JP2013507922A - 細胞培養用マイクロ流体デバイス - Google Patents
細胞培養用マイクロ流体デバイス Download PDFInfo
- Publication number
- JP2013507922A JP2013507922A JP2012534269A JP2012534269A JP2013507922A JP 2013507922 A JP2013507922 A JP 2013507922A JP 2012534269 A JP2012534269 A JP 2012534269A JP 2012534269 A JP2012534269 A JP 2012534269A JP 2013507922 A JP2013507922 A JP 2013507922A
- Authority
- JP
- Japan
- Prior art keywords
- cell
- cells
- chamber
- cell culture
- trough
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 73
- 230000010412 perfusion Effects 0.000 claims abstract description 120
- 239000012530 fluid Substances 0.000 claims abstract description 67
- 238000004891 communication Methods 0.000 claims abstract description 28
- 230000006854 communication Effects 0.000 claims abstract description 28
- 210000004027 cell Anatomy 0.000 claims description 338
- 239000006143 cell culture medium Substances 0.000 claims description 25
- 210000004748 cultured cell Anatomy 0.000 claims description 14
- 230000014759 maintenance of location Effects 0.000 abstract description 20
- 210000003494 hepatocyte Anatomy 0.000 description 53
- 238000000034 method Methods 0.000 description 37
- 239000000126 substance Substances 0.000 description 23
- 230000006870 function Effects 0.000 description 22
- 230000000694 effects Effects 0.000 description 19
- 229910052710 silicon Inorganic materials 0.000 description 18
- 239000010703 silicon Substances 0.000 description 18
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 17
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 17
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 17
- 210000002744 extracellular matrix Anatomy 0.000 description 17
- 239000000463 material Substances 0.000 description 16
- 230000032258 transport Effects 0.000 description 14
- 239000004205 dimethyl polysiloxane Substances 0.000 description 13
- 238000001727 in vivo Methods 0.000 description 13
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 13
- 238000011534 incubation Methods 0.000 description 12
- 239000000203 mixture Substances 0.000 description 12
- 229920002120 photoresistant polymer Polymers 0.000 description 11
- 239000000758 substrate Substances 0.000 description 10
- 238000012258 culturing Methods 0.000 description 9
- 108090000623 proteins and genes Proteins 0.000 description 9
- 102000004169 proteins and genes Human genes 0.000 description 9
- 238000002073 fluorescence micrograph Methods 0.000 description 8
- 235000015097 nutrients Nutrition 0.000 description 8
- 210000000013 bile duct Anatomy 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 7
- 230000007774 longterm Effects 0.000 description 7
- 238000010186 staining Methods 0.000 description 7
- 230000003068 static effect Effects 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- 238000009792 diffusion process Methods 0.000 description 6
- 238000000338 in vitro Methods 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 239000012528 membrane Substances 0.000 description 6
- -1 polydimethylsiloxane Polymers 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 5
- 238000013461 design Methods 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 239000002207 metabolite Substances 0.000 description 5
- 230000003278 mimic effect Effects 0.000 description 5
- 239000001301 oxygen Substances 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000002699 waste material Substances 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 230000004888 barrier function Effects 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 239000000701 coagulant Substances 0.000 description 4
- 238000000576 coating method Methods 0.000 description 4
- 229920001577 copolymer Polymers 0.000 description 4
- 230000036267 drug metabolism Effects 0.000 description 4
- 230000001788 irregular Effects 0.000 description 4
- 238000010899 nucleation Methods 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 108010035532 Collagen Proteins 0.000 description 3
- 102000008186 Collagen Human genes 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 229920001436 collagen Polymers 0.000 description 3
- 229940000406 drug candidate Drugs 0.000 description 3
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 230000008611 intercellular interaction Effects 0.000 description 3
- 239000010410 layer Substances 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 230000008520 organization Effects 0.000 description 3
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 239000004926 polymethyl methacrylate Substances 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 230000002441 reversible effect Effects 0.000 description 3
- 150000004756 silanes Chemical class 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- 230000001988 toxicity Effects 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- CHADEQDQBURGHL-UHFFFAOYSA-N (6'-acetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(OC(C)=O)C=C1OC1=CC(OC(=O)C)=CC=C21 CHADEQDQBURGHL-UHFFFAOYSA-N 0.000 description 2
- IOOMXAQUNPWDLL-UHFFFAOYSA-N 2-[6-(diethylamino)-3-(diethyliminiumyl)-3h-xanthen-9-yl]-5-sulfobenzene-1-sulfonate Chemical compound C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=C(S(O)(=O)=O)C=C1S([O-])(=O)=O IOOMXAQUNPWDLL-UHFFFAOYSA-N 0.000 description 2
- 238000012605 2D cell culture Methods 0.000 description 2
- 238000012604 3D cell culture Methods 0.000 description 2
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 108010078791 Carrier Proteins Proteins 0.000 description 2
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 2
- 229920000089 Cyclic olefin copolymer Polymers 0.000 description 2
- 102100037260 Gap junction beta-1 protein Human genes 0.000 description 2
- XKLMZUWKNUAPSZ-UHFFFAOYSA-N N-(2,6-dimethylphenyl)-2-{4-[2-hydroxy-3-(2-methoxyphenoxy)propyl]piperazin-1-yl}acetamide Chemical compound COC1=CC=CC=C1OCC(O)CN1CCN(CC(=O)NC=2C(=CC=CC=2C)C)CC1 XKLMZUWKNUAPSZ-UHFFFAOYSA-N 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 229920000954 Polyglycolide Polymers 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 229940072056 alginate Drugs 0.000 description 2
- 229920000615 alginic acid Polymers 0.000 description 2
- 235000010443 alginic acid Nutrition 0.000 description 2
- 230000003915 cell function Effects 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 230000036755 cellular response Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 108010015416 connexin 32 Proteins 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000008406 drug-drug interaction Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 108010082117 matrigel Proteins 0.000 description 2
- 238000004264 monolayer culture Methods 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 238000000206 photolithography Methods 0.000 description 2
- 229920000747 poly(lactic acid) Polymers 0.000 description 2
- 239000004633 polyglycolic acid Substances 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 229960000213 ranolazine Drugs 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000011664 signaling Effects 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- PYJJCSYBSYXGQQ-UHFFFAOYSA-N trichloro(octadecyl)silane Chemical compound CCCCCCCCCCCCCCCCCC[Si](Cl)(Cl)Cl PYJJCSYBSYXGQQ-UHFFFAOYSA-N 0.000 description 2
- OMDQUFIYNPYJFM-XKDAHURESA-N (2r,3r,4s,5r,6s)-2-(hydroxymethyl)-6-[[(2r,3s,4r,5s,6r)-4,5,6-trihydroxy-3-[(2s,3s,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]methoxy]oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)[C@H](O)[C@H](O)[C@H](O)O1 OMDQUFIYNPYJFM-XKDAHURESA-N 0.000 description 1
- BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 description 1
- AEMOLEFTQBMNLQ-VANFPWTGSA-N D-mannopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H]1O AEMOLEFTQBMNLQ-VANFPWTGSA-N 0.000 description 1
- 238000000116 DAPI staining Methods 0.000 description 1
- 102000016359 Fibronectins Human genes 0.000 description 1
- 108010067306 Fibronectins Proteins 0.000 description 1
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 229920000926 Galactomannan Polymers 0.000 description 1
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 241000233805 Phoenix Species 0.000 description 1
- 229920002873 Polyethylenimine Polymers 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 108010067787 Proteoglycans Proteins 0.000 description 1
- 102000016611 Proteoglycans Human genes 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 229920000147 Styrene maleic anhydride Polymers 0.000 description 1
- 102000000591 Tight Junction Proteins Human genes 0.000 description 1
- 108010002321 Tight Junction Proteins Proteins 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- WDJHALXBUFZDSR-UHFFFAOYSA-N acetoacetic acid Chemical compound CC(=O)CC(O)=O WDJHALXBUFZDSR-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009056 active transport Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 229940061720 alpha hydroxy acid Drugs 0.000 description 1
- 150000001280 alpha hydroxy acids Chemical class 0.000 description 1
- WYTGDNHDOZPMIW-RCBQFDQVSA-N alstonine Natural products C1=CC2=C3C=CC=CC3=NC2=C2N1C[C@H]1[C@H](C)OC=C(C(=O)OC)[C@H]1C2 WYTGDNHDOZPMIW-RCBQFDQVSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- AEMOLEFTQBMNLQ-UHFFFAOYSA-N beta-D-galactopyranuronic acid Natural products OC1OC(C(O)=O)C(O)C(O)C1O AEMOLEFTQBMNLQ-UHFFFAOYSA-N 0.000 description 1
- 239000003833 bile salt Substances 0.000 description 1
- 229920002988 biodegradable polymer Polymers 0.000 description 1
- 239000004621 biodegradable polymer Substances 0.000 description 1
- 239000003124 biologic agent Substances 0.000 description 1
- 230000001925 catabolic effect Effects 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 108010015046 cell aggregation factors Proteins 0.000 description 1
- 238000000423 cell based assay Methods 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 230000008614 cellular interaction Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007665 chronic toxicity Effects 0.000 description 1
- 231100000160 chronic toxicity Toxicity 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000003501 co-culture Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000003851 corona treatment Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 239000000412 dendrimer Substances 0.000 description 1
- 229920000736 dendritic polymer Polymers 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 239000002359 drug metabolite Substances 0.000 description 1
- 238000003255 drug test Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000000609 electron-beam lithography Methods 0.000 description 1
- 238000004049 embossing Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000034964 establishment of cell polarity Effects 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- KRWWZDVIEFSIOT-UHFFFAOYSA-N ethenyl acetate;furan-2,5-dione Chemical compound CC(=O)OC=C.O=C1OC(=O)C=C1 KRWWZDVIEFSIOT-UHFFFAOYSA-N 0.000 description 1
- 229920001038 ethylene copolymer Polymers 0.000 description 1
- 210000003722 extracellular fluid Anatomy 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 229920002313 fluoropolymer Polymers 0.000 description 1
- 238000002825 functional assay Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000002064 heart cell Anatomy 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000012744 immunostaining Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000010874 in vitro model Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 102000006495 integrins Human genes 0.000 description 1
- 108010044426 integrins Proteins 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 210000004692 intercellular junction Anatomy 0.000 description 1
- 230000035990 intercellular signaling Effects 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 229960000448 lactic acid Drugs 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 210000005228 liver tissue Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000007102 metabolic function Effects 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 238000005459 micromachining Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 239000012120 mounting media Substances 0.000 description 1
- 210000004498 neuroglial cell Anatomy 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- JFNLZVQOOSMTJK-KNVOCYPGSA-N norbornene Chemical compound C1[C@@H]2CC[C@H]1C=C2 JFNLZVQOOSMTJK-KNVOCYPGSA-N 0.000 description 1
- 229920000620 organic polymer Polymers 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 210000004738 parenchymal cell Anatomy 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 238000009832 plasma treatment Methods 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000007423 screening assay Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- 150000003376 silicon Chemical class 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 238000005549 size reduction Methods 0.000 description 1
- 229940126586 small molecule drug Drugs 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000807 solvent casting Methods 0.000 description 1
- 238000004528 spin coating Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 238000004381 surface treatment Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 230000002277 temperature effect Effects 0.000 description 1
- 229940124598 therapeutic candidate Drugs 0.000 description 1
- 229920001169 thermoplastic Polymers 0.000 description 1
- 210000001578 tight junction Anatomy 0.000 description 1
- 238000012876 topography Methods 0.000 description 1
- 231100000041 toxicology testing Toxicity 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 239000006163 transport media Substances 0.000 description 1
- AVYKQOAMZCAHRG-UHFFFAOYSA-N triethoxy(3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl)silane Chemical compound CCO[Si](OCC)(OCC)CCC(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)F AVYKQOAMZCAHRG-UHFFFAOYSA-N 0.000 description 1
- BVQYIDJXNYHKRK-UHFFFAOYSA-N trimethoxy(3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl)silane Chemical compound CO[Si](OC)(OC)CCC(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)F BVQYIDJXNYHKRK-UHFFFAOYSA-N 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/24—Gas permeable parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/40—Manifolds; Distribution pieces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Dispersion Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25075409P | 2009-10-12 | 2009-10-12 | |
| US61/250,754 | 2009-10-12 | ||
| PCT/US2010/052212 WO2011046884A2 (en) | 2009-10-12 | 2010-10-12 | Microfluidic device for cell culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2013507922A true JP2013507922A (ja) | 2013-03-07 |
| JP2013507922A5 JP2013507922A5 (https=) | 2013-05-30 |
Family
ID=43855151
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012534269A Abandoned JP2013507922A (ja) | 2009-10-12 | 2010-10-12 | 細胞培養用マイクロ流体デバイス |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US8481303B2 (https=) |
| EP (1) | EP2488628A2 (https=) |
| JP (1) | JP2013507922A (https=) |
| CN (1) | CN102612554A (https=) |
| WO (1) | WO2011046884A2 (https=) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101979145B1 (ko) * | 2018-01-30 | 2019-05-15 | 서강대학교산학협력단 | 신경줄기세포 배양용 미세유체칩 및 이의 용도 |
| JP2019513409A (ja) * | 2016-02-16 | 2019-05-30 | マテリオミクス ベーフェー | 生細胞の生理機能を変化させるための表面トポグラフィ |
| JP2019513416A (ja) * | 2016-03-29 | 2019-05-30 | エスエムエスバイオテック インコーポレイテッドＳｍｓｂｉｏｔｅｃｈ，Ｉｎｃ． | 小型運動性幹細胞を使用した組成物および方法 |
| KR20190130627A (ko) * | 2017-03-31 | 2019-11-22 | 다나-파버 캔서 인스티튜트 인크. | ３d 미세유체 세포 배양 장치를 사용하여 종양 세포 구상체를 평가하는 방법 |
| WO2020101242A1 (ko) * | 2018-11-13 | 2020-05-22 | 엠비디 주식회사 | 세포 배양 플레이트 |
Families Citing this family (56)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7534601B2 (en) * | 2002-08-27 | 2009-05-19 | Vanderbilt University | Capillary perfused bioreactors with multiple chambers |
| EP2358860A1 (en) * | 2008-11-21 | 2011-08-24 | Corning Incorporated | Spaced projection substrates and devices for cell culture |
| JP5879267B2 (ja) * | 2009-10-29 | 2016-03-08 | ザ・チャールズ・スターク・ドレイパ・ラボラトリー・インコーポレイテッド | 血液透析用マイクロ流体装置 |
| US9522396B2 (en) | 2010-12-29 | 2016-12-20 | S.D. Sight Diagnostics Ltd. | Apparatus and method for automatic detection of pathogens |
| CN102234614B (zh) * | 2011-05-30 | 2013-02-13 | 中国科学院苏州纳米技术与纳米仿生研究所 | 微流控细胞悬浮培养芯片及其应用 |
| KR101970634B1 (ko) | 2011-06-02 | 2019-04-19 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 생체외 조직 배양 시스템을 위한 방법 및 용도 |
| WO2012174460A1 (en) * | 2011-06-15 | 2012-12-20 | The Charles Stark Draper Laboratory, Inc. | Systems, methods, and devices relating to a biomimetic cellularized nephron unit |
| JP2014516582A (ja) * | 2011-06-15 | 2014-07-17 | ザ チャールズ スターク ドレイパー ラボラトリー インク | 生体模倣環境において細胞を培養するためのデバイスおよび方法 |
| US10640807B2 (en) | 2011-12-29 | 2020-05-05 | S.D. Sight Diagnostics Ltd | Methods and systems for detecting a pathogen in a biological sample |
| WO2014031523A2 (en) * | 2012-08-19 | 2014-02-27 | University Of Rochester | Method for preparing microfluidic device with reduced channel height |
| US20140127733A1 (en) * | 2012-11-01 | 2014-05-08 | Moffit Cancer Center | Ex vivo microfluidic analysis of biologic samples |
| EP2972313B1 (en) | 2013-03-15 | 2018-01-03 | Rinker, Kristina, D. | Ported parallel plate flow chamber and methods for use thereof |
| EP2999988A4 (en) | 2013-05-23 | 2017-01-11 | S.D. Sight Diagnostics Ltd. | Method and system for imaging a cell sample |
| IL227276A0 (en) | 2013-07-01 | 2014-03-06 | Parasight Ltd | A method and system for preparing a monolayer of cells, particularly suitable for diagnosis |
| EP3024921B1 (en) | 2013-07-22 | 2025-12-10 | The Regents of The University of California | Microfluidics cell culture device |
| WO2015029032A1 (en) | 2013-08-26 | 2015-03-05 | Parasight Ltd. | Digital microscopy systems, methods and computer program products |
| US20150064689A1 (en) * | 2013-08-29 | 2015-03-05 | The Charles Stark Draper Laboratory, Inc. | System and method for analyzing transmissibility of influenza |
| CN103627635B (zh) * | 2013-11-18 | 2015-05-20 | 辽宁中医药大学 | 一种用于细胞迁移和侵袭实验的多功能微流控芯片 |
| US20150247112A1 (en) * | 2014-03-03 | 2015-09-03 | Kiyatec Inc. | 3D Tissue Culture Devices and Systems |
| US9631167B2 (en) * | 2014-03-21 | 2017-04-25 | Iontox, Llc | Dynamic multi organ plate |
| EP3177709B1 (en) | 2014-08-07 | 2022-05-18 | The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone | Reversible stencils for fabricating micro-tissues |
| US10482595B2 (en) | 2014-08-27 | 2019-11-19 | S.D. Sight Diagnostics Ltd. | System and method for calculating focus variation for a digital microscope |
| CN104293666B (zh) * | 2014-09-11 | 2016-06-22 | 大连理工大学 | 两种不同单细胞之间相互作用的微流控芯片装置 |
| CN105713835B (zh) * | 2014-12-05 | 2018-11-09 | 中国科学院大连化学物理研究所 | 一种基于微流控芯片的多功能区域细胞三维共培养方法 |
| US10279345B2 (en) | 2014-12-12 | 2019-05-07 | Opko Diagnostics, Llc | Fluidic systems comprising an incubation channel, including fluidic systems formed by molding |
| US10723988B2 (en) | 2015-04-22 | 2020-07-28 | Berkeley Lights, Inc. | Microfluidic cell culture |
| GB201512600D0 (en) * | 2015-07-17 | 2015-08-26 | Koniku Ltd | Cell culture, transport and investigation |
| US10488644B2 (en) | 2015-09-17 | 2019-11-26 | S.D. Sight Diagnostics Ltd. | Methods and apparatus for detecting an entity in a bodily sample |
| US10799865B2 (en) | 2015-10-27 | 2020-10-13 | Berkeley Lights, Inc. | Microfluidic apparatus having an optimized electrowetting surface and related systems and methods |
| GB2560290B (en) | 2015-12-04 | 2022-03-09 | Emulate Inc | Open-Top Microfluidic Device With Structural Anchors |
| CA3018536A1 (en) | 2016-03-30 | 2017-10-05 | S.D. Sight Diagnostics Ltd | Distinguishing between blood sample components |
| US11414635B2 (en) | 2016-04-18 | 2022-08-16 | Saint Louis University | Integration of three dimensional cell culture scaffolds in microfluidic devices by direct fiber spinning |
| EP3455626B1 (en) | 2016-05-11 | 2025-08-06 | S.D. Sight Diagnostics Ltd. | Performing optical measurements on a sample |
| WO2017195205A1 (en) | 2016-05-11 | 2017-11-16 | S.D. Sight Diagnostics Ltd | Sample carrier for optical measurements |
| IL263274B2 (en) | 2016-05-26 | 2023-10-01 | Berkeley Lights Inc | Covalently adapted surfaces, kits and methods for their production and uses |
| CN105838606B (zh) * | 2016-06-08 | 2018-10-02 | 牛海涛 | 一种高通量细胞联合培养芯片 |
| US10243097B2 (en) * | 2016-09-09 | 2019-03-26 | eLux Inc. | Fluidic assembly using tunable suspension flow |
| WO2018080308A2 (en) * | 2016-10-28 | 2018-05-03 | Materiomics B.V. | Surface topographies for stimulating endothelialization |
| US11474103B2 (en) | 2017-02-17 | 2022-10-18 | Koniku Inc. | Systems for detection |
| EP3606669A1 (en) | 2017-04-03 | 2020-02-12 | The Charles Stark Draper Laboratory, Inc. | Microfluidic system for evaluation of chemotherapeutic and immunotherapeutic drugs |
| CN111788471B (zh) | 2017-11-14 | 2023-12-12 | 思迪赛特诊断有限公司 | 用于光学测量的样品载体 |
| WO2020081288A1 (en) * | 2018-10-14 | 2020-04-23 | Arizona Board Of Regents On Behalf Of Arizona State University | Microfluidic devices and methods incorporating organized three-dimensional tissue constructs |
| US12305153B2 (en) * | 2019-05-13 | 2025-05-20 | Newsouth Innovations Pty Limited | Microfluidic device and method of use for cell culture |
| AU2020370212B2 (en) | 2019-10-22 | 2025-09-25 | S.D. Sight Diagnostics Ltd | Accounting for errors in optical measurements |
| CN114787625B (zh) | 2019-12-12 | 2026-01-13 | 思迪赛特诊断有限公司 | 检测血液样品中的血小板 |
| BR112022011312A2 (pt) | 2019-12-12 | 2022-08-23 | S D Sight Diagnostics Ltd | Análise de um analito disposto dentro de um meio |
| JP7677972B2 (ja) | 2019-12-12 | 2025-05-15 | エス．ディー．サイト ダイアグノスティックス リミテッド | カラー血液スミア画像の人工的な生成 |
| CN111068516B (zh) * | 2020-01-18 | 2023-11-14 | 浙江大学 | 多元强化耦合智能调控防止高粘性灰在催化剂表面沉积的系统及方法 |
| GB202002010D0 (en) * | 2020-02-13 | 2020-04-01 | Univ Of Nottingham Research | Microtopographies and uses thereof |
| US20240034973A1 (en) * | 2020-12-02 | 2024-02-01 | Ushio Denki Kabushiki Kaisha | Culture vessel, and method of using culture vessel |
| EP4281534A4 (en) * | 2021-01-22 | 2024-03-13 | Hewlett-Packard Development Company, L.P. | MICROFLUIDIC DEVICE CHAMBER PILLARS |
| US20240299942A1 (en) * | 2021-06-30 | 2024-09-12 | Hewlett-Packard Development Company, L.P. | Microfluidic device chamber pillars |
| CN114453039B (zh) * | 2022-02-08 | 2025-09-30 | 浙江扬清芯片技术有限公司 | 一种离心式微流控芯片点阵阀结构 |
| US12186748B2 (en) | 2022-06-24 | 2025-01-07 | Hewlett-Packard Development Company, L.P. | Self-priming microfluidic structures |
| CN115742293A (zh) * | 2022-11-01 | 2023-03-07 | 福建星海通信科技有限公司 | 一种基于dlp3d打印的生物应用微流体器件制备方法 |
| CN121446571A (zh) * | 2026-01-05 | 2026-02-03 | 四川迪亚生物科技集团有限公司 | 一种基于乏氧微环境的微流控芯片制备方法及其应用 |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1437404A (en) | 1973-09-24 | 1976-05-26 | Kaye S | Method and means for assaying biological factors demonstrating quantal response |
| DE3275216D1 (en) | 1981-09-18 | 1987-02-26 | Bbc Brown Boveri & Cie | Storage cavern for air under constant pressure with a hydraulic pressure compensation for a gas turbine power plant |
| DE4116727C2 (de) | 1991-05-17 | 1995-09-07 | Uwe Dr Marx | Verfahren und Vorrichtung zur gleichzeitigen Kultivierung unterschiedlicher Säugerzellen |
| DE4132379A1 (de) | 1991-09-28 | 1993-04-08 | Kernforschungsz Karlsruhe | Substrat fuer zellkulturen und kultur von zellen oder zellaggregaten |
| CA2175660C (en) | 1993-11-02 | 2005-07-05 | Charles S. Naiman | Control of cell growth |
| FR2741357B1 (fr) | 1995-11-22 | 1998-01-16 | Corning Inc | Procede de fabrication d'une plaquette de support d'un reseau bidimensionnel de micropuits, notamment pour essais ou cultures biologiques |
| US6391578B2 (en) | 1997-04-09 | 2002-05-21 | 3M Innovative Properties Company | Method and devices for partitioning biological sample liquids into microvolumes |
| CH692583A5 (de) | 1998-03-03 | 2002-08-15 | Weidmann H Ag | Kulturgefäss. |
| GB9805214D0 (en) | 1998-03-11 | 1998-05-06 | Univ Glasgow | Cell adhesion |
| WO1999047922A2 (en) | 1998-03-18 | 1999-09-23 | Massachusetts Institute Of Technology | Vascularized perfused microtissue/micro-organ arrays |
| JP2000231007A (ja) | 1999-02-09 | 2000-08-22 | Ricoh Opt Ind Co Ltd | 凹型微細形状のアレイ状パターン形成方法及びその形成方法を用いて製作される平板型レンズアレイ及び液晶表示素子及び平板型オイルトラップ |
| US7033821B2 (en) | 2000-11-08 | 2006-04-25 | Surface Logix, Inc. | Device for monitoring cell motility in real-time |
| US7211209B2 (en) | 2000-11-08 | 2007-05-01 | Surface Logix, Inc. | Method of making device for arraying biomolecules and for monitoring cell motility in real-time |
| US6653124B1 (en) | 2000-11-10 | 2003-11-25 | Cytoplex Biosciences Inc. | Array-based microenvironment for cell culturing, cell monitoring and drug-target validation |
| US7018418B2 (en) | 2001-01-25 | 2006-03-28 | Tecomet, Inc. | Textured surface having undercut micro recesses in a surface |
| US7288405B2 (en) | 2001-04-25 | 2007-10-30 | Cornell Research Foundation, Inc. | Devices and methods for pharmacokinetic-based cell culture system |
| ATE406436T1 (de) | 2001-07-26 | 2008-09-15 | Transparent Inc | Konstrukt aus kultivierten zellen mit spheroiden von kultivierten tierzellen und verwendung davon |
| US20030064000A1 (en) | 2001-09-24 | 2003-04-03 | Wilson Burgess | Methods of sterilizing biological mixtures using stabilizer mixtures |
| WO2003101618A1 (en) | 2002-05-31 | 2003-12-11 | Cancer Research Technology Ltd | Substrate for holding an array of experimental samples |
| US20040067585A1 (en) | 2002-10-07 | 2004-04-08 | Yu-Chi Wang | Cell cultivation surface and method of making the same |
| US7190449B2 (en) | 2002-10-28 | 2007-03-13 | Nanopoint, Inc. | Cell tray |
| EP2233564A3 (en) | 2002-10-30 | 2012-11-21 | Hitachi, Ltd. | Cell culture sheet comprising a functional substrate with a group of columnar micro-pillars and its manufacturing method |
| US7799378B2 (en) | 2002-11-27 | 2010-09-21 | Japan Science And Technology Agency | Process for producing micropillar structure |
| US7329537B2 (en) | 2003-04-17 | 2008-02-12 | Nexcelom Bioscience, Llc | Micro-pattern embedded plastic optical film device for cell-based assays |
| EP1636332A4 (en) | 2003-05-06 | 2007-08-29 | Bellbrook Labs Llc | THREE DIMENSIONAL CELL CULTURES IN A MICROMASSIFICATION FLUID PROCESSING SYSTEM |
| EP1626278A3 (en) | 2004-08-03 | 2006-06-21 | OnChip Cellomics Consortium | Cellomics system |
| WO2006052223A1 (en) | 2004-11-11 | 2006-05-18 | Agency For Science, Technology And Research | Cell culture device |
| US20060228386A1 (en) | 2005-02-22 | 2006-10-12 | University Of Tennessee Research Foundation | Polymeric microstructures |
| US20080227656A1 (en) | 2005-04-26 | 2008-09-18 | Flemming Besenbacher | Biosurface Structure Array |
| GB2427688A (en) | 2005-06-28 | 2007-01-03 | Inogen S A | Microwell plate |
| CA2621658C (en) | 2005-09-15 | 2012-10-23 | Sweet Power Inc. | Microbial fuel cell with flexible substrate and micro-pillar structure |
| US20070249044A1 (en) | 2006-01-30 | 2007-10-25 | University Of Illinois At Chicago | Microstructures in three dimensional gel suspensions for growth of cells |
| JP4506987B2 (ja) | 2006-07-21 | 2010-07-21 | 株式会社日立ハイテクノロジーズ | エネルギ線硬化型樹脂の転写方法、転写装置及びディスクまたは半導体デバイス |
| JP5013584B2 (ja) | 2006-08-30 | 2012-08-29 | 株式会社日立製作所 | 軟骨細胞の培養方法および軟骨細胞 |
-
2010
- 2010-03-03 US US12/716,680 patent/US8481303B2/en not_active Expired - Fee Related
- 2010-10-12 EP EP10766202A patent/EP2488628A2/en not_active Withdrawn
- 2010-10-12 JP JP2012534269A patent/JP2013507922A/ja not_active Abandoned
- 2010-10-12 WO PCT/US2010/052212 patent/WO2011046884A2/en not_active Ceased
- 2010-10-12 CN CN2010800468967A patent/CN102612554A/zh active Pending
-
2013
- 2013-06-06 US US13/911,490 patent/US8679843B2/en not_active Expired - Fee Related
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11744708B2 (en) | 2016-02-16 | 2023-09-05 | Koninklijke Philips N.V. | Surface topographies for altering the physiology of living cells |
| JP2019513409A (ja) * | 2016-02-16 | 2019-05-30 | マテリオミクス ベーフェー | 生細胞の生理機能を変化させるための表面トポグラフィ |
| JP7711738B2 (ja) | 2016-02-16 | 2025-07-23 | コーニンクレッカ フィリップス エヌ ヴェ | 生細胞の生理機能を変化させるための表面トポグラフィ |
| JP2024009986A (ja) * | 2016-02-16 | 2024-01-23 | コーニンクレッカ フィリップス エヌ ヴェ | 生細胞の生理機能を変化させるための表面トポグラフィ |
| JP7370370B2 (ja) | 2016-02-16 | 2023-10-27 | コーニンクレッカ フィリップス エヌ ヴェ | 生細胞の生理機能を変化させるための表面トポグラフィ |
| JP7026640B2 (ja) | 2016-02-16 | 2022-02-28 | コーニンクレッカ フィリップス エヌ ヴェ | 生細胞の生理機能を変化させるための表面トポグラフィ |
| JP2022068137A (ja) * | 2016-02-16 | 2022-05-09 | コーニンクレッカ フィリップス エヌ ヴェ | 生細胞の生理機能を変化させるための表面トポグラフィ |
| JP2019513416A (ja) * | 2016-03-29 | 2019-05-30 | エスエムエスバイオテック インコーポレイテッドＳｍｓｂｉｏｔｅｃｈ，Ｉｎｃ． | 小型運動性幹細胞を使用した組成物および方法 |
| JP7002530B2 (ja) | 2016-03-29 | 2022-02-04 | エスエムエスバイオテック インコーポレイテッド | 小型運動性幹細胞を使用した組成物および方法 |
| KR102579906B1 (ko) | 2017-03-31 | 2023-09-19 | 다나-파버 캔서 인스티튜트 인크. | ３d 미세유체 세포 배양 장치를 사용하여 종양 세포 구상체를 평가하는 방법 |
| JP7284095B2 (ja) | 2017-03-31 | 2023-05-30 | デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド | ３ｄマイクロ流体細胞培養デバイスを使用して腫瘍細胞スフェロイドを評価するための方法 |
| US11572590B2 (en) | 2017-03-31 | 2023-02-07 | Dana-Farber Cancer Institute, Inc. | Methods for evaluating tumor cell spheroids using 3D microfluidic cell culture device |
| JP2020515260A (ja) * | 2017-03-31 | 2020-05-28 | デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド | ３ｄマイクロ流体細胞培養デバイスを使用して腫瘍細胞スフェロイドを評価するための方法 |
| US12116636B2 (en) | 2017-03-31 | 2024-10-15 | Dana-Farber Cancer Institute, Inc. | Methods for evaluating tumor cell spheroids using 3D microfluidic cell culture device |
| KR20190130627A (ko) * | 2017-03-31 | 2019-11-22 | 다나-파버 캔서 인스티튜트 인크. | ３d 미세유체 세포 배양 장치를 사용하여 종양 세포 구상체를 평가하는 방법 |
| KR101979145B1 (ko) * | 2018-01-30 | 2019-05-15 | 서강대학교산학협력단 | 신경줄기세포 배양용 미세유체칩 및 이의 용도 |
| WO2020101242A1 (ko) * | 2018-11-13 | 2020-05-22 | 엠비디 주식회사 | 세포 배양 플레이트 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102612554A (zh) | 2012-07-25 |
| WO2011046884A3 (en) | 2012-01-26 |
| EP2488628A2 (en) | 2012-08-22 |
| US20110086427A1 (en) | 2011-04-14 |
| US8679843B2 (en) | 2014-03-25 |
| US20130273589A1 (en) | 2013-10-17 |
| WO2011046884A2 (en) | 2011-04-21 |
| US8481303B2 (en) | 2013-07-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2013507922A (ja) | 細胞培養用マイクロ流体デバイス | |
| Goral et al. | Perfusion-based microfluidic device for three-dimensional dynamic primary human hepatocyte cell culture in the absence of biological or synthetic matrices or coagulants | |
| Chandra | Programmable cell adhesion encoded by DNA hybridization | |
| Torisawa et al. | A multicellular spheroid array to realize spheroid formation, culture, and viability assay on a chip | |
| Choe et al. | Microfluidic Gut-liver chip for reproducing the first pass metabolism | |
| US9115340B2 (en) | Microfluidic continuous flow device | |
| Gupta et al. | Microfluidics‐based 3D cell culture models: Utility in novel drug discovery and delivery research | |
| Sodunke et al. | Micropatterns of Matrigel for three-dimensional epithelial cultures | |
| Sivagnanam et al. | Exploring living multicellular organisms, organs, and tissues using microfluidic systems | |
| EP2721141B1 (en) | Device and method for culturing cells in a biomimetic environment | |
| US9121847B2 (en) | Three-dimensional microfluidic platforms and methods of use thereof | |
| Ahmed et al. | Human liver microtissue spheroids in hollow fiber membrane bioreactor | |
| US12163112B2 (en) | Production of cellular spheroids | |
| JP2007501633A (ja) | 微小規模の流体ハンドリングシステムにおける三次元細胞培養 | |
| Shih et al. | A microfluidic device mimicking acinar concentration gradients across the liver acinus | |
| Lee et al. | Fabrication of a self-assembled and vascularized tumor array via bioprinting on a microfluidic chip | |
| Kojima et al. | Fabrication of microchannel networks in multicellular spheroids | |
| Han et al. | Hydrophobic patterning‐based 3D microfluidic cell culture assay | |
| Gong et al. | Large-scale patterning of single cells and cell clusters in hydrogels | |
| CA3043708A1 (en) | Chip platforms for microarray 3d bioprinting | |
| Zhu et al. | Microfluidic-based platforms for cell-to-cell communication studies | |
| Yao et al. | Biological gel-based microchamber array for tumor cell proliferation and migration studies in well-controlled biochemical gradients | |
| Zheng et al. | Precise manipulation of cell behaviors on surfaces for construction of tissue/organs | |
| Torabi et al. | Cassie–Baxter surfaces for reversible, barrier-free integration of microfluidics and 3D cell culture | |
| Lee et al. | Constrained spheroids/organoids in perfusion culture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20130402 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20130402 |
|
| A762 | Written abandonment of application |
Free format text: JAPANESE INTERMEDIATE CODE: A762 Effective date: 20131007 |