JP2013511292A - プローブハイブリダイゼーションおよび制限を使用したシグナル増幅による標的核酸のアッセイ方法 - Google Patents

プローブハイブリダイゼーションおよび制限を使用したシグナル増幅による標的核酸のアッセイ方法 Download PDF

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JP2013511292A
JP2013511292A JP2012541159A JP2012541159A JP2013511292A JP 2013511292 A JP2013511292 A JP 2013511292A JP 2012541159 A JP2012541159 A JP 2012541159A JP 2012541159 A JP2012541159 A JP 2012541159A JP 2013511292 A JP2013511292 A JP 2013511292A
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sequence
oligonucleotide probe
probe
nucleic acid
oligonucleotide
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JP2013511292A5 (enExample
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ナデュー ジム
ヘルヤー トービン
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Becton Dickinson and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2012541159A 2009-11-23 2010-11-23 プローブハイブリダイゼーションおよび制限を使用したシグナル増幅による標的核酸のアッセイ方法 Withdrawn JP2013511292A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US26359609P 2009-11-23 2009-11-23
US61/263,596 2009-11-23
PCT/US2010/057781 WO2011063388A2 (en) 2009-11-23 2010-11-23 Assay method for target nucleic acid by signal amplification using probe hybridization and restriction

Publications (2)

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JP2013511292A true JP2013511292A (ja) 2013-04-04
JP2013511292A5 JP2013511292A5 (enExample) 2014-01-23

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JP2012541159A Withdrawn JP2013511292A (ja) 2009-11-23 2010-11-23 プローブハイブリダイゼーションおよび制限を使用したシグナル増幅による標的核酸のアッセイ方法

Country Status (5)

Country Link
US (1) US8916349B2 (enExample)
EP (1) EP2504453A4 (enExample)
JP (1) JP2013511292A (enExample)
CN (1) CN102686747A (enExample)
WO (1) WO2011063388A2 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018526009A (ja) * 2015-09-03 2018-09-13 ナノストリング テクノロジーズ，インコーポレイティド ヌクレオチド１個の分解能を有する多価プローブ
JP2019532632A (ja) * 2016-09-20 2019-11-14 ベース４ イノベーション リミテッド 単一ヌクレオチド検出方法および関連プローブ
JP2020521450A (ja) * 2017-05-23 2020-07-27 ラトガース，ザ ステート ユニバーシティ オブ ニュー ジャージー デュアル相互作用ヘアピンプローブを使用した標的媒介ｉｎ ｓｉｔｕシグナル増幅

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WO2011022820A1 (en) 2009-08-31 2011-03-03 The Governors Of The University Of Alberta Binding-induced hairpin detection system
US9603949B2 (en) 2011-09-01 2017-03-28 University Of Iowa Research Foundation Oligonucleotide-based probes for detection of bacterial nucleases
SG11201404899VA (en) 2012-02-14 2014-10-30 Univ Cornell Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions
GB201217770D0 (en) * 2012-10-04 2012-11-14 Base4 Innovation Ltd Biological probes and the use thereof
GB201310823D0 (en) * 2013-06-18 2013-07-31 Lgc Ltd Fluorophore-based oligonucleotide probes with a universal element
GB2520763A (en) * 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Nucleic acid probe and method of detecting genomic fragments
CA2939124C (en) 2014-02-07 2023-08-29 University Of Iowa Research Foundation Oligonucleotide-based probes and methods for detection of microbes
EP3158082B1 (en) 2014-06-19 2020-12-30 Multiplicom NV Restriction mediated quantitative polymerase chain reactions
KR102465287B1 (ko) * 2014-08-11 2022-11-09 루미넥스 코포레이션 핵산 검정에서 개선된 용융 판별 및 멀티플렉싱을 위한 프로브
US20180179576A1 (en) * 2015-03-02 2018-06-28 The Regents Of The University Of California Homogeneous entropy-driven biomolecular assay (heba)
ITUB20155686A1 (it) * 2015-11-18 2017-05-18 Fondazione St Italiano Tecnologia Procedimento di rivelazione di acidi nucleici e relativo kit.
ITUB20155706A1 (it) * 2015-11-18 2017-05-18 Fondazione St Italiano Tecnologia Procedimento di rivelazione di acidi nucleici e relativo kit.
US20190023675A1 (en) * 2017-07-20 2019-01-24 Eastman Chemical Company Method for producing purified dialkyl-furan-2,5-dicarboxylate
JP7421202B2 (ja) 2017-09-29 2024-01-24 ユニバーシティー オブ アイオワ リサーチ ファンデーション デジタルヌクレアーゼ検出組成物および方法
US20230212702A1 (en) * 2020-06-04 2023-07-06 Logicink Corporation Multi-sensing of nucleic acid and small molecule markers
CN111944928B (zh) * 2020-09-01 2024-05-31 湖南工程学院 基于三链dna结构的多维立体网状杂交链式信号放大的检测eb病毒的序列组合和方法
CN114002200B (zh) * 2021-11-01 2024-05-07 中国科学院苏州纳米技术与纳米仿生研究所 近红外二区激活型探针及其应用
JP2025531879A (ja) * 2022-09-08 2025-09-25 アドヴァンスド セル ダイアグノスティックス インコーポレイテッド Ｒｎａ遮断分子を使用した標的核酸の検出方法

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US4766062A (en) 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US5424413A (en) * 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5846726A (en) 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US5935791A (en) 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
DE1051515T1 (de) * 1998-01-27 2001-04-19 Cytocell Ltd., Banbury Modifizierte nuklein-säure sonden und dessen verwendungen
US6316200B1 (en) 2000-06-08 2001-11-13 Becton, Dickinson And Company Probes and methods for detection of nucleic acids
CA2392469A1 (en) 1999-11-29 2001-05-31 Gamida Sense Diagnostics Ltd. Oligonucleotides and assemblies thereof useful in the detection of the presence or absence of target nucleic acid sequences in a sample
US7361469B2 (en) 2004-08-13 2008-04-22 Stratagene California Dual labeled fluorescent probes
JP2007075023A (ja) 2005-09-15 2007-03-29 Shimadzu Corp 蛍光共鳴エネルギー移動法を用いた遺伝子多型検出法及びキット
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CN101215600B (zh) 2007-01-04 2012-08-15 中国科学院广州生物医药与健康研究院 核酸目的序列的检测探针及检测方法
WO2009055366A1 (en) 2007-10-22 2009-04-30 Translational Genomics Research Institute (Tgen) Method and kit used in performing high resolution genomic profiling of clonal cell populations in heterogeneous tissues
CN101250593B (zh) 2008-02-03 2011-01-26 广州益善生物技术有限公司 乳头瘤病毒检测与分型方法及其液相芯片
CN101328498B (zh) 2008-06-11 2010-10-27 北京大学 一种通用、实时的核酸酶荧光检测方法
WO2010030716A1 (en) 2008-09-10 2010-03-18 Igor Kutyavin Detection of nucleic acids by oligonucleotide probes cleaved in presence of endonuclease v
CA2755615A1 (en) * 2009-03-18 2010-09-23 Sequenom, Inc. Use of thermostable endonucleases for generating reporter molecules

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018526009A (ja) * 2015-09-03 2018-09-13 ナノストリング テクノロジーズ，インコーポレイティド ヌクレオチド１個の分解能を有する多価プローブ
JP2019532632A (ja) * 2016-09-20 2019-11-14 ベース４ イノベーション リミテッド 単一ヌクレオチド検出方法および関連プローブ
JP2020521450A (ja) * 2017-05-23 2020-07-27 ラトガース，ザ ステート ユニバーシティ オブ ニュー ジャージー デュアル相互作用ヘアピンプローブを使用した標的媒介ｉｎ ｓｉｔｕシグナル増幅
US11459603B2 (en) 2017-05-23 2022-10-04 Rutgers, The State University Of New Jersey Target mediated in situ signal amplification with dual interacting hairpin probes
JP7240733B2 (ja) 2017-05-23 2023-03-16 ラトガース，ザ ステート ユニバーシティ オブ ニュー ジャージー デュアル相互作用ヘアピンプローブを使用した標的媒介ｉｎ ｓｉｔｕシグナル増幅

Also Published As

Publication number Publication date
WO2011063388A3 (en) 2011-10-20
US20120322067A1 (en) 2012-12-20
WO2011063388A2 (en) 2011-05-26
CN102686747A (zh) 2012-09-19
US8916349B2 (en) 2014-12-23
EP2504453A4 (en) 2013-07-17
EP2504453A2 (en) 2012-10-03

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