JP2011530296A5 - - Google Patents
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- Publication number
- JP2011530296A5 JP2011530296A5 JP2011522302A JP2011522302A JP2011530296A5 JP 2011530296 A5 JP2011530296 A5 JP 2011530296A5 JP 2011522302 A JP2011522302 A JP 2011522302A JP 2011522302 A JP2011522302 A JP 2011522302A JP 2011530296 A5 JP2011530296 A5 JP 2011530296A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- temperature
- target nucleic
- labeled nucleic
- label
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims 23
- 108020004707 nucleic acids Proteins 0.000 claims 15
- 150000007523 nucleic acids Chemical class 0.000 claims 15
- 102000039446 nucleic acids Human genes 0.000 claims 15
- 108020004711 Nucleic Acid Probes Proteins 0.000 claims 14
- 239000002853 nucleic acid probe Substances 0.000 claims 14
- 239000007850 fluorescent dye Substances 0.000 claims 6
- 239000000523 sample Substances 0.000 claims 5
- 238000009396 hybridization Methods 0.000 claims 3
- 230000001419 dependent effect Effects 0.000 claims 2
- 238000005259 measurement Methods 0.000 claims 2
- 239000011541 reaction mixture Substances 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 238000000137 annealing Methods 0.000 claims 1
- 239000006172 buffering agent Substances 0.000 claims 1
- 238000004925 denaturation Methods 0.000 claims 1
- 230000036425 denaturation Effects 0.000 claims 1
- 238000004020 luminiscence type Methods 0.000 claims 1
- 230000003252 repetitive effect Effects 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13604008P | 2008-08-08 | 2008-08-08 | |
| US61/136,040 | 2008-08-08 | ||
| PCT/US2009/053253 WO2010017543A1 (en) | 2008-08-08 | 2009-08-10 | Detection algorithm for pcr assay |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2011530296A JP2011530296A (ja) | 2011-12-22 |
| JP2011530296A5 true JP2011530296A5 (https=) | 2012-02-09 |
Family
ID=41259286
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011522302A Pending JP2011530296A (ja) | 2008-08-08 | 2009-08-10 | Ｐｃｒアッセイのための検出アルゴリズム |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20110151461A1 (https=) |
| EP (1) | EP2315844A1 (https=) |
| JP (1) | JP2011530296A (https=) |
| KR (1) | KR20110052681A (https=) |
| CN (1) | CN102112632A (https=) |
| AU (1) | AU2009279457A1 (https=) |
| BR (1) | BRPI0917434A2 (https=) |
| CA (1) | CA2733186A1 (https=) |
| RU (1) | RU2011107993A (https=) |
| WO (1) | WO2010017543A1 (https=) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102301007A (zh) * | 2008-12-10 | 2011-12-28 | 史密斯探测公司 | 使用随温度而变的杂交对核酸序列进行鉴定与区分 |
| US20130203057A1 (en) * | 2012-01-20 | 2013-08-08 | Biohelix Corporation | Step-wise detection of multiple target sequences in isothermal nucleic acid amplification reactions |
| CN114717296B (zh) | 2012-02-03 | 2025-04-04 | 加州理工学院 | 多路生化测定中信号的编码和解码 |
| EP3901243A1 (en) * | 2012-08-03 | 2021-10-27 | California Institute of Technology | Multiplexing and quantification in pcr with reduced hardware and requirements |
| WO2015147370A1 (en) * | 2014-03-28 | 2015-10-01 | Seegene, Inc. | Detection of target nucleic acid sequences using different detection temperatures |
| WO2016093620A1 (en) | 2014-12-09 | 2016-06-16 | Seegene, Inc. | Differentiation of signals for target nucleic acid sequences |
| US20170362646A1 (en) * | 2014-12-09 | 2017-12-21 | Seegene, Inc. | Detection of target nucleic acid sequences using different detection temperatures and reference values |
| KR102110999B1 (ko) * | 2016-01-26 | 2020-05-14 | 주식회사 씨젠 | 타겟 핵산 서열에 대한 시그널을 제공하는 방법 |
| JP2021511056A (ja) * | 2018-01-22 | 2021-05-06 | ルミネックス コーポレーション | 不連続融解分析のための方法および組成物 |
| US12454720B2 (en) | 2018-04-17 | 2025-10-28 | ChromaCode, Inc. | Methods and systems for multiplex analysis |
| US12203129B2 (en) | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| US20240418649A1 (en) * | 2021-10-21 | 2024-12-19 | Seegene, Inc. | Device and method for reading positive/negative with respect to target assay substance in sample |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5475098A (en) * | 1994-06-14 | 1995-12-12 | The United States Of America As Represented By The Department Of Health And Human Services | Distinctive DNA sequence of E. coli 0157:H7 and its use for the rapid, sensitive and specific detection of 0157:H7 and other enterohemorrhagic E. coli |
| AU762728B2 (en) * | 1998-07-02 | 2003-07-03 | Gen-Probe Incorporated | Molecular torches |
| US8900811B2 (en) * | 2000-11-16 | 2014-12-02 | Caliper Life Sciences, Inc. | Method and apparatus for generating thermal melting curves in a microfluidic device |
| US20050202470A1 (en) * | 2000-11-16 | 2005-09-15 | Caliper Life Sciences, Inc. | Binding assays using molecular melt curves |
| US20040005720A1 (en) * | 2001-10-30 | 2004-01-08 | Cremer Paul S. | Method and apparatus for temperature gradient microfluidics |
| US7831417B2 (en) * | 2005-11-14 | 2010-11-09 | Gen-Probe Incorporated | Parametric calibration method |
-
2009
- 2009-08-10 CN CN2009801308017A patent/CN102112632A/zh active Pending
- 2009-08-10 AU AU2009279457A patent/AU2009279457A1/en not_active Abandoned
- 2009-08-10 BR BRPI0917434A patent/BRPI0917434A2/pt not_active Application Discontinuation
- 2009-08-10 JP JP2011522302A patent/JP2011530296A/ja active Pending
- 2009-08-10 WO PCT/US2009/053253 patent/WO2010017543A1/en not_active Ceased
- 2009-08-10 KR KR1020117005036A patent/KR20110052681A/ko not_active Withdrawn
- 2009-08-10 EP EP09791325A patent/EP2315844A1/en not_active Withdrawn
- 2009-08-10 RU RU2011107993/10A patent/RU2011107993A/ru not_active Application Discontinuation
- 2009-08-10 US US13/057,735 patent/US20110151461A1/en not_active Abandoned
- 2009-08-10 CA CA2733186A patent/CA2733186A1/en not_active Abandoned
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