JP2015508705A5 - - Google Patents
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- JP2015508705A5 JP2015508705A5 JP2014556753A JP2014556753A JP2015508705A5 JP 2015508705 A5 JP2015508705 A5 JP 2015508705A5 JP 2014556753 A JP2014556753 A JP 2014556753A JP 2014556753 A JP2014556753 A JP 2014556753A JP 2015508705 A5 JP2015508705 A5 JP 2015508705A5
- Authority
- JP
- Japan
- Prior art keywords
- fluid
- fluid line
- pulses emitted
- pulsed laser
- opening
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000012530 fluid Substances 0.000 claims 98
- 238000000034 method Methods 0.000 claims 25
- 239000012528 membrane Substances 0.000 claims 8
- 230000015572 biosynthetic process Effects 0.000 claims 4
- 238000012544 monitoring process Methods 0.000 claims 4
- 238000004891 communication Methods 0.000 claims 2
- 230000005489 elastic deformation Effects 0.000 claims 2
- 230000001678 irradiating effect Effects 0.000 claims 2
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 claims 1
- 239000002245 particle Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/370,196 | 2012-02-09 | ||
| US13/370,196 US9176504B2 (en) | 2011-02-11 | 2012-02-09 | High-speed on demand droplet generation and single cell encapsulation driven by induced cavitation |
| PCT/US2013/025434 WO2013120016A2 (en) | 2012-02-09 | 2013-02-08 | High-speed on demand droplet generation and single cell encapsulation driven by induced cavitation |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017172951A Division JP2018034154A (ja) | 2012-02-09 | 2017-09-08 | 誘発されたキャビテーションによって駆動される高速オンデマンド型液滴生成および単一細胞封入 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015508705A JP2015508705A (ja) | 2015-03-23 |
| JP2015508705A5 true JP2015508705A5 (enExample) | 2016-03-31 |
| JP6367717B2 JP6367717B2 (ja) | 2018-08-01 |
Family
ID=50686259
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014556753A Expired - Fee Related JP6367717B2 (ja) | 2012-02-09 | 2013-02-08 | 誘発されたキャビテーションによって駆動される高速オンデマンド型液滴生成および単一細胞封入 |
| JP2017172951A Pending JP2018034154A (ja) | 2012-02-09 | 2017-09-08 | 誘発されたキャビテーションによって駆動される高速オンデマンド型液滴生成および単一細胞封入 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017172951A Pending JP2018034154A (ja) | 2012-02-09 | 2017-09-08 | 誘発されたキャビテーションによって駆動される高速オンデマンド型液滴生成および単一細胞封入 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US9176504B2 (enExample) |
| EP (2) | EP3410124B1 (enExample) |
| JP (2) | JP6367717B2 (enExample) |
| KR (1) | KR20150016929A (enExample) |
| CN (2) | CN104321652B (enExample) |
| AU (2) | AU2013216804B2 (enExample) |
| CA (1) | CA2864138C (enExample) |
| ES (2) | ES2687684T3 (enExample) |
| WO (1) | WO2013120016A2 (enExample) |
Families Citing this family (68)
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|---|---|---|---|---|
| US9364831B2 (en) | 2009-08-08 | 2016-06-14 | The Regents Of The University Of California | Pulsed laser triggered high speed microfluidic switch and applications in fluorescent activated cell sorting |
| US9176504B2 (en) | 2011-02-11 | 2015-11-03 | The Regents Of The University Of California | High-speed on demand droplet generation and single cell encapsulation driven by induced cavitation |
| WO2013177560A1 (en) | 2012-05-25 | 2013-11-28 | The Regents Of The University Of California | Microfluidic systems for particle trapping and separation |
| US9103468B2 (en) * | 2012-07-30 | 2015-08-11 | University Of South Carolina | Enhanced flow boiling in microchannels by high frequency microbubble-excited and -modulated oscillations |
| US9731293B2 (en) * | 2012-10-03 | 2017-08-15 | The United States Of America, As Represented By The Secretary Of The Navy | Paired laser and electrokinetic separation, manipulation, and analysis device |
| US20140151229A1 (en) | 2012-12-05 | 2014-06-05 | Caliper Life Sciences, Inc. | Manipulation of objects in microfluidic devices using external electrodes |
| US9297784B2 (en) * | 2012-12-05 | 2016-03-29 | Caliper Life Sciences, Inc. | Device and method for extracting target objects from a sample |
| US9995412B2 (en) * | 2013-03-01 | 2018-06-12 | Wave 80 Biosciences, Inc. | Long-throw microfluidic actuator |
| US10071359B2 (en) | 2013-03-15 | 2018-09-11 | The Regents Of The University Of California | High-speed on demand microfluidic droplet generation and manipulation |
| EP2848698A1 (en) * | 2013-08-26 | 2015-03-18 | F. Hoffmann-La Roche AG | System and method for automated nucleic acid amplification |
| CN103614290B (zh) * | 2013-11-11 | 2015-03-04 | 北京工业大学 | 面向荧光pcr微系统的往复式循环单微通道装置 |
| US10960396B2 (en) | 2014-05-16 | 2021-03-30 | Cytonome/St, Llc | Thermal activated microfluidic switching |
| CA2890401C (en) | 2015-01-21 | 2015-11-03 | Vln Advanced Technologies Inc. | Electrodischarge apparatus for generating low-frequency powerful pulsed and cavitating waterjets |
| CA2984492A1 (en) | 2015-04-29 | 2016-11-03 | Flodesign Sonics, Inc. | Acoustophoretic device for angled wave particle deflection |
| CN104941702B (zh) * | 2015-05-25 | 2017-01-04 | 重庆大学 | 一种微通道内产生超小液滴的方法 |
| WO2017004676A1 (en) * | 2015-07-09 | 2017-01-12 | Monash University | On-demand single cell encapsulation using a localised acoustic field |
| US9862941B2 (en) | 2015-10-14 | 2018-01-09 | Pioneer Hi-Bred International, Inc. | Single cell microfluidic device |
| CA2921675C (en) | 2016-02-24 | 2017-12-05 | Vln Advanced Technologies Inc. | Electro-discharge system for neutralizing landmines |
| FR3049348B1 (fr) * | 2016-03-23 | 2023-08-11 | Commissariat Energie Atomique | Procede de caracterisation d’une particule dans un echantillon |
| US12345708B2 (en) * | 2016-03-25 | 2025-07-01 | President And Fellows Of Harvard College | Microfluidic determination of immune and other cells |
| US10228317B1 (en) * | 2016-08-25 | 2019-03-12 | Verily Life Sciences Llc | Multiplexed microfluidic cell sorting using laser induced cavitation bubbles |
| CN106190779B (zh) * | 2016-08-29 | 2018-08-24 | 苏州汶颢芯片科技有限公司 | 基于微流控芯片的单细胞分离和包封装置和方法 |
| WO2018071448A1 (en) | 2016-10-11 | 2018-04-19 | The Regents Of The University Of California | Systems and methods to encapsulate and preserve organic matter for analysis |
| WO2018075069A1 (en) * | 2016-10-21 | 2018-04-26 | Hewlett-Packard Development Company, L.P. | Droplet generator |
| US10718004B2 (en) | 2016-12-20 | 2020-07-21 | Lawrence Livermore National Security, Llc | Droplet array for single-cell analysis |
| EP3558524A1 (en) * | 2016-12-22 | 2019-10-30 | IMEC vzw | A microfluidic device for sorting out droplets |
| CN106890683B (zh) * | 2017-03-14 | 2019-03-08 | 广东工业大学 | 一种多层液滴包裹的形成方法 |
| US10544413B2 (en) | 2017-05-18 | 2020-01-28 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| EP4435113B1 (en) | 2017-05-18 | 2025-12-10 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| WO2018227204A1 (en) * | 2017-06-09 | 2018-12-13 | The Regents Of The University Of California | Controlled encapsulation in droplets by liquid-liquid interfacial shearing |
| US11517901B2 (en) | 2017-06-09 | 2022-12-06 | The Regents Of The University Of California | High-efficiency particle encapsulation in droplets with particle spacing and downstream droplet sorting |
| US10821442B2 (en) | 2017-08-22 | 2020-11-03 | 10X Genomics, Inc. | Devices, systems, and kits for forming droplets |
| WO2019075409A1 (en) | 2017-10-12 | 2019-04-18 | The Regents Of The University Of California | ISOLATION AND IDENTIFICATION WITHOUT MICROFLUIDIC LABEL OF CELLS USING FLUORESCENCE LIFE IMAGING (FLIM) |
| US11499127B2 (en) | 2017-10-20 | 2022-11-15 | The Regents Of The University Of California | Multi-layered microfluidic systems for in vitro large-scale perfused capillary networks |
| WO2019079787A1 (en) | 2017-10-20 | 2019-04-25 | The Regents Of The University Of California | MICROFLUIDIC SYSTEMS AND METHODS FOR CELL TRANSFECTION VIA LIPOFLEX |
| WO2019083852A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING |
| CN108837778B (zh) * | 2018-06-05 | 2020-09-15 | 中南大学 | 一种制备核壳结构载药纳米粒的方法 |
| CN110819523B (zh) * | 2018-08-13 | 2023-08-29 | 上海新微技术研发中心有限公司 | 一种数字pcr系统及数字pcr液滴形成方法 |
| EP3613498A1 (en) * | 2018-08-24 | 2020-02-26 | Université de Liège | Microfluidic module for co-encapsulation in droplets |
| CN109051819A (zh) * | 2018-08-27 | 2018-12-21 | 江苏大学 | 一种利用空泡输运颗粒的装置与方法 |
| CN108993342B (zh) * | 2018-09-06 | 2024-01-23 | 山东清创化工有限公司 | 一种管式反应器内光化学反应的微混合系统及方法 |
| CN109269762B (zh) * | 2018-10-16 | 2020-04-28 | 四川大学 | 利用同轴平行光源观测激光空泡冲击波的实验装置 |
| WO2020176882A1 (en) | 2019-02-28 | 2020-09-03 | 10X Genomics, Inc. | Devices, systems, and methods for increasing droplet formation efficiency |
| CN110346074A (zh) * | 2019-06-13 | 2019-10-18 | 江苏大学 | 一种测量激光诱导空泡溃灭冲击力的装置及方法 |
| US12186751B2 (en) | 2019-06-28 | 2025-01-07 | 10X Genomics, Inc. | Devices and systems incorporating acoustic ordering and methods of use thereof |
| US12179199B2 (en) | 2019-08-09 | 2024-12-31 | The Regents Of The University Of California | Microfluidic single-cell pairing array for studying cell-cell interactions in isolated compartments |
| CN110743635A (zh) * | 2019-10-27 | 2020-02-04 | 苏州济研生物医药科技有限公司 | 一种基于负压且无需流体剪切力的液滴生成装置 |
| US12059679B2 (en) | 2019-11-19 | 2024-08-13 | 10X Genomics, Inc. | Methods and devices for sorting droplets and particles |
| CN111077487A (zh) * | 2019-12-03 | 2020-04-28 | 中广核工程有限公司 | 便携式核仪表系统信号检测及故障定位装置 |
| WO2021162700A1 (en) * | 2020-02-14 | 2021-08-19 | Hewlett-Packard Development Company, L.P. | Droplet delivery |
| CN111235029B (zh) * | 2020-03-09 | 2023-01-31 | 辽宁中医药大学 | 一种多功能微流控芯片及其制备方法与应用 |
| CN111495450B (zh) * | 2020-04-24 | 2021-04-06 | 清华大学 | 基于柱塞-叠片混合流的液-液-液三相流微流体芯片 |
| JP7635212B2 (ja) * | 2020-04-30 | 2025-02-25 | 株式会社堀場製作所 | 粒子径分布測定装置、粒子分析ユニット、粒子径分布測定装置用プログラム、及び泡抜き方法 |
| JP7737720B2 (ja) * | 2020-06-16 | 2025-09-11 | 国立研究開発法人理化学研究所 | ラマン散乱分光測定装置およびラマン散乱分光法 |
| KR102491087B1 (ko) * | 2020-10-12 | 2023-01-19 | 인제대학교 산학협력단 | 정전용량형 전극을 이용한 액적 길이 측정장치 |
| US20240085288A1 (en) | 2021-01-11 | 2024-03-14 | Vib Vzw | Means and methods for time-resolved sampling |
| US20220280941A1 (en) * | 2021-03-05 | 2022-09-08 | Enumerix, Inc. | Systems and methods for generating droplets and performing digital analyses |
| CN117241878A (zh) | 2021-03-05 | 2023-12-15 | 伊努梅里斯公司 | 用于生成微滴和执行数字分析的系统和方法 |
| WO2022256612A1 (en) * | 2021-06-04 | 2022-12-08 | Enumerix, Inc. | Compositions, methods, and systems for single cell barcoding and sequencing |
| EP4351788A4 (en) | 2021-06-04 | 2025-04-09 | Enumerix, Inc. | COMPOSITIONS, METHODS AND SYSTEMS FOR SINGLE-CELL ENCODING AND SEQUENCING |
| CN113654763A (zh) * | 2021-09-16 | 2021-11-16 | 中国科学院力学研究所 | 一种基于多种传感器的近壁面射流压力与速度测量的实验系统 |
| GB202113798D0 (en) | 2021-09-27 | 2021-11-10 | Univ Tartu | Microfluidics method |
| CN113967487B (zh) * | 2021-10-11 | 2022-12-02 | 华中科技大学 | 一种喷嘴、液滴光热操控系统及其应用 |
| US20230339226A1 (en) * | 2022-04-22 | 2023-10-26 | Bwxt Nog Technologies, Inc. | Drop-on-demand electroprinter with a plunging wire-in-a-nozzle |
| CN115364779A (zh) * | 2022-07-14 | 2022-11-22 | 南京信息工程大学 | 一种用于激光诱导击穿光谱的液态气溶胶发生装置 |
| CN115364916B (zh) * | 2022-08-19 | 2025-07-25 | 栎真生物科技(上海)有限公司 | 一种微小反应单元的生成装置及生成方法 |
| CN115554954A (zh) * | 2022-10-14 | 2023-01-03 | 中国计量大学 | 一种激光诱导空化溃灭驱动的气泡内液滴封装方法 |
| JP2025535563A (ja) | 2022-11-07 | 2025-10-24 | シェンノン バイオテクノロジーズ インコーポレイテッド | 細胞－細胞相互作用のハイスループットスクリーニングのためのマイクロ流体デバイス |
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| US9364831B2 (en) | 2009-08-08 | 2016-06-14 | The Regents Of The University Of California | Pulsed laser triggered high speed microfluidic switch and applications in fluorescent activated cell sorting |
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| US20110114190A1 (en) * | 2009-11-16 | 2011-05-19 | The Hong Kong University Of Science And Technology | Microfluidic droplet generation and/or manipulation with electrorheological fluid |
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| BR112012020285B1 (pt) * | 2010-02-12 | 2020-12-29 | Dan Angelescu | dispositivo, ferramenta, sistema e método para comunicar sua posição por emissão acústica |
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| US9176504B2 (en) | 2011-02-11 | 2015-11-03 | The Regents Of The University Of California | High-speed on demand droplet generation and single cell encapsulation driven by induced cavitation |
| US10071359B2 (en) | 2013-03-15 | 2018-09-11 | The Regents Of The University Of California | High-speed on demand microfluidic droplet generation and manipulation |
-
2012
- 2012-02-09 US US13/370,196 patent/US9176504B2/en active Active
-
2013
- 2013-02-08 EP EP18178872.0A patent/EP3410124B1/en active Active
- 2013-02-08 JP JP2014556753A patent/JP6367717B2/ja not_active Expired - Fee Related
- 2013-02-08 EP EP13746888.0A patent/EP2812709B1/en active Active
- 2013-02-08 CA CA2864138A patent/CA2864138C/en active Active
- 2013-02-08 KR KR1020147025295A patent/KR20150016929A/ko not_active Ceased
- 2013-02-08 ES ES13746888.0T patent/ES2687684T3/es active Active
- 2013-02-08 CN CN201380016299.3A patent/CN104321652B/zh active Active
- 2013-02-08 ES ES18178872T patent/ES2832486T3/es active Active
- 2013-02-08 WO PCT/US2013/025434 patent/WO2013120016A2/en not_active Ceased
- 2013-02-08 CN CN201710078940.6A patent/CN106732223B/zh active Active
- 2013-02-08 AU AU2013216804A patent/AU2013216804B2/en not_active Ceased
-
2015
- 2015-11-02 US US14/930,054 patent/US10232368B2/en active Active
-
2016
- 2016-09-30 AU AU2016234996A patent/AU2016234996B2/en not_active Ceased
-
2017
- 2017-09-08 JP JP2017172951A patent/JP2018034154A/ja active Pending
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