JP2014519832A5 - - Google Patents
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- JP2014519832A5 JP2014519832A5 JP2014516354A JP2014516354A JP2014519832A5 JP 2014519832 A5 JP2014519832 A5 JP 2014519832A5 JP 2014516354 A JP2014516354 A JP 2014516354A JP 2014516354 A JP2014516354 A JP 2014516354A JP 2014519832 A5 JP2014519832 A5 JP 2014519832A5
- Authority
- JP
- Japan
- Prior art keywords
- range
- chir
- cells
- concentration
- activin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 12
- 241000766026 Coregonus nasus Species 0.000 claims 9
- 210000004039 endoderm cell Anatomy 0.000 claims 6
- 108010023082 activin A Proteins 0.000 claims 4
- 210000000130 stem cell Anatomy 0.000 claims 4
- 210000001671 embryonic stem cell Anatomy 0.000 claims 3
- 238000011534 incubation Methods 0.000 claims 3
- 239000002609 medium Substances 0.000 claims 3
- 239000003814 drug Substances 0.000 claims 2
- 229940079593 drug Drugs 0.000 claims 2
- 239000001963 growth medium Substances 0.000 claims 2
- 230000000638 stimulation Effects 0.000 claims 2
- 239000012980 RPMI-1640 medium Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 210000001900 endoderm Anatomy 0.000 claims 1
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11170713.9 | 2011-06-21 | ||
| EP11170713 | 2011-06-21 | ||
| US201161501351P | 2011-06-27 | 2011-06-27 | |
| US61/501,351 | 2011-06-27 | ||
| PCT/EP2012/062013 WO2012175633A1 (en) | 2011-06-21 | 2012-06-21 | Efficient induction of definitive endoderm from pluripotent stem cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014519832A JP2014519832A (ja) | 2014-08-21 |
| JP2014519832A5 true JP2014519832A5 (cg-RX-API-DMAC7.html) | 2015-08-06 |
| JP6312591B2 JP6312591B2 (ja) | 2018-04-18 |
Family
ID=47422055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014516354A Active JP6312591B2 (ja) | 2011-06-21 | 2012-06-21 | 多能性幹細胞からの胚体内胚葉の効率的な誘導 |
Country Status (6)
| Country | Link |
|---|---|
| US (3) | US20140234963A1 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2723852B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6312591B2 (cg-RX-API-DMAC7.html) |
| CN (2) | CN103890167A (cg-RX-API-DMAC7.html) |
| ES (1) | ES2902650T3 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2012175633A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140234963A1 (en) | 2011-06-21 | 2014-08-21 | Novo Nordisk A/S | Efficient induction of definitive endoderm from pluripotent stem cells |
| WO2014033322A1 (en) | 2012-09-03 | 2014-03-06 | Novo Nordisk A/S | Generation of pancreatic endoderm from pluripotent stem cells using small molecules |
| GB201317869D0 (en) | 2013-10-09 | 2013-11-20 | Cambridge Entpr Ltd | In vitro production of foregut stem cells |
| MA45747A (fr) | 2016-02-24 | 2019-01-02 | Novo Nordisk As | Génération de cellules bêta fonctionnelles à partir de progéniteurs endocrines dérivés de cellules souhes pluripotentes humaines |
| US20190071645A1 (en) * | 2017-09-07 | 2019-03-07 | Sergio Mora | Efficient differentiation of human stem cells to definitive endoderm |
| AU2018330499B2 (en) | 2017-09-11 | 2025-01-30 | Novo Nordisk A/S | Enrichment of NKX6.1 and C-peptide co-expressing cells derived in vitro from stem cells |
| US12421499B2 (en) | 2018-05-11 | 2025-09-23 | Cornell University | Method of differentiation of human pluripotent stem cells to monohormonal cells |
| AU2018439389A1 (en) | 2018-08-30 | 2021-02-18 | Novo Nordisk A/S | Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors |
| EP3953451A1 (en) | 2019-04-08 | 2022-02-16 | Novo Nordisk A/S | Generation of pancreatic endoderm from stem cell derived definitive endoderm |
| JP2023503302A (ja) | 2019-11-22 | 2023-01-27 | ノヴォ ノルディスク アー/エス | スピン凝集した神経系マイクロスフェアおよびその応用 |
| CN116018150A (zh) | 2020-08-28 | 2023-04-25 | 诺和诺德股份有限公司 | 用于筛选干细胞衍生的β样细胞的体外群体的方法及其新型标志物 |
| CN119546749A (zh) | 2022-07-06 | 2025-02-28 | 诺和诺德股份有限公司 | 干细胞向胰腺内分泌细胞的分化 |
| WO2024151541A1 (en) | 2023-01-09 | 2024-07-18 | Sana Biotechnology, Inc. | Type-1 diabetes autoimmune mouse |
| WO2024243236A2 (en) | 2023-05-22 | 2024-11-28 | Sana Biotechnology, Inc. | Methods of delivery of islet cells and related methods |
| WO2025093467A1 (en) | 2023-10-31 | 2025-05-08 | Novo Nordisk A/S | Methods for preventing unwanted cells arising during differentiation of human pluripotent stem cells |
| EP4491712A1 (en) | 2024-03-20 | 2025-01-15 | Novo Nordisk A/S | Method for detecting contaminant cells in a stem cell derived cell population |
Family Cites Families (55)
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|---|---|---|---|---|
| US5523226A (en) * | 1993-05-14 | 1996-06-04 | Biotechnology Research And Development Corp. | Transgenic swine compositions and methods |
| ATE274510T1 (de) | 1998-06-19 | 2004-09-15 | Chiron Corp | Glycogen synthase kinase 3 inhibitoren |
| JP2004531262A (ja) | 2001-05-15 | 2004-10-14 | ラッパポート ファミリー インスチチュート フォア リサーチ イン ザ メディカル サイエンシズ | ヒト胚性幹細胞由来インスリン産生細胞 |
| CN1602351B (zh) | 2001-12-07 | 2011-03-30 | 杰龙公司 | 源自人胚胎干细胞的胰岛细胞 |
| CN1671835A (zh) | 2001-12-28 | 2005-09-21 | 塞拉提斯股份公司 | 一种建立多能人胚泡衍生的干细胞的方法 |
| SG149061A1 (en) * | 2003-12-23 | 2009-01-29 | Cythera Inc | Definitive endoderm |
| US7625753B2 (en) | 2003-12-23 | 2009-12-01 | Cythera, Inc. | Expansion of definitive endoderm cells |
| DK1740612T3 (da) | 2004-04-27 | 2019-10-07 | Viacyte Inc | Pdx1 udtrykkende endoderm |
| WO2007042225A2 (en) | 2005-10-07 | 2007-04-19 | Cellartis Ab | A method for obtaining a xeno-free hbs cell line |
| EP4112718A1 (en) | 2006-03-02 | 2023-01-04 | ViaCyte, Inc. | Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production |
| GB0615327D0 (en) | 2006-03-30 | 2006-09-13 | Univ Edinburgh | Culture medium containing kinase inhibitors and uses thereof |
| EP2021462B1 (en) | 2006-04-28 | 2019-01-09 | Lifescan, Inc. | Differentiation of human embryonic stem cells |
| US8741643B2 (en) * | 2006-04-28 | 2014-06-03 | Lifescan, Inc. | Differentiation of pluripotent stem cells to definitive endoderm lineage |
| CA2654196A1 (en) | 2006-06-02 | 2007-12-13 | University Of Georgia Research Foundation, Inc. | Pancreatic and liver endoderm cells and tissue by differentiation of definitive endoderm cells obtained from human embryonic stems |
| CN101641436A (zh) * | 2007-01-30 | 2010-02-03 | 佐治亚大学研究基金会 | 用于产生内胚层和中胚层细胞系及多能游走细胞(mmc)的早期中胚层细胞即稳定的中内胚层细胞群 |
| JP5646990B2 (ja) | 2007-04-23 | 2014-12-24 | ストワーズ インスティテュート フォー メディカル リサーチ | 幹細胞自己複製のための方法及び組成物 |
| US9080145B2 (en) * | 2007-07-01 | 2015-07-14 | Lifescan Corporation | Single pluripotent stem cell culture |
| EP3957716A1 (en) | 2007-07-18 | 2022-02-23 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
| SG183067A1 (en) | 2007-07-20 | 2012-08-30 | Cellartis Ab | A novel population of hepatocytes derived via definitive endoderm (de-hep) from human blastocysts derived stem cells |
| PL2185691T3 (pl) | 2007-07-31 | 2018-08-31 | Lifescan, Inc. | Różnicowanie pluripotencjalnych komórek macierzystych poprzez zastosowanie ludzkich komórek odżywczych |
| CA2695225C (en) | 2007-07-31 | 2021-06-01 | Lifescan, Inc. | Differentiation of human embryonic stem cells to pancreatic endocrine |
| EP2195417A1 (en) * | 2007-08-24 | 2010-06-16 | University Court of the University of Edinburgh | Regionalised endoderm cells and uses thereof |
| US8623648B2 (en) | 2008-04-24 | 2014-01-07 | Janssen Biotech, Inc. | Treatment of pluripotent cells |
| US7939322B2 (en) | 2008-04-24 | 2011-05-10 | Centocor Ortho Biotech Inc. | Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm |
| WO2009137844A2 (en) | 2008-05-09 | 2009-11-12 | Vistagen Therapeutics, Inc. | Pancreatic endocrine progenitor cells derived from pluripotent stem cells |
| US20090298178A1 (en) * | 2008-06-03 | 2009-12-03 | D Amour Kevin Allen | Growth factors for production of definitive endoderm |
| WO2009154606A1 (en) * | 2008-06-03 | 2009-12-23 | Cythera, Inc. | Growth factors for production of definitive endoderm |
| RU2011103183A (ru) | 2008-06-30 | 2012-08-10 | Сентокор Орто Байотек Инк. (Us) | Дифференцирование плюрипотентных стволовых клеток |
| EP2942392B1 (en) | 2008-06-30 | 2018-10-03 | Janssen Biotech, Inc. | Differentiation of pluripotent stem cells |
| US20100028307A1 (en) | 2008-07-31 | 2010-02-04 | O'neil John J | Pluripotent stem cell differentiation |
| US8278105B2 (en) * | 2008-09-09 | 2012-10-02 | University Of Southern California | Induction, propagation and isolation of liver progenitor cells |
| CN101684454B (zh) * | 2008-09-25 | 2012-08-15 | 中国科学院上海生命科学研究院 | 一种定型内胚层细胞的制备和分离方法 |
| ES2727950T3 (es) | 2008-10-31 | 2019-10-21 | Janssen Biotech Inc | Diferenciación de células madre embrionarias humanas en linaje endocrino pancreático |
| MX2011004565A (es) | 2008-10-31 | 2011-07-28 | Centocor Ortho Biotech Inc | Diferenciacion de celulas madre embrionarias humanas al linaje endocrino pancreatico. |
| AU2009313870B2 (en) | 2008-11-14 | 2013-07-11 | Viacyte, Inc. | Encapsulation of pancreatic cells derived from human pluripotent stem cells |
| WO2010091241A2 (en) | 2009-02-06 | 2010-08-12 | President And Fellows Of Harvard College | Compositions and methods for promoting the generation of definitive endoderm |
| EP2421957B1 (en) | 2009-04-22 | 2020-11-18 | Viacyte, Inc. | Cell compositions derived from dedifferentiated reprogrammed cells |
| EP2435558A4 (en) | 2009-05-27 | 2013-05-22 | Salk Inst For Biological Studi | GENETICALLY CORRECTED NON-DISABLED INDUCED PLURIPOTENTER STEM CELLS |
| CN102482640B (zh) * | 2009-07-20 | 2015-03-11 | 詹森生物科技公司 | 人胚胎干细胞的分化 |
| BR112012001480A2 (pt) | 2009-07-20 | 2015-09-01 | Janssen Biotech Inc | Diferenciação de células-tronco embriônicas humanas |
| MX343786B (es) | 2009-12-23 | 2016-11-22 | Janssen Biotech Inc | Diferenciacion de celulas madre embrionarias humanas. |
| ES2633648T3 (es) | 2009-12-23 | 2017-09-22 | Janssen Biotech, Inc. | Diferenciación de células madre embrionarias humanas |
| EP2505639B1 (en) | 2009-12-29 | 2018-09-19 | Takeda Pharmaceutical Company Limited | Method for manufacturing pancreatic-hormone-producing cells |
| SG10201501503VA (en) | 2010-03-01 | 2015-04-29 | Janssen Biotech Inc | Methods for purifying cells derived from pluripotent stem cells |
| WO2011140441A2 (en) | 2010-05-06 | 2011-11-10 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into intestinal tissues through directed differentiation |
| EP3498825A1 (en) | 2010-05-12 | 2019-06-19 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
| US9085757B2 (en) | 2010-06-17 | 2015-07-21 | Regents Of The University Of Minnesota | Production of insulin producing cells |
| JP2013533319A (ja) | 2010-08-12 | 2013-08-22 | ヤンセン バイオテツク,インコーポレーテツド | 膵内分泌腺前駆体細胞による糖尿病の治療 |
| CA2809300A1 (en) | 2010-08-31 | 2012-03-08 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
| EP3211070A1 (en) | 2010-08-31 | 2017-08-30 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
| US9062292B2 (en) | 2010-09-13 | 2015-06-23 | Enzo Life Sciences Inc. | Mutant T7 polymerases |
| WO2012070014A2 (en) | 2010-11-26 | 2012-05-31 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Identification of novel cell surface markers for pancreatic progenitor cells and definite endodermal cells |
| CA2820419A1 (en) | 2010-12-08 | 2012-06-14 | Viacyte, Inc. | Agents and methods for inhibiting human pluripotent stem cell growth |
| WO2012081029A1 (en) | 2010-12-15 | 2012-06-21 | Kadimastem Ltd. | Insulin producing cells derived from pluripotent stem cells |
| US20140234963A1 (en) | 2011-06-21 | 2014-08-21 | Novo Nordisk A/S | Efficient induction of definitive endoderm from pluripotent stem cells |
-
2012
- 2012-06-21 US US14/127,296 patent/US20140234963A1/en not_active Abandoned
- 2012-06-21 ES ES12735227T patent/ES2902650T3/es active Active
- 2012-06-21 JP JP2014516354A patent/JP6312591B2/ja active Active
- 2012-06-21 CN CN201280040881.9A patent/CN103890167A/zh active Pending
- 2012-06-21 WO PCT/EP2012/062013 patent/WO2012175633A1/en not_active Ceased
- 2012-06-21 CN CN201810066827.0A patent/CN108220224A/zh active Pending
- 2012-06-21 EP EP12735227.6A patent/EP2723852B1/en active Active
-
2017
- 2017-09-15 US US15/705,705 patent/US10487313B2/en active Active
-
2019
- 2019-11-25 US US16/694,040 patent/US20200190477A1/en not_active Abandoned
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