JP2012506710A - 宿主細胞中での糖鎖付加タンパク質の産生のための新規ツール - Google Patents

宿主細胞中での糖鎖付加タンパク質の産生のための新規ツール Download PDF

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JP2012506710A
JP2012506710A JP2011533616A JP2011533616A JP2012506710A JP 2012506710 A JP2012506710 A JP 2012506710A JP 2011533616 A JP2011533616 A JP 2011533616A JP 2011533616 A JP2011533616 A JP 2011533616A JP 2012506710 A JP2012506710 A JP 2012506710A
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cell
activity
glycoprotein
type
seq
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JP2012506710A5 (enExample
Inventor
ヘレニウス，ヨンネ
ノイペルト，クリスティーネ
エビ，マルクス
ナサブ，ファルノウシュ パルサイエ
フライ，アレクサンダー，ダニエル
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Lonza AG
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Lonza AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99018—Dolichyl-diphosphooligosaccharide—protein glycotransferase (2.4.99.18)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
JP2011533616A 2008-10-31 2009-10-31 宿主細胞中での糖鎖付加タンパク質の産生のための新規ツール Withdrawn JP2012506710A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US19802308P 2008-10-31 2008-10-31
EP08400044 2008-10-31
EP08400044.7 2008-10-31
US61/198,023 2008-10-31
PCT/EP2009/007816 WO2010049177A1 (en) 2008-10-31 2009-10-31 Novel tools for the production of glycosylated proteins in host cells

Publications (2)

Publication Number Publication Date
JP2012506710A true JP2012506710A (ja) 2012-03-22
JP2012506710A5 JP2012506710A5 (enExample) 2012-11-08

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JP2011533616A Withdrawn JP2012506710A (ja) 2008-10-31 2009-10-31 宿主細胞中での糖鎖付加タンパク質の産生のための新規ツール

Country Status (9)

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US (1) US20110207214A1 (enExample)
EP (1) EP2350123A1 (enExample)
JP (1) JP2012506710A (enExample)
CN (1) CN102203123A (enExample)
AU (1) AU2009309925A1 (enExample)
CA (1) CA2741964A1 (enExample)
IL (1) IL212580A0 (enExample)
TW (1) TW201028431A (enExample)
WO (1) WO2010049177A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017505620A (ja) * 2014-01-29 2017-02-23 アムジエン・インコーポレーテツド 組換えタンパク質のグリコシル化を調節するためのｎ−グリコシル化経路制御因子の過剰発現
JP2018061520A (ja) * 2018-01-26 2018-04-19 東洋紡株式会社 アスペルギルス属微生物が生産するタンパク質のｎ型糖鎖構造を改変する方法

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100787073B1 (ko) * 2000-06-28 2007-12-21 글리코파이, 인크. 변형된 당단백질의 제조방법
CN102858949B (zh) 2010-02-24 2016-07-06 默沙东公司 用于增加在巴氏毕赤酵母中生产的治疗性糖蛋白上的n-糖基化位点占据的方法
RU2013128619A (ru) * 2010-11-24 2014-12-27 Новартис Интернэшнл Фармасьютикал Лтд. Слитные ферменты
WO2013123034A1 (en) * 2012-02-16 2013-08-22 Merck Sharp & Dohme Corp. Methods for reducing mannosyltransferase activity in yeast
CN102586316B (zh) * 2012-02-16 2013-08-07 中国科学院微生物研究所 人源糖基化改造的汉逊酵母
CN102839157A (zh) * 2012-07-12 2012-12-26 扬州大学 稳定共表达hST3GalIV和β1-4GalT1的MDCK细胞系及构建方法和应用
JP6356229B2 (ja) * 2013-06-04 2018-07-11 アイコン ジェネティクス ゲーエムベーハー 植物産生糖タンパク質及び組換え糖タンパク質のｎ−グリコシル化部位占有率を調節する方法
PL3019621T3 (pl) * 2013-07-10 2019-05-31 Glykos Finland Oy Wytwarzanie glikoprotein o zwiększonym zajmowaniu miejsca N-glikozylacji
CA2954974A1 (en) 2014-07-21 2016-01-28 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
CN105372214B (zh) * 2014-08-18 2019-06-28 中国科学院上海有机化学研究所 一种鉴定新红细胞生成刺激蛋白的n-连接寡糖结构的方法
CN104784754B (zh) * 2015-03-04 2017-05-10 重庆市畜牧科学院 一种蚕丝人工血管及制备方法
CN106867966B (zh) * 2015-12-11 2020-11-24 复旦大学 稳定表达核心α(1,3)岩藻糖基转移酶的脊椎动物细胞系及其制备方法
CN108179160B (zh) * 2018-01-29 2021-05-11 江南大学 一种植烷醇连接的高甘露糖型寡糖的制备方法
EP3788164A4 (en) * 2018-05-01 2022-01-26 Conagen Inc. Recombinant organisms and methods for producing glycomolecules with high glycan occupancy
CN110687035A (zh) * 2019-10-10 2020-01-14 沈阳万类生物科技有限公司 Annexin V-Light 650凋亡检测试剂盒
CN117377752A (zh) * 2021-03-11 2024-01-09 二进国际(美国)有限公司 用于生产具有减少的或不含n-连接的糖基化的外源蛋白的遗传修饰的丝状真菌

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7507573B2 (en) * 2003-11-14 2009-03-24 Vib, Vzw Modification of protein glycosylation in methylotrophic yeast
CA2573745A1 (en) * 2004-07-21 2007-01-12 Glycofi, Inc. Immunoglobulins comprising predominantly a glcnac2man3glcnac2 glycoform

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017505620A (ja) * 2014-01-29 2017-02-23 アムジエン・インコーポレーテツド 組換えタンパク質のグリコシル化を調節するためのｎ−グリコシル化経路制御因子の過剰発現
JP2019088305A (ja) * 2014-01-29 2019-06-13 アムジエン・インコーポレーテツド 組換えタンパク質のグリコシル化を調節するためのｎ−グリコシル化経路制御因子の過剰発現
JP2021040638A (ja) * 2014-01-29 2021-03-18 アムジエン・インコーポレーテツド 組換えタンパク質のグリコシル化を調節するためのｎ−グリコシル化経路制御因子の過剰発現
JP7034236B2 (ja) 2014-01-29 2022-03-11 アムジエン・インコーポレーテツド 組換えタンパク質のグリコシル化を調節するためのｎ－グリコシル化経路制御因子の過剰発現
KR20220086703A (ko) * 2014-01-29 2022-06-23 암젠 인크 재조합 단백질의 글리코실화를 조절하기 위한 n-글리코실화 경로 조절자의 과발현
KR102519540B1 (ko) 2014-01-29 2023-04-10 암젠 인크 재조합 단백질의 글리코실화를 조절하기 위한 n-글리코실화 경로 조절자의 과발현
JP2018061520A (ja) * 2018-01-26 2018-04-19 東洋紡株式会社 アスペルギルス属微生物が生産するタンパク質のｎ型糖鎖構造を改変する方法

Also Published As

Publication number Publication date
EP2350123A1 (en) 2011-08-03
CN102203123A (zh) 2011-09-28
WO2010049177A1 (en) 2010-05-06
US20110207214A1 (en) 2011-08-25
TW201028431A (en) 2010-08-01
AU2009309925A1 (en) 2010-05-06
IL212580A0 (en) 2011-07-31
CA2741964A1 (en) 2010-05-06

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