JP2015523066A5 - - Google Patents
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- Publication number
- JP2015523066A5 JP2015523066A5 JP2015515191A JP2015515191A JP2015523066A5 JP 2015523066 A5 JP2015523066 A5 JP 2015523066A5 JP 2015515191 A JP2015515191 A JP 2015515191A JP 2015515191 A JP2015515191 A JP 2015515191A JP 2015523066 A5 JP2015523066 A5 JP 2015523066A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- acid sequence
- cleavage site
- flgm
- peptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 108090000765 processed proteins & peptides Proteins 0.000 claims 22
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 16
- 238000003776 cleavage reaction Methods 0.000 claims 16
- 150000007523 nucleic acids Chemical group 0.000 claims 16
- 230000007017 scission Effects 0.000 claims 16
- 210000004027 cell Anatomy 0.000 claims 12
- 229920001184 polypeptide Polymers 0.000 claims 11
- 102000004196 processed proteins & peptides Human genes 0.000 claims 11
- 238000000746 purification Methods 0.000 claims 11
- 108700029229 Transcriptional Regulatory Elements Proteins 0.000 claims 6
- 238000000034 method Methods 0.000 claims 5
- 101000844746 Drosophila melanogaster Drosomycin Proteins 0.000 claims 4
- 239000003053 toxin Substances 0.000 claims 4
- 231100000765 toxin Toxicity 0.000 claims 4
- 102100029727 Enteropeptidase Human genes 0.000 claims 3
- 108010013369 Enteropeptidase Proteins 0.000 claims 3
- 108010040721 Flagellin Proteins 0.000 claims 2
- 102000005720 Glutathione transferase Human genes 0.000 claims 2
- 108010070675 Glutathione transferase Proteins 0.000 claims 2
- 101710175625 Maltose/maltodextrin-binding periplasmic protein Proteins 0.000 claims 2
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 claims 2
- 241000723792 Tobacco etch virus Species 0.000 claims 2
- 239000003112 inhibitor Substances 0.000 claims 2
- 238000012986 modification Methods 0.000 claims 2
- 230000004048 modification Effects 0.000 claims 2
- 229920002704 polyhistidine Polymers 0.000 claims 2
- -1 srgD Proteins 0.000 claims 2
- SGKRLCUYIXIAHR-AKNGSSGZSA-N (4s,4ar,5s,5ar,6r,12ar)-4-(dimethylamino)-1,5,10,11,12a-pentahydroxy-6-methyl-3,12-dioxo-4a,5,5a,6-tetrahydro-4h-tetracene-2-carboxamide Chemical compound C1=CC=C2[C@H](C)[C@@H]([C@H](O)[C@@H]3[C@](C(O)=C(C(N)=O)C(=O)[C@H]3N(C)C)(O)C3=O)C3=C(O)C2=C1O SGKRLCUYIXIAHR-AKNGSSGZSA-N 0.000 claims 1
- 101100281124 Aquifex aeolicus (strain VF5) flaA gene Proteins 0.000 claims 1
- 101100173925 Caulobacter vibrioides (strain ATCC 19089 / CB15) fljL gene Proteins 0.000 claims 1
- 101100500579 Citrobacter freundii ecnR gene Proteins 0.000 claims 1
- 101100108073 Drosophila melanogaster Actn gene Proteins 0.000 claims 1
- 102000007474 Multiprotein Complexes Human genes 0.000 claims 1
- 108010085220 Multiprotein Complexes Proteins 0.000 claims 1
- 108091005804 Peptidases Proteins 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 108010076818 TEV protease Proteins 0.000 claims 1
- 239000004098 Tetracycline Substances 0.000 claims 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 claims 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims 1
- 210000004899 c-terminal region Anatomy 0.000 claims 1
- 101150074451 clpP gene Proteins 0.000 claims 1
- 101150043719 clpP1 gene Proteins 0.000 claims 1
- 101150102296 clpP2 gene Proteins 0.000 claims 1
- 101150096566 clpX gene Proteins 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 229960003722 doxycycline Drugs 0.000 claims 1
- 101150035430 fimZ gene Proteins 0.000 claims 1
- 210000003495 flagella Anatomy 0.000 claims 1
- 101150010879 flgK gene Proteins 0.000 claims 1
- 101150064216 flgL gene Proteins 0.000 claims 1
- 101150017109 fliA gene Proteins 0.000 claims 1
- 101150038062 fliC gene Proteins 0.000 claims 1
- 101150094936 fliD gene Proteins 0.000 claims 1
- 101150104647 fljB gene Proteins 0.000 claims 1
- 102000034356 gene-regulatory proteins Human genes 0.000 claims 1
- 108091006104 gene-regulatory proteins Proteins 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 101150061503 hdfR gene Proteins 0.000 claims 1
- 230000001939 inductive effect Effects 0.000 claims 1
- 101150016443 lrhA gene Proteins 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000002609 medium Substances 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 101150091444 ompR gene Proteins 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 101150015201 rbsR gene Proteins 0.000 claims 1
- 230000003248 secreting effect Effects 0.000 claims 1
- 230000028327 secretion Effects 0.000 claims 1
- 229960002180 tetracycline Drugs 0.000 claims 1
- 229930101283 tetracycline Natural products 0.000 claims 1
- 235000019364 tetracycline Nutrition 0.000 claims 1
- 150000003522 tetracyclines Chemical class 0.000 claims 1
- 238000013518 transcription Methods 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
- 101150075472 ycf27 gene Proteins 0.000 claims 1
- 101150032824 ydiV gene Proteins 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261689284P | 2012-05-30 | 2012-05-30 | |
| US61/689,284 | 2012-05-30 | ||
| PCT/US2013/043384 WO2013181404A2 (en) | 2012-05-30 | 2013-05-30 | COMPOSITIONS AND METHODS FOR PEPTIDE EXPRESSION AND PURIFICATION USING A TYPE lll SECRETION SYSTEM |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015523066A JP2015523066A (ja) | 2015-08-13 |
| JP2015523066A5 true JP2015523066A5 (enExample) | 2016-07-21 |
| JP6496243B2 JP6496243B2 (ja) | 2019-04-03 |
Family
ID=49674058
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015515191A Active JP6496243B2 (ja) | 2012-05-30 | 2013-05-30 | Ｉｉｉ型分泌系を使用したペプチド発現および精製のための組成物および方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US9630997B2 (enExample) |
| EP (1) | EP2855676B1 (enExample) |
| JP (1) | JP6496243B2 (enExample) |
| DK (1) | DK2855676T3 (enExample) |
| ES (1) | ES2861275T3 (enExample) |
| PL (1) | PL2855676T3 (enExample) |
| WO (1) | WO2013181404A2 (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013181404A2 (en) * | 2012-05-30 | 2013-12-05 | University Of Utah Research Foundation | COMPOSITIONS AND METHODS FOR PEPTIDE EXPRESSION AND PURIFICATION USING A TYPE lll SECRETION SYSTEM |
| EP3356525A4 (en) | 2015-10-02 | 2019-03-27 | University Of Utah Research Foundation | COMPOSITIONS FOR ADJUSTABLE RIBOSOMTRANSLATION SPEED AND METHOD OF USE |
| US10849938B2 (en) | 2017-09-13 | 2020-12-01 | ZBiotics Company | Gene expression system for probiotic microorganisms |
| CN108837152B (zh) * | 2018-05-23 | 2021-06-22 | 浙江大学 | 鞭毛主调控基因flhDC或其表达产物作为靶点在抗菌药物开发中的应用 |
| JP7609793B2 (ja) * | 2019-03-04 | 2025-01-07 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | バイオコンジュゲーションのために操作されたｃｄ４７の細胞外ドメイン |
| KR20240013956A (ko) * | 2022-07-21 | 2024-01-31 | 충남대학교산학협력단 | 코난토킨-g의 분비를 위한 재조합 발현 벡터 및 이로 형질전환된 약독화 살모넬라 균주 |
| KR20240013955A (ko) * | 2022-07-21 | 2024-01-31 | 충남대학교산학협력단 | 멜리틴 분비를 위한 재조합 발현 벡터 및 이로 형질전환된 약독화 살모넬라 균주 |
| KR20240015035A (ko) * | 2022-07-25 | 2024-02-02 | 충남대학교산학협력단 | 클로로톡신의 분비를 위한 재조합 발현 벡터 및 이로형질전환된 약독화 살모넬라 균주 |
| KR20240015029A (ko) * | 2022-07-25 | 2024-02-02 | 충남대학교산학협력단 | 카탈레이스 분비를 위한 재조합 발현 벡터 및 이로형질전환된 대장균 균주 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7659102B2 (en) * | 2001-02-21 | 2010-02-09 | Verenium Corporation | Amylases, nucleic acids encoding them and methods for making and using them |
| US20040033608A1 (en) * | 2001-10-10 | 2004-02-19 | Purdue Research Foundation | Plasmids, strains, and methods of use |
| AU2002336181A1 (en) * | 2001-10-19 | 2003-04-28 | Paradigm Therapeutics Limited | G-protein coupled receptor |
| US20120027786A1 (en) * | 2010-02-23 | 2012-02-02 | Massachusetts Institute Of Technology | Genetically programmable pathogen sense and destroy |
| WO2013181404A2 (en) * | 2012-05-30 | 2013-12-05 | University Of Utah Research Foundation | COMPOSITIONS AND METHODS FOR PEPTIDE EXPRESSION AND PURIFICATION USING A TYPE lll SECRETION SYSTEM |
-
2013
- 2013-05-30 WO PCT/US2013/043384 patent/WO2013181404A2/en not_active Ceased
- 2013-05-30 JP JP2015515191A patent/JP6496243B2/ja active Active
- 2013-05-30 EP EP13798228.6A patent/EP2855676B1/en active Active
- 2013-05-30 US US14/404,919 patent/US9630997B2/en active Active - Reinstated
- 2013-05-30 ES ES13798228T patent/ES2861275T3/es active Active
- 2013-05-30 DK DK13798228.6T patent/DK2855676T3/da active
- 2013-05-30 PL PL13798228T patent/PL2855676T3/pl unknown
-
2017
- 2017-03-15 US US15/459,191 patent/US10800818B2/en active Active
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