Limited Modified helicases for use in nucleic acid sequencing
US9925679B2 (en) 2014-05-19 2018-03-27 I+D+M Creative, Llc Devices and methods for assisting with slicing items

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