JP2010536366A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2010536366A5 JP2010536366A5 JP2010521581A JP2010521581A JP2010536366A5 JP 2010536366 A5 JP2010536366 A5 JP 2010536366A5 JP 2010521581 A JP2010521581 A JP 2010521581A JP 2010521581 A JP2010521581 A JP 2010521581A JP 2010536366 A5 JP2010536366 A5 JP 2010536366A5
- Authority
- JP
- Japan
- Prior art keywords
- ebi3
- level
- lung cancer
- nptx1
- expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 208000020816 lung neoplasm Diseases 0.000 claims description 162
- 206010058467 Lung neoplasm malignant Diseases 0.000 claims description 150
- 201000005202 lung cancer Diseases 0.000 claims description 150
- 230000014509 gene expression Effects 0.000 claims description 129
- 101000852964 Homo sapiens Interleukin-27 subunit beta Proteins 0.000 claims description 105
- 102100036712 Interleukin-27 subunit beta Human genes 0.000 claims description 105
- 208000002154 non-small cell lung carcinoma Diseases 0.000 claims description 81
- 238000000034 method Methods 0.000 claims description 73
- 108090000623 proteins and genes Proteins 0.000 claims description 59
- 239000000523 sample Substances 0.000 claims description 45
- 210000004369 blood Anatomy 0.000 claims description 43
- 239000008280 blood Substances 0.000 claims description 43
- 210000002966 serum Anatomy 0.000 claims description 42
- 206010041067 Small cell lung cancer Diseases 0.000 claims description 33
- 102000004169 proteins and genes Human genes 0.000 claims description 31
- 230000027455 binding Effects 0.000 claims description 23
- 239000003153 chemical reaction reagent Substances 0.000 claims description 18
- 238000002965 ELISA Methods 0.000 claims description 13
- 230000004071 biological effect Effects 0.000 claims description 9
- 238000003018 immunoassay Methods 0.000 claims description 9
- 108020004999 messenger RNA Proteins 0.000 claims description 8
- 239000013641 positive control Substances 0.000 claims description 7
- 239000012472 biological sample Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 208000002151 Pleural effusion Diseases 0.000 claims description 3
- 206010036790 Productive cough Diseases 0.000 claims description 3
- 238000001574 biopsy Methods 0.000 claims description 3
- 210000002381 plasma Anatomy 0.000 claims description 3
- 210000003802 sputum Anatomy 0.000 claims description 3
- 208000024794 sputum Diseases 0.000 claims description 3
- 210000002700 urine Anatomy 0.000 claims description 3
- 239000000439 tumor marker Substances 0.000 claims 4
- 210000004027 cell Anatomy 0.000 description 204
- 101000604058 Homo sapiens Neuronal pentraxin-1 Proteins 0.000 description 156
- 102100038436 Neuronal pentraxin-1 Human genes 0.000 description 156
- 102000010292 Peptide Elongation Factor 1 Human genes 0.000 description 124
- 108010077524 Peptide Elongation Factor 1 Proteins 0.000 description 124
- 102100034770 Cyclin-dependent kinase inhibitor 3 Human genes 0.000 description 123
- 101710157591 Cyclin-dependent kinase inhibitor 3 Proteins 0.000 description 121
- 210000001519 tissue Anatomy 0.000 description 91
- 206010028980 Neoplasm Diseases 0.000 description 84
- 108090000765 processed proteins & peptides Proteins 0.000 description 54
- 102000004196 processed proteins & peptides Human genes 0.000 description 44
- 108020004459 Small interfering RNA Proteins 0.000 description 41
- 229920001184 polypeptide Polymers 0.000 description 40
- 101001108242 Homo sapiens Neuronal pentraxin receptor Proteins 0.000 description 38
- 102100021877 Neuronal pentraxin receptor Human genes 0.000 description 38
- 239000000203 mixture Substances 0.000 description 36
- 239000004055 small Interfering RNA Substances 0.000 description 36
- 230000000694 effects Effects 0.000 description 33
- 201000011510 cancer Diseases 0.000 description 31
- 208000000587 small cell lung carcinoma Diseases 0.000 description 31
- 208000009956 adenocarcinoma Diseases 0.000 description 29
- 210000004072 lung Anatomy 0.000 description 29
- 206010041823 squamous cell carcinoma Diseases 0.000 description 28
- 150000001875 compounds Chemical class 0.000 description 24
- 230000010261 cell growth Effects 0.000 description 22
- 235000018102 proteins Nutrition 0.000 description 21
- 238000004458 analytical method Methods 0.000 description 18
- 230000012010 growth Effects 0.000 description 18
- 208000006545 Chronic Obstructive Pulmonary Disease Diseases 0.000 description 17
- 238000003757 reverse transcription PCR Methods 0.000 description 17
- 230000004083 survival effect Effects 0.000 description 17
- 238000000134 MTT assay Methods 0.000 description 16
- 231100000002 MTT assay Toxicity 0.000 description 16
- 230000000875 corresponding effect Effects 0.000 description 16
- 238000010186 staining Methods 0.000 description 16
- -1 DLX5 Proteins 0.000 description 15
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 14
- 238000003556 assay Methods 0.000 description 14
- 238000002474 experimental method Methods 0.000 description 14
- 230000005764 inhibitory process Effects 0.000 description 13
- 208000037841 lung tumor Diseases 0.000 description 13
- 238000002493 microarray Methods 0.000 description 13
- 239000002773 nucleotide Substances 0.000 description 13
- 239000013598 vector Substances 0.000 description 13
- 238000001262 western blot Methods 0.000 description 13
- 108060001084 Luciferase Proteins 0.000 description 12
- 239000005089 Luciferase Substances 0.000 description 12
- 238000013211 curve analysis Methods 0.000 description 12
- 125000003729 nucleotide group Chemical group 0.000 description 12
- 238000001356 surgical procedure Methods 0.000 description 12
- 101000787278 Arabidopsis thaliana Valine-tRNA ligase, chloroplastic/mitochondrial 2 Proteins 0.000 description 11
- 101000787296 Arabidopsis thaliana Valine-tRNA ligase, mitochondrial 1 Proteins 0.000 description 11
- 101000787280 Dictyostelium discoideum Probable valine-tRNA ligase, mitochondrial Proteins 0.000 description 11
- 150000001413 amino acids Chemical group 0.000 description 11
- 239000000284 extract Substances 0.000 description 11
- 238000001114 immunoprecipitation Methods 0.000 description 11
- 230000003993 interaction Effects 0.000 description 11
- 230000002018 overexpression Effects 0.000 description 11
- 108091081021 Sense strand Proteins 0.000 description 10
- 102100025607 Valine-tRNA ligase Human genes 0.000 description 10
- 230000000692 anti-sense effect Effects 0.000 description 10
- 230000003833 cell viability Effects 0.000 description 10
- 239000003636 conditioned culture medium Substances 0.000 description 10
- 239000013612 plasmid Substances 0.000 description 10
- 238000010837 poor prognosis Methods 0.000 description 10
- 238000012360 testing method Methods 0.000 description 10
- 238000010293 colony formation assay Methods 0.000 description 9
- 239000002609 medium Substances 0.000 description 9
- 238000000729 Fisher's exact test Methods 0.000 description 8
- 241000699666 Mus <mouse, genus> Species 0.000 description 8
- 239000013604 expression vector Substances 0.000 description 8
- 230000002401 inhibitory effect Effects 0.000 description 8
- 238000012216 screening Methods 0.000 description 8
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 7
- 208000019693 Lung disease Diseases 0.000 description 7
- 238000010240 RT-PCR analysis Methods 0.000 description 7
- 230000003305 autocrine Effects 0.000 description 7
- 239000002299 complementary DNA Substances 0.000 description 7
- 210000000805 cytoplasm Anatomy 0.000 description 7
- 230000030609 dephosphorylation Effects 0.000 description 7
- 238000006209 dephosphorylation reaction Methods 0.000 description 7
- 238000001325 log-rank test Methods 0.000 description 7
- 108010082117 matrigel Proteins 0.000 description 7
- 230000004044 response Effects 0.000 description 7
- 206010068873 Adenosquamous cell carcinoma Diseases 0.000 description 6
- 108010051109 Cell-Penetrating Peptides Proteins 0.000 description 6
- 102000020313 Cell-Penetrating Peptides Human genes 0.000 description 6
- 238000000636 Northern blotting Methods 0.000 description 6
- 201000008395 adenosquamous carcinoma Diseases 0.000 description 6
- 235000001014 amino acid Nutrition 0.000 description 6
- 230000004709 cell invasion Effects 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- 238000011156 evaluation Methods 0.000 description 6
- 239000012894 fetal calf serum Substances 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 238000001727 in vivo Methods 0.000 description 6
- 238000006366 phosphorylation reaction Methods 0.000 description 6
- 108091033319 polynucleotide Proteins 0.000 description 6
- 102000040430 polynucleotide Human genes 0.000 description 6
- 239000002157 polynucleotide Substances 0.000 description 6
- 230000002829 reductive effect Effects 0.000 description 6
- 230000035945 sensitivity Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000010824 Kaplan-Meier survival analysis Methods 0.000 description 5
- 101150073764 NPTXR gene Proteins 0.000 description 5
- 230000000295 complement effect Effects 0.000 description 5
- 238000004624 confocal microscopy Methods 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 210000003743 erythrocyte Anatomy 0.000 description 5
- 210000002216 heart Anatomy 0.000 description 5
- 230000002055 immunohistochemical effect Effects 0.000 description 5
- 238000011532 immunohistochemical staining Methods 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 210000003734 kidney Anatomy 0.000 description 5
- 210000004185 liver Anatomy 0.000 description 5
- 230000026731 phosphorylation Effects 0.000 description 5
- 210000002826 placenta Anatomy 0.000 description 5
- 238000004393 prognosis Methods 0.000 description 5
- 102100022900 Actin, cytoplasmic 1 Human genes 0.000 description 4
- 108010085238 Actins Proteins 0.000 description 4
- 208000010507 Adenocarcinoma of Lung Diseases 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 4
- 206010027476 Metastases Diseases 0.000 description 4
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 4
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 4
- 230000004663 cell proliferation Effects 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 108010048367 enhanced green fluorescent protein Proteins 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 238000003119 immunoblot Methods 0.000 description 4
- 238000002991 immunohistochemical analysis Methods 0.000 description 4
- 208000003849 large cell carcinoma Diseases 0.000 description 4
- 201000005249 lung adenocarcinoma Diseases 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 230000009401 metastasis Effects 0.000 description 4
- 230000017066 negative regulation of growth Effects 0.000 description 4
- 230000001613 neoplastic effect Effects 0.000 description 4
- 230000002093 peripheral effect Effects 0.000 description 4
- 229920000724 poly(L-arginine) polymer Polymers 0.000 description 4
- 108010011110 polyarginine Proteins 0.000 description 4
- 229920000915 polyvinyl chloride Polymers 0.000 description 4
- 238000002271 resection Methods 0.000 description 4
- 230000014616 translation Effects 0.000 description 4
- AZKSAVLVSZKNRD-UHFFFAOYSA-M 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Chemical compound [Br-].S1C(C)=C(C)N=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=CC=C1 AZKSAVLVSZKNRD-UHFFFAOYSA-M 0.000 description 3
- 102000009728 CDC2 Protein Kinase Human genes 0.000 description 3
- 108010034798 CDC2 Protein Kinase Proteins 0.000 description 3
- 108020004705 Codon Proteins 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 3
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 3
- 238000000585 Mann–Whitney U test Methods 0.000 description 3
- 238000007807 Matrigel invasion assay Methods 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 102000003923 Protein Kinase C Human genes 0.000 description 3
- 108090000315 Protein Kinase C Proteins 0.000 description 3
- 108700008625 Reporter Genes Proteins 0.000 description 3
- 229920004890 Triton X-100 Polymers 0.000 description 3
- 239000013504 Triton X-100 Substances 0.000 description 3
- 210000001552 airway epithelial cell Anatomy 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 230000008827 biological function Effects 0.000 description 3
- 239000000090 biomarker Substances 0.000 description 3
- 210000000601 blood cell Anatomy 0.000 description 3
- 210000004556 brain Anatomy 0.000 description 3
- 210000000170 cell membrane Anatomy 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 239000012568 clinical material Substances 0.000 description 3
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 230000002950 deficient Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 210000000981 epithelium Anatomy 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 238000007429 general method Methods 0.000 description 3
- 238000003364 immunohistochemistry Methods 0.000 description 3
- 201000005243 lung squamous cell carcinoma Diseases 0.000 description 3
- 230000002980 postoperative effect Effects 0.000 description 3
- 108010061031 pro-gastrin-releasing peptide (31-98) Proteins 0.000 description 3
- 230000000750 progressive effect Effects 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 210000001550 testis Anatomy 0.000 description 3
- 238000001890 transfection Methods 0.000 description 3
- 230000035899 viability Effects 0.000 description 3
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 2
- 208000003174 Brain Neoplasms Diseases 0.000 description 2
- 206010006187 Breast cancer Diseases 0.000 description 2
- 208000026310 Breast neoplasm Diseases 0.000 description 2
- 102000052052 Casein Kinase II Human genes 0.000 description 2
- 108010010919 Casein Kinase II Proteins 0.000 description 2
- 101150014361 Delta gene Proteins 0.000 description 2
- 101150118728 Dlx5 gene Proteins 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 108700020796 Oncogene Proteins 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 2
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 2
- 102000013625 Valine-tRNA Ligase Human genes 0.000 description 2
- 102100036976 X-ray repair cross-complementing protein 6 Human genes 0.000 description 2
- 101710124907 X-ray repair cross-complementing protein 6 Proteins 0.000 description 2
- 210000004100 adrenal gland Anatomy 0.000 description 2
- 125000000266 alpha-aminoacyl group Chemical group 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 230000030570 cellular localization Effects 0.000 description 2
- 230000008045 co-localization Effects 0.000 description 2
- 210000001072 colon Anatomy 0.000 description 2
- 230000005757 colony formation Effects 0.000 description 2
- 230000009918 complex formation Effects 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 239000000104 diagnostic biomarker Substances 0.000 description 2
- SLPJGDQJLTYWCI-UHFFFAOYSA-N dimethyl-(4,5,6,7-tetrabromo-1h-benzoimidazol-2-yl)-amine Chemical compound BrC1=C(Br)C(Br)=C2NC(N(C)C)=NC2=C1Br SLPJGDQJLTYWCI-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000013613 expression plasmid Substances 0.000 description 2
- 230000009368 gene silencing by RNA Effects 0.000 description 2
- BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Chemical compound O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 2
- 210000004408 hybridoma Anatomy 0.000 description 2
- 238000012744 immunostaining Methods 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 230000001394 metastastic effect Effects 0.000 description 2
- 206010061289 metastatic neoplasm Diseases 0.000 description 2
- 238000000491 multivariate analysis Methods 0.000 description 2
- 108010000889 neuronal pentraxin Proteins 0.000 description 2
- 230000003472 neutralizing effect Effects 0.000 description 2
- 238000011580 nude mouse model Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000001243 protein synthesis Methods 0.000 description 2
- 238000011470 radical surgery Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 229940124597 therapeutic agent Drugs 0.000 description 2
- 230000002103 transcriptional effect Effects 0.000 description 2
- 230000010474 transient expression Effects 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 238000007473 univariate analysis Methods 0.000 description 2
- 230000003827 upregulation Effects 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- FALRKNHUBBKYCC-UHFFFAOYSA-N 2-(chloromethyl)pyridine-3-carbonitrile Chemical compound ClCC1=NC=CC=C1C#N FALRKNHUBBKYCC-UHFFFAOYSA-N 0.000 description 1
- 101710151906 31 kDa protein Proteins 0.000 description 1
- 108010062307 AAVALLPAVLLALLAP Proteins 0.000 description 1
- 102000052866 Amino Acyl-tRNA Synthetases Human genes 0.000 description 1
- 108700028939 Amino Acyl-tRNA Synthetases Proteins 0.000 description 1
- 101800002011 Amphipathic peptide Proteins 0.000 description 1
- 108020005544 Antisense RNA Proteins 0.000 description 1
- 208000002109 Argyria Diseases 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 238000011729 BALB/c nude mouse Methods 0.000 description 1
- 101000798396 Bacillus licheniformis Phenylalanine racemase [ATP hydrolyzing] Proteins 0.000 description 1
- 101000609447 Beet necrotic yellow vein virus (isolate Japan/S) Protein P25 Proteins 0.000 description 1
- 101000914947 Bungarus multicinctus Long neurotoxin homolog TA-bm16 Proteins 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- 201000009030 Carcinoma Diseases 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 1
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 1
- 206010061819 Disease recurrence Diseases 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 102100023362 Elongation factor 1-gamma Human genes 0.000 description 1
- 102100028072 Fibroblast growth factor 4 Human genes 0.000 description 1
- 108091006027 G proteins Proteins 0.000 description 1
- 102000030782 GTP binding Human genes 0.000 description 1
- 108091000058 GTP-Binding Proteins 0.000 description 1
- 238000002738 Giemsa staining Methods 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 102000016285 Guanine Nucleotide Exchange Factors Human genes 0.000 description 1
- 108010067218 Guanine Nucleotide Exchange Factors Proteins 0.000 description 1
- 108010061875 HN-1 peptide Proteins 0.000 description 1
- 101001050451 Homo sapiens Elongation factor 1-gamma Proteins 0.000 description 1
- 101001060274 Homo sapiens Fibroblast growth factor 4 Proteins 0.000 description 1
- 101000901627 Homo sapiens Homeobox protein DLX-5 Proteins 0.000 description 1
- 101000787286 Homo sapiens Valine-tRNA ligase Proteins 0.000 description 1
- 108700003968 Human immunodeficiency virus 1 tat peptide (49-57) Proteins 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 239000012097 Lipofectamine 2000 Substances 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 206010059282 Metastases to central nervous system Diseases 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 1
- 108010088535 Pep-1 peptide Proteins 0.000 description 1
- 102000002508 Peptide Elongation Factors Human genes 0.000 description 1
- 108010068204 Peptide Elongation Factors Proteins 0.000 description 1
- 101000622060 Photinus pyralis Luciferin 4-monooxygenase Proteins 0.000 description 1
- 102000029797 Prion Human genes 0.000 description 1
- 108091000054 Prion Proteins 0.000 description 1
- 108010029485 Protein Isoforms Proteins 0.000 description 1
- 102000001708 Protein Isoforms Human genes 0.000 description 1
- 101710149951 Protein Tat Proteins 0.000 description 1
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 108091030071 RNAI Proteins 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 102000002278 Ribosomal Proteins Human genes 0.000 description 1
- 108010000605 Ribosomal Proteins Proteins 0.000 description 1
- 238000011579 SCID mouse model Methods 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 238000003646 Spearman's rank correlation coefficient Methods 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- 108010034949 Thyroglobulin Proteins 0.000 description 1
- 102000009843 Thyroglobulin Human genes 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 101710162629 Trypsin inhibitor Proteins 0.000 description 1
- 229940122618 Trypsin inhibitor Drugs 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 1
- 238000011888 autopsy Methods 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 230000002146 bilateral effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 201000008275 breast carcinoma Diseases 0.000 description 1
- 210000000424 bronchial epithelial cell Anatomy 0.000 description 1
- 108010025307 buforin II Proteins 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 230000005907 cancer growth Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 238000010609 cell counting kit-8 assay Methods 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 230000012292 cell migration Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- UKVZSPHYQJNTOU-IVBHRGSNSA-N chembl1240717 Chemical compound C([C@H](NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@@H](N)[C@H](C)O)CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(O)=O)C1=CC=CC=C1 UKVZSPHYQJNTOU-IVBHRGSNSA-N 0.000 description 1
- BHONFOAYRQZPKZ-LCLOTLQISA-N chembl269478 Chemical compound C([C@H](NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CCCNC(N)=N)[C@@H](C)CC)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(O)=O)C1=CC=CC=C1 BHONFOAYRQZPKZ-LCLOTLQISA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 1
- 238000012303 cytoplasmic staining Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 229950006137 dexfosfoserine Drugs 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 230000001819 effect on gene Effects 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 206010016629 fibroma Diseases 0.000 description 1
- 238000003197 gene knockdown Methods 0.000 description 1
- 230000009422 growth inhibiting effect Effects 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 230000009640 growth promoting pathway Effects 0.000 description 1
- 238000007490 hematoxylin and eosin (H&E) staining Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 102000051586 human DLX5 Human genes 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 239000012133 immunoprecipitate Substances 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 238000007912 intraperitoneal administration Methods 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 108010045069 keyhole-limpet hemocyanin Proteins 0.000 description 1
- 201000009546 lung large cell carcinoma Diseases 0.000 description 1
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 229940126619 mouse monoclonal antibody Drugs 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 231100000707 mutagenic chemical Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 230000000683 nonmetastatic effect Effects 0.000 description 1
- 230000003076 paracrine Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 108010043655 penetratin Proteins 0.000 description 1
- MCYTYTUNNNZWOK-LCLOTLQISA-N penetratin Chemical compound C([C@H](NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CCCNC(N)=N)[C@@H](C)CC)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(N)=O)C1=CC=CC=C1 MCYTYTUNNNZWOK-LCLOTLQISA-N 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 201000008814 placenta cancer Diseases 0.000 description 1
- 230000023603 positive regulation of transcription initiation, DNA-dependent Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000003518 presynaptic effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002685 pulmonary effect Effects 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000003998 snake venom Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000004960 subcellular localization Effects 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 229940014800 succinic anhydride Drugs 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 210000000225 synapse Anatomy 0.000 description 1
- 230000000946 synaptic effect Effects 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 229960002175 thyroglobulin Drugs 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 230000002463 transducing effect Effects 0.000 description 1
- 239000012096 transfection reagent Substances 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 108010062760 transportan Proteins 0.000 description 1
- PBKWZFANFUTEPS-CWUSWOHSSA-N transportan Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(N)=O)[C@@H](C)CC)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)CN)[C@@H](C)O)C1=CC=C(O)C=C1 PBKWZFANFUTEPS-CWUSWOHSSA-N 0.000 description 1
- 210000002993 trophoblast Anatomy 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 239000002753 trypsin inhibitor Substances 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 231100000588 tumorigenic Toxicity 0.000 description 1
- 230000000381 tumorigenic effect Effects 0.000 description 1
- 230000002100 tumorsuppressive effect Effects 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US95795607P | 2007-08-24 | 2007-08-24 | |
| US97736007P | 2007-10-03 | 2007-10-03 | |
| PCT/JP2008/065352 WO2009028580A1 (en) | 2007-08-24 | 2008-08-21 | Ebi3, dlx5, nptx1 and cdkn3 for target genes of lung cancer therapy and diagnosis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2010536366A JP2010536366A (ja) | 2010-12-02 |
| JP2010536366A5 true JP2010536366A5 (https=) | 2012-10-18 |
Family
ID=40387295
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010521581A Pending JP2010536366A (ja) | 2007-08-24 | 2008-08-21 | 肺癌の治療及び診断の標的遺伝子のためのｅｂｉ３、ｄｌｘ５、ｎｐｔｘ１、及びｃｄｋｎ３ |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20110152345A1 (https=) |
| EP (1) | EP2198021A4 (https=) |
| JP (1) | JP2010536366A (https=) |
| KR (1) | KR20100075857A (https=) |
| CN (1) | CN101835894A (https=) |
| BR (1) | BRPI0815769A2 (https=) |
| CA (1) | CA2697513A1 (https=) |
| RU (1) | RU2010111120A (https=) |
| TW (1) | TW200920406A (https=) |
| WO (1) | WO2009028580A1 (https=) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW200413725A (en) * | 2002-09-30 | 2004-08-01 | Oncotherapy Science Inc | Method for diagnosing non-small cell lung cancers |
| CN101283106A (zh) * | 2005-07-27 | 2008-10-08 | 肿瘤疗法科学股份有限公司 | 小细胞肺癌的诊断方法 |
| WO2011145085A2 (en) * | 2010-05-21 | 2011-11-24 | Procognia (Israel) Ltd | Novel antibodies and methods of use for the treatment and diagnosis of cancer |
| WO2011150974A1 (en) * | 2010-06-03 | 2011-12-08 | Porto Conte Ricerche S.R.L. | Biomarkers for lung neuroendocrine tumors |
| WO2012054732A2 (en) * | 2010-10-20 | 2012-04-26 | Rush University Medical Center | Lung cancer tests |
| US20120295803A1 (en) * | 2011-05-16 | 2012-11-22 | The Regents Of The University Of Michigan | Lung cancer signature |
| US20140220006A1 (en) * | 2013-02-01 | 2014-08-07 | Meso Scale Technologies, Llc | Lung cancer biomarkers |
| US9753037B2 (en) | 2013-03-15 | 2017-09-05 | Rush University Medical Center | Biomarker panel for detecting lung cancer |
| US20150038365A1 (en) * | 2013-08-01 | 2015-02-05 | Meso Scale Technologies, Llc | Lung cancer biomarkers |
| US10365281B2 (en) | 2013-12-09 | 2019-07-30 | Rush University Medical Center | Biomarkers of rapid progression in advanced non-small cell lung cancer |
| KR101601943B1 (ko) | 2014-02-28 | 2016-03-09 | 이화여자대학교 산학협력단 | 난소암 환자의 항암제 치료 반응성 예측을 위한 마커 uchl1 |
| KR101583673B1 (ko) | 2014-02-28 | 2016-01-08 | 이화여자대학교 산학협력단 | 난소암 환자의 항암제 치료 반응성 예측을 위한 마커 dlx5 |
| KR101601941B1 (ko) | 2014-02-28 | 2016-03-09 | 이화여자대학교 산학협력단 | 난소암 환자의 항암제 치료 반응성 예측을 위한 마커 mta3 |
| KR101601942B1 (ko) | 2014-02-28 | 2016-03-09 | 이화여자대학교 산학협력단 | 난소암 환자의 항암제 치료 반응성 예측을 위한 마커 tet1 |
| CN106018807A (zh) * | 2016-05-11 | 2016-10-12 | 卢氏实验室公司 | 一种快速诊断和监控肺癌的免疫层析检测条及其制备方法 |
| CN108182346B (zh) * | 2016-12-08 | 2021-07-30 | 杭州康万达医药科技有限公司 | 预测siRNA针对某类细胞的毒性的机器学习模型的建立方法及其应用 |
| US11619634B2 (en) | 2017-08-21 | 2023-04-04 | Savicell Diagnostic Ltd. | Methods of diagnosing and treating lung cancer |
| JP7312451B2 (ja) * | 2018-01-26 | 2023-07-21 | 国立大学法人東海国立大学機構 | 受容体タンパク質を標的とする治療薬、検査薬、受容体タンパク質に結合する抗体、及び分子標的薬のスクリーニング方法 |
| SG10201802979VA (en) * | 2018-04-10 | 2019-11-28 | Kah Meng Lim | Immunological extract and method of production |
| CN110938129B (zh) * | 2019-11-08 | 2021-07-13 | 上海交通大学 | 一种生物活性多肽sklvpvgygirkl及其制备方法和应用 |
| CN111116744A (zh) * | 2020-01-07 | 2020-05-08 | 长江大学 | 哺乳动物eEF1Bα蛋白磷酸化Ser106位点的特异性抗体的制备方法及其应用 |
| WO2024213695A1 (en) * | 2023-04-14 | 2024-10-17 | Technische Universitaet Muenchen | Method for producing the subunits of human interleukin 35 from interleukin 12 and interleukin 27 |
| EP4635983A1 (en) * | 2024-04-15 | 2025-10-22 | Ymmunobio AG | A novel antibody binding specifically to nptxr and use thereof |
Family Cites Families (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3773919A (en) | 1969-10-23 | 1973-11-20 | Du Pont | Polylactide-drug mixtures |
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| US5260203A (en) | 1986-09-02 | 1993-11-09 | Enzon, Inc. | Single polypeptide chain binding molecules |
| US5567610A (en) | 1986-09-04 | 1996-10-22 | Bioinvent International Ab | Method of producing human monoclonal antibodies and kit therefor |
| GB8823869D0 (en) | 1988-10-12 | 1988-11-16 | Medical Res Council | Production of antibodies |
| US5175384A (en) | 1988-12-05 | 1992-12-29 | Genpharm International | Transgenic mice depleted in mature t-cells and methods for making transgenic mice |
| US5229275A (en) | 1990-04-26 | 1993-07-20 | Akzo N.V. | In-vitro method for producing antigen-specific human monoclonal antibodies |
| US5571894A (en) | 1991-02-05 | 1996-11-05 | Ciba-Geigy Corporation | Recombinant antibodies specific for a growth factor receptor |
| ES2136092T3 (es) | 1991-09-23 | 1999-11-16 | Medical Res Council | Procedimientos para la produccion de anticuerpos humanizados. |
| FI941572L (fi) | 1991-10-07 | 1994-05-27 | Oncologix Inc | Anti-erbB-2-monoklonaalisten vasta-aineiden yhdistelmä ja käyttömenetelmä |
| AU675929B2 (en) | 1992-02-06 | 1997-02-27 | Curis, Inc. | Biosynthetic binding protein for cancer marker |
| ATE244763T1 (de) | 1992-02-11 | 2003-07-15 | Cell Genesys Inc | Erzielen von homozygotem durch zielgerichtete genetische ereignisse |
| US5573905A (en) | 1992-03-30 | 1996-11-12 | The Scripps Research Institute | Encoded combinatorial chemical libraries |
| US5641870A (en) | 1995-04-20 | 1997-06-24 | Genentech, Inc. | Low pH hydrophobic interaction chromatography for antibody purification |
| CA2226575C (en) | 1995-07-27 | 2011-10-18 | Genentech, Inc. | Stabile isotonic lyophilized protein formulation |
| WO1997013589A1 (en) * | 1995-10-10 | 1997-04-17 | Minnesota Mining And Manufacturing Company | Corrosion protection coating system |
| US5770380A (en) | 1996-09-13 | 1998-06-23 | University Of Pittsburgh | Synthetic antibody mimics--multiple peptide loops attached to a molecular scaffold |
| US7115396B2 (en) | 1998-12-10 | 2006-10-03 | Compound Therapeutics, Inc. | Protein scaffolds for antibody mimics and other binding proteins |
| US6818418B1 (en) | 1998-12-10 | 2004-11-16 | Compound Therapeutics, Inc. | Protein scaffolds for antibody mimics and other binding proteins |
| US20050053973A1 (en) | 2001-04-26 | 2005-03-10 | Avidia Research Institute | Novel proteins with targeted binding |
| US20040175756A1 (en) | 2001-04-26 | 2004-09-09 | Avidia Research Institute | Methods for using combinatorial libraries of monomer domains |
| DK1390535T3 (da) | 2001-04-26 | 2010-12-06 | Amgen Mountain View Inc | Kombinatoriske biblioteker af monomer-domæner |
| US20050048512A1 (en) | 2001-04-26 | 2005-03-03 | Avidia Research Institute | Combinatorial libraries of monomer domains |
| US20050089932A1 (en) | 2001-04-26 | 2005-04-28 | Avidia Research Institute | Novel proteins with targeted binding |
| US20040038877A1 (en) * | 2001-10-02 | 2004-02-26 | Alsobrook John P. | Therapeutic polypeptides, nucleic acids encoding same, and methods of use |
| US20060024692A1 (en) * | 2002-09-30 | 2006-02-02 | Oncotherapy Science, Inc. | Method for diagnosing non-small cell lung cancers |
| CA2500405A1 (en) * | 2002-09-30 | 2004-04-15 | Oncotherapy Science, Inc. | Genes and polypeptides relating to human myeloid leukemia |
| WO2004094636A1 (en) * | 2003-04-24 | 2004-11-04 | Galapagos Genomics N.V. | Effective sirna knock-down constructs |
| ATE496142T1 (de) * | 2004-03-23 | 2011-02-15 | Oncotherapy Science Inc | Verfahren zur diagnose von nicht-kleinzelligem lungenkrebs |
| HRP20171010T8 (hr) | 2004-08-23 | 2017-11-03 | Sylentis S.A.U. | Liječenje poremećaja očiju koji su karakterizirani povišenim intraokularnim pritiskom pomoću sirnk |
| EP1856278A2 (en) * | 2005-02-10 | 2007-11-21 | Oncotherapy Science, Inc. | Method of diagnosing bladder cancer |
| EP2177614B1 (en) * | 2005-05-02 | 2014-12-31 | Toray Industries, Inc. | Composition and method for diagnosing esophageal cancer and metastasis of esophageal cancer |
| US7504225B2 (en) * | 2005-05-12 | 2009-03-17 | Applied Genomics, Inc. | Reagents and methods for use in cancer diagnosis, classification and therapy |
| WO2007013671A2 (en) * | 2005-07-27 | 2007-02-01 | Oncotherapy Science, Inc. | Method of diagnosing esophageal cancer |
| CN101283106A (zh) * | 2005-07-27 | 2008-10-08 | 肿瘤疗法科学股份有限公司 | 小细胞肺癌的诊断方法 |
| DE102005049800B4 (de) * | 2005-10-18 | 2009-09-17 | Johannes-Gutenberg-Universität Mainz | Verfahren zur Auffindung von Inhibitoren des Epstein-Barr-Virus-induzierten Gens 3 (EBI 3) und deren Verwendungen bei der Behandlung von metastasierenden Tumoren und allergischem Asthma |
| EP1969363B1 (en) * | 2005-12-22 | 2016-11-30 | Abbott Molecular Inc. | Methods and marker combinations for screening for predisposition to lung cancer |
| EP2061900A2 (en) * | 2006-08-25 | 2009-05-27 | Oncotherapy Science, Inc. | Prognostic markers and therapeutic targets for lung cancer |
-
2008
- 2008-08-21 RU RU2010111120/10A patent/RU2010111120A/ru unknown
- 2008-08-21 CA CA2697513A patent/CA2697513A1/en not_active Abandoned
- 2008-08-21 WO PCT/JP2008/065352 patent/WO2009028580A1/en not_active Ceased
- 2008-08-21 CN CN200880113403A patent/CN101835894A/zh active Pending
- 2008-08-21 KR KR1020107006480A patent/KR20100075857A/ko not_active Withdrawn
- 2008-08-21 EP EP08828793A patent/EP2198021A4/en not_active Withdrawn
- 2008-08-21 TW TW097131917A patent/TW200920406A/zh unknown
- 2008-08-21 BR BRPI0815769A patent/BRPI0815769A2/pt not_active IP Right Cessation
- 2008-08-21 JP JP2010521581A patent/JP2010536366A/ja active Pending
- 2008-08-21 US US12/674,759 patent/US20110152345A1/en not_active Abandoned
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2010536366A5 (https=) | ||
| JP4425141B2 (ja) | 非小細胞肺癌の診断のための方法 | |
| JP5109131B2 (ja) | 膀胱癌を診断する方法 | |
| US20110152345A1 (en) | Ebi3, dlx5, nptx1 and cdkn3 for target genes of lung cancer therapy and diagnosis | |
| JP6014904B2 (ja) | Ｅｒａｐ１由来ペプチド及びその使用 | |
| US20110263012A1 (en) | Screening and therapeutic method for nsclc targeting cdca1-kntc2 complex | |
| JP2010512730A (ja) | 肺癌の腫瘍マーカーおよび治療標的としてのｔｔｋ | |
| CN110302382A (zh) | 一种靶向肿瘤细胞的药物 | |
| AU2011292809B2 (en) | BARD1 isoforms in lung and colorectal cancer and use thereof | |
| US20120322075A1 (en) | Lung Tumor Markers and Methods of Use Thereof | |
| US20100184047A1 (en) | Screening and therapeutic method for nsclc targeting the cdca8-aurkb complex | |
| US9822184B2 (en) | DCLK1 short form specific binding agents | |
| US20110165178A1 (en) | Diagnosis of prostate cancer | |
| JP2010501162A (ja) | 肺癌の治療標的及び予後指標としてのｉｍｐ−１癌遺伝子 | |
| JP6057408B2 (ja) | 癌の予防剤および／または治療剤 | |
| JP2012501168A (ja) | 腫瘍マーカー及び癌に対する治療標的としてのｔｂｃ１ｄ７ | |
| US20120195916A1 (en) | Method of treating cancer by inhibiting trim59 expression or activity | |
| RU2813996C2 (ru) | Слитый белок из белка dctn1 с белком ret | |
| HK40072991B (zh) | Ttpal作为胃癌监测或预後判断标志物的应用 | |
| HK1227995B (en) | Bard1 isoforms in lung and colorectal cancer, detection method and use thereof |