JP2013543370A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2013543370A5 JP2013543370A5 JP2013521143A JP2013521143A JP2013543370A5 JP 2013543370 A5 JP2013543370 A5 JP 2013543370A5 JP 2013521143 A JP2013521143 A JP 2013521143A JP 2013521143 A JP2013521143 A JP 2013521143A JP 2013543370 A5 JP2013543370 A5 JP 2013543370A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- target nucleic
- solid support
- liquid samples
- support material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108020004707 nucleic acids Proteins 0.000 claims 22
- 150000007523 nucleic acids Chemical class 0.000 claims 22
- 102000039446 nucleic acids Human genes 0.000 claims 22
- 238000000034 method Methods 0.000 claims 19
- 239000007788 liquid Substances 0.000 claims 12
- 239000000463 material Substances 0.000 claims 8
- 239000007787 solid Substances 0.000 claims 8
- 230000003321 amplification Effects 0.000 claims 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims 5
- 239000012139 lysis buffer Substances 0.000 claims 4
- 239000003153 chemical reaction reagent Substances 0.000 claims 3
- 102100034343 Integrase Human genes 0.000 claims 2
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 238000000926 separation method Methods 0.000 claims 2
- 238000013518 transcription Methods 0.000 claims 2
- 230000035897 transcription Effects 0.000 claims 2
- 230000003612 virological effect Effects 0.000 claims 2
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims 1
- 230000002378 acidificating effect Effects 0.000 claims 1
- 150000001298 alcohols Chemical class 0.000 claims 1
- 239000000872 buffer Substances 0.000 claims 1
- 210000000234 capsid Anatomy 0.000 claims 1
- 230000001413 cellular effect Effects 0.000 claims 1
- 230000003196 chaotropic effect Effects 0.000 claims 1
- 239000003638 chemical reducing agent Substances 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 239000012149 elution buffer Substances 0.000 claims 1
- 230000002934 lysing effect Effects 0.000 claims 1
- 239000002245 particle Substances 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 239000011534 wash buffer Substances 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36897010P | 2010-07-29 | 2010-07-29 | |
| US61/368,970 | 2010-07-29 | ||
| EP10175533 | 2010-09-07 | ||
| EP10175533.8 | 2010-09-07 | ||
| PCT/EP2011/062958 WO2012013733A1 (en) | 2010-07-29 | 2011-07-27 | Generic sample preparation |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017086559A Division JP6348202B2 (ja) | 2010-07-29 | 2017-04-25 | 一般的な試料調製 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013543370A JP2013543370A (ja) | 2013-12-05 |
| JP2013543370A5 true JP2013543370A5 (enExample) | 2014-05-22 |
| JP6169489B2 JP6169489B2 (ja) | 2017-07-26 |
Family
ID=43048869
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013521143A Active JP6169489B2 (ja) | 2010-07-29 | 2011-07-27 | 一般的な試料調製 |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP2598654B2 (enExample) |
| JP (1) | JP6169489B2 (enExample) |
| CN (2) | CN107058617A (enExample) |
| CA (1) | CA2802563C (enExample) |
| DE (1) | DE112011102524T5 (enExample) |
| ES (1) | ES2617258T5 (enExample) |
| WO (1) | WO2012013733A1 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8609340B2 (en) | 2010-07-29 | 2013-12-17 | Roche Molecular Systems, Inc. | Control nucleic acids for multiple parameters |
| US9725754B2 (en) | 2010-07-29 | 2017-08-08 | Sean F. Boyle | Generic sample preparation |
| WO2013113748A1 (en) * | 2012-02-02 | 2013-08-08 | Primer Design Ltd | Method for detecting and genotyping target nucleic acid |
| ES2567091T3 (es) | 2012-04-05 | 2016-04-19 | F. Hoffmann-La Roche Ag | Compuestos de amina para la preparación selectiva de muestras biológicas |
| KR20150054844A (ko) * | 2012-09-19 | 2015-05-20 | 베크만 컬터, 인코포레이티드 | 핵산 추출에서의 2가 이온, 프로테아제, 계면활성제, 및 낮은 pH의 사용 |
| DE102014111210B3 (de) * | 2014-08-06 | 2016-01-07 | GFE Blut mbH | Verfahren und Vorrichtung zum automatisierten Bearbeiten von gepoolten Proben |
| US20170283888A1 (en) * | 2015-12-21 | 2017-10-05 | Roche Molecular Systems, Inc. | Use of rnase h for the selective amplification of viral dna |
| EP3299471B1 (en) | 2016-09-23 | 2019-10-23 | Roche Diagniostics GmbH | Methods for determining the amount of a nucleic acid of interest in an unprocessed sample |
| CN107418951B (zh) * | 2017-08-11 | 2021-01-15 | 浙江省林业科学研究院 | 一种低污染及高敏感的低成本通用菌种分子鉴定方法 |
| GB201812428D0 (en) * | 2018-07-31 | 2018-09-12 | Bg Res Ltd | Improved method for pathogen detection |
| JP2024518331A (ja) * | 2021-04-29 | 2024-05-01 | アボット・ラボラトリーズ | 生体試料のハイスループット核酸検査 |
Family Cites Families (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE373444C (de) | 1923-04-12 | Otto Ulrich | Handstaubsauger | |
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US5455170A (en) | 1986-08-22 | 1995-10-03 | Hoffmann-La Roche Inc. | Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 |
| DE3734442A1 (de) | 1987-10-12 | 1989-04-27 | Kernforschungsanlage Juelich | Verfahren und vorrichtung zur bestrahlung grosser flaechen mit ionen |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| ATE138106T1 (de) | 1988-07-20 | 1996-06-15 | David Segev | Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen |
| NL8900725A (nl) | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| US5035996A (en) | 1989-06-01 | 1991-07-30 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
| KR950013953B1 (ko) | 1990-01-26 | 1995-11-18 | 애보트 래보라토리즈 | 리가제 연쇄 반응에 적용가능한 표적 핵산의 증폭 방법 |
| US5474895A (en) | 1990-11-14 | 1995-12-12 | Siska Diagnostics Inc. | Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor |
| GB9100212D0 (en) | 1991-01-05 | 1991-02-20 | Gormley Alan | Dog excrement collector |
| US5386024A (en) | 1993-02-10 | 1995-01-31 | Gen-Probe Incorporated | Method to prepare nucleic acids from a biological sample using low pH and acid protease |
| DE19520398B4 (de) * | 1995-06-08 | 2009-04-16 | Roche Diagnostics Gmbh | Magnetisches Pigment |
| EP1442794B1 (en) | 1996-06-04 | 2006-03-29 | University Of Utah Research Foundation | System and method for carrying out and monitoring biological processes |
| DE69739357D1 (de) | 1996-06-04 | 2009-05-28 | Univ Utah Res Found | Überwachung der hybridisierung während pcr |
| US5939262A (en) | 1996-07-03 | 1999-08-17 | Ambion, Inc. | Ribonuclease resistant RNA preparation and utilization |
| US5981235A (en) | 1996-07-29 | 1999-11-09 | Promega Corporation | Methods for isolating nucleic acids using alkaline protease |
| DE19743518A1 (de) * | 1997-10-01 | 1999-04-15 | Roche Diagnostics Gmbh | Automatisierbare universell anwendbare Probenvorbereitungsmethode |
| JP3812696B2 (ja) | 1997-11-17 | 2006-08-23 | 東洋紡績株式会社 | リボ核酸の抽出方法 |
| ES2308865T3 (es) * | 1999-03-11 | 2008-12-01 | Whatman, Inc. | Medio solido y procedimiento para el almacenamiento y la rapida purificacion de acido nucleico. |
| ATE411110T1 (de) | 1999-07-19 | 2008-10-15 | Biomerieux Bv | Verfahren zum mengen von magnetteilchen mit einer flüssigkeit |
| EP1204741B1 (en) | 1999-08-20 | 2004-10-20 | Promega Corporation | Simultaneous isolation and quantitation of dna |
| CA2392009C (en) * | 1999-11-17 | 2009-07-14 | Roche Diagnostics Gmbh | Magnetic glass particles, method for their preparation and uses thereof |
| US7238795B2 (en) | 2000-08-03 | 2007-07-03 | Roche Molecular Systems, Inc. | Nucleic acid binding compounds containing pyrazolo[3,4-d]pyrimidine analogues of purin-2,6-diamine and their uses |
| EP1201753B1 (en) | 2000-10-31 | 2008-04-23 | Boehringer Mannheim Gmbh | Methods for the analysis of non-proteinaceous components using a protease from a bacillus strain |
| EP1466018B2 (en) | 2002-01-08 | 2015-08-12 | Roche Diagnostics GmbH | Use of silica material in an amplification reaction |
| US20040180445A1 (en) * | 2003-03-12 | 2004-09-16 | Domanico Michael J. | Methods and compositions for purification of nucleic acid from a host cell |
| AU2005200670B2 (en) | 2004-02-20 | 2007-05-03 | F. Hoffmann-La Roche Ag | Adsorption of nucleic acids to a solid phase |
| EP1690938A1 (de) | 2005-02-11 | 2006-08-16 | Qiagen GmbH | Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden |
| US8163535B2 (en) * | 2006-06-26 | 2012-04-24 | Blood Cell Storage, Inc. | Devices and processes for nucleic acid extraction |
| JP2009544955A (ja) | 2006-07-28 | 2009-12-17 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 試料を処理するための装置 |
| US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| JP5638806B2 (ja) | 2006-11-03 | 2014-12-10 | シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレーテッドＳｉｅｍｅｎｓ Ｈｅａｌｔｈｃａｒｅ Ｄｉａｇｎｏｓｔｉｃｓ Ｉｎｃ． | ポリメラーゼ連鎖反応試験のためのランダムアクセスシステムおよび方法 |
| EP1932913B1 (en) | 2006-12-11 | 2013-01-16 | Roche Diagnostics GmbH | Nucleic acid isolation using polidocanol and derivatives |
| EP2126074B1 (en) | 2006-12-13 | 2010-12-01 | Roche Diagnostics GmbH | Use of acetals for the isolation of nucleic acids |
| WO2008071384A1 (en) | 2006-12-13 | 2008-06-19 | Roche Diagnostics Gmbh | Use of tde for the isolation of nucleic acids |
| EP2130929B1 (en) | 2008-06-06 | 2013-10-09 | F. Hoffmann-La Roche AG | Internally controlled multiplex detection and quantification of microbial nucleic acids |
-
2011
- 2011-07-27 CN CN201710127528.9A patent/CN107058617A/zh active Pending
- 2011-07-27 CN CN2011800358181A patent/CN103025891A/zh active Pending
- 2011-07-27 DE DE112011102524T patent/DE112011102524T5/de active Pending
- 2011-07-27 ES ES11735891T patent/ES2617258T5/es active Active
- 2011-07-27 CA CA2802563A patent/CA2802563C/en active Active
- 2011-07-27 EP EP11735891.1A patent/EP2598654B2/en active Active
- 2011-07-27 JP JP2013521143A patent/JP6169489B2/ja active Active
- 2011-07-27 WO PCT/EP2011/062958 patent/WO2012013733A1/en not_active Ceased
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Thatcher | DNA/RNA preparation for molecular detection | |
| EP3470532B1 (en) | Method for collection and amplification of circulating nucleic acids | |
| US20100159460A1 (en) | Isolation of nucleic acids on surfaces | |
| Zhang et al. | A microfluidic liquid phase nucleic acid purification chip to selectively isolate DNA or RNA from low copy/single bacterial cells in minute sample volume followed by direct on-chip quantitative PCR assay | |
| US10876106B2 (en) | Gene analysis system | |
| ES2827307T3 (es) | Métodos y composiciones para la síntesis de ADNc y la elaboración del perfil del transcriptoma de células individuales utilizando una reacción de cambio de plantilla | |
| US20110224419A1 (en) | Method For Isolating And Purifying Nucleic Acids | |
| Glais et al. | Detection and characterization of viral species/subspecies using isothermal recombinase polymerase amplification (RPA) assays | |
| EP2917344B1 (en) | Methods for one step nucleic acid amplification of non-eluted samples | |
| US20170029810A1 (en) | Nucleic acid purification method | |
| US9260711B2 (en) | Solid matrix for one step nucleic acid amplification | |
| CN111801428B (zh) | 一种获得单细胞mRNA序列的方法 | |
| JP2013543371A5 (enExample) | ||
| DK201000356A (en) | New nucleotide sequence amplification Method | |
| JP2008520212A5 (enExample) | ||
| EP3239710A1 (en) | Method for separating biotinylated nucleic acid | |
| JP2014507155A (ja) | マイクロ流体素子を基礎とした核酸精製方法 | |
| Wen et al. | A microfluidic dual-aptamer sandwich assay for rapid and cost-effective detection of recombinant proteins | |
| CN117867167A (zh) | 一种基于RPA-CRISPR/Cas12a检测新布尼亚病毒的引物组及其应用 | |
| JP2013542713A5 (enExample) | ||
| WO2019116800A1 (ja) | 単一細胞の網羅的３'末端遺伝子発現解析法 | |
| Seo et al. | Identification of long noncoding RNA-protein interactions through in vitro RNA pull-down assay with plant nuclear extracts | |
| Das et al. | A quick and cost-effective method for DNA-free total RNA isolation using magnetic silica beads | |
| US20230074066A1 (en) | Compositions and methods for rapid rna-adenylation and rna sequencing | |
| WO2014041093A1 (en) | Solid matrix for one step nucleic acid amplification |