JP2011527192A5 - - Google Patents
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- Publication number
- JP2011527192A5 JP2011527192A5 JP2011517241A JP2011517241A JP2011527192A5 JP 2011527192 A5 JP2011527192 A5 JP 2011527192A5 JP 2011517241 A JP2011517241 A JP 2011517241A JP 2011517241 A JP2011517241 A JP 2011517241A JP 2011527192 A5 JP2011527192 A5 JP 2011527192A5
- Authority
- JP
- Japan
- Prior art keywords
- cells
- cell
- fixative
- storage
- reference material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cell Anatomy 0.000 claims 71
- 238000000034 method Methods 0.000 claims 29
- 239000000834 fixative Substances 0.000 claims 10
- PHEDXBVPIONUQT-UHFFFAOYSA-N Cocarcinogen A1 Natural products CCCCCCCCCCCCCC(=O)OC1C(C)C2(O)C3C=C(C)C(=O)C3(O)CC(CO)=CC2C2C1(OC(C)=O)C2(C)C PHEDXBVPIONUQT-UHFFFAOYSA-N 0.000 claims 8
- 239000003226 mitogen Substances 0.000 claims 8
- PHEDXBVPIONUQT-RGYGYFBISA-N phorbol 13-acetate 12-myristate Chemical compound C([C@]1(O)C(=O)C(C)=C[C@H]1[C@@]1(O)[C@H](C)[C@H]2OC(=O)CCCCCCCCCCCCC)C(CO)=C[C@H]1[C@H]1[C@]2(OC(C)=O)C1(C)C PHEDXBVPIONUQT-RGYGYFBISA-N 0.000 claims 8
- 238000004519 manufacturing process Methods 0.000 claims 7
- 239000012925 reference material Substances 0.000 claims 7
- 239000000203 mixture Substances 0.000 claims 6
- 102000004127 Cytokines Human genes 0.000 claims 5
- 108090000695 Cytokines Proteins 0.000 claims 5
- 238000004108 freeze drying Methods 0.000 claims 5
- 241001465754 Metazoa Species 0.000 claims 4
- 229930040373 Paraformaldehyde Natural products 0.000 claims 4
- PGHMRUGBZOYCAA-ADZNBVRBSA-N ionomycin Chemical compound O1[C@H](C[C@H](O)[C@H](C)[C@H](O)[C@H](C)/C=C/C[C@@H](C)C[C@@H](C)C(/O)=C/C(=O)[C@@H](C)C[C@@H](C)C[C@@H](CCC(O)=O)C)CC[C@@]1(C)[C@@H]1O[C@](C)([C@@H](C)O)CC1 PGHMRUGBZOYCAA-ADZNBVRBSA-N 0.000 claims 4
- PGHMRUGBZOYCAA-UHFFFAOYSA-N ionomycin Natural products O1C(CC(O)C(C)C(O)C(C)C=CCC(C)CC(C)C(O)=CC(=O)C(C)CC(C)CC(CCC(O)=O)C)CCC1(C)C1OC(C)(C(C)O)CC1 PGHMRUGBZOYCAA-UHFFFAOYSA-N 0.000 claims 4
- 229920002866 paraformaldehyde Polymers 0.000 claims 4
- 230000004936 stimulating effect Effects 0.000 claims 4
- 239000003112 inhibitor Substances 0.000 claims 3
- 238000002156 mixing Methods 0.000 claims 3
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 claims 3
- 230000028327 secretion Effects 0.000 claims 3
- 229910021555 Chromium Chloride Inorganic materials 0.000 claims 2
- QSWDMMVNRMROPK-UHFFFAOYSA-K chromium(3+) trichloride Chemical compound [Cl-].[Cl-].[Cl-].[Cr+3] QSWDMMVNRMROPK-UHFFFAOYSA-K 0.000 claims 2
- 239000002577 cryoprotective agent Substances 0.000 claims 2
- 230000001419 dependent effect Effects 0.000 claims 2
- 239000007850 fluorescent dye Substances 0.000 claims 2
- 238000002955 isolation Methods 0.000 claims 2
- 238000002372 labelling Methods 0.000 claims 2
- 230000002062 proliferating effect Effects 0.000 claims 2
- 241000124008 Mammalia Species 0.000 claims 1
- KQNZDYYTLMIZCT-KQPMLPITSA-N brefeldin A Chemical compound O[C@@H]1\C=C\C(=O)O[C@@H](C)CCC\C=C\[C@@H]2C[C@H](O)C[C@H]21 KQNZDYYTLMIZCT-KQPMLPITSA-N 0.000 claims 1
- JUMGSHROWPPKFX-UHFFFAOYSA-N brefeldin-A Natural products CC1CCCC=CC2(C)CC(O)CC2(C)C(O)C=CC(=O)O1 JUMGSHROWPPKFX-UHFFFAOYSA-N 0.000 claims 1
- 230000010261 cell growth Effects 0.000 claims 1
- 238000007710 freezing Methods 0.000 claims 1
- 230000008014 freezing Effects 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 230000035755 proliferation Effects 0.000 claims 1
- 230000000638 stimulation Effects 0.000 claims 1
- -1 succinimidyl ester Chemical class 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0812757A GB0812757D0 (en) | 2008-07-11 | 2008-07-11 | Cell profliferation standards |
| GB0812755A GB0812755D0 (en) | 2008-07-11 | 2008-07-11 | Stimulated cell standards |
| GB0812757.3 | 2008-07-11 | ||
| GB0812755.7 | 2008-07-11 | ||
| PCT/GB2009/050815 WO2010004336A2 (en) | 2008-07-11 | 2009-07-09 | Stimulated cell standards |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2011527192A JP2011527192A (ja) | 2011-10-27 |
| JP2011527192A5 true JP2011527192A5 (https=) | 2012-07-05 |
| JP5816086B2 JP5816086B2 (ja) | 2015-11-17 |
Family
ID=41120025
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011517241A Expired - Fee Related JP5816086B2 (ja) | 2008-07-11 | 2009-07-09 | 刺激細胞標準 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9267956B2 (https=) |
| EP (1) | EP2316036B1 (https=) |
| JP (1) | JP5816086B2 (https=) |
| CN (1) | CN102144165B (https=) |
| AU (1) | AU2009269765B2 (https=) |
| BR (1) | BRPI0915903A8 (https=) |
| CA (1) | CA2730191C (https=) |
| ES (1) | ES2454320T3 (https=) |
| PT (1) | PT2316036E (https=) |
| WO (1) | WO2010004336A2 (https=) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2834619A1 (en) * | 2012-04-05 | 2015-02-11 | Renishaw Diagnostics Limited | A method for calibrating spectroscopy apparatus and equipment for use in the method |
| CN107576790A (zh) * | 2017-11-03 | 2018-01-12 | 深圳市巴德生物科技有限公司 | 一种冻干人淋巴细胞cd4表面抗原质控品及其制备方法 |
| CN107576788A (zh) * | 2017-11-03 | 2018-01-12 | 深圳市巴德生物科技有限公司 | 一种检测白细胞分化抗原的参照品及其制备方法 |
| CN109925323A (zh) * | 2019-03-29 | 2019-06-25 | 广东先康达生物科技有限公司 | 有效改善骨髓造血微环境的免疫细胞制剂 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IE76319B1 (en) * | 1990-07-23 | 1997-10-22 | Becton Dickinson Co | Preservation of cells as controls or standards in cellular analysis |
| AU700699B2 (en) * | 1993-07-05 | 1999-01-14 | Sheffield Teaching Hospitals National Health Service Trust, The | Preparation and stabilisation of cells |
| EP1428008B1 (en) * | 2001-09-06 | 2012-01-18 | Core Dynamics Limited | Improved method for freezing viable cells |
| US7354773B2 (en) * | 2003-05-14 | 2008-04-08 | Beckman Coulter, Inc. | Method and apparatus for preparing cell samples for intracellular antigen detection using flow cytometry |
| WO2006090096A1 (en) * | 2005-02-28 | 2006-08-31 | Sheffield Teaching Hospitals Nhs Foundation Trust | Composition, kit and method for fixing cells |
| JP2009526991A (ja) * | 2006-02-17 | 2009-07-23 | パオロ・ラ・コッラ | 増殖性疾患及び／又はコンホメーション病の診断方法 |
-
2009
- 2009-07-09 EP EP09785294.1A patent/EP2316036B1/en not_active Not-in-force
- 2009-07-09 CN CN200980134557.1A patent/CN102144165B/zh not_active Expired - Fee Related
- 2009-07-09 AU AU2009269765A patent/AU2009269765B2/en not_active Ceased
- 2009-07-09 CA CA2730191A patent/CA2730191C/en not_active Expired - Fee Related
- 2009-07-09 JP JP2011517241A patent/JP5816086B2/ja not_active Expired - Fee Related
- 2009-07-09 PT PT97852941T patent/PT2316036E/pt unknown
- 2009-07-09 US US13/003,661 patent/US9267956B2/en not_active Expired - Fee Related
- 2009-07-09 ES ES09785294.1T patent/ES2454320T3/es active Active
- 2009-07-09 WO PCT/GB2009/050815 patent/WO2010004336A2/en not_active Ceased
- 2009-07-09 BR BRPI0915903A patent/BRPI0915903A8/pt not_active IP Right Cessation
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