JP2013521762A5 - - Google Patents
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- JP2013521762A5 JP2013521762A5 JP2012542287A JP2012542287A JP2013521762A5 JP 2013521762 A5 JP2013521762 A5 JP 2013521762A5 JP 2012542287 A JP2012542287 A JP 2012542287A JP 2012542287 A JP2012542287 A JP 2012542287A JP 2013521762 A5 JP2013521762 A5 JP 2013521762A5
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- JP
- Japan
- Prior art keywords
- cells
- mesoderm
- culture
- hematopoietic
- producing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 210000001704 mesoblast Anatomy 0.000 claims 6
- 210000003958 Hematopoietic Stem Cells Anatomy 0.000 claims 4
- 210000004027 cells Anatomy 0.000 claims 4
- 101700071614 WNT3A Proteins 0.000 claims 3
- 102100015886 BMP4 Human genes 0.000 claims 2
- 101700005069 BMP4 Proteins 0.000 claims 2
- 210000003743 Erythrocytes Anatomy 0.000 claims 2
- 210000003593 Megakaryocytes Anatomy 0.000 claims 2
- 210000000066 Myeloid Cells Anatomy 0.000 claims 2
- 210000000440 Neutrophils Anatomy 0.000 claims 2
- 238000004115 adherent culture Methods 0.000 claims 2
- 230000003394 haemopoietic Effects 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 210000001778 pluripotent stem cell Anatomy 0.000 claims 2
- 102100016492 CD34 Human genes 0.000 claims 1
- 108060001251 CD34 Proteins 0.000 claims 1
- 101700084039 EPO Proteins 0.000 claims 1
- 101710042422 EPX Proteins 0.000 claims 1
- 101710022257 FLT3LG Proteins 0.000 claims 1
- 102100004572 FLT3LG Human genes 0.000 claims 1
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 claims 1
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 claims 1
- 108010002386 Interleukin-3 Proteins 0.000 claims 1
- 101710028765 KITLG Proteins 0.000 claims 1
- 101710040065 THPO Proteins 0.000 claims 1
- 101700040544 TIMP1 Proteins 0.000 claims 1
- 101700076107 TPO Proteins 0.000 claims 1
- 229920002397 Thermoplastic olefin Polymers 0.000 claims 1
- 102100015249 VEGFA Human genes 0.000 claims 1
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 claims 1
- 230000001070 adhesive Effects 0.000 claims 1
- 239000000853 adhesive Substances 0.000 claims 1
- 238000003501 co-culture Methods 0.000 claims 1
- 108010082117 matrigel Proteins 0.000 claims 1
Claims (10)
- 以下の培養工程により、多能性幹細胞を異種細胞と共培養することなく培養して中胚葉細胞を得ることを特徴とする、中胚葉細胞の製造方法；
（１）BMP4を含み、Wnt3aを含まない無血清培地で多能性幹細胞を接着培養する工程、
（２）工程（１）で得られた細胞をBMP4およびWnt3aを含む無血清培地で接着培養する工程、および
（３）工程（２）で得られた細胞をVEGFおよびWnt3aを含む無血清培地で接着培養する工程。 - 前記接着培養は、Matrigel（商標）でコートされた培養器で行われる、請求項１に記載の方法。
- 前記工程（１）が１日間行われる、請求項１または２に記載の方法。
- 前記工程（２）が３日間行われる、請求項１から３のいずれか１項に記載の方法。
- 前記工程（３）が２日間行われる、請求項１から４のいずれか１項に記載の方法。
- 前記中胚葉細胞が、KDR陽性およびCD34陽性細胞を含む、請求項１から５のいずれか１項に記載の方法。
- ａ） 請求項１から６のいずれか１項に記載の方法で中胚葉細胞を製造する工程、および
ｂ） ａ）の中胚葉細胞から造血幹細胞、造血前駆細胞、骨髄系細胞、巨核球、赤血球および／または好中球を製造する工程を含む、造血幹細胞、造血前駆細胞、骨髄系細胞、巨核球、赤血球および／または好中球の製造方法。 - 前記工程ｂ）は工程ａ）で得られた中胚葉細胞を造血因子の存在下で培養することによって行われる、請求項７に記載の方法。
- 前記造血因子が、SCF、TPO、EPO、IL-3、 Flt3-ligand、FP-6およびG-CSFからなる群より選ばれる、請求項８に記載の方法。
- 前記工程（ｂ）が９日以上の間行われる、請求項７から９のいずれか１項に記載の方法。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US31517010P | 2010-03-18 | 2010-03-18 | |
| US61/315,170 | 2010-03-18 | ||
| PCT/JP2011/057503 WO2011115308A1 (en) | 2010-03-18 | 2011-03-18 | Method for inducing differentiation of pluripotent stem cells into mesodermal cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013521762A JP2013521762A (ja) | 2013-06-13 |
| JP2013521762A5 true JP2013521762A5 (ja) | 2014-06-19 |
| JP5777115B2 JP5777115B2 (ja) | 2015-09-09 |
Family
ID=44649386
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012542287A Expired - Fee Related JP5777115B2 (ja) | 2010-03-18 | 2011-03-18 | 多能性幹細胞から中胚葉細胞への分化誘導法 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US8652845B2 (ja) |
| EP (1) | EP2547764B1 (ja) |
| JP (1) | JP5777115B2 (ja) |
| WO (1) | WO2011115308A1 (ja) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9074186B2 (en) * | 2012-08-15 | 2015-07-07 | Boston Medical Center Corporation | Production of red blood cells and platelets from stem cells |
| US9763984B2 (en) * | 2012-12-21 | 2017-09-19 | Astellas Institute For Regenerative Medicine | Methods for production of platelets from pluripotent stem cells and compositions thereof |
| US10240126B2 (en) | 2013-06-12 | 2019-03-26 | Kyoto University | Induced pluripotent stem cell selection method and method for inducing differentiation to blood cells |
| FR3024462A1 (fr) * | 2014-07-29 | 2016-02-05 | Univ Pierre Et Marie Curie Paris 6 | Procede de production in vitro de progeniteurs adipocytaires et d'adipocytes |
| CA2968655C (en) | 2014-11-25 | 2021-11-16 | President And Fellows Of Harvard College | Methods for generation of podocytes from pluripotent stem cells and cells produced by the same |
| US10626372B1 (en) * | 2015-01-26 | 2020-04-21 | Fate Therapeutics, Inc. | Methods and compositions for inducing hematopoietic cell differentiation |
| CN108138129B (zh) * | 2015-07-17 | 2021-11-16 | 国立大学法人京都大学 | 血管内皮细胞的诱导方法 |
| JP6646311B2 (ja) * | 2015-07-17 | 2020-02-14 | 国立大学法人京都大学 | 多能性幹細胞から中胚葉前駆細胞および血液血管前駆細胞への分化誘導法 |
| CN108368520B (zh) | 2015-11-04 | 2023-01-17 | 菲特治疗公司 | 多能细胞的基因组工程改造 |
| US10858628B2 (en) * | 2015-11-04 | 2020-12-08 | Fate Therapeutics, Inc. | Methods and compositions for inducing hematopoietic cell differentiation |
| WO2017164257A1 (ja) * | 2016-03-23 | 2017-09-28 | 国立大学法人京都大学 | 血球分化能の高い中胚葉誘導方法 |
| JP7171055B2 (ja) | 2017-03-14 | 2022-11-15 | 国立大学法人京都大学 | 多能性幹細胞からヘルパーｔ細胞を製造する方法 |
| EP3901253A4 (en) * | 2018-12-20 | 2022-09-07 | Sumitomo Chemical Company Limited | EMBRYONIC ERYTHROBLAST CONTAINING CELL POPULATION AND METHODS FOR THEIR PRODUCTION, CELL CULTURE COMPOSITION AND COMBINATION TESTING METHODS |
| GB201911957D0 (en) * | 2019-08-20 | 2019-10-02 | Adaptimmune Ltd | Methods of producing haemogenic progenitor cells from pluripotent stem cells |
| WO2021107117A1 (ja) * | 2019-11-28 | 2021-06-03 | 国立大学法人京都大学 | 多能性幹細胞からの造血細胞の製造法 |
| JP7072756B2 (ja) * | 2019-12-27 | 2022-05-23 | 国立大学法人京都大学 | 多能性幹細胞から中胚葉前駆細胞および血液血管前駆細胞への分化誘導法 |
| EP4110900A4 (en) * | 2020-02-28 | 2024-04-24 | Purdue Research Foundation | GENERATING AORTA-GONAD-MESONEPHROS-LIKE HEMATOPOETIC CELLS FROM HUMAN PLURIPOTENT STEM CELLS UNDER A DEFINED STATE |
| JP7409203B2 (ja) | 2020-04-02 | 2024-01-09 | Ｔｏｐｐａｎホールディングス株式会社 | 紙箱 |
| US11591381B2 (en) | 2020-11-30 | 2023-02-28 | Crispr Therapeutics Ag | Gene-edited natural killer cells |
| CN112574944A (zh) * | 2020-12-14 | 2021-03-30 | 广州医科大学附属第三医院（广州重症孕产妇救治中心、广州柔济医院） | 基于体外诱导eps发育形成类囊胚结构的方法 |
| WO2022144632A1 (en) | 2020-12-30 | 2022-07-07 | Crispr Therapeutics Ag | Compositions and methods for differentiating stem cells into nk cells |
| GB202102297D0 (en) * | 2021-02-18 | 2021-04-07 | Adaptimmune Ltd | Methods of Producing Haemogenic Endothelial Cells from Pluripotent Stem Cells |
| CN113249303B (zh) * | 2021-05-07 | 2022-07-29 | 华中农业大学 | 血管内皮细胞生长因子在促进鸡原始生殖细胞增殖和迁移中的应用 |
| CN114457006B (zh) * | 2022-02-21 | 2023-07-28 | 广州华越肾科再生医学科技有限公司 | 一种通过不连续分化制备肾脏足细胞的方法 |
| CN115247152B (zh) * | 2022-09-21 | 2022-12-27 | 呈诺再生医学科技(北京)有限公司 | 制备造血干细胞或造血干祖细胞的方法及培养长期再生造血干细胞的方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6667176B1 (en) * | 2000-01-11 | 2003-12-23 | Geron Corporation | cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells |
| MXPA04005010A (es) * | 2001-11-26 | 2005-04-08 | Advanced Cell Tech Inc | METODO PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS METODOS PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS HUMANOS REPROGRAMADOS Y CELULAS DEL TALLO HUMANAS AUTOLOGAS E ISOGENICAS. |
| US7452718B2 (en) * | 2004-03-26 | 2008-11-18 | Geron Corporation | Direct differentiation method for making cardiomyocytes from human embryonic stem cells |
| EP2377924A1 (en) | 2006-04-14 | 2011-10-19 | Advanced Cell Technology, Inc. | Hemangio-colony forming cells |
| WO2008056779A1 (fr) | 2006-11-09 | 2008-05-15 | Japan As Represented By The President Of International Medical Center Of Japan | Procédé destiné à la culture et au passage d'une cellule souche embryonnaire de primate, et procédé destiné à induire la différenciation de la cellule souche embryonnaire |
| WO2009116951A2 (en) * | 2008-03-17 | 2009-09-24 | Agency For Science, Technology And Research | Microcarriers for stem cell culture |
| EP2268794B1 (en) * | 2008-03-27 | 2017-09-13 | Asterias Biotherapeutics, Inc. | Differentiation of primate pluripotent stem cells to hematopoietic lineage cells |
| JPWO2010008100A1 (ja) | 2008-07-17 | 2012-01-05 | 国立大学法人名古屋大学 | 骨格筋細胞又は骨細胞の誘導法 |
-
2011
- 2011-03-18 WO PCT/JP2011/057503 patent/WO2011115308A1/en active Application Filing
- 2011-03-18 JP JP2012542287A patent/JP5777115B2/ja not_active Expired - Fee Related
- 2011-03-18 US US13/635,679 patent/US8652845B2/en not_active Expired - Fee Related
- 2011-03-18 EP EP11756490.6A patent/EP2547764B1/en active Active
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