CN102112632A - 用于pcr分析的检测算法 - Google Patents

用于pcr分析的检测算法 Download PDF

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Publication number
CN102112632A
CN102112632A CN2009801308017A CN200980130801A CN102112632A CN 102112632 A CN102112632 A CN 102112632A CN 2009801308017 A CN2009801308017 A CN 2009801308017A CN 200980130801 A CN200980130801 A CN 200980130801A CN 102112632 A CN102112632 A CN 102112632A
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China
Prior art keywords
nucleic acid
target nucleic
temperature
probe
slope
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Pending
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CN2009801308017A
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English (en)
Chinese (zh)
Inventor
约翰·罗伯特·林克
巴里·爱德华·博伊斯
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Smiths Detection Inc
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Smiths Detection Inc
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Application filed by Smiths Detection Inc filed Critical Smiths Detection Inc
Publication of CN102112632A publication Critical patent/CN102112632A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CN2009801308017A 2008-08-08 2009-08-10 用于pcr分析的检测算法 Pending CN102112632A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13604008P 2008-08-08 2008-08-08
US61/136,040 2008-08-08
PCT/US2009/053253 WO2010017543A1 (en) 2008-08-08 2009-08-10 Detection algorithm for pcr assay

Publications (1)

Publication Number Publication Date
CN102112632A true CN102112632A (zh) 2011-06-29

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ID=41259286

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2009801308017A Pending CN102112632A (zh) 2008-08-08 2009-08-10 用于pcr分析的检测算法

Country Status (10)

Country Link
US (1) US20110151461A1 (https=)
EP (1) EP2315844A1 (https=)
JP (1) JP2011530296A (https=)
KR (1) KR20110052681A (https=)
CN (1) CN102112632A (https=)
AU (1) AU2009279457A1 (https=)
BR (1) BRPI0917434A2 (https=)
CA (1) CA2733186A1 (https=)
RU (1) RU2011107993A (https=)
WO (1) WO2010017543A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106103740A (zh) * 2014-03-28 2016-11-09 Seegene株式会社 利用不同检测温度的靶核酸序列检测
CN111629831A (zh) * 2018-01-22 2020-09-04 卢米耐克斯公司 用于离散解链分析的方法和组合物

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012511328A (ja) * 2008-12-10 2012-05-24 スミスズ ディテクション インコーポレイティド 温度依存ハイブリダイゼーションを使用した核酸配列の同定及び区別
US20130203057A1 (en) * 2012-01-20 2013-08-08 Biohelix Corporation Step-wise detection of multiple target sequences in isothermal nucleic acid amplification reactions
EP4361609A3 (en) 2012-02-03 2024-07-17 California Institute of Technology Signal encoding and decoding in multiplexed biochemical assays
CN104662172B (zh) * 2012-08-03 2018-07-06 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
KR102084405B1 (ko) 2014-12-09 2020-03-04 주식회사 씨젠 타겟 핵산 서열에 대한 시그널의 구별
WO2016093619A1 (en) * 2014-12-09 2016-06-16 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures and reference values
KR102110999B1 (ko) * 2016-01-26 2020-05-14 주식회사 씨젠 타겟 핵산 서열에 대한 시그널을 제공하는 방법
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
EP4421814A4 (en) * 2021-10-21 2025-11-12 Seegene Inc APPROVAL AND METHOD FOR READING A POSITIVE/NEGATIVE RESULT WITH RESPECT TO A TARGET TEST SUBSTANCE IN A SAMPLE

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050202470A1 (en) * 2000-11-16 2005-09-15 Caliper Life Sciences, Inc. Binding assays using molecular melt curves
US20070026421A1 (en) * 2000-11-16 2007-02-01 Caliper Life Sciences, Inc. Method and apparatus for generating thermal melting curves in a microfluidic device

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5475098A (en) * 1994-06-14 1995-12-12 The United States Of America As Represented By The Department Of Health And Human Services Distinctive DNA sequence of E. coli 0157:H7 and its use for the rapid, sensitive and specific detection of 0157:H7 and other enterohemorrhagic E. coli
ES2330812T3 (es) * 1998-07-02 2009-12-15 Gen-Probe Incorporated Antorchas moleculares.
US20040005720A1 (en) * 2001-10-30 2004-01-08 Cremer Paul S. Method and apparatus for temperature gradient microfluidics
CA2627259C (en) * 2005-11-14 2016-02-23 Gen-Probe Incorporated Parametric calibration method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050202470A1 (en) * 2000-11-16 2005-09-15 Caliper Life Sciences, Inc. Binding assays using molecular melt curves
US20070026421A1 (en) * 2000-11-16 2007-02-01 Caliper Life Sciences, Inc. Method and apparatus for generating thermal melting curves in a microfluidic device

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
TSOURKAS A. ET AL,,: ""Hybridization kinetics and thermodynamics of molecular beacons"", 《NUCLEIC ACIDS RESEARCH》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106103740A (zh) * 2014-03-28 2016-11-09 Seegene株式会社 利用不同检测温度的靶核酸序列检测
CN110452962A (zh) * 2014-03-28 2019-11-15 Seegene株式会社 利用不同检测温度的靶核酸序列检测
CN111893171A (zh) * 2014-03-28 2020-11-06 Seegene株式会社 利用不同检测温度的靶核酸序列检测
CN111629831A (zh) * 2018-01-22 2020-09-04 卢米耐克斯公司 用于离散解链分析的方法和组合物
US11390902B2 (en) 2018-01-22 2022-07-19 Luminex Corporation Methods and compositions for discrete melt analysis

Also Published As

Publication number Publication date
BRPI0917434A2 (pt) 2015-12-01
EP2315844A1 (en) 2011-05-04
KR20110052681A (ko) 2011-05-18
WO2010017543A1 (en) 2010-02-11
RU2011107993A (ru) 2012-09-20
AU2009279457A1 (en) 2010-02-11
US20110151461A1 (en) 2011-06-23
JP2011530296A (ja) 2011-12-22
CA2733186A1 (en) 2010-02-11

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Application publication date: 20110629