JP2015525567A5 - - Google Patents
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- Publication number
- JP2015525567A5 JP2015525567A5 JP2015524385A JP2015524385A JP2015525567A5 JP 2015525567 A5 JP2015525567 A5 JP 2015525567A5 JP 2015524385 A JP2015524385 A JP 2015524385A JP 2015524385 A JP2015524385 A JP 2015524385A JP 2015525567 A5 JP2015525567 A5 JP 2015525567A5
- Authority
- JP
- Japan
- Prior art keywords
- dna molecule
- dna
- sequence
- restriction enzyme
- type
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108020004414 DNA Proteins 0.000 claims description 57
- 102000053602 DNA Human genes 0.000 claims description 36
- 238000000034 method Methods 0.000 claims description 28
- 239000012634 fragment Substances 0.000 claims description 24
- 108091008146 restriction endonucleases Proteins 0.000 claims description 21
- 239000013598 vector Substances 0.000 claims description 16
- 108010091086 Recombinases Proteins 0.000 claims description 8
- 102000018120 Recombinases Human genes 0.000 claims description 8
- 239000003550 marker Substances 0.000 claims description 8
- 230000029087 digestion Effects 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 108700005090 Lethal Genes Proteins 0.000 claims description 4
- 108010010677 Phosphodiesterase I Proteins 0.000 claims description 4
- 229960000723 ampicillin Drugs 0.000 claims description 4
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 229930027917 kanamycin Natural products 0.000 claims description 4
- 229960000318 kanamycin Drugs 0.000 claims description 4
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 claims description 4
- 229930182823 kanamycin A Natural products 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 230000004544 DNA amplification Effects 0.000 claims description 2
- 230000006820 DNA synthesis Effects 0.000 claims description 2
- 238000012408 PCR amplification Methods 0.000 claims description 2
- 101150102092 ccdB gene Proteins 0.000 claims description 2
- 238000003752 polymerase chain reaction Methods 0.000 claims description 2
- 108020005345 3' Untranslated Regions Proteins 0.000 description 2
- 108010010574 Tn3 resolvase Proteins 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261675929P | 2012-07-26 | 2012-07-26 | |
| US61/675,929 | 2012-07-26 | ||
| PCT/US2013/051641 WO2014018512A1 (en) | 2012-07-26 | 2013-07-23 | High-throughput dna fragment assembly |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2015525567A JP2015525567A (ja) | 2015-09-07 |
| JP2015525567A5 true JP2015525567A5 (https=) | 2016-06-23 |
Family
ID=48914467
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015524385A Pending JP2015525567A (ja) | 2012-07-26 | 2013-07-23 | ハイスループットｄｎａフラグメントアセンブリ |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US9752154B2 (https=) |
| EP (1) | EP2877581B1 (https=) |
| JP (1) | JP2015525567A (https=) |
| KR (1) | KR20150032306A (https=) |
| CN (1) | CN104619847A (https=) |
| AU (1) | AU2013293123B2 (https=) |
| BR (1) | BR112015001316A2 (https=) |
| CA (1) | CA2882143A1 (https=) |
| IL (1) | IL236814A0 (https=) |
| IN (1) | IN2014DN11250A (https=) |
| RU (1) | RU2015106549A (https=) |
| WO (1) | WO2014018512A1 (https=) |
| ZA (1) | ZA201409509B (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016109981A1 (zh) * | 2015-01-09 | 2016-07-14 | 深圳华大基因研究院 | 一种dna合成产物的高通量检测方法 |
| CN106318961B (zh) * | 2015-06-30 | 2019-07-09 | 未名生物农业集团有限公司 | 一种多dna片段载体的构建方法及其应用 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4535060A (en) | 1983-01-05 | 1985-08-13 | Calgene, Inc. | Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use |
| US5380831A (en) | 1986-04-04 | 1995-01-10 | Mycogen Plant Science, Inc. | Synthetic insecticidal crystal protein gene |
| US4761373A (en) | 1984-03-06 | 1988-08-02 | Molecular Genetics, Inc. | Herbicide resistance in plants |
| US4940835A (en) | 1985-10-29 | 1990-07-10 | Monsanto Company | Glyphosate-resistant plants |
| KR950008571B1 (ko) | 1986-01-08 | 1995-08-03 | 롱쁠랑 아그로시미 | 할로아릴니트릴 분해 유전자, 그의 용도 및 이를 함유한 세포 |
| US4810648A (en) | 1986-01-08 | 1989-03-07 | Rhone Poulenc Agrochimie | Haloarylnitrile degrading gene, its use, and cells containing the gene |
| ATE57390T1 (de) | 1986-03-11 | 1990-10-15 | Plant Genetic Systems Nv | Durch gentechnologie erhaltene und gegen glutaminsynthetase-inhibitoren resistente pflanzenzellen. |
| US4975374A (en) | 1986-03-18 | 1990-12-04 | The General Hospital Corporation | Expression of wild type and mutant glutamine synthetase in foreign hosts |
| EP0333033A1 (en) | 1988-03-09 | 1989-09-20 | Meiji Seika Kaisha Ltd. | Glutamine synthesis gene and glutamine synthetase |
| CA2080584C (en) | 1990-04-18 | 2000-09-05 | Marc Cornelissen | Modified bacillus thuringiensis insecticidal-crystal protein genes and their expression in plant cells |
| US5633435A (en) | 1990-08-31 | 1997-05-27 | Monsanto Company | Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases |
| TW261517B (https=) | 1991-11-29 | 1995-11-01 | Mitsubishi Shozi Kk | |
| US7351578B2 (en) * | 1999-12-10 | 2008-04-01 | Invitrogen Corp. | Use of multiple recombination sites with unique specificity in recombinational cloning |
| US8877916B2 (en) * | 2000-04-26 | 2014-11-04 | Ceres, Inc. | Promoter, promoter control elements, and combinations, and uses thereof |
| WO2005012515A2 (en) | 2003-04-29 | 2005-02-10 | Pioneer Hi-Bred International, Inc. | Novel glyphosate-n-acetyltransferase (gat) genes |
| AU2715302A (en) * | 2000-12-08 | 2002-06-18 | Invitrogen Corp | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| BRPI0410342A (pt) * | 2003-05-05 | 2006-06-20 | Dow Agrosciences Llc | composições imunoprofiláticas e terapêuticas estáveis derivadas de células vegetais transgênicas e processos para produção |
| CN102094032B (zh) | 2004-04-30 | 2014-02-26 | 美国陶氏益农公司 | 新除草剂抗性基因 |
| ES2637948T3 (es) | 2005-10-28 | 2017-10-18 | Dow Agrosciences Llc | Nuevos genes de resistencia a herbicidas |
-
2013
- 2013-07-23 EP EP13744922.9A patent/EP2877581B1/en active Active
- 2013-07-23 US US14/416,799 patent/US9752154B2/en active Active
- 2013-07-23 AU AU2013293123A patent/AU2013293123B2/en not_active Ceased
- 2013-07-23 KR KR1020157001623A patent/KR20150032306A/ko not_active Withdrawn
- 2013-07-23 JP JP2015524385A patent/JP2015525567A/ja active Pending
- 2013-07-23 IN IN11250DEN2014 patent/IN2014DN11250A/en unknown
- 2013-07-23 CA CA2882143A patent/CA2882143A1/en not_active Abandoned
- 2013-07-23 CN CN201380039369.7A patent/CN104619847A/zh active Pending
- 2013-07-23 BR BR112015001316A patent/BR112015001316A2/pt not_active IP Right Cessation
- 2013-07-23 WO PCT/US2013/051641 patent/WO2014018512A1/en not_active Ceased
- 2013-07-23 RU RU2015106549A patent/RU2015106549A/ru not_active Application Discontinuation
-
2014
- 2014-12-23 ZA ZA2014/09509A patent/ZA201409509B/en unknown
-
2015
- 2015-01-20 IL IL236814A patent/IL236814A0/en unknown
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