JP2015528313A5 - - Google Patents

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Publication number
JP2015528313A5
JP2015528313A5 JP2015532169A JP2015532169A JP2015528313A5 JP 2015528313 A5 JP2015528313 A5 JP 2015528313A5 JP 2015532169 A JP2015532169 A JP 2015532169A JP 2015532169 A JP2015532169 A JP 2015532169A JP 2015528313 A5 JP2015528313 A5 JP 2015528313A5
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JP
Japan
Prior art keywords
nucleic acid
metal salt
lysate
biological sample
salt
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2015532169A
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English (en)
Japanese (ja)
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JP2015528313A (ja
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Publication date
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Priority claimed from PCT/US2013/060349 external-priority patent/WO2014047141A1/en
Publication of JP2015528313A publication Critical patent/JP2015528313A/ja
Publication of JP2015528313A5 publication Critical patent/JP2015528313A5/ja
Pending legal-status Critical Current

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JP2015532169A 2012-09-19 2013-09-18 核酸の抽出における二価イオン、プロテアーゼ、洗剤、および低いｐｈの使用 Pending JP2015528313A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261703112P 2012-09-19 2012-09-19
US61/703,112 2012-09-19
PCT/US2013/060349 WO2014047141A1 (en) 2012-09-19 2013-09-18 USE OF DIVALENT IONS, PROTEASES, DETERGENTS, AND LOW pH IN THE EXTRACTION OF NUCLEIC ACIDS

Publications (2)

Publication Number Publication Date
JP2015528313A JP2015528313A (ja) 2015-09-28
JP2015528313A5 true JP2015528313A5 (https=) 2016-11-10

Family

ID=49304332

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015532169A Pending JP2015528313A (ja) 2012-09-19 2013-09-18 核酸の抽出における二価イオン、プロテアーゼ、洗剤、および低いｐｈの使用

Country Status (10)

Country Link
US (1) US20140087366A1 (https=)
EP (1) EP2898069B1 (https=)
JP (1) JP2015528313A (https=)
KR (1) KR20150054844A (https=)
CN (1) CN105008534A (https=)
AU (1) AU2013318156A1 (https=)
BR (1) BR112015005718A2 (https=)
IN (1) IN2015DN02479A (https=)
SG (1) SG11201502138WA (https=)
WO (1) WO2014047141A1 (https=)

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Publication number Priority date Publication date Assignee Title
US10233440B2 (en) 2012-09-28 2019-03-19 Cepheid Methods for DNA and RNA extraction from fixed paraffin-embedded tissue samples
CN104749009B (zh) * 2015-03-30 2018-05-04 上海云泽生物科技有限公司 用于免疫分析的免疫抑制剂药物提取试剂
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
KR101927720B1 (ko) * 2017-01-04 2018-12-11 국민대학교산학협력단 필터 추출용 조성물 및 이를 이용한 식품 내 미생물 분리 방법
CN108531472B (zh) * 2017-03-05 2021-07-16 北京新羿生物科技有限公司 一种用于核酸提取的裂解液
US11168323B2 (en) 2017-06-01 2021-11-09 Nantomics Llc DNA stabilization of RNA
CN107254463B (zh) * 2017-06-20 2018-11-20 迈克生物股份有限公司 用于提取丙型肝炎病毒rna的试剂盒
CN109306349A (zh) * 2017-07-26 2019-02-05 天津迦美惠众科技有限公司 一种基于纳米磁珠的生物样品核酸提取纯化方法
CN107794260A (zh) * 2017-11-21 2018-03-13 苏州吉玛基因股份有限公司 一种从大体积无细胞体液中提取游离核酸的方法
US12264311B2 (en) * 2019-01-25 2025-04-01 Sekisui Chemical Co., Ltd. Method for isolating nucleic acid, nucleic acid isolation kit, and inspection chip
CN111154753A (zh) * 2020-03-09 2020-05-15 重庆市大足区人民医院 一种磁珠法细菌核酸提取液
JP7723657B2 (ja) * 2020-05-25 2025-08-14 積水メディカル株式会社 核酸の精製方法
EP4215610A4 (en) * 2020-09-15 2024-10-16 Sekisui Medical Co., Ltd. NUCLEIC ACID PURIFICATION PROCESS, NUCLEIC ACID EXTRACTION FLUID AND NUCLEIC ACID PURIFICATION KIT
KR20220075978A (ko) * 2020-11-30 2022-06-08 (주)바이오니아 중저 비유전율을 가진 화합물을 포함한 핵산 결합 완충액을 이용한 핵산 분리방법
EP4267756A1 (en) * 2020-12-22 2023-11-01 Perkinelmer Health Sciences Inc. Detecting a target nucleic acid in a biological sample
WO2023150710A1 (en) * 2022-02-05 2023-08-10 Becton, Dickinson And Company Non-opaque lytic buffer composition formulations
CN114410747A (zh) * 2022-03-30 2022-04-29 北京中海生物科技有限公司 一种病毒免提试剂及其在病毒基因组扩增中的应用

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CA1301606C (en) * 1986-05-02 1992-05-26 David H. Gillespie Chaotropic method for evaluating nucleic acids in a biological sample
JP3856174B2 (ja) * 1997-12-11 2006-12-13 東洋紡績株式会社 植物ｄｎａの抽出精製方法
WO2001021830A1 (en) * 1999-09-24 2001-03-29 Ambion, Inc. Nuclease inhibitor cocktail
US20030203384A1 (en) * 2002-03-08 2003-10-30 Chafin David R. Multiplex detection of biological materials in a sample
US7998699B2 (en) * 2002-08-15 2011-08-16 University Of South Florida Early detection of pathogens in blood
EP1559783A1 (de) * 2004-01-29 2005-08-03 Qiagen GmbH Verfahren zur chromatographischen Trennung eines Nukleinsäuregemisches
JP5390772B2 (ja) * 2004-11-05 2014-01-15 キアゲン ノース アメリカン ホールディングス， インコーポレイテッド 核酸を安定化試薬から精製するための組成物および方法
CN101400689A (zh) * 2006-02-08 2009-04-01 鲁米金公司 提取rna的方法
US20070185322A1 (en) * 2006-02-08 2007-08-09 Nexgen Diagnostics Llc Methods of extracting RNA
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
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JP5641191B2 (ja) * 2010-02-26 2014-12-17 Jsr株式会社 ウイルスの検出方法
CA2780536C (en) * 2009-11-09 2018-01-02 Streck, Inc. Stabilization of rna in and extracting from intact cells within a blood sample
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US8420801B2 (en) * 2010-01-08 2013-04-16 Roche Molecular Systems, Inc. Recovery of nucleic acids from magnetic glass particles
CN103025891A (zh) * 2010-07-29 2013-04-03 霍夫曼-拉罗奇有限公司 通用样品制备

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