JP2013542713A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2013542713A5 JP2013542713A5 JP2013521142A JP2013521142A JP2013542713A5 JP 2013542713 A5 JP2013542713 A5 JP 2013542713A5 JP 2013521142 A JP2013521142 A JP 2013521142A JP 2013521142 A JP2013521142 A JP 2013521142A JP 2013542713 A5 JP2013542713 A5 JP 2013542713A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- target nucleic
- reaction vessel
- polymerase
- dna polymerase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108020004707 nucleic acids Proteins 0.000 claims 40
- 150000007523 nucleic acids Chemical class 0.000 claims 40
- 102000039446 nucleic acids Human genes 0.000 claims 40
- 238000000034 method Methods 0.000 claims 18
- 230000003321 amplification Effects 0.000 claims 12
- 238000003199 nucleic acid amplification method Methods 0.000 claims 12
- 102100034343 Integrase Human genes 0.000 claims 10
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 10
- 230000000694 effects Effects 0.000 claims 10
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 8
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 8
- 239000007788 liquid Substances 0.000 claims 8
- 239000003153 chemical reaction reagent Substances 0.000 claims 7
- 239000000463 material Substances 0.000 claims 7
- 239000007787 solid Substances 0.000 claims 6
- 241000589596 Thermus Species 0.000 claims 4
- 238000000926 separation method Methods 0.000 claims 4
- 238000013518 transcription Methods 0.000 claims 4
- 230000035897 transcription Effects 0.000 claims 4
- 230000035772 mutation Effects 0.000 claims 3
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims 1
- 108010017826 DNA Polymerase I Proteins 0.000 claims 1
- 102000004594 DNA Polymerase I Human genes 0.000 claims 1
- 108010076551 DNA polymerase C Proteins 0.000 claims 1
- 101000902592 Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) DNA polymerase Proteins 0.000 claims 1
- 241000206213 Thermosipho africanus Species 0.000 claims 1
- 241000204666 Thermotoga maritima Species 0.000 claims 1
- 241000204664 Thermotoga neapolitana Species 0.000 claims 1
- 241000589501 Thermus caldophilus Species 0.000 claims 1
- 241000589498 Thermus filiformis Species 0.000 claims 1
- 108010085671 Thermus thermophilus DNA polymerase Proteins 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 230000003612 virological effect Effects 0.000 claims 1
- 239000011534 wash buffer Substances 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36896610P | 2010-07-29 | 2010-07-29 | |
| US61/368,966 | 2010-07-29 | ||
| EP10175545 | 2010-09-07 | ||
| EP10175545.2 | 2010-09-07 | ||
| PCT/EP2011/062957 WO2012013732A1 (en) | 2010-07-29 | 2011-07-27 | Generic pcr |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013542713A JP2013542713A (ja) | 2013-11-28 |
| JP2013542713A5 true JP2013542713A5 (enExample) | 2014-05-22 |
| JP5972265B2 JP5972265B2 (ja) | 2016-08-17 |
Family
ID=43033435
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013521142A Active JP5972265B2 (ja) | 2010-07-29 | 2011-07-27 | ジェネリックpcr |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP2598653B1 (enExample) |
| JP (1) | JP5972265B2 (enExample) |
| CN (1) | CN103270171B (enExample) |
| CA (1) | CA2802550C (enExample) |
| ES (1) | ES2644797T3 (enExample) |
| WO (1) | WO2012013732A1 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8609340B2 (en) | 2010-07-29 | 2013-12-17 | Roche Molecular Systems, Inc. | Control nucleic acids for multiple parameters |
| ES2644949T3 (es) * | 2014-08-28 | 2017-12-01 | F. Hoffmann-La Roche Ag | Oligonucleótidos para controlar la amplificación de ácidos nucleicos |
| CN120060564A (zh) * | 2016-09-07 | 2025-05-30 | 圣文森特医院悉尼有限公司 | 检测慢病毒的方法 |
| CN108642145A (zh) * | 2018-05-18 | 2018-10-12 | 上海默礼生物医药科技有限公司 | 一种用于核酸实时恒温扩增的试剂、扩增体系及其扩增方法和应用 |
| GB201812192D0 (en) | 2018-07-26 | 2018-09-12 | Ttp Plc | Variable temperature reactor, heater and control circuit for the same |
| CA3126398A1 (en) * | 2019-01-17 | 2020-07-23 | Northwestern University | Rapid reverse transcription quantitative polymerase chain reaction |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE373444C (de) | 1923-04-12 | Otto Ulrich | Handstaubsauger | |
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US5455170A (en) | 1986-08-22 | 1995-10-03 | Hoffmann-La Roche Inc. | Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| JP2955759B2 (ja) | 1988-07-20 | 1999-10-04 | セゲブ・ダイアグノスティックス・インコーポレイテッド | 核酸配列を増幅及び検出する方法 |
| US5035996A (en) | 1989-06-01 | 1991-07-30 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
| ES2089038T3 (es) | 1990-01-26 | 1996-10-01 | Abbott Lab | Procedimiento mejorado para amplificar acidos nucleicos blanco aplicable para la reaccion en cadena de polimerasa y ligasa. |
| WO1992008808A1 (en) | 1990-11-14 | 1992-05-29 | Siska Diagnostics, Inc. | Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor |
| US5386024A (en) | 1993-02-10 | 1995-01-31 | Gen-Probe Incorporated | Method to prepare nucleic acids from a biological sample using low pH and acid protease |
| US5968729A (en) * | 1994-06-10 | 1999-10-19 | Kosak; Kenneth M. | Use of centrifugation to prepare a retractable seal over reagents in a reaction container |
| AU726501B2 (en) | 1996-06-04 | 2000-11-09 | University Of Utah Research Foundation | Monitoring hybridization during PCR |
| ATE390491T1 (de) | 1996-06-04 | 2008-04-15 | Univ Utah Res Found | Behälter zum durchführen und beobachten biologischer prozesse |
| US5981235A (en) | 1996-07-29 | 1999-11-09 | Promega Corporation | Methods for isolating nucleic acids using alkaline protease |
| US6261770B1 (en) * | 1997-05-13 | 2001-07-17 | Display Systems Biotech Aps | Method to clone mRNAs |
| MXPA01007352A (es) * | 1999-01-19 | 2003-06-06 | Somalogic Inc | Metodo y aparato para la generacion automatizada de ligandos de acido nucleico. |
| DK1232502T3 (da) | 1999-11-17 | 2006-05-22 | Roche Diagnostics Gmbh | Magnetiske glaspartikler, fremgangsmåde til deres fremstilling samt anvendelser deraf |
| JP4331476B2 (ja) | 2000-08-03 | 2009-09-16 | エフ.ホフマン−ラ ロシュ アーゲー | プリン−2,6−ジアミンのピラゾロ[3,4−d]ピリミジンアナローグを含有する核酸結合性化合物およびそれらの使用 |
| ES2305023T3 (es) | 2000-10-31 | 2008-11-01 | F. Hoffmann-La Roche Ag | Metodos para el analisis de componentes no proteinaceos usando una proteasa de una cepa de bacilo. |
| US8962293B2 (en) * | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| US8895240B2 (en) * | 2008-05-19 | 2014-11-25 | E I Du Pont De Nemours And Company | Method, kit and system for collecting and processing samples for DNA and RNA detection |
| EP2130929B1 (en) * | 2008-06-06 | 2013-10-09 | F. Hoffmann-La Roche AG | Internally controlled multiplex detection and quantification of microbial nucleic acids |
-
2011
- 2011-07-27 CA CA2802550A patent/CA2802550C/en active Active
- 2011-07-27 CN CN201180046829.XA patent/CN103270171B/zh active Active
- 2011-07-27 ES ES11735890.3T patent/ES2644797T3/es active Active
- 2011-07-27 JP JP2013521142A patent/JP5972265B2/ja active Active
- 2011-07-27 WO PCT/EP2011/062957 patent/WO2012013732A1/en not_active Ceased
- 2011-07-27 EP EP11735890.3A patent/EP2598653B1/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR102313174B1 (ko) | 순환 핵산의 수집 및 증폭을 위한 방법 및 장치 | |
| John et al. | Genome‐scale mapping of DNase I hypersensitivity | |
| JP6963505B2 (ja) | 鎖置換ポリメラーゼを用いた固相核酸標的捕捉及び複製 | |
| JP2013542713A5 (enExample) | ||
| AU2018383191A1 (en) | A non-invasive method for monitoring transplanted organ status in organ-transplant recipients | |
| US10100358B2 (en) | Methods and compositions for reducing redundant molecular barcodes created in primer extension reactions | |
| JP2013543370A5 (enExample) | ||
| JP2013543371A5 (enExample) | ||
| US9206468B2 (en) | One-step method of elution of DNA from blood samples | |
| JP2012055311A (ja) | 増幅用一般バッファー | |
| EP3105324B1 (en) | Ngs systems control and methods involving the same | |
| CA3021914C (en) | Blood cell lysis reagent | |
| Alves et al. | A novel real‐time PCR method for KIR genotyping | |
| CN105671142A (zh) | 一种检测人类pear1基因多态性的试剂盒及其应用 | |
| US10941453B1 (en) | High throughput detection of pathogen RNA in clinical specimens | |
| Ullmann et al. | Comparative clinical sample preparation of DNA and RNA viral nucleic acids for a commercial deep sequencing system (Illumina MiSeq®) | |
| US20200216903A1 (en) | Mitochondrial Disease Genetic Diagnostics | |
| Sun | Nucleic extraction and amplification | |
| Ahn et al. | Array comparative genomic hybridization (array CGH) for detection of genomic copy number variants | |
| Wan et al. | High-throughput plasmid cDNA library screening | |
| CN107190101A (zh) | 一种用于胚胎植入前染色体异常检测的方法及试剂盒 | |
| US20150191800A1 (en) | Detection of cytomegalovirus dna using amplification from blood samples | |
| WO2020159608A1 (en) | Cost-effective detection of low frequency genetic variation | |
| CN104789673B (zh) | rs1800818在检测新布尼亚病毒引起的发热伴血小板减少综合征中的应用 | |
| Smith et al. | An in-solution hybridisation method for the isolation of pathogen DNA from human DNA-rich clinical samples for analysis by NGS |