JP2015514212A5 - - Google Patents
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- JP2015514212A5 JP2015514212A5 JP2015503378A JP2015503378A JP2015514212A5 JP 2015514212 A5 JP2015514212 A5 JP 2015514212A5 JP 2015503378 A JP2015503378 A JP 2015503378A JP 2015503378 A JP2015503378 A JP 2015503378A JP 2015514212 A5 JP2015514212 A5 JP 2015514212A5
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- JP
- Japan
- Prior art keywords
- image
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- fluorescence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000000034 method Methods 0.000 claims description 41
- 239000000523 sample Substances 0.000 claims description 36
- 239000003550 marker Substances 0.000 claims description 25
- 239000011230 binding agent Substances 0.000 claims description 20
- 239000002131 composite material Substances 0.000 claims description 18
- 108090000623 proteins and genes Proteins 0.000 claims description 18
- 102000004169 proteins and genes Human genes 0.000 claims description 17
- 239000012472 biological sample Substances 0.000 claims description 16
- 150000007523 nucleic acids Chemical group 0.000 claims description 14
- 238000010186 staining Methods 0.000 claims description 12
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 11
- 230000000877 morphologic effect Effects 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 5
- 230000003321 amplification Effects 0.000 claims description 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 238000001917 fluorescence detection Methods 0.000 claims 3
- 108091005804 Peptidases Proteins 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 238000004043 dyeing Methods 0.000 claims 1
- 238000009396 hybridization Methods 0.000 claims 1
- 238000007850 in situ PCR Methods 0.000 claims 1
- 238000002372 labelling Methods 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 230000003647 oxidation Effects 0.000 claims 1
- 238000007254 oxidation reaction Methods 0.000 claims 1
- 230000037452 priming Effects 0.000 claims 1
- 238000005096 rolling process Methods 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- HSTOKWSFWGCZMH-UHFFFAOYSA-N 3,3'-diaminobenzidine Chemical compound C1=C(N)C(N)=CC=C1C1=CC=C(N)C(N)=C1 HSTOKWSFWGCZMH-UHFFFAOYSA-N 0.000 description 4
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 4
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 4
- 238000002073 fluorescence micrograph Methods 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 3
- 238000003384 imaging method Methods 0.000 description 3
- 238000013507 mapping Methods 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 2
- 210000004940 nucleus Anatomy 0.000 description 2
- 206010009944 Colon cancer Diseases 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 101100239628 Danio rerio myca gene Proteins 0.000 description 1
- 101150029707 ERBB2 gene Proteins 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 101000782132 Homo sapiens Zinc finger protein 217 Proteins 0.000 description 1
- 102000011782 Keratins Human genes 0.000 description 1
- 108010076876 Keratins Proteins 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 102100036595 Zinc finger protein 217 Human genes 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261618132P | 2012-03-30 | 2012-03-30 | |
| US61/618,132 | 2012-03-30 | ||
| PCT/US2013/033249 WO2013148448A1 (en) | 2012-03-30 | 2013-03-21 | Immunoflu0rescence and fluorescent-based nucleic acid analysis on a single sample |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015514212A JP2015514212A (ja) | 2015-05-18 |
| JP2015514212A5 true JP2015514212A5 (https=) | 2017-06-29 |
| JP6225163B2 JP6225163B2 (ja) | 2017-11-01 |
Family
ID=48050953
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015503378A Active JP6225163B2 (ja) | 2012-03-30 | 2013-03-21 | 単一試料での免疫蛍光及び蛍光系核酸分析 |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US20150065371A1 (https=) |
| EP (1) | EP2831591B1 (https=) |
| JP (1) | JP6225163B2 (https=) |
| KR (1) | KR20140142715A (https=) |
| CN (1) | CN104520713A (https=) |
| AU (1) | AU2013240127B2 (https=) |
| CA (1) | CA2867508C (https=) |
| IN (1) | IN2014DN07531A (https=) |
| NZ (1) | NZ630091A (https=) |
| RU (1) | RU2014137129A (https=) |
| SG (1) | SG11201406105UA (https=) |
| WO (1) | WO2013148448A1 (https=) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| EP4148118A1 (en) | 2012-08-24 | 2023-03-15 | Yale University | System, device and method for high-throughput multi-plexed detection |
| US10088658B2 (en) | 2013-03-18 | 2018-10-02 | General Electric Company | Referencing in multi-acquisition slide imaging |
| CA2940115C (en) | 2014-02-24 | 2023-03-14 | Ventana Medical Systems, Inc. | Methods, kits, and systems for scoring the immune response to cancer by simultaneous detection of cd3, cd8, cd20 and foxp3 |
| CN104980558B (zh) * | 2014-04-01 | 2019-01-01 | 中国移动通信集团公司 | 一种信息处理的方法、装置、手机宠物客户端及移动终端 |
| WO2015157246A2 (en) * | 2014-04-07 | 2015-10-15 | Massachusetts Institute Of Technology | Use of microparticle additives to simultaneously enable artifact-free image registration, auto-focusing, and chromatic aberration correction in microscopy |
| WO2016026038A1 (en) * | 2014-08-18 | 2016-02-25 | Viewsiq Inc. | System and method for embedded images in large field-of-view microscopic scans |
| US10584366B2 (en) | 2014-12-03 | 2020-03-10 | IsoPlexis Corporation | Analysis and screening of cell secretion profiles |
| US11353448B2 (en) | 2015-02-13 | 2022-06-07 | California Institute Of Technology | Methods and compositions for quantifying metabolites and proteins from single cells |
| CN107847145B (zh) * | 2015-07-20 | 2020-08-14 | 徐敏 | 光子结构和化学计量学病理系统 |
| EP3396375B1 (en) * | 2015-12-24 | 2021-08-04 | Konica Minolta, Inc. | Image processing device and program |
| CN107419001A (zh) * | 2016-05-23 | 2017-12-01 | 益善生物技术股份有限公司 | Brca1、pten基因异常检测试剂盒及检测方法 |
| JP6231709B1 (ja) * | 2016-05-31 | 2017-11-15 | シスメックス株式会社 | 蛍光画像分析装置および分析方法 |
| WO2017212055A1 (en) * | 2016-06-10 | 2017-12-14 | F. Hoffmann-La Roche Ag | System for bright field image simulation |
| CN106290878A (zh) * | 2016-09-10 | 2017-01-04 | 天津大学 | 基于量子点的胰腺癌肿瘤标志物ca242免疫层析试纸条的制备方法 |
| WO2018049418A1 (en) | 2016-09-12 | 2018-03-15 | IsoPlexis Corporation | System and methods for multiplexed analysis of cellular and other immunotherapeutics |
| CN109791157B (zh) * | 2016-09-26 | 2022-06-07 | 贝克顿迪金森公司 | 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达 |
| EP3538891B1 (en) | 2016-11-11 | 2022-01-05 | Isoplexis Corporation | Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells |
| US11525783B2 (en) | 2016-11-22 | 2022-12-13 | IsoPlexis Corporation | Systems, devices and methods for cell capture and methods of manufacture thereof |
| WO2020039520A1 (ja) * | 2018-08-22 | 2020-02-27 | オリンパス株式会社 | 画像処理装置、撮像システム、画像処理装置の作動方法、及び画像処理装置の作動プログラム |
| US10921223B2 (en) * | 2018-10-12 | 2021-02-16 | Shenzhen Prs Limited | Process record slide for staining and method of using the same |
| CN110498858B (zh) * | 2019-07-26 | 2024-01-23 | 深圳市达科为生物工程有限公司 | 一种动态检测单细胞外泌蛋白分泌情况的方法 |
| US20210182531A1 (en) * | 2019-12-17 | 2021-06-17 | Applied Materials, Inc. | Registration techniques for multiplexed fluorescence in-situ hybridization images |
| EP4147044A4 (en) * | 2020-05-07 | 2024-06-12 | Advanced Cell Diagnostics, Inc. | METHOD FOR THE SIMULTANEOUS DETECTION OF TARGET NUCLEIC ACIDS AND PROTEINS AND KIT THEREFOR |
| EP4182676A1 (en) * | 2020-07-20 | 2023-05-24 | Akoya Biosciences, Inc. | Processing and imaging tissue samples |
| WO2022035943A1 (en) | 2020-08-13 | 2022-02-17 | Ventana Medical Systems, Inc. | Automated identification of necrotic regions in digital images of multiplex immunofluorescence stained tissue |
| WO2022061150A2 (en) * | 2020-09-18 | 2022-03-24 | 10X Geonomics, Inc. | Sample handling apparatus and image registration methods |
| CN112415198B (zh) * | 2020-11-20 | 2022-11-11 | 四川大学华西医院 | Gp1bb检测试剂在制备肺癌筛查试剂盒中的用途 |
| CN113310963B (zh) * | 2021-05-31 | 2023-12-01 | 四川大学华西医院 | 一种改进的中性粒细胞NETs免疫荧光检测方法 |
| AU2022388928A1 (en) | 2021-11-16 | 2024-05-16 | Sotio Biotech Inc. | Treatment of myxoid/round cell liposarcoma patients |
| WO2023147133A1 (en) * | 2022-01-28 | 2023-08-03 | University Of Southern California | Liquid biopsy analytes to define cancer stages |
| CA3243342A1 (en) * | 2022-01-28 | 2023-08-03 | University Of Southern California | IDENTIFICATION OF NON-DISEALED PATIENTS USING DISEASE-ASSOCIATED ASSAY AND ANALYSIS IN LIQUID BIOPSY |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6404906B2 (en) | 1997-03-03 | 2002-06-11 | Bacus Research Laboratories,Inc. | Method and apparatus for acquiring and reconstructing magnified specimen images from a computer-controlled microscope |
| US6993169B2 (en) * | 2001-01-11 | 2006-01-31 | Trestle Corporation | System and method for finding regions of interest for microscopic digital montage imaging |
| US7629125B2 (en) * | 2006-11-16 | 2009-12-08 | General Electric Company | Sequential analysis of biological samples |
| US20110189670A1 (en) * | 2008-07-07 | 2011-08-04 | Ruth L Katz | Circulating Tumor and Tumor Stem Cell Detection Using Genomic Specific Probes |
| US8269827B2 (en) * | 2009-09-29 | 2012-09-18 | General Electric Company | System and methods for mapping fluorescent images into a bright field color space |
-
2013
- 2013-03-21 JP JP2015503378A patent/JP6225163B2/ja active Active
- 2013-03-21 EP EP13715086.8A patent/EP2831591B1/en active Active
- 2013-03-21 CA CA2867508A patent/CA2867508C/en active Active
- 2013-03-21 RU RU2014137129A patent/RU2014137129A/ru not_active Application Discontinuation
- 2013-03-21 CN CN201380017702.4A patent/CN104520713A/zh active Pending
- 2013-03-21 KR KR20147027562A patent/KR20140142715A/ko not_active Withdrawn
- 2013-03-21 NZ NZ630091A patent/NZ630091A/en not_active IP Right Cessation
- 2013-03-21 IN IN7531DEN2014 patent/IN2014DN07531A/en unknown
- 2013-03-21 US US14/387,861 patent/US20150065371A1/en not_active Abandoned
- 2013-03-21 WO PCT/US2013/033249 patent/WO2013148448A1/en not_active Ceased
- 2013-03-21 SG SG11201406105UA patent/SG11201406105UA/en unknown
- 2013-03-21 AU AU2013240127A patent/AU2013240127B2/en active Active
-
2024
- 2024-08-30 US US18/820,303 patent/US20250102507A1/en active Pending
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