JP2014503207A5 - - Google Patents
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- JP2014503207A5 JP2014503207A5 JP2013544833A JP2013544833A JP2014503207A5 JP 2014503207 A5 JP2014503207 A5 JP 2014503207A5 JP 2013544833 A JP2013544833 A JP 2013544833A JP 2013544833 A JP2013544833 A JP 2013544833A JP 2014503207 A5 JP2014503207 A5 JP 2014503207A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- template
- strand
- reverse
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 150000007523 nucleic acids Chemical class 0.000 claims 21
- 238000000034 method Methods 0.000 claims 20
- 108020004707 nucleic acids Proteins 0.000 claims 16
- 102000039446 nucleic acids Human genes 0.000 claims 16
- 230000000295 complement effect Effects 0.000 claims 13
- 108091093088 Amplicon Proteins 0.000 claims 8
- 230000003321 amplification Effects 0.000 claims 6
- 239000007791 liquid phase Substances 0.000 claims 6
- 238000003199 nucleic acid amplification method Methods 0.000 claims 6
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 4
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims 4
- 239000011324 bead Substances 0.000 claims 3
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims 2
- 229930024421 Adenine Natural products 0.000 claims 2
- 102000053642 Catalytic RNA Human genes 0.000 claims 2
- 108090000994 Catalytic RNA Proteins 0.000 claims 2
- 229960000643 adenine Drugs 0.000 claims 2
- 238000004873 anchoring Methods 0.000 claims 2
- 238000009396 hybridization Methods 0.000 claims 2
- 238000002844 melting Methods 0.000 claims 2
- 230000008018 melting Effects 0.000 claims 2
- 108091008146 restriction endonucleases Proteins 0.000 claims 2
- 108091092562 ribozyme Proteins 0.000 claims 2
- 229940113082 thymine Drugs 0.000 claims 2
- 229940035893 uracil Drugs 0.000 claims 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 238000003491 array Methods 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 230000001419 dependent effect Effects 0.000 claims 1
- 238000010348 incorporation Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 230000004807 localization Effects 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 239000012071 phase Substances 0.000 claims 1
- 230000002285 radioactive effect Effects 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201061424599P | 2010-12-17 | 2010-12-17 | |
| US61/424,599 | 2010-12-17 | ||
| US201161445324P | 2011-02-22 | 2011-02-22 | |
| US61/445,324 | 2011-02-22 | ||
| US201161451919P | 2011-03-11 | 2011-03-11 | |
| US61/451,919 | 2011-03-11 | ||
| US201161526478P | 2011-08-23 | 2011-08-23 | |
| US61/526,478 | 2011-08-23 | ||
| US201161552660P | 2011-10-28 | 2011-10-28 | |
| US61/552,660 | 2011-10-28 | ||
| PCT/US2011/065535 WO2012083189A2 (en) | 2010-12-17 | 2011-12-16 | Methods, compositions, systems, apparatuses and kits for nucleic acid amplification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014503207A JP2014503207A (ja) | 2014-02-13 |
| JP2014503207A5 true JP2014503207A5 (OSRAM) | 2014-11-06 |
| JP5907990B2 JP5907990B2 (ja) | 2016-05-11 |
Family
ID=45478545
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013544833A Active JP5907990B2 (ja) | 2010-12-17 | 2011-12-16 | 核酸増幅のための方法、組成物、システム、装置およびキット |
Country Status (5)
| Country | Link |
|---|---|
| US (10) | US20120156728A1 (OSRAM) |
| EP (3) | EP2652148B1 (OSRAM) |
| JP (1) | JP5907990B2 (OSRAM) |
| CN (3) | CN103370425B (OSRAM) |
| WO (1) | WO2012083189A2 (OSRAM) |
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