JP2015139434A5 - - Google Patents
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- Publication number
- JP2015139434A5 JP2015139434A5 JP2014015768A JP2014015768A JP2015139434A5 JP 2015139434 A5 JP2015139434 A5 JP 2015139434A5 JP 2014015768 A JP2014015768 A JP 2014015768A JP 2014015768 A JP2014015768 A JP 2014015768A JP 2015139434 A5 JP2015139434 A5 JP 2015139434A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- cells
- microorganism
- test sample
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 244000005700 microbiome Species 0.000 claims description 43
- 150000007523 nucleic acids Chemical class 0.000 claims description 42
- 108020004707 nucleic acids Proteins 0.000 claims description 41
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 39
- 230000003321 amplification Effects 0.000 claims description 16
- 239000006285 cell suspension Substances 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 11
- 238000006073 displacement reaction Methods 0.000 claims description 10
- 239000003814 drug Substances 0.000 claims description 9
- 229940079593 drugs Drugs 0.000 claims description 9
- 230000035699 permeability Effects 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- KWIUHFFTVRNATP-UHFFFAOYSA-N Trimethylglycine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims description 6
- 239000003153 chemical reaction reagent Substances 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 6
- 239000012138 yeast extract Substances 0.000 claims description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 3
- 229960003237 betaine Drugs 0.000 claims description 3
- 239000004094 surface-active agent Substances 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 2
- 150000002632 lipids Chemical class 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 235000000346 sugar Nutrition 0.000 claims description 2
- 150000008163 sugars Chemical class 0.000 claims description 2
- 210000004027 cells Anatomy 0.000 description 73
- 239000000523 sample Substances 0.000 description 18
- 102100006037 MUC1 Human genes 0.000 description 12
- 241000589248 Legionella Species 0.000 description 6
- 208000007764 Legionnaires' Disease Diseases 0.000 description 6
- 229920003013 deoxyribonucleic acid Polymers 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 239000000725 suspension Substances 0.000 description 4
- 229920000160 (ribonucleotides)n+m Polymers 0.000 description 3
- 238000007397 LAMP assay Methods 0.000 description 3
- 241000589242 Legionella pneumophila Species 0.000 description 3
- 210000003097 Mucus Anatomy 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000002504 physiological saline solution Substances 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000002194 synthesizing Effects 0.000 description 3
- 229940041514 Candida albicans extract Drugs 0.000 description 2
- 210000000170 Cell Membrane Anatomy 0.000 description 2
- 210000002421 Cell Wall Anatomy 0.000 description 2
- 229920002676 Complementary DNA Polymers 0.000 description 2
- 108020004635 Complementary DNA Proteins 0.000 description 2
- 101700011961 DPOM Proteins 0.000 description 2
- 108010092799 EC 2.7.7.49 Proteins 0.000 description 2
- 102000033147 ERVK-25 Human genes 0.000 description 2
- 101710029649 MDV043 Proteins 0.000 description 2
- 101700061424 POLB Proteins 0.000 description 2
- 101700054624 RF1 Proteins 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 238000010804 cDNA synthesis Methods 0.000 description 2
- 230000000295 complement Effects 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 210000004877 mucosa Anatomy 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- IFQSXNOEEPCSLW-DKWTVANSSA-N (2R)-2-amino-3-sulfanylpropanoic acid;hydron;chloride Chemical compound Cl.SC[C@H](N)C(O)=O IFQSXNOEEPCSLW-DKWTVANSSA-N 0.000 description 1
- 108020004465 16S Ribosomal RNA Proteins 0.000 description 1
- KPGXRSRHYNQIFN-UHFFFAOYSA-L 2-oxoglutarate(2-) Chemical compound [O-]C(=O)CCC(=O)C([O-])=O KPGXRSRHYNQIFN-UHFFFAOYSA-L 0.000 description 1
- 239000007991 ACES buffer Substances 0.000 description 1
- 229940064005 Antibiotic throat preparations Drugs 0.000 description 1
- 229940083879 Antibiotics FOR TREATMENT OF HEMORRHOIDS AND ANAL FISSURES FOR TOPICAL USE Drugs 0.000 description 1
- 229940042052 Antibiotics for systemic use Drugs 0.000 description 1
- 229940042786 Antitubercular Antibiotics Drugs 0.000 description 1
- 210000004369 Blood Anatomy 0.000 description 1
- DEGAKNSWVGKMLS-UHFFFAOYSA-N Calcein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC(CN(CC(O)=O)CC(O)=O)=C(O)C=C1OC1=C2C=C(CN(CC(O)=O)CC(=O)O)C(O)=C1 DEGAKNSWVGKMLS-UHFFFAOYSA-N 0.000 description 1
- 210000000795 Conjunctiva Anatomy 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 229940093922 Gynecological Antibiotics Drugs 0.000 description 1
- 229940115932 Legionella pneumophila Drugs 0.000 description 1
- 210000004251 Milk, Human Anatomy 0.000 description 1
- 210000000214 Mouth Anatomy 0.000 description 1
- 229920001850 Nucleic acid sequence Polymers 0.000 description 1
- 229960002378 Oftasceine Drugs 0.000 description 1
- 210000002826 Placenta Anatomy 0.000 description 1
- 208000002151 Pleural Effusion Diseases 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 210000004915 Pus Anatomy 0.000 description 1
- 210000001533 Respiratory Mucosa Anatomy 0.000 description 1
- 210000003296 Saliva Anatomy 0.000 description 1
- 210000003491 Skin Anatomy 0.000 description 1
- 210000003802 Sputum Anatomy 0.000 description 1
- 210000001179 Synovial Fluid Anatomy 0.000 description 1
- 229940024982 Topical Antifungal Antibiotics Drugs 0.000 description 1
- 210000002700 Urine Anatomy 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000003115 biocidal Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- UIIMBOGNXHQVGW-UHFFFAOYSA-M buffer Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 1
- 239000012459 cleaning agent Substances 0.000 description 1
- 230000000875 corresponding Effects 0.000 description 1
- 230000014670 detection of bacterium Effects 0.000 description 1
- 230000001079 digestive Effects 0.000 description 1
- 230000001747 exhibiting Effects 0.000 description 1
- 239000011706 ferric diphosphate Substances 0.000 description 1
- 235000007144 ferric diphosphate Nutrition 0.000 description 1
- 229940036404 ferric pyrophosphate Drugs 0.000 description 1
- 230000002496 gastric Effects 0.000 description 1
- 230000002068 genetic Effects 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 239000008235 industrial water Substances 0.000 description 1
- 229940079866 intestinal antibiotics Drugs 0.000 description 1
- 229940005935 ophthalmologic Antibiotics Drugs 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- -1 wiped samples Substances 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014015768A JP6139425B2 (ja) | 2014-01-30 | 2014-01-30 | 微生物検出法及び微生物検出キット |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014015768A JP6139425B2 (ja) | 2014-01-30 | 2014-01-30 | 微生物検出法及び微生物検出キット |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015139434A JP2015139434A (ja) | 2015-08-03 |
| JP2015139434A5 true JP2015139434A5 (fr) | 2016-02-18 |
| JP6139425B2 JP6139425B2 (ja) | 2017-05-31 |
Family
ID=53770242
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014015768A Active JP6139425B2 (ja) | 2014-01-30 | 2014-01-30 | 微生物検出法及び微生物検出キット |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP6139425B2 (fr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017010001A1 (fr) * | 2015-07-16 | 2017-01-19 | 森永乳業株式会社 | Procédé de détection de micro-organismes et kit de détection de micro-organismes |
| WO2017009999A1 (fr) * | 2015-07-16 | 2017-01-19 | 森永乳業株式会社 | Procédé de détection de micro-organismes et kit de détection de micro-organismes |
| JP6584986B2 (ja) | 2016-03-18 | 2019-10-02 | 株式会社東芝 | 核酸検出方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102471768B (zh) * | 2009-07-24 | 2014-07-23 | 森永乳业株式会社 | 用于微生物检测的方法及试剂盒 |
-
2014
- 2014-01-30 JP JP2014015768A patent/JP6139425B2/ja active Active
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