JP6429773B2 - 酵素構築物 - Google Patents

酵素構築物 Download PDF

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Publication number
JP6429773B2
JP6429773B2 JP2015522172A JP2015522172A JP6429773B2 JP 6429773 B2 JP6429773 B2 JP 6429773B2 JP 2015522172 A JP2015522172 A JP 2015522172A JP 2015522172 A JP2015522172 A JP 2015522172A JP 6429773 B2 JP6429773 B2 JP 6429773B2
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seq
helicase
amino acid
polynucleotide
sequence
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JP2015529451A5 (enExample
JP2015529451A (ja
Inventor
ヘロン，アンドリュー
クラーク，ジェームス
モイセイ，ルース
ジェーン ウォレス，エリザベス
ジェーン ウォレス，エリザベス
ブルース，マーク
ジャヤシンゲ，ラクマル
カプロッティ，ドメニコ
ソエロエス，サボルチ
マクニール，ルーク
ミッシャ，ミハエラ
Original Assignee
オックスフォード ナノポール テクノロジーズ リミテッド
オックスフォード ナノポール テクノロジーズ リミテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/04—Hydrolases acting on acid anhydrides (3.6) acting on acid anhydrides; involved in cellular and subcellular movement (3.6.4)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
JP2015522172A 2012-07-19 2013-07-18 酵素構築物 Active JP6429773B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261673446P 2012-07-19 2012-07-19
US61/673,446 2012-07-19
US201361774694P 2013-03-08 2013-03-08
US61/774,694 2013-03-08
PCT/GB2013/051928 WO2014013262A1 (en) 2012-07-19 2013-07-18 Enzyme construct

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2018146743A Division JP2018186830A (ja) 2012-07-19 2018-08-03 酵素構築物

Publications (3)

Publication Number Publication Date
JP2015529451A JP2015529451A (ja) 2015-10-08
JP2015529451A5 JP2015529451A5 (enExample) 2016-09-01
JP6429773B2 true JP6429773B2 (ja) 2018-11-28

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JP2015522172A Active JP6429773B2 (ja) 2012-07-19 2013-07-18 酵素構築物
JP2018146743A Pending JP2018186830A (ja) 2012-07-19 2018-08-03 酵素構築物

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JP2018146743A Pending JP2018186830A (ja) 2012-07-19 2018-08-03 酵素構築物

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US (2) US9797009B2 (enExample)
EP (1) EP2875152B1 (enExample)
JP (2) JP6429773B2 (enExample)
AU (1) AU2013291765C1 (enExample)
BR (1) BR112015001052B8 (enExample)
CA (1) CA2879355C (enExample)
WO (1) WO2014013262A1 (enExample)

Families Citing this family (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100196203A1 (en) 2007-02-20 2010-08-05 Gurdial Singh Sanghera Formation of Lipid Bilayers
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
WO2010086622A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
EP3633370B1 (en) 2011-05-27 2024-05-01 Oxford Nanopore Technologies plc Method and apparatus for determining the presence, absence or characteristics of an analyte
IN2014DN00221A (enExample) 2011-07-25 2015-06-05 Oxford Nanopore Tech Ltd
CA3113287C (en) 2011-09-23 2022-12-20 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
EP2987870B1 (en) 2011-10-21 2020-02-19 Oxford Nanopore Technologies Limited Method of characterizing a target polynucleotide using a transmembrane pore and molecular motor
KR20130049671A (ko) 2011-11-04 2013-05-14 한미사이언스 주식회사 생리활성 폴리펩타이드 결합체 제조 방법
WO2013098562A2 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Enzyme method
WO2013098561A1 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
EP2815235B1 (en) 2012-02-15 2020-04-15 Oxford Nanopore Technologies Limited Aptamer method
CA2866587C (en) 2012-02-16 2022-07-26 Oxford Nanopore Technologies Limited Analysis of measurements of a polymer
AR090281A1 (es) 2012-03-08 2014-10-29 Hanmi Science Co Ltd Proceso mejorado para la preparacion de un complejo polipeptidico fisiologicamente activo
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
JP6429773B2 (ja) 2012-07-19 2018-11-28 オックスフォード ナノポール テクノロジーズ リミテッド 酵素構築物
US10808231B2 (en) 2012-07-19 2020-10-20 Oxford Nanopore Technologies Limited Modified helicases
WO2014013259A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
WO2014041337A1 (en) 2012-09-14 2014-03-20 Oxford Nanopore Technologies Limited Sample preparation method
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
WO2014072703A1 (en) 2012-11-06 2014-05-15 Oxford Nanopore Technologies Limited Quadruplex method
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
BR112015021788B1 (pt) 2013-03-08 2023-02-28 Oxford Nanopore Technologies Plc Métodos para mover uma ou mais helicases imobilizadas, para controlar o movimento de um polinucleotídeo alvo, para caracterizar um polinucleotídeo alvo e para controlar o carregamento de uma ou mais helicases em um polinucleotídeo alvo, uso de um poro de transmembrana e de um potencial aplicado e de um ou mais espaçadores, complexo, e, kit
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
GB201406151D0 (en) * 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CN117947149A (zh) 2013-10-18 2024-04-30 牛津纳米孔科技公开有限公司 经修饰的酶
CA2937411C (en) 2014-01-22 2023-09-26 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
UA130573C2 (uk) * 2014-03-31 2026-03-25 Ханмі Фарм. Ко., Лтд. Спосіб покращення розчинності білка та пептиду із застосуванням зв'язування з fc-фрагментом імуноглобуліну
EP3126515B1 (en) 2014-04-04 2018-08-29 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
EP3137627B1 (en) 2014-05-02 2025-07-30 Oxford Nanopore Technologies plc Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
KR20170042794A (ko) 2014-09-01 2017-04-19 브이아이비 브이지더블유 돌연변이체 csgg 포어
WO2016055778A1 (en) 2014-10-07 2016-04-14 Oxford Nanopore Technologies Limited Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
EP3207154B1 (en) 2014-10-16 2022-03-16 Oxford Nanopore Technologies PLC Analysis of a polymer
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
CN107109489B (zh) 2014-10-17 2021-11-30 牛津纳米孔技术公司 纳米孔rna表征方法
GB201418512D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Electrical device with detachable components
GB201502810D0 (en) * 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
WO2016166232A1 (en) 2015-04-14 2016-10-20 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
AU2016369071B2 (en) 2015-12-08 2022-05-19 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
EP4122949B1 (en) 2016-04-06 2024-06-05 Oxford Nanopore Technologies plc Mutant pore
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN109196116B (zh) 2016-05-25 2022-09-27 牛津纳米孔科技公开有限公司 一种表征靶多核苷酸的方法
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201611770D0 (en) 2016-07-06 2016-08-17 Oxford Nanopore Tech Microfluidic device
GB201616590D0 (en) 2016-09-29 2016-11-16 Oxford Nanopore Technologies Limited Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
EP3646025B1 (en) 2017-06-29 2021-08-04 President and Fellows of Harvard College Deterministic stepping of polymers through a nanopore
CN117106037B (zh) 2017-06-30 2025-07-25 弗拉芒区生物技术研究所 新颖蛋白孔
GB2568895B (en) 2017-11-29 2021-10-27 Oxford Nanopore Tech Ltd Microfluidic device
GB2569977A (en) 2018-01-05 2019-07-10 Oxford Nanopore Tech Ltd Method
GB201801768D0 (en) 2018-02-02 2018-03-21 Oxford Nanopore Tech Ltd Synthesis method
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB201808556D0 (en) 2018-05-24 2018-07-11 Oxford Nanopore Tech Ltd Method
GB2574048B (en) 2018-05-24 2021-06-16 Oxford Nanopore Tech Ltd Nanopore sensor component with electrostatic discharge protection
GB201808554D0 (en) 2018-05-24 2018-07-11 Oxford Nanopore Tech Ltd Method
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
US20220162692A1 (en) 2018-07-30 2022-05-26 Oxford University Innovation Limited Assemblies
WO2020043653A2 (en) 2018-08-28 2020-03-05 F. Hoffmann-La Roche Ag Ruthenium-containing electrodes
ES2988083T3 (es) 2018-12-19 2024-11-19 Hoffmann La Roche Nucleótidos protegidos reversiblemente en 3' para secuenciación por síntesis usando nanoporos
GB201821155D0 (en) 2018-12-21 2019-02-06 Oxford Nanopore Tech Ltd Method
JP7492200B2 (ja) 2019-03-12 2024-05-29 オックスフォード ナノポール テクノロジーズ ピーエルシー ナノ細孔センシングデバイスと操作方法およびその成形方法
GB201907244D0 (en) * 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
EP4675256A3 (en) 2020-07-17 2026-01-28 Oxford Nanopore Technologies PLC Nanopore sensing device
EP4341433A1 (en) 2021-05-19 2024-03-27 Oxford Nanopore Technologies PLC Methods for complement strand sequencing
GB202112235D0 (en) 2021-08-26 2021-10-13 Oxford Nanopore Tech Ltd Nanopore
GB202205617D0 (en) 2022-04-14 2022-06-01 Oxford Nanopore Tech Plc Novel modified protein pores and enzymes
US12559791B2 (en) 2022-05-06 2026-02-24 10X Genomics, Inc. Methods and compositions for in situ analysis of V(D)J sequences
GB202211607D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
CA3262948A1 (en) 2022-08-09 2024-02-15 The Regents Of The University Of California NEW PORE-FORMING MONOMERS AND PORES
GB202211602D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
JP2026512988A (ja) 2022-10-28 2026-04-22 オックスフォード ナノポール テクノロジーズ ピーエルシー 細孔モノマーおよび細孔
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases
CN119060983A (zh) * 2023-06-02 2024-12-03 北京齐碳科技有限公司 解旋酶突变体及其应用
GB202315935D0 (en) 2023-10-18 2023-11-29 Oxford Nanopore Tech Ltd Novel enzymes
GB202405960D0 (en) 2024-04-29 2024-06-12 Oxford Nanopore Tech Plc Photo-active conjugates and their uses
GB202407099D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc A flow cell and a method
GB202407044D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc Polymer analysis using transformer neural network
GB202407100D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc A flow cell and a method
GB202407055D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc Enhanced polynucleotide sequencing
GB202407247D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method
GB202413506D0 (en) 2024-09-13 2024-10-30 Oxford Nanopore Tech Plc Pore monomers and their uses
GB2644084A (en) 2024-09-16 2026-03-18 Oxford Nanopore Tech Plc Augmented consensus and variant calling
GB202413618D0 (en) 2024-09-16 2024-10-30 Oxford Nanopore Tech Plc peptide characterisation
GB202413747D0 (en) 2024-09-18 2024-10-30 Oxford Nanopore Tech Plc Preprocessing nanopore signals

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6602979B1 (en) 1994-12-19 2003-08-05 The United States Of America As Represented By The Department Of Health And Human Services Screening assays for compounds that cause apoptosis
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US20040248114A1 (en) * 2001-05-01 2004-12-09 Kazunari Taira Novel maxizyme
DE60324810D1 (de) * 2002-09-20 2009-01-02 New England Biolabs Inc HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
WO2006046455A1 (ja) 2004-10-27 2006-05-04 Kaneka Corporation 新規カルボニル還元酵素、その遺伝子、およびその利用法
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
US20100196203A1 (en) 2007-02-20 2010-08-05 Gurdial Singh Sanghera Formation of Lipid Bilayers
US8698481B2 (en) 2007-09-12 2014-04-15 President And Fellows Of Harvard College High-resolution molecular sensor
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US8231969B2 (en) 2008-03-26 2012-07-31 University Of Utah Research Foundation Asymmetrically functionalized nanoparticles
WO2010004273A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
EP2307540B1 (en) 2008-07-07 2017-04-19 Oxford Nanopore Technologies Limited Enzyme-pore constructs
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
CN102216783B (zh) 2008-09-22 2015-04-01 华盛顿大学 Msp纳米微孔和相关方法
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
US20120100530A1 (en) 2009-01-30 2012-04-26 Oxford Nanopore Technologies Limited Enzyme mutant
WO2010086622A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
US8399219B2 (en) 2009-02-23 2013-03-19 Cytomx Therapeutics, Inc. Protease activatable interferon alpha proprotein
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
WO2010122293A1 (en) 2009-04-20 2010-10-28 Oxford Nanopore Technologies Limited Lipid bilayer sensor array
EP2507387B1 (en) 2009-12-01 2017-01-25 Oxford Nanopore Technologies Limited Biochemical analysis instrument and method
US20140051068A1 (en) 2010-09-07 2014-02-20 The Regents Of The University Of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
WO2012098561A2 (en) 2011-01-18 2012-07-26 Swamy R K A multipupurpose instrument for triangle solutions, measurements and geometrical applications called triometer
WO2012098562A2 (en) 2011-01-19 2012-07-26 Panacea Biotec Limited Liquid oral compositions of lanthanum salts
EP2673638B1 (en) 2011-02-11 2019-10-30 Oxford Nanopore Technologies Limited Mutant pores
EP3633370B1 (en) 2011-05-27 2024-05-01 Oxford Nanopore Technologies plc Method and apparatus for determining the presence, absence or characteristics of an analyte
IN2014DN00221A (enExample) 2011-07-25 2015-06-05 Oxford Nanopore Tech Ltd
CA3113287C (en) 2011-09-23 2022-12-20 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
EP2987870B1 (en) 2011-10-21 2020-02-19 Oxford Nanopore Technologies Limited Method of characterizing a target polynucleotide using a transmembrane pore and molecular motor
WO2013098561A1 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
WO2013098562A2 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Enzyme method
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
US10808231B2 (en) 2012-07-19 2020-10-20 Oxford Nanopore Technologies Limited Modified helicases
JP6429773B2 (ja) 2012-07-19 2018-11-28 オックスフォード ナノポール テクノロジーズ リミテッド 酵素構築物
WO2014013259A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
JP6484604B2 (ja) 2013-03-14 2019-03-13 アーケマ・インコーポレイテッド 大気酸素の存在下において重合体組成物を架橋させるための方法
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CN117947149A (zh) 2013-10-18 2024-04-30 牛津纳米孔科技公开有限公司 经修饰的酶
US9925679B2 (en) 2014-05-19 2018-03-27 I+D+M Creative, Llc Devices and methods for assisting with slicing items

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BR112015001052B8 (pt) 2022-12-27
AU2013291765B2 (en) 2019-05-16
US20180230526A1 (en) 2018-08-16
US9797009B2 (en) 2017-10-24
CA2879355C (en) 2021-09-21
EP2875152B1 (en) 2019-10-09
BR112015001052B1 (pt) 2022-04-05
WO2014013262A1 (en) 2014-01-23
US20150218629A1 (en) 2015-08-06
CA2879355A1 (en) 2014-01-23
AU2013291765C1 (en) 2019-08-08
JP2018186830A (ja) 2018-11-29
AU2013291765A1 (en) 2015-02-05
BR112015001052A2 (enExample) 2017-08-15
JP2015529451A (ja) 2015-10-08
EP2875152A1 (en) 2015-05-27

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