JP2015508896A5 - - Google Patents
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- JP2015508896A5 JP2015508896A5 JP2014557852A JP2014557852A JP2015508896A5 JP 2015508896 A5 JP2015508896 A5 JP 2015508896A5 JP 2014557852 A JP2014557852 A JP 2014557852A JP 2014557852 A JP2014557852 A JP 2014557852A JP 2015508896 A5 JP2015508896 A5 JP 2015508896A5
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- 238000000034 method Methods 0.000 claims description 95
- 239000000463 material Substances 0.000 claims description 37
- 108091006146 Channels Proteins 0.000 claims description 22
- 239000000232 Lipid Bilayer Substances 0.000 claims description 17
- 108010013381 Porins Proteins 0.000 claims description 17
- 102000017033 Porins Human genes 0.000 claims description 17
- 150000002632 lipids Chemical class 0.000 claims description 17
- 239000000243 solution Substances 0.000 claims description 17
- 239000007853 buffer solution Substances 0.000 claims description 14
- 230000001939 inductive effect Effects 0.000 claims description 4
- 239000004094 surface-active agent Substances 0.000 claims description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- 230000005661 hydrophobic surface Effects 0.000 claims description 3
- 235000017281 sodium acetate Nutrition 0.000 claims description 3
- 239000001632 sodium acetate Substances 0.000 claims description 3
- 239000000693 micelle Substances 0.000 claims description 2
- 150000007523 nucleic acids Chemical class 0.000 description 35
- 108020004707 nucleic acids Proteins 0.000 description 29
- 102000039446 nucleic acids Human genes 0.000 description 29
- 108091028043 Nucleic acid sequence Proteins 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 101710092462 Alpha-hemolysin Proteins 0.000 description 4
- DIOQZVSQGTUSAI-UHFFFAOYSA-N decane Chemical compound CCCCCCCCCC DIOQZVSQGTUSAI-UHFFFAOYSA-N 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000003960 organic solvent Substances 0.000 description 4
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
- KILNVBDSWZSGLL-KXQOOQHDSA-N 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCCCCCCCCC KILNVBDSWZSGLL-KXQOOQHDSA-N 0.000 description 2
- 108091023037 Aptamer Proteins 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 241000187480 Mycobacterium smegmatis Species 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 229920002477 rna polymer Polymers 0.000 description 2
- 238000000527 sonication Methods 0.000 description 2
- PORPENFLTBBHSG-MGBGTMOVSA-N 1,2-dihexadecanoyl-sn-glycerol-3-phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(O)=O)OC(=O)CCCCCCCCCCCCCCC PORPENFLTBBHSG-MGBGTMOVSA-N 0.000 description 1
- TZCPCKNHXULUIY-RGULYWFUSA-N 1,2-distearoyl-sn-glycero-3-phosphoserine Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(=O)OC[C@H](N)C(O)=O)OC(=O)CCCCCCCCCCCCCCCCC TZCPCKNHXULUIY-RGULYWFUSA-N 0.000 description 1
- ZWZWYGMENQVNFU-UHFFFAOYSA-N Glycerophosphorylserin Natural products OC(=O)C(N)COP(O)(=O)OCC(O)CO ZWZWYGMENQVNFU-UHFFFAOYSA-N 0.000 description 1
- JCABVIFDXFFRMT-DIPNUNPCSA-N [(2r)-1-[ethoxy(hydroxy)phosphoryl]oxy-3-hexadecanoyloxypropan-2-yl] octadec-9-enoate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(=O)OCC)OC(=O)CCCCCCCC=CCCCCCCCC JCABVIFDXFFRMT-DIPNUNPCSA-N 0.000 description 1
- ATBOMIWRCZXYSZ-XZBBILGWSA-N [1-[2,3-dihydroxypropoxy(hydroxy)phosphoryl]oxy-3-hexadecanoyloxypropan-2-yl] (9e,12e)-octadeca-9,12-dienoate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(COP(O)(=O)OCC(O)CO)OC(=O)CCCCCCC\C=C\C\C=C\CCCCC ATBOMIWRCZXYSZ-XZBBILGWSA-N 0.000 description 1
- AWUCVROLDVIAJX-UHFFFAOYSA-N alpha-glycerophosphate Natural products OCC(O)COP(O)(O)=O AWUCVROLDVIAJX-UHFFFAOYSA-N 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000009499 grossing Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 150000003905 phosphatidylinositols Chemical class 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261599871P | 2012-02-16 | 2012-02-16 | |
| US61/599,871 | 2012-02-16 | ||
| US201261600398P | 2012-02-17 | 2012-02-17 | |
| US61/600,398 | 2012-02-17 | ||
| PCT/US2013/026514 WO2013123450A1 (en) | 2012-02-16 | 2013-02-15 | Methods for creating bilayers for use with nanopore sensors |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017049533A Division JP6431108B2 (ja) | 2012-02-16 | 2017-03-15 | 標的分子を検出する方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015508896A JP2015508896A (ja) | 2015-03-23 |
| JP2015508896A5 true JP2015508896A5 (https=) | 2016-01-28 |
| JP6178805B2 JP6178805B2 (ja) | 2017-08-09 |
Family
ID=48984792
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014557852A Active JP6178805B2 (ja) | 2012-02-16 | 2013-02-15 | ナノ細孔センサーとともに使用するための二重層を作製するための方法 |
| JP2017049533A Active JP6431108B2 (ja) | 2012-02-16 | 2017-03-15 | 標的分子を検出する方法 |
| JP2018034689A Active JP6617167B2 (ja) | 2012-02-16 | 2018-02-28 | ナノ細孔センサーとともに使用するための二重層を作製するための方法 |
| JP2019094447A Withdrawn JP2019164155A (ja) | 2012-02-16 | 2019-05-20 | ナノ細孔センサーとともに使用するための二重層を作製するための方法 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017049533A Active JP6431108B2 (ja) | 2012-02-16 | 2017-03-15 | 標的分子を検出する方法 |
| JP2018034689A Active JP6617167B2 (ja) | 2012-02-16 | 2018-02-28 | ナノ細孔センサーとともに使用するための二重層を作製するための方法 |
| JP2019094447A Withdrawn JP2019164155A (ja) | 2012-02-16 | 2019-05-20 | ナノ細孔センサーとともに使用するための二重層を作製するための方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (5) | US9850534B2 (https=) |
| EP (2) | EP3540077B1 (https=) |
| JP (4) | JP6178805B2 (https=) |
| CN (2) | CN104254619B (https=) |
| CA (1) | CA2864125C (https=) |
| ES (1) | ES2732256T3 (https=) |
| WO (1) | WO2013123450A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11041198B2 (en) | 2016-03-30 | 2021-06-22 | Roche Sequencing Solutions, Inc. | Electrical enhancement of bilayer formation |
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| WO2012083249A2 (en) | 2010-12-17 | 2012-06-21 | The Trustees Of Columbia University In The City Of New York | Dna sequencing by synthesis using modified nucleotides and nanopore detection |
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| CN104254619B (zh) | 2012-02-16 | 2018-08-24 | 吉尼亚科技公司 | 产生用于纳米孔传感器的双层的方法 |
| WO2013154999A2 (en) | 2012-04-09 | 2013-10-17 | The Trustees Of Columbia University In The City Of New York | Method of preparation of nanopore and uses thereof |
| WO2013188841A1 (en) * | 2012-06-15 | 2013-12-19 | Genia Technologies, Inc. | Chip set-up and high-accuracy nucleic acid sequencing |
| EP3674412A1 (en) * | 2012-06-20 | 2020-07-01 | The Trustees of Columbia University in the City of New York | Nucleic acid sequencing by nanopore detection of tag molecules |
| WO2014144883A1 (en) | 2013-03-15 | 2014-09-18 | The Trustees Of Columbia University In The City Of New York | Raman cluster tagged molecules for biological imaging |
| US10732183B2 (en) | 2013-03-15 | 2020-08-04 | The Trustees Of Columbia University In The City Of New York | Method for detecting multiple predetermined compounds in a sample |
| KR102245192B1 (ko) | 2013-05-06 | 2021-04-29 | 온테라 인크. | 나노포어를 이용한 표적 검출 |
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| WO2014190299A2 (en) * | 2013-05-23 | 2014-11-27 | Arizona Board Of Regents Acting For And On Behalf Of Arizona State University | Chemistry, systems and methods of translocation of a polymer through a nanopore |
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2013
- 2013-02-15 CN CN201380015148.6A patent/CN104254619B/zh active Active
- 2013-02-15 CA CA2864125A patent/CA2864125C/en active Active
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- 2013-02-15 CN CN201810865708.1A patent/CN108949944A/zh active Pending
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11041198B2 (en) | 2016-03-30 | 2021-06-22 | Roche Sequencing Solutions, Inc. | Electrical enhancement of bilayer formation |
| US11814676B2 (en) | 2016-03-30 | 2023-11-14 | Roche Sequencing Solutions, Inc. | Electrical enhancement of bilayer formation |
| US12173367B2 (en) | 2016-03-30 | 2024-12-24 | Roche Sequencing Solutions, Inc. | Electrical enhancement of bilayer formation |
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