DK2855676T3 - SAMMENSÆTNINGER OG FREMGANGSMÅDER TIL PEPTIDEKSPRESSION OG -OPRENSNING UNDER ANVENDELSE AF ET TYPE lll-SEKRETIONSSYSTEM - Google Patents

SAMMENSÆTNINGER OG FREMGANGSMÅDER TIL PEPTIDEKSPRESSION OG -OPRENSNING UNDER ANVENDELSE AF ET TYPE lll-SEKRETIONSSYSTEM Download PDF

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Publication number
DK2855676T3
DK2855676T3 DK13798228.6T DK13798228T DK2855676T3 DK 2855676 T3 DK2855676 T3 DK 2855676T3 DK 13798228 T DK13798228 T DK 13798228T DK 2855676 T3 DK2855676 T3 DK 2855676T3
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Denmark
Prior art keywords
purification
procedures
compositions
peptide expression
secretary system
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DK13798228.6T
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English (en)
Inventor
Kelly T Hughes
Baldomero M Olivera
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Univ Utah Res Found
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Publication of DK2855676T3 publication Critical patent/DK2855676T3/da

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/255—Salmonella (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43522—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from scorpions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
DK13798228.6T 2012-05-30 2013-05-30 SAMMENSÆTNINGER OG FREMGANGSMÅDER TIL PEPTIDEKSPRESSION OG -OPRENSNING UNDER ANVENDELSE AF ET TYPE lll-SEKRETIONSSYSTEM DK2855676T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261689284P 2012-05-30 2012-05-30
PCT/US2013/043384 WO2013181404A2 (en) 2012-05-30 2013-05-30 COMPOSITIONS AND METHODS FOR PEPTIDE EXPRESSION AND PURIFICATION USING A TYPE lll SECRETION SYSTEM

Publications (1)

Publication Number Publication Date
DK2855676T3 true DK2855676T3 (da) 2021-04-12

Family

ID=49674058

Family Applications (1)

Application Number Title Priority Date Filing Date
DK13798228.6T DK2855676T3 (da) 2012-05-30 2013-05-30 SAMMENSÆTNINGER OG FREMGANGSMÅDER TIL PEPTIDEKSPRESSION OG -OPRENSNING UNDER ANVENDELSE AF ET TYPE lll-SEKRETIONSSYSTEM

Country Status (8)

Country Link
US (2) US9630997B2 (da)
EP (1) EP2855676B1 (da)
JP (1) JP6496243B2 (da)
DK (1) DK2855676T3 (da)
ES (1) ES2861275T3 (da)
HK (1) HK1209158A1 (da)
PL (1) PL2855676T3 (da)
WO (1) WO2013181404A2 (da)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2861275T3 (es) * 2012-05-30 2021-10-06 Univ Utah Res Found Composiciones y métodos para la expresión y la purificación de péptidos usando un sistema de secreción de tipo III
MA43056A (fr) * 2015-10-02 2018-08-08 Univ Utah Res Found Compositions de vitesse de traduction réglable des ribosomes et leurs procédés d'utilisation
US10849938B2 (en) 2017-09-13 2020-12-01 ZBiotics Company Gene expression system for probiotic microorganisms
CN108837152B (zh) * 2018-05-23 2021-06-22 浙江大学 鞭毛主调控基因flhDC或其表达产物作为靶点在抗菌药物开发中的应用
KR20240013955A (ko) * 2022-07-21 2024-01-31 충남대학교산학협력단 멜리틴 분비를 위한 재조합 발현 벡터 및 이로 형질전환된 약독화 살모넬라 균주
KR20240013956A (ko) * 2022-07-21 2024-01-31 충남대학교산학협력단 코난토킨-g의 분비를 위한 재조합 발현 벡터 및 이로 형질전환된 약독화 살모넬라 균주
KR20240015038A (ko) * 2022-07-25 2024-02-02 충남대학교산학협력단 인터루킨-21의 분비를 위한 재조합 발현 벡터 및 이로형질전환된 약독화 살모넬라 균주
KR20240015029A (ko) * 2022-07-25 2024-02-02 충남대학교산학협력단 카탈레이스 분비를 위한 재조합 발현 벡터 및 이로형질전환된 대장균 균주

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7659102B2 (en) * 2001-02-21 2010-02-09 Verenium Corporation Amylases, nucleic acids encoding them and methods for making and using them
US20040033608A1 (en) * 2001-10-10 2004-02-19 Purdue Research Foundation Plasmids, strains, and methods of use
WO2003033536A2 (en) * 2001-10-19 2003-04-24 Paradigm Therapeutics Limited G-protein coupled receptor
US20120027786A1 (en) * 2010-02-23 2012-02-02 Massachusetts Institute Of Technology Genetically programmable pathogen sense and destroy
ES2861275T3 (es) * 2012-05-30 2021-10-06 Univ Utah Res Found Composiciones y métodos para la expresión y la purificación de péptidos usando un sistema de secreción de tipo III

Also Published As

Publication number Publication date
EP2855676B1 (en) 2021-01-13
WO2013181404A2 (en) 2013-12-05
PL2855676T3 (pl) 2021-07-05
WO2013181404A3 (en) 2015-06-04
HK1209158A1 (en) 2016-03-24
US9630997B2 (en) 2017-04-25
EP2855676A4 (en) 2016-04-06
US20180009851A1 (en) 2018-01-11
JP2015523066A (ja) 2015-08-13
US20150225466A1 (en) 2015-08-13
EP2855676A2 (en) 2015-04-08
US10800818B2 (en) 2020-10-13
JP6496243B2 (ja) 2019-04-03
ES2861275T3 (es) 2021-10-06

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