JP5972265B2 - ジェネリックｐｃｒ - Google Patents

ジェネリックｐｃｒ Download PDF

Info

Publication number
JP5972265B2
JP5972265B2 JP2013521142A JP2013521142A JP5972265B2 JP 5972265 B2 JP5972265 B2 JP 5972265B2 JP 2013521142 A JP2013521142 A JP 2013521142A JP 2013521142 A JP2013521142 A JP 2013521142A JP 5972265 B2 JP5972265 B2 JP 5972265B2
Authority
JP
Japan
Prior art keywords
nucleic acid
target nucleic
amplification
polymerase
reaction vessel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2013521142A
Other languages
English (en)
Japanese (ja)
Other versions
JP2013542713A5 (enExample
JP2013542713A (ja
Inventor
アイクホフ，マイケ
ヒツィガー，ニクラス
ツィンマーマン，ディルク
マイヤーズ，トーマス，ダブリュ．
ウィル，ステフェン，ゴードン
スミス，エドワード，エス．
ウィルツ，ハイケ
ムスマン，アンケ
ニューハウス，クリストファー
ニュートン，ニコラス
ニーミーク，ジョン，ティー．
シェーンブルンナー，ナンシー
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of JP2013542713A publication Critical patent/JP2013542713A/ja
Publication of JP2013542713A5 publication Critical patent/JP2013542713A5/ja
Application granted granted Critical
Publication of JP5972265B2 publication Critical patent/JP5972265B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2013521142A 2010-07-29 2011-07-27 ジェネリックｐｃｒ Active JP5972265B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US36896610P 2010-07-29 2010-07-29
US61/368,966 2010-07-29
EP10175545.2 2010-09-07
EP10175545 2010-09-07
PCT/EP2011/062957 WO2012013732A1 (en) 2010-07-29 2011-07-27 Generic pcr

Publications (3)

Publication Number Publication Date
JP2013542713A JP2013542713A (ja) 2013-11-28
JP2013542713A5 JP2013542713A5 (enExample) 2014-05-22
JP5972265B2 true JP5972265B2 (ja) 2016-08-17

Family

ID=43033435

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2013521142A Active JP5972265B2 (ja) 2010-07-29 2011-07-27 ジェネリックｐｃｒ

Country Status (6)

Country Link
EP (1) EP2598653B1 (enExample)
JP (1) JP5972265B2 (enExample)
CN (1) CN103270171B (enExample)
CA (1) CA2802550C (enExample)
ES (1) ES2644797T3 (enExample)
WO (1) WO2012013732A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8609340B2 (en) 2010-07-29 2013-12-17 Roche Molecular Systems, Inc. Control nucleic acids for multiple parameters
EP2990491B1 (en) * 2014-08-28 2017-07-12 F. Hoffmann-La Roche AG Oligonucleotides for controlling amplification of nucleic acids
EP3510172B1 (en) * 2016-09-07 2023-11-29 St Vincent's Hospital Sydney Limited Methods of detecting hiv
CN108642145A (zh) * 2018-05-18 2018-10-12 上海默礼生物医药科技有限公司 一种用于核酸实时恒温扩增的试剂、扩增体系及其扩增方法和应用
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
MX2021008567A (es) * 2019-01-17 2021-09-21 Univ Northwestern Reaccion en cadena de la polimerasa cuantitativa con retrotranscripcion rapida.

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE373444C (de) 1923-04-12 Otto Ulrich Handstaubsauger
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
DE68926504T2 (de) 1988-07-20 1996-09-12 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
US5035996A (en) 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
EP0439182B1 (en) 1990-01-26 1996-04-24 Abbott Laboratories Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
EP0557456A4 (en) 1990-11-14 1995-11-15 Siska Diagnostics Inc Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor
US5386024A (en) 1993-02-10 1995-01-31 Gen-Probe Incorporated Method to prepare nucleic acids from a biological sample using low pH and acid protease
US5968729A (en) * 1994-06-10 1999-10-19 Kosak; Kenneth M. Use of centrifugation to prepare a retractable seal over reagents in a reaction container
NZ333135A (en) 1996-06-04 2000-02-28 Univ Utah Res Found Systems and methods for monitoring for DNA amplification by fluorescence using acceptor fluorophore labeled nucleic acid probe and a donor fluorophore labeled nucleic acid probe which hybridise to adjacent regions of a target sequence
DK1179600T3 (da) 1996-06-04 2005-09-05 Univ Utah Res Found Overvågning af hybridisering under PCR
US5981235A (en) 1996-07-29 1999-11-09 Promega Corporation Methods for isolating nucleic acids using alkaline protease
US6261770B1 (en) * 1997-05-13 2001-07-17 Display Systems Biotech Aps Method to clone mRNAs
JP2002534985A (ja) * 1999-01-19 2002-10-22 ソマロジック・インコーポレーテッド 核酸リガンドの自動化生成のための方法および装置
DK1232502T3 (da) 1999-11-17 2006-05-22 Roche Diagnostics Gmbh Magnetiske glaspartikler, fremgangsmåde til deres fremstilling samt anvendelser deraf
ATE384731T1 (de) 2000-08-03 2008-02-15 Hoffmann La Roche Nukleinsäurebindende verbindungen mit pyrazolo 3, 4-d pyrimidinanalogen von purin-2,6-diamin und ihre verwendung
ES2305023T3 (es) 2000-10-31 2008-11-01 F. Hoffmann-La Roche Ag Metodos para el analisis de componentes no proteinaceos usando una proteasa de una cepa de bacilo.
US8962293B2 (en) * 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
US8895240B2 (en) * 2008-05-19 2014-11-25 E I Du Pont De Nemours And Company Method, kit and system for collecting and processing samples for DNA and RNA detection
ES2439951T3 (es) * 2008-06-06 2014-01-27 F. Hoffmann-La Roche Ag Detección y cuantificación multiplex de ácidos nucleicos microbianos controlada de forma interna

Also Published As

Publication number Publication date
CA2802550C (en) 2019-01-15
CA2802550A1 (en) 2012-02-02
WO2012013732A1 (en) 2012-02-02
EP2598653B1 (en) 2017-08-23
CN103270171B (zh) 2014-09-17
ES2644797T3 (es) 2017-11-30
CN103270171A (zh) 2013-08-28
EP2598653A1 (en) 2013-06-05
JP2013542713A (ja) 2013-11-28

Similar Documents

Publication Publication Date Title
JP5871600B2 (ja) 対照核酸用一般マトリックス
JP6606138B2 (ja) 多数のパラメーターのための対照核酸
JP6169489B2 (ja) 一般的な試料調製
US9234250B2 (en) Control nucleic acids for multiple parameters
JP5972265B2 (ja) ジェネリックｐｃｒ
US20160230208A1 (en) Generic PCR
JP2019500017A (ja) ウイルスＤＮＡの選択的増幅のためのＲＮａｓｅ Ｈの使用
JP6754874B2 (ja) 多数のパラメーターのための対照核酸
JP6348202B2 (ja) 一般的な試料調製

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140401

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20140401

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150616

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150914

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20151216

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160315

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20160706

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20160712

R150 Certificate of patent or registration of utility model

Ref document number: 5972265

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250