JP2011109995A - Add-in material for mushroom cultivation, artificial culture medium for mushroom, and method for artificially cultivating mushroom using the culture medium - Google Patents
Add-in material for mushroom cultivation, artificial culture medium for mushroom, and method for artificially cultivating mushroom using the culture medium Download PDFInfo
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- JP2011109995A JP2011109995A JP2009271019A JP2009271019A JP2011109995A JP 2011109995 A JP2011109995 A JP 2011109995A JP 2009271019 A JP2009271019 A JP 2009271019A JP 2009271019 A JP2009271019 A JP 2009271019A JP 2011109995 A JP2011109995 A JP 2011109995A
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- alkaline earth
- earth metal
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- mushrooms
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- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 87
- 239000001963 growth medium Substances 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 26
- 239000000463 material Substances 0.000 title abstract description 7
- 229910052784 alkaline earth metal Inorganic materials 0.000 claims abstract description 39
- 150000001342 alkaline earth metals Chemical class 0.000 claims abstract description 39
- 229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 33
- 239000011521 glass Substances 0.000 claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 claims abstract description 19
- GDVKFRBCXAPAQJ-UHFFFAOYSA-A dialuminum;hexamagnesium;carbonate;hexadecahydroxide Chemical compound [OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Al+3].[Al+3].[O-]C([O-])=O GDVKFRBCXAPAQJ-UHFFFAOYSA-A 0.000 claims abstract description 17
- 229910001701 hydrotalcite Inorganic materials 0.000 claims abstract description 17
- 229960001545 hydrotalcite Drugs 0.000 claims abstract description 17
- 241000282485 Vulpes vulpes Species 0.000 claims abstract description 14
- 238000012360 testing method Methods 0.000 claims abstract description 14
- 239000007864 aqueous solution Substances 0.000 claims abstract description 9
- 239000002905 metal composite material Substances 0.000 claims description 32
- 239000000404 calcium aluminium silicate Substances 0.000 claims description 27
- 235000012215 calcium aluminium silicate Nutrition 0.000 claims description 27
- 229940078583 calcium aluminosilicate Drugs 0.000 claims description 27
- WNCYAPRTYDMSFP-UHFFFAOYSA-N calcium aluminosilicate Chemical compound [Al+3].[Al+3].[Ca+2].[O-][Si]([O-])=O.[O-][Si]([O-])=O.[O-][Si]([O-])=O.[O-][Si]([O-])=O WNCYAPRTYDMSFP-UHFFFAOYSA-N 0.000 claims description 26
- 239000000654 additive Substances 0.000 claims description 22
- 230000000996 additive effect Effects 0.000 claims description 20
- 229910000287 alkaline earth metal oxide Inorganic materials 0.000 claims description 19
- 229910001860 alkaline earth metal hydroxide Inorganic materials 0.000 claims description 18
- DIZPMCHEQGEION-UHFFFAOYSA-H aluminium sulfate (anhydrous) Chemical group [Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O DIZPMCHEQGEION-UHFFFAOYSA-H 0.000 claims description 9
- AZDRQVAHHNSJOQ-UHFFFAOYSA-N alumane Chemical group [AlH3] AZDRQVAHHNSJOQ-UHFFFAOYSA-N 0.000 claims description 6
- 230000002378 acidificating effect Effects 0.000 claims description 5
- 238000012258 culturing Methods 0.000 abstract description 3
- IQDXNHZDRQHKEF-UHFFFAOYSA-N dialuminum;dicalcium;dioxido(oxo)silane Chemical compound [Al+3].[Al+3].[Ca+2].[Ca+2].[O-][Si]([O-])=O.[O-][Si]([O-])=O.[O-][Si]([O-])=O.[O-][Si]([O-])=O.[O-][Si]([O-])=O IQDXNHZDRQHKEF-UHFFFAOYSA-N 0.000 abstract 1
- 230000002349 favourable effect Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 229910004298 SiO 2 Inorganic materials 0.000 description 16
- 229910018072 Al 2 O 3 Inorganic materials 0.000 description 15
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 15
- 239000002994 raw material Substances 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- 239000000292 calcium oxide Substances 0.000 description 12
- 239000003153 chemical reaction reagent Substances 0.000 description 12
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 description 11
- 239000002609 medium Substances 0.000 description 11
- 150000004679 hydroxides Chemical class 0.000 description 10
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 8
- 239000000395 magnesium oxide Substances 0.000 description 8
- CPLXHLVBOLITMK-UHFFFAOYSA-N magnesium oxide Inorganic materials [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 description 8
- AXZKOIWUVFPNLO-UHFFFAOYSA-N magnesium;oxygen(2-) Chemical compound [O-2].[Mg+2] AXZKOIWUVFPNLO-UHFFFAOYSA-N 0.000 description 8
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 7
- 235000017491 Bambusa tulda Nutrition 0.000 description 7
- 241001330002 Bambuseae Species 0.000 description 7
- 241000233866 Fungi Species 0.000 description 7
- 240000000599 Lentinula edodes Species 0.000 description 7
- 240000007594 Oryza sativa Species 0.000 description 7
- 235000007164 Oryza sativa Nutrition 0.000 description 7
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 7
- 239000011425 bamboo Substances 0.000 description 7
- 150000001875 compounds Chemical class 0.000 description 7
- 235000009566 rice Nutrition 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 description 6
- 239000002131 composite material Substances 0.000 description 6
- 239000002245 particle Substances 0.000 description 6
- 150000003839 salts Chemical class 0.000 description 6
- 239000002253 acid Chemical class 0.000 description 5
- 238000003306 harvesting Methods 0.000 description 5
- -1 aluminosilicate compound Chemical class 0.000 description 4
- 239000011575 calcium Substances 0.000 description 4
- 239000011777 magnesium Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000004017 vitrification Methods 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 235000001715 Lentinula edodes Nutrition 0.000 description 3
- 244000168667 Pholiota nameko Species 0.000 description 3
- 235000014528 Pholiota nameko Nutrition 0.000 description 3
- 239000002585 base Substances 0.000 description 3
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 3
- 239000000920 calcium hydroxide Substances 0.000 description 3
- 235000011116 calcium hydroxide Nutrition 0.000 description 3
- 229910001861 calcium hydroxide Inorganic materials 0.000 description 3
- XFWJKVMFIVXPKK-UHFFFAOYSA-N calcium;oxido(oxo)alumane Chemical class [Ca+2].[O-][Al]=O.[O-][Al]=O XFWJKVMFIVXPKK-UHFFFAOYSA-N 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 238000012364 cultivation method Methods 0.000 description 3
- 239000011121 hardwood Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- KLZUFWVZNOTSEM-UHFFFAOYSA-K Aluminium flouride Chemical compound F[Al](F)F KLZUFWVZNOTSEM-UHFFFAOYSA-K 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 240000000731 Fagus sylvatica Species 0.000 description 2
- 235000010099 Fagus sylvatica Nutrition 0.000 description 2
- 240000006499 Flammulina velutipes Species 0.000 description 2
- 235000016640 Flammulina velutipes Nutrition 0.000 description 2
- 240000001080 Grifola frondosa Species 0.000 description 2
- 235000007710 Grifola frondosa Nutrition 0.000 description 2
- 241000238413 Octopus Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 241000242583 Scyphozoa Species 0.000 description 2
- 229910000323 aluminium silicate Inorganic materials 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 235000012255 calcium oxide Nutrition 0.000 description 2
- 235000012241 calcium silicate Nutrition 0.000 description 2
- 229910052918 calcium silicate Inorganic materials 0.000 description 2
- RPUZVWKKWXPKIP-UHFFFAOYSA-H dialuminum;hydrogen phosphate Chemical compound [Al+3].[Al+3].OP([O-])([O-])=O.OP([O-])([O-])=O.OP([O-])([O-])=O RPUZVWKKWXPKIP-UHFFFAOYSA-H 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 238000006386 neutralization reaction Methods 0.000 description 2
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 235000012239 silicon dioxide Nutrition 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- IRPGOXJVTQTAAN-UHFFFAOYSA-N 2,2,3,3,3-pentafluoropropanal Chemical compound FC(F)(F)C(F)(F)C=O IRPGOXJVTQTAAN-UHFFFAOYSA-N 0.000 description 1
- 238000004438 BET method Methods 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- LTPBRCUWZOMYOC-UHFFFAOYSA-N Beryllium oxide Chemical compound O=[Be] LTPBRCUWZOMYOC-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 244000301850 Cupressus sempervirens Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 235000019738 Limestone Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000001462 Pleurotus ostreatus Species 0.000 description 1
- 235000001603 Pleurotus ostreatus Nutrition 0.000 description 1
- 239000011398 Portland cement Substances 0.000 description 1
- 239000006004 Quartz sand Substances 0.000 description 1
- 238000003991 Rietveld refinement Methods 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 229910010413 TiO 2 Inorganic materials 0.000 description 1
- 238000002441 X-ray diffraction Methods 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 150000008043 acidic salts Chemical class 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- SNAAJJQQZSMGQD-UHFFFAOYSA-N aluminum magnesium Chemical compound [Mg].[Al] SNAAJJQQZSMGQD-UHFFFAOYSA-N 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- RQPZNWPYLFFXCP-UHFFFAOYSA-L barium dihydroxide Chemical compound [OH-].[OH-].[Ba+2] RQPZNWPYLFFXCP-UHFFFAOYSA-L 0.000 description 1
- 229910001863 barium hydroxide Inorganic materials 0.000 description 1
- QVQLCTNNEUAWMS-UHFFFAOYSA-N barium oxide Chemical compound [Ba]=O QVQLCTNNEUAWMS-UHFFFAOYSA-N 0.000 description 1
- 229910001570 bauxite Inorganic materials 0.000 description 1
- 229910052790 beryllium Inorganic materials 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- JHLNERQLKQQLRZ-UHFFFAOYSA-N calcium silicate Chemical compound [Ca+2].[Ca+2].[O-][Si]([O-])([O-])[O-] JHLNERQLKQQLRZ-UHFFFAOYSA-N 0.000 description 1
- 239000000378 calcium silicate Substances 0.000 description 1
- OYACROKNLOSFPA-UHFFFAOYSA-N calcium;dioxido(oxo)silane Chemical compound [Ca+2].[O-][Si]([O-])=O OYACROKNLOSFPA-UHFFFAOYSA-N 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 239000010459 dolomite Substances 0.000 description 1
- 229910000514 dolomite Inorganic materials 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-M hydrogensulfate Chemical compound OS([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-M 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 229910000358 iron sulfate Inorganic materials 0.000 description 1
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 239000006028 limestone Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- VTHJTEIRLNZDEV-UHFFFAOYSA-L magnesium dihydroxide Chemical compound [OH-].[OH-].[Mg+2] VTHJTEIRLNZDEV-UHFFFAOYSA-L 0.000 description 1
- 239000000347 magnesium hydroxide Substances 0.000 description 1
- 229910001862 magnesium hydroxide Inorganic materials 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- UFQXGXDIJMBKTC-UHFFFAOYSA-N oxostrontium Chemical compound [Sr]=O UFQXGXDIJMBKTC-UHFFFAOYSA-N 0.000 description 1
- MKTRXTLKNXLULX-UHFFFAOYSA-P pentacalcium;dioxido(oxo)silane;hydron;tetrahydrate Chemical compound [H+].[H+].O.O.O.O.[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-][Si]([O-])=O.[O-][Si]([O-])=O.[O-][Si]([O-])=O.[O-][Si]([O-])=O.[O-][Si]([O-])=O.[O-][Si]([O-])=O MKTRXTLKNXLULX-UHFFFAOYSA-P 0.000 description 1
- CHKVPAROMQMJNQ-UHFFFAOYSA-M potassium bisulfate Chemical compound [K+].OS([O-])(=O)=O CHKVPAROMQMJNQ-UHFFFAOYSA-M 0.000 description 1
- 229910000343 potassium bisulfate Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000000634 powder X-ray diffraction Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical compound O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 229910052712 strontium Inorganic materials 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
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- Mushroom Cultivation (AREA)
Abstract
Description
本発明は、きのこの栽培用添加材、きのこの人工培養基、及びそれを用いたきのこの人工栽培方法に関する。 The present invention relates to an additive for cultivation of mushrooms, an artificial culture medium for mushrooms, and a method for artificial cultivation of mushrooms using the same.
従来、きのこの栽培は、くぬぎ、ぶな、及びならなどの原木を利用した、ほだ木栽培がほとんどであり、そのため気象条件により収穫が左右されることが多いという問題があった。また、ほだ木栽培では栽培期間が長く、種菌の接種からきのこの収穫までに１年半〜２年も要するので、生産コストが相当高くつくという問題もあった。 Conventionally, the cultivation of mushrooms has mostly been cultivating cypress trees using raw wood such as kunugi, beech, and nara. Therefore, there has been a problem that harvesting is often influenced by weather conditions. In addition, the cultivation period of the wood is long, and it takes one and a half to two years from the inoculation of the inoculum to the harvest of the mushrooms, so that the production cost is considerably high.
そこで近年、えのきたけ、ひらたけ、なめこ、及びしいたけなどは、例えば、鋸屑に米糠を配合した培地を用い、瓶又は箱で培養を行う菌床人工栽培方法が確立され、一年を通じて、四季に関係なく安定してこれらのきのこが収穫できるようになっている。
即ち、農家での副業的性格が強く、小規模生産に頼っていた従来のきのこの栽培が、現在では大規模専業生産が可能で、かつ、原料が入手しやすい菌床人工栽培方法に移りつつある。
ここで、培地とは、微生物あるいは動植物の組織等を培養するために調製された液状又は固形の物質で、培養基ともいわれている。
しかしながら、この菌床人工栽培においても、きのこを大量に連続栽培するには、いまだ収率も低く、かつ、栽培期間がかなり長いため、その生産コストは安価とは言えず、今後、これら生産性の改善が切望されている。
Therefore, in recent years, for example, enokitake, hiratake, nameko, shiitake, etc. have been established a method for artificial cultivation of fungus beds using a medium in which rice bran is mixed with sawdust and cultivated in bottles or boxes. Regardless of the situation, these mushrooms can be harvested stably.
In other words, the traditional mushroom cultivation, which has a strong side-business character at farmers and relies on small-scale production, is now shifting to a fungus bed artificial cultivation method that allows large-scale specialized production and is easy to obtain raw materials. is there.
Here, the culture medium is a liquid or solid substance prepared for culturing microorganisms or animal and plant tissues, and is also called a culture medium.
However, even in this fungal bed artificial cultivation, in order to continuously cultivate mushrooms in large quantities, the yield is still low and the cultivation period is quite long, so the production cost is not low, and in the future these productivity Improvement is eagerly desired.
生産性を改善する方法として培地に特定の化合物を配合する方法が知られている。例えば、培地に、アルミノシリケート系化合物（特許文献１）やマグネシウムアルミニウムシリケート系化合物（特許文献２や特許文献３）、アルミノケイ酸カルシウム系化合物（特許文献４、特許文献５、及び特許文献６）、カルシウムアルミネート系化合物（特許文献７や特許文献８）、並びに、カルシウムアルミネート系化合物と硫酸塩を併用したもの（特許文献７）などが挙げられる。 As a method for improving productivity, a method of blending a specific compound in a medium is known. For example, in the medium, an aluminosilicate compound (Patent Document 1), a magnesium aluminum silicate compound (Patent Document 2 or Patent Document 3), a calcium aluminosilicate compound (Patent Document 4, Patent Document 5, and Patent Document 6), Examples thereof include calcium aluminate compounds (Patent Document 7 and Patent Document 8), and those using calcium aluminate compounds and sulfates in combination (Patent Document 7).
一方、硫酸アルミニウムなどの硫酸塩とアルミノケイ酸カルシウムをきのこ栽培用培養基に使用することが提案されている（特許文献５）。
しかしながら、特許文献５には、きのこの栽培用添加材のｐＨを規定することについてはなんら記載がない。
On the other hand, it has been proposed to use a sulfate such as aluminum sulfate and calcium aluminosilicate as a culture medium for mushroom cultivation (Patent Document 5).
However, Patent Document 5 has no description about prescribing the pH of the additive for cultivation of mushrooms.
また、アルミノケイ酸カルシウムガラスと、アルカリ土類金属の酸化物、水酸化物、及び複合水酸化物からなる群から選ばれた一種又は二種以上を含有してなるきのこの人工培養基も提案されている（特許文献９）。
しかしながら、特許文献９には、きのこの栽培用添加材のｐＨを規定することについてはなんら記載がない。
Also proposed is an artificial culture medium for mushrooms containing one or more selected from the group consisting of calcium aluminosilicate glass and alkaline earth metal oxides, hydroxides, and composite hydroxides. (Patent Document 9).
However, Patent Document 9 has no description about prescribing the pH of the additive for cultivation of mushrooms.
本発明は、上記のような問題を解決しようとするものであり、形や大きさが良好なきのこを高い収率で、短期間で栽培できるきのこの栽培用添加材、きのこの人工培養基、及びそれを用いたきのこの人工栽培方法を提供することを課題とする。 The present invention is intended to solve the above-mentioned problems, and an additive for cultivating mushrooms that can be cultivated in a high yield and in a short period of time with a good shape and size, an artificial culture medium for mushrooms, and It aims at providing the artificial cultivation method of the mushroom using it.
本発明は、（１）水溶液が酸性を示す無機塩と、アルミノケイ酸カルシウムガラス、アルカリ土類金属の酸化物、アルカリ土類金属の複合酸化物、アルカリ土類金属の水酸化物、アルカリ土類金属の複合水酸化物、及びハイドロタルサイトからなる群から選ばれた一種又は二種以上とを含有してなり、Fuchs法試験で測定した最高ｐＨが3.5〜4.5であることを特徴とするきのこの栽培用添加材、（２）前記無機塩が、アルミニウム塩であることを特徴とする（１）のきのこの栽培用添加材、（３）前記アルミニウム塩が、硫酸アルミニウムであることを特徴とする（２）のきのこの栽培用添加材、（４）Fuchs法試験で測定したｐＨ3.0までの到達時間が120秒以内であることを特徴とする（１）〜（３）のいずれか一のきのこの栽培用添加材、（５）（１）〜（４）のいずれか一のきのこの栽培用添加材を含有してなるきのこの人工培養基、（６）（５）のきのこの人工培養基を用いてなるきのこの人工栽培方法である。 The present invention includes (1) an inorganic salt in which an aqueous solution is acidic, calcium aluminosilicate glass, an alkaline earth metal oxide, an alkaline earth metal composite oxide, an alkaline earth metal hydroxide, an alkaline earth A mushroom characterized in that it contains one or more selected from the group consisting of metal composite hydroxides and hydrotalcite, and has a maximum pH of 3.5 to 4.5 measured by the Fuchs method test. (1) The additive for cultivating mushrooms, wherein the inorganic salt is an aluminum salt, (3) The aluminum salt is aluminum sulfate, (2) Mushroom Additives for Cultivation, (4) Any one of (1) to (3) characterized in that the time to reach pH 3.0 measured by the Fuchs method test is within 120 seconds Additives for cultivation of mushrooms, (5) ( A mushroom artificial culture medium comprising the mushroom cultivation additive of any one of 1) to (4), and a mushroom artificial cultivation method using the mushroom artificial culture medium of (6) and (5).
本発明のきのこの人工培養基を使用すると、形や大きさが良好なきのこを高い収率で栽培できる。 When the artificial culture medium of the mushroom of the present invention is used, mushrooms having a good shape and size can be cultivated with a high yield.
なお、本発明で使用する部や％は、特に規定のない限り質量基準である。 The parts and% used in the present invention are based on mass unless otherwise specified.
本発明で使用する人工培養基とは、鋸屑、コーンコブ、ふすま、及びもみがらなどの炭素源と、米糠や大豆粕等の窒素源とを主体とする培地と、水溶液が酸性を示す無機塩と、アルミノケイ酸カルシウムガラス、アルカリ土類金属の酸化物、アルカリ土類金属の複合酸化物、アルカリ土類金属の水酸化物、アルカリ土類金属の複合水酸化物、及びハイドロタルサイトからなる群から選ばれた一種又は二種以上とを含有してなり、Fuchs法試験で測定した最高ｐＨが3.5〜4.5であるきのこの栽培用添加材とを必須成分とするものである。これらの混合物に水を適当量加え、これを瓶又は箱に圧詰めしてきのこの人工培養基とする。 The artificial culture medium used in the present invention is a medium mainly composed of a carbon source such as sawdust, corn cob, bran and rice bran, a nitrogen source such as rice bran and soybean meal, and an inorganic salt in which the aqueous solution exhibits acidity, Selected from the group consisting of calcium aluminosilicate glass, alkaline earth metal oxide, alkaline earth metal composite oxide, alkaline earth metal hydroxide, alkaline earth metal composite hydroxide, and hydrotalcite The mushroom cultivation additive having a maximum pH of 3.5 to 4.5 measured by the Fuchs method test is an essential component. An appropriate amount of water is added to these mixtures, which are packed into bottles or boxes to form mushroom artificial culture media.
培地を調製するための、炭素源の量、窒素源の量、及び水量は、その種類や含有水分量等により変化し、一義的に決定されるものではない。例えば、炭素源として鋸屑を用いる場合、鋸屑は、乾燥状態（乾物量）で培地全乾物量の20〜90％程度の範囲で使用することができるが、この量は窒素源として用いる培地成分によって変動する。例えば、窒素源に米糠を使用する場合、鋸屑は、乾物量で培地全乾物量の40〜70％程度が好ましい。また、鋸屑を、乾物量で培地全乾物量の20〜90％の範囲で使用する場合、鋸屑と米糠を質量比１：１の割合で混合した混合物に水を加えて、含水率を55〜70％に調整したものを、瓶又は箱に圧詰めして調製することが好ましい。また、鋸屑としては広葉樹鋸屑あるいは針葉樹鋸屑をそれぞれ単独あるいは混合して使用することも可能である。 The amount of the carbon source, the amount of the nitrogen source, and the amount of water for preparing the medium vary depending on the type and the amount of water contained therein, and are not uniquely determined. For example, when sawdust is used as a carbon source, the sawdust can be used in a dry state (dry matter amount) in a range of about 20 to 90% of the total dry matter amount of the medium. fluctuate. For example, when rice bran is used as the nitrogen source, the sawdust is preferably about 40 to 70% of the total amount of dry matter in terms of dry matter. In addition, when sawdust is used in a dry matter amount in the range of 20 to 90% of the total dry matter of the medium, water is added to a mixture in which sawdust and rice bran are mixed at a mass ratio of 1: 1 to obtain a moisture content of 55 to 55%. What is adjusted to 70% is preferably prepared by crushing into a bottle or box. Moreover, as sawdust, hardwood sawdust or coniferous sawdust can be used alone or in combination.
本発明で使用するきのこの栽培用添加材とは、水溶液が酸性を示す無機塩と、アルミノケイ酸カルシウムガラス、アルカリ土類金属の酸化物、アルカリ土類金属の複合酸化物、アルカリ土類金属の水酸化物、アルカリ土類金属の複合水酸化物、及びハイドロタルサイトからなる群から選ばれた一種又は二種以上とを含有してなり、Fuchs法試験で測定した最高ｐＨが3.5〜4.5であるものである。 The additive for cultivation of mushrooms used in the present invention includes an inorganic salt whose aqueous solution is acidic, calcium aluminosilicate glass, an alkaline earth metal oxide, an alkaline earth metal composite oxide, an alkaline earth metal It contains one or more selected from the group consisting of hydroxide, alkaline earth metal complex hydroxide, and hydrotalcite, and the maximum pH measured in the Fuchs method test is 3.5 to 4.5. There is something.
本発明で使用する水溶液が酸性を示す無機塩（以下、本無機塩という）とは、加水分解によって、例えば、ｐＨ４以上、７未満程度の酸性を示す塩を指し、正塩と酸性塩に分類される。
ここで、水溶液のｐＨとは、20℃の環境下で水１リットルに対して本無機塩50ｇを溶解したときのｐＨをいう。
水溶液が酸性を示す正塩とは、強酸と弱塩基の中和反応によって生成する塩を指し、例えば、塩化鉄（FeCl3）、硫酸アルミニウム（Al2(SO4)3）、硫酸鉄（II）（FeSO4）、塩化アルミニウム（AlCl3）、及びフッ化アルミニウム（AlF3）などが挙げられる。
一方、水溶液が酸性を示す酸性塩とは、強酸と強塩基の中和反応、もしくは、中程度の酸と強塩基の中和反応によって生成する酸のHを含む塩を指し、例えば、硫酸水素ナトリウム（NaHSO4）、硫酸水素カリウム（KHSO4）、リン酸二水素ナトリウム（NaH2PO4）、及びリン酸水素アルミニウム（Al2(HPO4)3）などが挙げられる。
本無機塩としては、性能面からアルミニウム塩が有効であり、コスト面からもアルミニウム塩が好ましく、例えば、硫酸アルミニウム（Al2(SO4)3）や塩化アルミニウム（AlCl3）などが挙げられ、中でもコスト面から硫酸アルミニウムが好ましい。
硫酸アルミニウムは、無水のものや結晶水を持ったものがあり、いずれも使用可能であるが、無水硫酸アルミニウムは粉末度が細かく、人工培養基に対して分散性が良いため好ましい。
本無機塩の粒度は、きのこの品質と収率の向上の面や、粉砕動力の面から、ブレーン法による比表面積値（以下、ブレーン比表面積値という）で2,000〜10,000cm2/gが好ましい。
The inorganic salt in which the aqueous solution used in the present invention exhibits acidity (hereinafter referred to as the present inorganic salt) refers to a salt that exhibits acidity of, for example, about pH 4 or more and less than 7 by hydrolysis, and is classified into a normal salt and an acid salt. Is done.
Here, the pH of the aqueous solution refers to the pH when 50 g of the present inorganic salt is dissolved in 1 liter of water in an environment of 20 ° C.
The normal salt in which an aqueous solution is acidic refers to a salt formed by a neutralization reaction between a strong acid and a weak base. For example, iron chloride (FeCl 3 ), aluminum sulfate (Al 2 (SO 4 ) 3 ), iron sulfate (II ) (FeSO 4 ), aluminum chloride (AlCl 3 ), and aluminum fluoride (AlF 3 ).
On the other hand, an acidic salt in which an aqueous solution is acidic refers to a salt containing H of an acid generated by a neutralization reaction between a strong acid and a strong base or a neutral acid and a strong base. For example, hydrogen sulfate Examples include sodium (NaHSO 4 ), potassium hydrogen sulfate (KHSO 4 ), sodium dihydrogen phosphate (NaH 2 PO 4 ), and aluminum hydrogen phosphate (Al 2 (HPO 4 ) 3 ).
As the inorganic salt, an aluminum salt is effective from the viewpoint of performance, and an aluminum salt is preferable from the viewpoint of cost. Examples thereof include aluminum sulfate (Al 2 (SO 4 ) 3 ) and aluminum chloride (AlCl 3 ). Of these, aluminum sulfate is preferable from the viewpoint of cost.
There are anhydrous aluminum sulfate and those with water of crystallization, and any of them can be used. However, anhydrous aluminum sulfate is preferable because it has a fine powder and good dispersibility with respect to an artificial culture medium.
The particle size of the inorganic salt is preferably 2,000 to 10,000 cm 2 / g in terms of the specific surface area value by the brane method (hereinafter referred to as the “brane specific surface area value”) from the viewpoint of improving the quality and yield of mushrooms and the power of grinding. .
本発明で使用するアルミノケイ酸カルシウムガラスとは、CaO原料、Al2O3原料、及びSiO2原料を電気炉や高周波炉等で加熱溶融したもので、CaO原料としては、生石灰、消石灰、及び石灰石等が使用でき、Al2O3原料としては、アルミナやボーキサイトなどが使用でき、SiO2原料としては、ケイ石、ケイ砂、及び石英等が使用できる。 The calcium aluminosilicate glass used in the present invention is obtained by heating and melting a CaO raw material, an Al 2 O 3 raw material, and a SiO 2 raw material in an electric furnace, a high frequency furnace, etc., and as the CaO raw material, quick lime, slaked lime, and limestone As the Al 2 O 3 raw material, alumina or bauxite can be used, and as the SiO 2 raw material, silica, quartz sand, quartz, or the like can be used.
なお、これら原料中には、MgO、TiO2、Fe2O3、Na2O、及びK2Oなどの不純物が含まれているが、本発明の効果を阻害しない範囲であれば許容されるものである。 These raw materials contain impurities such as MgO, TiO 2 , Fe 2 O 3 , Na 2 O, and K 2 O, but are acceptable as long as the effects of the present invention are not impaired. Is.
きのこの品質や収率の向上等の面から、アルミノケイ酸カルシウムガラスのCaO含有率は30〜60％、Al2O3含有率は10〜65％、SiO2含有率は５〜45％が好ましく、CaO含有率は40〜60％、Al2O3含有率は10〜40％、SiO2含有率は10〜40％がより好ましい。 From the aspect of improving the quality and yield of mushrooms, the CaO content of the calcium aluminosilicate glass is preferably 30-60%, the Al 2 O 3 content is 10-65%, and the SiO 2 content is preferably 5-45%. More preferably, the CaO content is 40-60%, the Al 2 O 3 content is 10-40%, and the SiO 2 content is 10-40%.
本発明のアルミノケイ酸カルシウムガラスのガラス化率は、きのこの品質や収率の向上の面で、50％以上が好ましい。 The vitrification rate of the calcium aluminosilicate glass of the present invention is preferably 50% or more from the viewpoint of improving the quality and yield of the mushroom.
ガラス化率の測定方法は、例えば、下記に示すエックス線回折図形を使用したリートベルト解析によって行うことができる。
即ち、粉砕した試料に酸化アルミニウムや酸化マグネシウムなどの内部標準物質を所定量添加し、めのう乳鉢等で充分混合したのち、粉末Ｘ線回折測定を実施する。測定結果を定量ソフトで解析し、ガラス化率を求める。定量ソフトには、Sietronics社製の「SIROQUANT」などを用いることが可能である。
The measuring method of vitrification rate can be performed by Rietveld analysis using the X-ray diffraction pattern shown below, for example.
That is, a predetermined amount of an internal standard substance such as aluminum oxide or magnesium oxide is added to the pulverized sample, and after sufficient mixing in an agate mortar or the like, powder X-ray diffraction measurement is performed. Analyze the measurement results with quantitative software to determine the vitrification rate. As the quantitative software, “SIROQUANT” manufactured by Sietronics can be used.
本発明で使用するアルミノケイ酸カルシウムガラスの粒度は、きのこの品質と収率の向上の面や、粉砕動力の面から、ブレーン比表面積値で2,000〜10,000cm2/gが好ましい。 The particle size of the calcium aluminosilicate glass used in the present invention is preferably 2,000 to 10,000 cm 2 / g in terms of Blaine specific surface area from the viewpoint of improving the quality and yield of mushrooms and from the viewpoint of grinding power.
本発明で使用するアルカリ土類金属の酸化物としては、酸化ベリリウム（BeO）、酸化マグネシウム（MgO）、酸化カルシウム（CaO）、酸化ストロンチウム（SrO）、及び酸化バリウム（BaO）などが挙げられ、アルカリ土類金属の複合酸化物としては、カルシウムシリケート（例えば、2CaO・SiO2や3CaO・SiO2）やポルトランドセメントなどが挙げられる。この中でも、きのこの品質、収率の向上、及び経済性の面から、酸化マグネシウム（MgO）や酸化カルシウム（CaO）のアルカリ土類金属の酸化物を用いることが好ましい。
アルカリ土類金属の酸化物やアルカリ土類金属の複合酸化物の粒度は、ブレーン比表面積値で2,000〜10,000cm2/gが好ましい。
Examples of the alkaline earth metal oxide used in the present invention include beryllium oxide (BeO), magnesium oxide (MgO), calcium oxide (CaO), strontium oxide (SrO), and barium oxide (BaO). Examples of the alkaline earth metal composite oxide include calcium silicate (for example, 2CaO · SiO 2 and 3CaO · SiO 2 ) and Portland cement. Among these, it is preferable to use an alkaline earth metal oxide such as magnesium oxide (MgO) or calcium oxide (CaO) from the viewpoint of mushroom quality, yield improvement, and economic efficiency.
The particle size of the alkaline earth metal oxide or the alkaline earth metal composite oxide is preferably 2,000 to 10,000 cm 2 / g in terms of Blaine specific surface area.
本発明で使用するアルカリ土類金属の水酸化物、アルカリ土類金属の複合水酸化物には、結晶質又は非晶質のものがあり、いずれも使用可能である。
結晶質のアルカリ土類金属の水酸化物としては、Ca(OH)2やMg(OH)2などがあり、結晶質のアルカリ土類金属の複合水酸化物としては、2CaO-Al2O3-SiO2-8H2OなどのCaO-Al2O3-SiO2-H2O系化合物、2CaO-Al2O3-8H2OなどのCaO-Al2O3-H2O系化合物、及び5CaO-6SiO2-5H2O（トバモライト）などのCaO-SiO2-H2O系化合物等がある。Caの部分が、Be、Mg、Sr、又はBaで置き換わった水酸化物や複合水酸化物も使用可能であり、きのこの品質や収率の向上と経済性の面からMgやCaを用いることが好ましい。
なお、アルカリ土類金属の水酸化物の粒度は、ブレーン比表面積値で2,000〜10,000cm2/gが好ましい。
また、アルカリ土類金属の複合水酸化物の粒度は、ＢＥＴ法による比表面積（以下、ＢＥＴ比表面積値という）で0.5〜20m2/gが好ましい。
The alkaline earth metal hydroxide and alkaline earth metal composite hydroxide used in the present invention may be crystalline or amorphous, and any of them can be used.
Crystalline alkaline earth metal hydroxides include Ca (OH) 2 and Mg (OH) 2 , and crystalline alkaline earth metal complex hydroxides include 2CaO-Al 2 O 3 -SiO 2 -8H 2 O CaO-Al 2 O 3 -SiO 2 -H 2 O -based compound such as, 2CaO-Al 2 O 3 -8H 2 O CaO-Al 2 O 3 -H 2 O -based compounds such as, And CaO—SiO 2 —H 2 O compounds such as 5CaO-6SiO 2 -5H 2 O (tobermorite). It is also possible to use hydroxides or composite hydroxides in which Ca is replaced by Be, Mg, Sr, or Ba. Use Mg or Ca from the standpoint of improving the quality and yield of mushrooms and economically. Is preferred.
The particle size of the alkaline earth metal hydroxide is preferably 2,000 to 10,000 cm 2 / g in terms of Blaine specific surface area.
The particle size of the alkaline earth metal composite hydroxide is preferably 0.5 to 20 m 2 / g in terms of the specific surface area (hereinafter referred to as BET specific surface area value) by the BET method.
また、本発明では、アルカリ土類金属の複合水酸化物として、アルカリ土類金属の水酸化物や複合水酸化物が炭酸化された物質も使用可能であり、例として、塩基性炭酸マグネシウムやドロマイトなどが挙げられる。 Further, in the present invention, alkaline earth metal hydroxides or substances obtained by carbonated composite hydroxides can be used as alkaline earth metal composite hydroxides. Examples include dolomite.
複合水酸化物の組成比は特に限定されるものではないが、きのこの品質や収率の向上の面から、CaOなどのアルカリ土類金属酸化物の含有率は10〜60％、Al2O3の含有率は０〜50％、SiO2の含有率は０〜50％、H2Oの含有率は10〜50％が好ましい。 The composition ratio of the composite hydroxide is not particularly limited, but from the viewpoint of improving the quality and yield of the mushroom, the content of alkaline earth metal oxides such as CaO is 10 to 60%, Al 2 O The content of 3 is preferably 0 to 50%, the content of SiO 2 is preferably 0 to 50%, and the content of H 2 O is preferably 10 to 50%.
これらの複合水酸化物は、水酸化カルシウム、水酸化マグネシウム、水酸化バリウム、水酸化アルミニウム、シリカゾル、及びシリカゲルなどを原料として水熱合成しても良いし、カルシウムアルミネート化合物のように水和活性を示すものは、CaOとAl2O3を焼成し、水と反応させて複合水酸化物を合成しても構わない。 These composite hydroxides may be hydrothermally synthesized using calcium hydroxide, magnesium hydroxide, barium hydroxide, aluminum hydroxide, silica sol, and silica gel as raw materials, or hydrated like calcium aluminate compounds. Those exhibiting activity may synthesize CaO and Al 2 O 3 and react with water to synthesize a composite hydroxide.
本発明で使用するハイドロタルサイトは、一般式が、［M2+ ６-6xM3+ 6ｘ(OH)12］6x+(An-)6x/n・yH2O（但し、M2+はMg2+とZn2+などの二価金属イオン、M3+はAl3+などの三価金属イオン、An-はCO3 2-などのn価の陰イオン、ｎはｎ≧1、ｘは0.1≦ｘ≦0.5、ｙはｙ≧０）で表される化合物である。
ハイドロタルサイトの粒度は、ＢＥＴ比表面積値で0.5〜20m2/gが好ましい。
The hydrotalcite used in the present invention has a general formula of [M 2+ 6-6x M 3+ 6x (OH) 12 ] 6x + (A n− ) 6x / n · yH 2 O (where M 2+ is mg 2+ and Zn divalent metal ions such as 2+, M 3+ is a trivalent metal ion, a n-is CO 3 2-n-valent anion, such as such as Al 3+, n is n ≧ 1, x is a compound represented by 0.1 ≦ x ≦ 0.5 and y is y ≧ 0).
The particle size of the hydrotalcite is preferably 0.5 to 20 m 2 / g as a BET specific surface area value.
本発明で使用するきのこの栽培用添加材は、本無機塩と、アルミノケイ酸カルシウムガラス、アルカリ土類金属の酸化物、アルカリ土類金属の複合酸化物、アルカリ土類金属の水酸化物、アルカリ土類金属の複合水酸化物、及びハイドロタルサイトからなる群から選ばれた一種又は二種以上を含有してなるものである。
本無機塩と、アルミノケイ酸カルシウムガラス、アルカリ土類金属の酸化物、アルカリ土類金属の複合酸化物、アルカリ土類金属の水酸化物、アルカリ土類金属の複合水酸化物、及びハイドロタルサイトからなる群から選ばれた一種又は二種以上の配合量は、培地や栽培するきのこの種類によって変化するため特に限定されるものではないが、通常、Fuchs法試験で測定した最高ｐＨと到達時間によって決められる。
The additive for cultivation of mushrooms used in the present invention includes the present inorganic salt, calcium aluminosilicate glass, alkaline earth metal oxide, alkaline earth metal composite oxide, alkaline earth metal hydroxide, alkali It contains one or more selected from the group consisting of complex hydroxides of earth metals and hydrotalcite.
The inorganic salt, calcium aluminosilicate glass, alkaline earth metal oxide, alkaline earth metal composite oxide, alkaline earth metal hydroxide, alkaline earth metal composite hydroxide, and hydrotalcite The amount of one or more selected from the group consisting of is not particularly limited because it varies depending on the medium and the type of mushrooms to be cultivated, but usually the maximum pH and the arrival time measured in the Fuchs method test It is decided by.
Fuchs法試験とは、0.1規定の塩酸50mlをビーカーにとり、１ｇのきのこの栽培用添加材を加えて撹拌し、ｐＨを測定（最初のｐＨ）し、撹拌開始から10分後以降、１規定の塩酸２mlを10分おきに加え、ｐＨを読み取り、最初のｐＨになるまで続ける方法であり、最初のｐＨからｐＨが3.0に到達するまでの時間（到達時間）と、到達時間以降の最高ｐＨによって、きのこの品質や収率を評価するものである。 Fuchs method test is to take 50 ml of 0.1N hydrochloric acid in a beaker, add 1g mushroom cultivation additive, stir, measure pH (initial pH), and after 10 minutes from the start of stirring, This is a method of adding 2 ml of hydrochloric acid every 10 minutes, reading the pH, and continuing until it reaches the first pH, depending on the time from the first pH until the pH reaches 3.0 (arrival time) and the maximum pH after the arrival time. The quality and yield of mushrooms are evaluated.
本発明のきのこの栽培用添加材は、きのこの品質や収率の向上の面から、Fuchs法試験で測定した最高ｐＨが3.5〜4.5であることが好ましい。最高ｐＨを3.5〜4.5にするために、きのこの栽培用添加材の成分割合や、各成分の化学組成を変えることなどが可能である。 From the aspect of improving the quality and yield of the mushroom, the additive for cultivation of mushrooms of the present invention preferably has a maximum pH of 3.5 to 4.5 measured by the Fuchs method test. In order to adjust the maximum pH to 3.5 to 4.5, it is possible to change the component ratio of the additive for cultivation of mushrooms or the chemical composition of each component.
また、Fuchs法試験で測定した到達時間は、きのこの品質や収率の向上の面から、15分以内が好ましく、120秒以内がより好ましい。 The arrival time measured by the Fuchs method test is preferably within 15 minutes, more preferably within 120 seconds, from the viewpoint of improving the quality of the mushroom and the yield.
本無機塩、アルミノケイ酸カルシウムガラス、アルカリ土類金属の酸化物、アルカリ土類金属の複合酸化物、アルカリ土類金属の水酸化物、アルカリ土類金属の複合水酸化物、及びハイドロタルサイトの使用量は、培地や栽培するきのこの種類によって変化するため特に限定されるものではなく、Fuchs法試験で測定した最高ｐＨによるが、通常、人工培養基1,000部に対して、本無機塩を１〜５部、アルミノケイ酸カルシウムガラス、アルカリ土類金属の酸化物、アルカリ土類金属の複合酸化物、アルカリ土類金属の水酸化物、アルカリ土類金属の複合水酸化物、及びハイドロタルサイトからなる群から選ばれた一種又は二種以上を１〜５部併用することが好ましい。 The inorganic salt, calcium aluminosilicate glass, alkaline earth metal oxide, alkaline earth metal composite oxide, alkaline earth metal hydroxide, alkaline earth metal composite hydroxide, and hydrotalcite The amount to be used is not particularly limited because it varies depending on the type of medium and mushrooms to be cultivated, and depends on the maximum pH measured in the Fuchs method test. 5 parts, calcium aluminosilicate glass, alkaline earth metal oxide, alkaline earth metal composite oxide, alkaline earth metal hydroxide, alkaline earth metal composite hydroxide, and hydrotalcite It is preferable to use 1 to 5 parts in combination of one or more selected from the group.
きのこの栽培用添加材の成分のそれぞれは、従来、きのこの収率を向上させる目的で使用されているが、両者を併用することによって相乗効果を呈し、きのこの品質や収率が大幅に向上する。 Each component of the additive for cultivation of mushrooms has been used for the purpose of improving the yield of mushrooms, but by using them together, a synergistic effect is exhibited, and the quality and yield of mushrooms are greatly improved. To do.
本発明の人工培養基を用いてきのこを栽培する方法は、各々の環境や状況等に応じて任意に変えることができるので特に限定されるものではないが、通常、本発明のきのこの栽培用添加材を含有した人工培養基に水を加えて、人工培養基の水分含有量を50〜70％に調整し、必要に応じて殺菌・冷却後、菌を接種し、各々のきのこについて通常採用されている培養工程や生育条件に従って行うことが好ましい。
例えば、ぶなしめじ栽培の場合は、菌を接種した人工培養基を22〜26℃で30〜40日間培養後、40〜50日間、培養とほぼ同条件で熟成し、菌かき後に温度14〜17℃、湿度95〜100％で20〜25日間育成を行って、ぶなしめじを栽培し収穫する。
また、しいたけ栽培の場合は、菌を接種した人工培養基を20〜25℃で約30日間培養後、26〜30℃で40〜50日間熟成し、その後、温度13〜17℃で１〜３日間低温処理し、温度17〜20℃、湿度90〜95％で約10日間発生を行ってしいたけを収穫し、この際に第１回目の収穫後に再び発生にかけて第２回目のしいたけの収穫を行うことも可能である。
The method for cultivating mushrooms using the artificial culture medium of the present invention is not particularly limited because it can be arbitrarily changed according to each environment, situation, etc., but usually the addition of mushroom cultivation of the present invention Water is added to the artificial culture medium containing the material, the water content of the artificial culture medium is adjusted to 50-70%, sterilized and cooled as necessary, and then inoculated with bacteria, which is usually adopted for each mushroom It is preferable to carry out according to the culture process and growth conditions.
For example, in the case of Bunameji cultivation, the artificial culture medium inoculated with the fungus is cultured at 22-26 ° C. for 30-40 days, then matured for 40-50 days under almost the same conditions as the culture, and after the fungus is scraped, the temperature is 14-17 ° C. Growing for 20-25 days at a humidity of 95-100%, cultivating and harvesting bamboo shoots.
In the case of Shiitake cultivation, the artificial culture medium inoculated with the fungus is cultured at 20-25 ° C. for about 30 days, then aged at 26-30 ° C. for 40-50 days, and then at a temperature of 13-17 ° C. for 1-3 days. Harvest shiitake mushrooms that have been treated at a low temperature for about 10 days at a temperature of 17 to 20 ° C and a humidity of 90 to 95%. At this time, the second shiitake is harvested again after the first harvest. Is also possible.
本発明で栽培されるきのこは人工栽培できるきのこであり、例えば、えのきたけ、ひらたけ、なめこ、ぶなしめじ、まいたけ、きくらげ、さるのこしかけ、及びしいたけなどが挙げられる。 The mushrooms cultivated in the present invention are mushrooms that can be artificially cultivated, and examples thereof include enokitake mushrooms, octopus mushrooms, nameko mushrooms, bean mushrooms, maitake mushrooms, jellyfish mushrooms, monkey mushrooms, and shiitake mushrooms.
以下、実験例に基づいて、本発明をさらに詳細に説明するが、本発明はこれらに限定されるものではない。 Hereinafter, the present invention will be described in more detail based on experimental examples, but the present invention is not limited thereto.
実験例１
CaO原料、Al2O3原料、及びSiO2原料を所定の割合で混合し、高周波炉で溶融後急冷却し、アルミノケイ酸カルシウムガラスを合成し、粉砕してブレーン比表面積値6,000cm2/gとした。
一方、広葉樹鋸屑250g、針葉樹鋸屑250g、米糠500g、及び水140mlからなる培地を調製し、培地1,000部に対して、表１に示す無機塩とアルミノケイ酸カルシウムガラスを配合した後、混合し、プラスチック製850ml広口瓶に圧詰めした。
その後、120℃飽和蒸気圧下で加熱殺菌後、ぶなしめじの菌を植え付け、暗所、温度25℃、湿度55％の条件下で２ヶ月間かけて菌を培養した。
菌の培養後、広口瓶の中央に直径１cm程度の穴を開け（菌かき）、充分に吸水させた後、15℃、湿度95％、及び照度20ルックスの条件下で４日間培養して子実体原基を形成し、照度を200ルックスに上げて２週間程度培養を続け、子実体の収量を測定し、コントロール対比と品質を評価した。
なお、無機塩だけを用いた場合、アルミノケイ酸カルシウムガラスだけを用いた場合、両方とも用いなかった場合を比較例とした。結果を表１に併記する。
Experimental example 1
CaO raw material, Al 2 O 3 raw material, and SiO 2 raw material are mixed at a predetermined ratio, melted in a high frequency furnace and rapidly cooled, synthesized aluminosilicate calcium glass, pulverized, and brain specific surface area value 6,000cm 2 / g It was.
On the other hand, a medium consisting of 250 g of hardwood sawdust, 250 g of coniferous sawdust, 500 g of rice bran, and 140 ml of water was prepared, and after mixing 1,000 parts of the medium with the inorganic salt and calcium aluminosilicate glass shown in Table 1, they were mixed and plastic Packed into a 850 ml wide-mouth bottle.
Thereafter, after sterilization by heating at 120 ° C. and saturated vapor pressure, the fungus was cultivated under the conditions of darkness, temperature of 25 ° C. and humidity of 55% over 2 months.
After culturing the fungus, a hole with a diameter of about 1cm is made in the center of the wide-mouthed jar (bacterial mushroom), fully absorbed, and then cultured for 4 days under conditions of 15 ° C, 95% humidity, and 20 lux illumination. The primordial primordium was formed, the illuminance was increased to 200 lux, and the culture was continued for about 2 weeks, the yield of fruiting bodies was measured, and the control contrast and quality were evaluated.
In addition, the case where only inorganic salt was used, the case where only calcium aluminosilicate glass was used, and the case where neither was used was made into the comparative example. The results are also shown in Table 1.
＜使用材料＞
無機塩イ ：無水硫酸アルミニウム、試薬一級、ブレーン比表面積値9,000cm2/g
無機塩ロ ：硫酸第一鉄、試薬一級、ブレーン比表面積値9,000cm2/g
無機塩ハ ：塩化アルミニウム、試薬一級、ブレーン比表面積値9,000cm2/g
無機塩ニ ：フッ化アルミニウム、試薬一級、ブレーン比表面積値9,000cm2/g
無機塩ホ ：リン酸水素アルミニウム、試薬一級、ブレーン比表面積値9,000cm2/g
無機塩ヘ ：硫酸ナトリウム、試薬一級、ブレーン比表面積値9,000cm2/g
無機塩ト ：炭酸水素ナトリウム、試薬一級、ブレーン比表面積値9,000cm2/g
CaO原料 ：炭酸カルシウム、関東化学社製、試薬一級
Al2O3原料 ：酸化アルミニウム、関東化学社製、試薬一級
SiO2原料 ：二酸化ケイ素、関東化学社製、試薬一級
アルミノケイ酸カルシウムガラス：CaO50.0％、Al2O3 20.0％、SiO2 30.0％、ガラス化率100％、ブレーン比表面積値6,000cm2/g
広葉樹鋸屑：ぶな材の鋸屑
針葉樹鋸屑：すぎ材の鋸屑
米糠 ：市販品
水 ：純水
<Materials used>
Inorganic salt a: Anhydrous aluminum sulfate, reagent first grade, Blaine specific surface area 9,000cm 2 / g
Inorganic salt: Ferrous sulfate, first grade reagent, Blaine specific surface area 9,000cm 2 / g
Inorganic salt ： Aluminum chloride, first grade reagent, Blaine specific surface area 9,000cm 2 / g
Inorganic salt: Aluminum fluoride, first grade reagent, Blaine specific surface area 9,000cm 2 / g
Inorganic salt ： Aluminum hydrogen phosphate, first grade reagent, Blaine specific surface area 9,000 cm 2 / g
Inorganic salt: Sodium sulfate, reagent grade 1, Brain specific surface area 9,000cm 2 / g
Inorganic salt: Sodium bicarbonate, first grade reagent, Blaine specific surface area 9,000cm 2 / g
CaO raw material: Calcium carbonate, manufactured by Kanto Chemical Co., reagent grade
Al 2 O 3 raw material: Aluminum oxide, manufactured by Kanto Chemical Co., Ltd., reagent first grade
SiO 2 raw material: Silicon dioxide, manufactured by Kanto Chemical Co., Inc., reagent grade 1 calcium aluminosilicate glass: CaO 50.0%, Al 2 O 3 20.0%, SiO 2 30.0%, vitrification rate 100%, Blaine specific surface area value 6,000cm 2 / g
Hardwood sawdust: Sawdust of beech coniferous sawdust: Sawdust of over-grown rice cake: Commercial water: Pure water
＜測定方法＞
到達時間 ：0.1規定の塩酸50mlをビーカーにとり、１ｇのきのこの栽培用添加材を加えて撹拌し、ｐＨを測定して最初のｐＨとする。撹拌開始から10分ごとに１規定の塩酸を２ml加え、最初のｐＨになるまで続けた時に、きのこの栽培用添加材を加えてからｐＨが3.0に到達するまでの時間
最高ｐＨ ：到達時間以降、ｐＨが3.0になるまでの間の最高ｐＨ
コントロール対比：収率、きのこの栽培用添加材を添加した場合の子実体収量(ｇ)／無添加の子実体収量(ｇ)×100(％)
品質 ：次の基準に基づいて目視評価した。子実体の大きさが揃っていて、傘の開きがない場合は優、子実体の大きさが揃っていない、もしくは傘がやや開き気味の場合は可、子実体の大きさが揃っておらず、傘もやや開き気味の場合は不可とした。
<Measurement method>
Time to reach: Take 50 ml of 0.1 N hydrochloric acid in a beaker, add 1 g of mushroom cultivating additive, stir and measure the pH to the initial pH. 2 ml of 1N hydrochloric acid is added every 10 minutes from the start of stirring, and when it is continued until the initial pH is reached, the time until the pH reaches 3.0 after adding the additive for growing mushrooms is the maximum pH: after the arrival time , Maximum pH between pH 3.0
Control contrast: Yield, fruit body yield (g) / additive fruit body yield (g) x 100 (%)
Quality: Visual evaluation was made based on the following criteria. If the size of the fruiting body is uniform and the umbrella does not open, it is excellent, the size of the fruiting body is not aligned, or if the umbrella is slightly open, yes, the size of the fruiting body is not aligned If the umbrella is slightly open, it was not allowed.
表１に示すように、本無機塩と、アルミノケイ酸カルシウムガラスを併用し、Fuchs法試験で測定した最高ｐＨが3.5〜4.5の範囲にあるときに、ぶなしめじの品質や収率が向上することが分かる。 As shown in Table 1, the quality and yield of bamboo shoots are improved when the present inorganic salt is used in combination with calcium aluminosilicate glass and the maximum pH measured in the Fuchs method test is in the range of 3.5 to 4.5. I understand.
実験例２
表２に示すように、無機塩とアルミノケイ酸カルシウムガラスを使用して到達時間を変化させたこと以外は、実験例１と同様に行った。結果を表２に併記する。
Experimental example 2
As shown in Table 2, the experiment was performed in the same manner as in Experimental Example 1 except that the arrival time was changed using an inorganic salt and calcium aluminosilicate glass. The results are also shown in Table 2.
表２に示すように、到達時間が120秒以内であるときに、ぶなしめじの品質や収率が向上することが分かる。 As shown in Table 2, it can be seen that when the arrival time is within 120 seconds, the quality and yield of the bamboo shoot are improved.
実験例３
アルミノケイ酸カルシウムガラスの代わりにアルカリ土類金属の複合水酸化物を使用したこと以外は、実験例１と同様に行った。結果を表３に併記する。
Experimental example 3
The experiment was performed in the same manner as in Experimental Example 1 except that an alkaline earth metal composite hydroxide was used instead of the calcium aluminosilicate glass. The results are also shown in Table 3.
＜使用材料＞
複合水酸化物：MgO-Al2O3-SiO2-H2O系化合物、MgO17.9％、Al2O3 26.4％、SiO2 23.0％、H2O 32.7％、ＢＥＴ比表面積53m2/g
<Materials used>
Compound hydroxide: MgO-Al 2 O 3 —SiO 2 —H 2 O compound, MgO 17.9%, Al 2 O 3 26.4%, SiO 2 23.0%, H 2 O 32.7%, BET specific surface area 53 m 2 / g
表３に示すように、本無機塩と、アルカリ土類金属の複合水酸化物を併用し、Fuchs法試験で測定した最高ｐＨが3.5〜4.5の範囲にある時に、ぶなしめじの品質や収率が向上することが分かる。 As shown in Table 3, the quality and yield of bamboo shoots when this inorganic salt is used in combination with an alkaline earth metal complex hydroxide and the maximum pH measured by the Fuchs test is in the range of 3.5 to 4.5. Can be seen to improve.
実験例４
無機塩イと、アルミノケイ酸カルシウムガラス、アルカリ土類金属の酸化物、アルカリ土類金属の複合酸化物、アルカリ土類金属の水酸化物、アルカリ土類金属の複合水酸化物、及びハイドロタルサイトを表４に示す割合で併用したこと以外は実験例１と同様に行った。結果を表４に併記する。
Experimental Example 4
Inorganic salt and calcium aluminosilicate glass, alkaline earth metal oxide, alkaline earth metal composite oxide, alkaline earth metal hydroxide, alkaline earth metal composite hydroxide, and hydrotalcite Was carried out in the same manner as in Experimental Example 1 except that these were used together in the ratio shown in Table 4. The results are also shown in Table 4.
＜使用材料＞
酸化物 ：酸化カルシウム、試薬一級、ブレーン比表面積値5,000cm2/g
複合酸化物：ジカルシウムシリケート、CaO 65％、SiO2 35％、ブレーン比表面積値6,000cm2/g
水酸化物 ：水酸化カルシウム、試薬一級、ブレーン比表面積値5,000cm2/g
ハイドロタルサイト：Mg4Al2(OH)12CO3・3H2O、MgO 34.4％、Al2O3 21.7％、CO2 9.4％、H2O 34.5％、ＢＥＴ比表面積値7.6m2/g
<Materials used>
Oxide: Calcium oxide, first grade reagent, Blaine specific surface area value 5,000cm 2 / g
Complex oxide: Dicalcium silicate, CaO 65%, SiO 2 35%, Blaine specific surface area value 6,000cm 2 / g
Hydroxide: Calcium hydroxide, reagent first grade, Blaine specific surface area value 5,000cm 2 / g
Hydrotalcite: Mg 4 Al 2 (OH) 12 CO 3 / 3H 2 O, MgO 34.4%, Al 2 O 3 21.7%, CO 2 9.4%, H 2 O 34.5%, BET specific surface area 7.6m 2 / g
表４に示すように、本無機塩と、アルミノケイ酸カルシウムガラス、アルカリ土類金属の酸化物、アルカリ土類金属の複合酸化物、アルカリ土類金属の水酸化物、アルカリ土類金属の複合水酸化物、及びハイドロタルサイトからなる群から選ばれた一種又は二種以上を併用すると、さらに、ぶなしめじの品質や収率が向上することがわかる。 As shown in Table 4, the present inorganic salt, calcium aluminosilicate glass, alkaline earth metal oxide, alkaline earth metal composite oxide, alkaline earth metal hydroxide, alkaline earth metal composite water It can be seen that when one or two or more selected from the group consisting of oxides and hydrotalcites are used in combination, the quality and yield of bamboo shoots are further improved.
実験例５
表５に示す割合で、本無機塩と、アルミノケイ酸カルシウムガラス、アルカリ土類金属の酸化物、アルカリ土類金属の複合酸化物、アルカリ土類金属の水酸化物、アルカリ土類金属の複合水酸化物、及びハイドロタルサイトを併用したこと以外は実験例４と同様に行った。結果を表５に示す。
Experimental Example 5
In proportions shown in Table 5, the present inorganic salt, calcium aluminosilicate glass, alkaline earth metal oxide, alkaline earth metal composite oxide, alkaline earth metal hydroxide, alkaline earth metal composite water It carried out similarly to Experimental example 4 except having used together the oxide and the hydrotalcite. The results are shown in Table 5.
表５に示すように、本無機塩と、アルミノケイ酸カルシウムガラスと、アルカリ土類金属の酸化物、アルカリ土類金属の複合酸化物、アルカリ土類金属の水酸化物、アルカリ土類金属の複合水酸化物、及びハイドロタルサイトからなる群から選ばれた二種を併用すると、さらに、ぶなしめじの品質や収率が向上することがわかる。 As shown in Table 5, this inorganic salt, calcium aluminosilicate glass, alkaline earth metal oxide, alkaline earth metal composite oxide, alkaline earth metal hydroxide, alkaline earth metal composite It can be seen that when two kinds selected from the group consisting of hydroxide and hydrotalcite are used in combination, the quality and yield of the bamboo shoots are further improved.
実験例６
表６に示す割合で、本無機塩と、アルミノケイ酸カルシウムガラス、アルカリ土類金属の酸化物、アルカリ土類金属の複合酸化物、アルカリ土類金属の水酸化物、アルカリ土類金属の複合水酸化物、及びハイドロタルサイトを併用したこと以外は実験例４と同様に行った。結果を表６に示す。
Experimental Example 6
In proportions shown in Table 6, the present inorganic salt, calcium aluminosilicate glass, alkaline earth metal oxide, alkaline earth metal composite oxide, alkaline earth metal hydroxide, alkaline earth metal composite water It carried out similarly to Experimental example 4 except having used together the oxide and the hydrotalcite. The results are shown in Table 6.
表６に示すように、本無機塩と、アルミノケイ酸カルシウムガラスと、アルカリ土類金属の酸化物、アルカリ土類金属の複合酸化物、アルカリ土類金属の水酸化物、アルカリ土類金属の複合水酸化物、及びハイドロタルサイトからなる群から選ばれた三種以上を併用すると、さらに、ぶなしめじの品質や収率が向上することがわかる。 As shown in Table 6, the present inorganic salt, calcium aluminosilicate glass, alkaline earth metal oxide, alkaline earth metal composite oxide, alkaline earth metal hydroxide, alkaline earth metal composite It can be seen that when three or more kinds selected from the group consisting of hydroxide and hydrotalcite are used in combination, the quality and yield of bamboo shoots are further improved.
本発明のきのこの人工培養基を使用すると、形や大きさが良好なきのこを高い収率で栽培できるため、えのきだけ、ひらたけ、なめこ、ぶなしめじ、まいたけ、きくらげ、さるのこしかけ、及びしいたけなど広範なきのこの栽培に使用することができる。 Since the mushroom artificial culture medium of the present invention can be used to grow mushrooms of good shape and size with a high yield, it can be used in a wide variety of fields such as enoki mushrooms, octopus mushrooms, nameko mushrooms, bean mushrooms, maitake mushrooms, jellyfish mushrooms, shiitake mushrooms, and shiitake mushrooms. Can be used for cultivation of mushrooms.
Claims (6)
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014018097A (en) * | 2012-07-13 | 2014-02-03 | Denki Kagaku Kogyo Kk | Additive for cultivation of mushroom, artificial culture medium for mushroom and artificial method for cultivating mushroom using the same |
| CN104285533A (en) * | 2014-10-22 | 2015-01-21 | 赵俊瑞 | Method for using agricultural straw to improve saline-alkali soil |
| JP2020079185A (en) * | 2018-11-14 | 2020-05-28 | デンカ株式会社 | Expansion composition, cement composition, and cement concrete |
| CN114258264A (en) * | 2019-08-21 | 2022-03-29 | 协和化学工业株式会社 | Additive for mushroom culture medium |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106332658A (en) * | 2016-08-31 | 2017-01-18 | 赵俊瑞 | Method for developing organic agriculture by straws and tillage land in idle period |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11243773A (en) * | 1998-02-27 | 1999-09-14 | Denki Kagaku Kogyo Kk | Artificial culture medium for mushroom and artificial culture of mushroom with the same |
| JP3534295B2 (en) * | 1997-12-26 | 2004-06-07 | 電気化学工業株式会社 | Mushroom artificial culture medium and mushroom artificial cultivation method using the same |
| JP2004313093A (en) * | 2003-04-17 | 2004-11-11 | Kyowa Chem Ind Co Ltd | Additive for culture medium for mushroom, and culture medium for mushroom |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP3534295B2 (en) * | 1997-12-26 | 2004-06-07 | 電気化学工業株式会社 | Mushroom artificial culture medium and mushroom artificial cultivation method using the same |
| JPH11243773A (en) * | 1998-02-27 | 1999-09-14 | Denki Kagaku Kogyo Kk | Artificial culture medium for mushroom and artificial culture of mushroom with the same |
| JP2004313093A (en) * | 2003-04-17 | 2004-11-11 | Kyowa Chem Ind Co Ltd | Additive for culture medium for mushroom, and culture medium for mushroom |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014018097A (en) * | 2012-07-13 | 2014-02-03 | Denki Kagaku Kogyo Kk | Additive for cultivation of mushroom, artificial culture medium for mushroom and artificial method for cultivating mushroom using the same |
| CN104285533A (en) * | 2014-10-22 | 2015-01-21 | 赵俊瑞 | Method for using agricultural straw to improve saline-alkali soil |
| CN104285533B (en) * | 2014-10-22 | 2016-08-24 | 赵俊瑞 | A kind of method utilizing agricultural stalk improvement alkaline land soil |
| JP2020079185A (en) * | 2018-11-14 | 2020-05-28 | デンカ株式会社 | Expansion composition, cement composition, and cement concrete |
| JP7260998B2 (en) | 2018-11-14 | 2023-04-19 | デンカ株式会社 | Expansive composition, cement composition and cement-concrete |
| CN114258264A (en) * | 2019-08-21 | 2022-03-29 | 协和化学工业株式会社 | Additive for mushroom culture medium |
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| JP5527803B2 (en) | 2014-06-25 |
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