JP2011504094A5 - - Google Patents
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- JP2011504094A5 JP2011504094A5 JP2010531389A JP2010531389A JP2011504094A5 JP 2011504094 A5 JP2011504094 A5 JP 2011504094A5 JP 2010531389 A JP2010531389 A JP 2010531389A JP 2010531389 A JP2010531389 A JP 2010531389A JP 2011504094 A5 JP2011504094 A5 JP 2011504094A5
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Families Citing this family (105)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7968287B2 (en) | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
| WO2007081385A2 (en) | 2006-01-11 | 2007-07-19 | Raindance Technologies, Inc. | Microfluidic devices and methods of use in the formation and control of nanoreactors |
| EP2530167A1 (en) | 2006-05-11 | 2012-12-05 | Raindance Technologies, Inc. | Microfluidic Devices |
| US9562837B2 (en) | 2006-05-11 | 2017-02-07 | Raindance Technologies, Inc. | Systems for handling microfludic droplets |
| WO2008097559A2 (en) | 2007-02-06 | 2008-08-14 | Brandeis University | Manipulation of fluids and reactions in microfluidic systems |
| US8592221B2 (en) | 2007-04-19 | 2013-11-26 | Brandeis University | Manipulation of fluids, fluid components and reactions in microfluidic systems |
| US9211537B2 (en) | 2007-11-07 | 2015-12-15 | The University Of British Columbia | Microfluidic device and method of using same |
| EP4047367A1 (en) | 2008-07-18 | 2022-08-24 | Bio-Rad Laboratories, Inc. | Method for detecting target analytes with droplet libraries |
| US12038438B2 (en) | 2008-07-18 | 2024-07-16 | Bio-Rad Laboratories, Inc. | Enzyme quantification |
| US9180453B2 (en) * | 2008-08-15 | 2015-11-10 | University Of Washington | Method and apparatus for the discretization and manipulation of sample volumes |
| DE102008042054A1 (de) * | 2008-09-12 | 2010-03-18 | Robert Bosch Gmbh | Mikroventil, Mikropumpe sowie Herstellungsverfahren |
| WO2010111231A1 (en) | 2009-03-23 | 2010-09-30 | Raindance Technologies, Inc. | Manipulation of microfluidic droplets |
| JP2011062119A (ja) | 2009-09-16 | 2011-03-31 | Seiko Epson Corp | 生体試料定量用チップ |
| JP5441035B2 (ja) * | 2009-11-06 | 2014-03-12 | 国立大学法人北陸先端科学技術大学院大学 | 試料解析装置 |
| US20130022973A1 (en) * | 2010-01-15 | 2013-01-24 | Hansen Carl L G | Multiplex Amplification for the Detection of Nucleic Acid Variations |
| US10351905B2 (en) | 2010-02-12 | 2019-07-16 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
| US9399797B2 (en) | 2010-02-12 | 2016-07-26 | Raindance Technologies, Inc. | Digital analyte analysis |
| EP4484577A3 (en) | 2010-02-12 | 2025-03-26 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
| US9366632B2 (en) | 2010-02-12 | 2016-06-14 | Raindance Technologies, Inc. | Digital analyte analysis |
| JP5505646B2 (ja) * | 2010-09-06 | 2014-05-28 | セイコーエプソン株式会社 | 生体試料定量方法 |
| US9562897B2 (en) | 2010-09-30 | 2017-02-07 | Raindance Technologies, Inc. | Sandwich assays in droplets |
| EP3412778A1 (en) | 2011-02-11 | 2018-12-12 | Raindance Technologies, Inc. | Methods for forming mixed droplets |
| WO2012112804A1 (en) | 2011-02-18 | 2012-08-23 | Raindance Technoligies, Inc. | Compositions and methods for molecular labeling |
| EP2689029A1 (en) * | 2011-03-22 | 2014-01-29 | Life Technologies Corporation | Identification of linkage using multiplex digital pcr |
| EP3216872B1 (en) | 2011-06-02 | 2020-04-01 | Bio-Rad Laboratories, Inc. | Enzyme quantification |
| US20130178378A1 (en) * | 2011-06-09 | 2013-07-11 | Andrew C. Hatch | Multiplex digital pcr |
| US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
| CN103998394B (zh) | 2011-08-01 | 2016-08-17 | 德诺弗科学公司 | 细胞捕获系统和使用方法 |
| US9404864B2 (en) | 2013-03-13 | 2016-08-02 | Denovo Sciences, Inc. | System for imaging captured cells |
| US10466160B2 (en) | 2011-08-01 | 2019-11-05 | Celsee Diagnostics, Inc. | System and method for retrieving and analyzing particles |
| CN104350374B (zh) * | 2012-02-29 | 2018-04-27 | 富鲁达公司 | 用于使用微流体的多个单细胞捕获和处理的方法、系统和设备 |
| US9067189B2 (en) | 2012-03-30 | 2015-06-30 | General Electric Company | Microfluidic device and a related method thereof |
| EP2844768B1 (en) * | 2012-04-30 | 2019-03-13 | Raindance Technologies, Inc. | Digital analyte analysis |
| US20130316538A1 (en) * | 2012-05-23 | 2013-11-28 | International Business Machines Corporation | Surface morphology generation and transfer by spalling |
| US9821312B2 (en) | 2012-09-12 | 2017-11-21 | Bio-Rad Laboratories, Inc. | Integrated microfluidic system, method and kit for performing assays |
| US9752181B2 (en) | 2013-01-26 | 2017-09-05 | Denovo Sciences, Inc. | System and method for capturing and analyzing cells |
| US9707562B2 (en) | 2013-03-13 | 2017-07-18 | Denovo Sciences, Inc. | System for capturing and analyzing cells |
| US10391490B2 (en) | 2013-05-31 | 2019-08-27 | Celsee Diagnostics, Inc. | System and method for isolating and analyzing cells |
| US9856535B2 (en) | 2013-05-31 | 2018-01-02 | Denovo Sciences, Inc. | System for isolating cells |
| JP6506747B2 (ja) | 2013-06-25 | 2019-04-24 | ユニバーシティ オブ ワシントン スルー イッツ センター フォー コマーシャリゼーション | サンプル体積の自己デジタル処理 |
| WO2015048798A1 (en) | 2013-09-30 | 2015-04-02 | Gnubio, Inc. | Microfluidic cartridge device and methods of use and assembly |
| US20160271609A1 (en) * | 2013-10-03 | 2016-09-22 | The Trustees Of The University Of Pennsylvania | Highly parallelized droplet microfluidic apparatus |
| US11901041B2 (en) | 2013-10-04 | 2024-02-13 | Bio-Rad Laboratories, Inc. | Digital analysis of nucleic acid modification |
| US9889445B2 (en) | 2013-10-22 | 2018-02-13 | Berkeley Lights, Inc. | Micro-fluidic devices for assaying biological activity |
| EP3760703A1 (en) | 2013-10-22 | 2021-01-06 | Berkeley Lights, Inc. | Microfluidic devices having isolation pens and methods of testing biological micro-objects with same |
| US9944977B2 (en) | 2013-12-12 | 2018-04-17 | Raindance Technologies, Inc. | Distinguishing rare variations in a nucleic acid sequence from a sample |
| US11318479B2 (en) | 2013-12-18 | 2022-05-03 | Berkeley Lights, Inc. | Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device |
| JP6183471B2 (ja) * | 2014-01-31 | 2017-08-23 | 凸版印刷株式会社 | 生体分子解析キット及び生体分子解析方法 |
| US11192107B2 (en) | 2014-04-25 | 2021-12-07 | Berkeley Lights, Inc. | DEP force control and electrowetting control in different sections of the same microfluidic apparatus |
| JP2015211931A (ja) * | 2014-05-01 | 2015-11-26 | 学校法人同志社 | マイクロ流体チップを用いた微小液滴の製造法および微小液滴製造装置 |
| WO2015188171A1 (en) * | 2014-06-06 | 2015-12-10 | Berkeley Lights, Inc. | Isolating microfluidic structures and trapping bubbles |
| EP3229961B1 (en) | 2014-12-08 | 2019-11-13 | Berkeley Lights, Inc. | Actuated microfluidic structures for directed flow in a microfluidic device and methods of use thereof |
| CA2974632A1 (en) | 2015-02-04 | 2016-08-11 | The University Of British Columbia | Methods and devices for analyzing particles |
| CN107810059B (zh) | 2015-04-22 | 2021-03-23 | 伯克利之光生命科技公司 | 在微流体装置上冷冻和存档细胞 |
| US10751715B1 (en) | 2015-04-22 | 2020-08-25 | Berkeley Lights, Inc. | Microfluidic reporter cell assay methods and kits thereof |
| SG11201708429WA (en) | 2015-04-22 | 2017-11-29 | Berkeley Lights Inc | Microfluidic cell culture |
| JP6868289B2 (ja) | 2015-07-07 | 2021-05-12 | ユニバーシティ オブ ワシントンＵｎｉｖｅｒｓｉｔｙ ｏｆ Ｗａｓｈｉｎｇｔｏｎ | サンプルの自己デジタル化のためのシステム、方法、およびデバイス |
| US10647981B1 (en) | 2015-09-08 | 2020-05-12 | Bio-Rad Laboratories, Inc. | Nucleic acid library generation methods and compositions |
| US10799867B2 (en) | 2015-09-24 | 2020-10-13 | The Trustees Of The University Of Pennsylvania | Apparatus for generating microdroplets and methods of manufacturing |
| US10799865B2 (en) | 2015-10-27 | 2020-10-13 | Berkeley Lights, Inc. | Microfluidic apparatus having an optimized electrowetting surface and related systems and methods |
| CN108495712A (zh) | 2015-11-23 | 2018-09-04 | 伯克利之光生命科技公司 | 原位生成的微流体隔离结构、试剂盒及其使用方法 |
| US10705082B2 (en) | 2015-12-08 | 2020-07-07 | Berkeley Lights, Inc. | In situ-generated microfluidic assay structures, related kits, and methods of use thereof |
| US10206277B2 (en) * | 2015-12-18 | 2019-02-12 | Intel Corporation | Gradient encapsulant protection of devices in stretchable electronics |
| CA3009073C (en) | 2015-12-30 | 2024-11-12 | Berkeley Lights, Inc. | MICROFLUID DEVICES FOR OPTICALLY CONTROLLED CONVECTION AND MOVEMENT, KITS AND ASSOCIATED PROCESSES |
| WO2017117521A1 (en) | 2015-12-31 | 2017-07-06 | Berkeley Lights, Inc. | Tumor infilitrating cells engineered to express a pro-inflammatory polypeptide |
| EP3889176A1 (en) | 2016-01-15 | 2021-10-06 | Berkeley Lights, Inc. | Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor |
| CN108698003B (zh) * | 2016-02-19 | 2021-02-02 | 珀金埃尔默健康科学有限公司 | 微流体混合装置、微流体模块和方法 |
| CN109922885B (zh) | 2016-03-16 | 2022-05-10 | 伯克利之光生命科技公司 | 用于基因组编辑克隆的选择和传代的方法、系统和装置 |
| EP4684881A3 (en) | 2016-04-15 | 2026-03-18 | Bruker Cellular Analysis, Inc. | Methods, systems, computer program and non-transitory computer-readable medium for in-pen assays |
| US10675625B2 (en) | 2016-04-15 | 2020-06-09 | Berkeley Lights, Inc | Light sequencing and patterns for dielectrophoretic transport |
| CN115678773A (zh) | 2016-05-26 | 2023-02-03 | 伯克利之光生命科技公司 | 共价修饰的表面、试剂盒及制备方法和用途 |
| AU2017298545B2 (en) | 2016-07-21 | 2022-10-27 | Berkeley Lights, Inc. | Sorting of T lymphocytes in a microfluidic device |
| AU2017368267B2 (en) | 2016-12-01 | 2022-12-15 | Berkeley Lights, Inc. | Apparatuses, systems and methods for imaging micro-objects |
| CN110546495B (zh) | 2016-12-30 | 2022-11-01 | 加利福尼亚州立大学董事会 | 用于基因组编辑t细胞的选择和传代的方法 |
| AU2018243360B2 (en) * | 2017-03-29 | 2023-10-26 | Cornell University | Devices, processes, and systems for determination of nucleic acid sequence, expression, copy number, or methylation changes using combined nuclease, ligase, polymerase, and sequencing reactions |
| US10391492B2 (en) | 2017-08-29 | 2019-08-27 | Celsee Diagnostics, Inc. | System and method for isolating and analyzing cells |
| EP3721209B1 (en) | 2017-10-15 | 2024-02-07 | Berkeley Lights, Inc. | Methods for in-pen assays |
| DE102018200518B4 (de) * | 2018-01-15 | 2023-09-14 | Robert Bosch Gmbh | Mikrofluidischen Vorrichtung und Verfahren zu dessen Betrieb |
| CN108636465B (zh) * | 2018-05-15 | 2020-08-14 | 中国科学院化学研究所 | 图案化流体阵列及其制备方法和应用 |
| JP7526100B2 (ja) | 2018-05-31 | 2024-07-31 | バークレー ライツ，インコーポレイテッド | マイクロ流体デバイスによる微小物体の自動検出及び特徴付け |
| EP3790662B1 (en) * | 2018-06-21 | 2025-12-31 | Astraveus | MICROFLUID DEVICE AND PARTICLE TREATMENT METHOD |
| US11993766B2 (en) | 2018-09-21 | 2024-05-28 | Bruker Cellular Analysis, Inc. | Functionalized well plate, methods of preparation and use thereof |
| CN112189139B (zh) | 2018-09-26 | 2024-10-29 | 北卡罗来纳-查佩尔山大学 | 用于改进测定的化合物、组合物和方法 |
| WO2020077274A1 (en) | 2018-10-11 | 2020-04-16 | Berkeley Lights, Inc. | Systems and methods for identification of optimized protein production and kits therefor |
| CN113366312A (zh) | 2018-11-01 | 2021-09-07 | 伯克利之光生命科技公司 | 在微流体环境中测定生物细胞的方法 |
| WO2020168258A1 (en) | 2019-02-15 | 2020-08-20 | Berkeley Lights, Inc. | Laser-assisted repositioning of a micro-object and culturing of an attachment-dependent cell in a microfluidic environment |
| US11976269B2 (en) * | 2019-03-18 | 2024-05-07 | Cellular Research, Inc. | Precise delivery of components into fluids |
| US20220193678A1 (en) * | 2019-04-08 | 2022-06-23 | Technion Research And Development Foundation, Ltd. | Multiplexed array of nanoliter droplet array device |
| US10633693B1 (en) | 2019-04-16 | 2020-04-28 | Celsee Diagnostics, Inc. | System and method for leakage control in a particle capture system |
| WO2020223555A1 (en) | 2019-04-30 | 2020-11-05 | Berkeley Lights, Inc. | Methods for encapsulating and assaying cells |
| AU2020267743B2 (en) | 2019-05-07 | 2023-04-06 | Bio-Rad Laboratories, Inc. | System and method for automated single cell processing |
| US11273439B2 (en) | 2019-05-07 | 2022-03-15 | Bio-Rad Laboratories, Inc. | System and method for target material retrieval from microwells |
| CN111979087B (zh) * | 2019-05-22 | 2023-08-15 | 湖南乐准智芯生物科技有限公司 | 一种pcr微反应室芯片及其进样方法 |
| WO2020251802A1 (en) | 2019-06-14 | 2020-12-17 | Bio-Rad Laboratories, Inc. | System and method for automated single cell processing and analyses |
| US20220250069A1 (en) * | 2019-06-30 | 2022-08-11 | Nanosynex Ltd | Method for loading a multiplexed array of nanoliter droplet array devices |
| EP3996846A4 (en) * | 2019-07-09 | 2023-10-18 | Kryptos Biotechnologies, Inc. | Microfluidic reaction vessel array with patterned films |
| CN110665553B (zh) * | 2019-09-25 | 2021-11-09 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | 微滴检测进样系统及使用方法 |
| AU2020385015A1 (en) | 2019-11-17 | 2022-06-09 | Berkeley Lights, Inc. | Systems and methods for analyses of biological samples |
| US11504719B2 (en) | 2020-03-12 | 2022-11-22 | Bio-Rad Laboratories, Inc. | System and method for receiving and delivering a fluid for sample processing |
| WO2021201836A1 (en) * | 2020-03-31 | 2021-10-07 | Hewlett-Packard Development Company, L.P. | Microfluidic devices |
| CN115702240A (zh) * | 2020-06-24 | 2023-02-14 | 新加坡国立大学 | 用于菌落挑取的方法和系统 |
| WO2022051570A1 (en) | 2020-09-07 | 2022-03-10 | Berkeley Lights, Inc. | Methods of assaying a biological cell |
| CN118369438A (zh) | 2021-12-10 | 2024-07-19 | 伯乐实验室有限公司 | 用形态可调节功能化颗粒进行样品处理的组合物、方法和系统 |
| WO2023215974A1 (en) * | 2022-05-10 | 2023-11-16 | Moleculight Inc. | Fluorescence reporter and/or therapeutic systems, devices, and methods of use |
| CN118086053B (zh) * | 2024-04-29 | 2024-07-09 | 四川迪亚生物科技集团有限公司 | 一种自灌流培养板及使用方法 |
Family Cites Families (60)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6270961B1 (en) * | 1987-04-01 | 2001-08-07 | Hyseq, Inc. | Methods and apparatus for DNA sequencing and DNA identification |
| US5128247A (en) * | 1989-08-14 | 1992-07-07 | Board Of Regents, The University Of Texas System | Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources |
| US5130423A (en) * | 1990-07-13 | 1992-07-14 | Microprobe Corporation | Non-corrosive compositions and methods useful for the extraction of nucleic acids |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| CA2155186A1 (en) * | 1993-02-01 | 1994-08-18 | Kevin M. Ulmer | Methods and apparatus for dna sequencing |
| DK1921169T3 (da) | 1993-11-12 | 2012-06-04 | Phri Properties Inc | Hybridiseringssonder til nukleinsyredetektion, universelle stamceller, fremgangsmåder og kits |
| US6001229A (en) * | 1994-08-01 | 1999-12-14 | Lockheed Martin Energy Systems, Inc. | Apparatus and method for performing microfluidic manipulations for chemical analysis |
| KR19990022612A (ko) * | 1995-06-08 | 1999-03-25 | 퍼니스 챨스 엠 | 디엔에이 추출방법 및 추출장치 |
| US5856174A (en) * | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| US5945515A (en) * | 1995-07-31 | 1999-08-31 | Chomczynski; Piotr | Product and process for isolating DNA, RNA and proteins |
| CA2240667A1 (en) | 1995-12-18 | 1997-06-26 | Washington University | Method for nucleic acid analysis using fluorescence resonance energy transfer |
| DE69700499T2 (de) * | 1996-04-03 | 2000-03-23 | Perkin Elmer Corp | Vorrichtung und verfahren zum nachweis mehrerer analyten |
| US6825047B1 (en) * | 1996-04-03 | 2004-11-30 | Applera Corporation | Device and method for multiple analyte detection |
| US5942443A (en) * | 1996-06-28 | 1999-08-24 | Caliper Technologies Corporation | High throughput screening assay systems in microscale fluidic devices |
| CN1173776C (zh) | 1996-06-28 | 2004-11-03 | 卡钳技术有限公司 | 在微规模流体性设备里的高通过量的筛选分析系统 |
| JP3756313B2 (ja) * | 1997-03-07 | 2006-03-15 | 武 今西 | 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体 |
| EP1959255A3 (en) | 1997-04-04 | 2008-09-24 | Caliper Life Sciences, Inc. | Closed-loop biochemical analyzers |
| US6143496A (en) * | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
| US6794499B2 (en) * | 1997-09-12 | 2004-09-21 | Exiqon A/S | Oligonucleotide analogues |
| JP4236812B2 (ja) | 1997-09-12 | 2009-03-11 | エクシコン エ／エス | オリゴヌクレオチド類似体 |
| US6540895B1 (en) * | 1997-09-23 | 2003-04-01 | California Institute Of Technology | Microfabricated cell sorter for chemical and biological materials |
| GB9812768D0 (en) | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
| US6500650B1 (en) * | 1998-10-01 | 2002-12-31 | Variagenics, Inc. | Method for identifying polymorphisms |
| ATE556149T1 (de) * | 1999-02-23 | 2012-05-15 | Caliper Life Sciences Inc | Manipulation von mikropartikeln in mikrofluidischen systemen |
| DE60029314T2 (de) | 1999-03-24 | 2007-07-12 | Exiqon A/S | Verbesserte Synthese für -2.2.1. I Bicyclo-Nukleoside |
| US6555389B1 (en) * | 1999-05-11 | 2003-04-29 | Aclara Biosciences, Inc. | Sample evaporative control |
| US7601270B1 (en) * | 1999-06-28 | 2009-10-13 | California Institute Of Technology | Microfabricated elastomeric valve and pump systems |
| US7144616B1 (en) * | 1999-06-28 | 2006-12-05 | California Institute Of Technology | Microfabricated elastomeric valve and pump systems |
| US7244402B2 (en) * | 2001-04-06 | 2007-07-17 | California Institute Of Technology | Microfluidic protein crystallography |
| US6929030B2 (en) * | 1999-06-28 | 2005-08-16 | California Institute Of Technology | Microfabricated elastomeric valve and pump systems |
| US7217321B2 (en) * | 2001-04-06 | 2007-05-15 | California Institute Of Technology | Microfluidic protein crystallography techniques |
| US6899137B2 (en) * | 1999-06-28 | 2005-05-31 | California Institute Of Technology | Microfabricated elastomeric valve and pump systems |
| AU7406700A (en) | 1999-10-04 | 2001-05-10 | Exiqon A/S | Design of high affinity rnase h recruiting oligonucleotide |
| IL149694A0 (en) | 1999-12-23 | 2002-11-10 | Exiqon As | Therapeutic uses of lna-modified oligonucleotides |
| WO2001073127A2 (en) * | 2000-03-27 | 2001-10-04 | Applera Corporation | Improved invasion assay |
| US6481453B1 (en) * | 2000-04-14 | 2002-11-19 | Nanostream, Inc. | Microfluidic branch metering systems and methods |
| WO2001081631A1 (en) | 2000-04-25 | 2001-11-01 | Dna Sciences, Inc. | Detection of nucleotide sequence variations via the proofreading activity of polymerases |
| CN1232343C (zh) * | 2000-07-03 | 2005-12-21 | 艾克索特罗恩公司 | 利用光生反应物进行化学反应的设备及方法 |
| JP4413493B2 (ja) | 2000-10-04 | 2010-02-10 | サンタリス ファーマ アー／エス | プリンｌｎａ類似体の改善された合成方法 |
| EP1384022A4 (en) * | 2001-04-06 | 2004-08-04 | California Inst Of Techn | NUCLEIC ACID AMPLIFICATION USING MICROFLUID DEVICES |
| ATE287413T1 (de) | 2001-07-12 | 2005-02-15 | Santaris Pharma As | Verfahren zur herstellung des lna phosphoramidite |
| US7338760B2 (en) * | 2001-10-26 | 2008-03-04 | Ntu Ventures Private Limited | Sample preparation integrated chip |
| WO2003048295A1 (en) * | 2001-11-30 | 2003-06-12 | Fluidigm Corporation | Microfluidic device and methods of using same |
| US7312085B2 (en) * | 2002-04-01 | 2007-12-25 | Fluidigm Corporation | Microfluidic particle-analysis systems |
| AU2003224817B2 (en) * | 2002-04-01 | 2008-11-06 | Fluidigm Corporation | Microfluidic particle-analysis systems |
| US6872530B2 (en) * | 2002-04-24 | 2005-03-29 | Spectrumedix, Llc | Method for determining the presence of DNA variants using peptide nucleic acid probes |
| AU2003267960A1 (en) * | 2002-05-22 | 2004-01-06 | Marshall University | Methods, probes, and accessory molecules for detecting single nucleotide polymorphisms |
| JP2006501056A (ja) * | 2002-09-25 | 2006-01-12 | カリフォルニア インスティテュート オブ テクノロジー | ミクロ流体大規模集積 |
| ATE443765T1 (de) | 2003-03-21 | 2009-10-15 | Santaris Pharma As | Analoga kurzer interferierender rna (sirna) |
| CA2521171C (en) * | 2003-04-03 | 2013-05-28 | Fluidigm Corp. | Microfluidic devices and methods of using same |
| US7604965B2 (en) * | 2003-04-03 | 2009-10-20 | Fluidigm Corporation | Thermal reaction device and method for using the same |
| US20050164373A1 (en) * | 2004-01-22 | 2005-07-28 | Oldham Mark F. | Diffusion-aided loading system for microfluidic devices |
| WO2005080606A1 (en) * | 2004-02-18 | 2005-09-01 | Xiaochuan Zhou | Fluidic devices and methods for multiplex chemical and biochemical reactions |
| EP1796824A4 (en) * | 2004-05-02 | 2016-10-19 | Fluidigm Corp | DEVICE FOR THERMAL REACTIONS AND METHOD FOR THEIR USE |
| WO2006060748A2 (en) | 2004-12-03 | 2006-06-08 | California Institute Of Technology | Microfluidic sieve valves |
| WO2007044091A2 (en) * | 2005-06-02 | 2007-04-19 | Fluidigm Corporation | Analysis using microfluidic partitioning devices |
| CN101351542A (zh) * | 2005-07-15 | 2009-01-21 | 阿普尔拉公司 | 液体加工装置和方法 |
| US7915030B2 (en) | 2005-09-01 | 2011-03-29 | Canon U.S. Life Sciences, Inc. | Method and molecular diagnostic device for detection, analysis and identification of genomic DNA |
| WO2008063135A1 (en) * | 2006-11-24 | 2008-05-29 | Agency For Science, Technology And Research | Apparatus for processing a sample in a liquid droplet and method of using the same |
| US9211537B2 (en) | 2007-11-07 | 2015-12-15 | The University Of British Columbia | Microfluidic device and method of using same |
-
2008
- 2008-11-07 US US12/741,880 patent/US9211537B2/en not_active Expired - Fee Related
- 2008-11-07 CN CN200880124204.9A patent/CN101910415B/zh not_active Expired - Fee Related
- 2008-11-07 CA CA2705213A patent/CA2705213C/en not_active Expired - Fee Related
- 2008-11-07 EP EP08846830A patent/EP2212437A4/en not_active Withdrawn
- 2008-11-07 JP JP2010531389A patent/JP5404638B2/ja not_active Expired - Fee Related
- 2008-11-07 WO PCT/CA2008/001985 patent/WO2009059430A1/en not_active Ceased
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