JP2010516255A5 - - Google Patents
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- Publication number
- JP2010516255A5 JP2010516255A5 JP2009546511A JP2009546511A JP2010516255A5 JP 2010516255 A5 JP2010516255 A5 JP 2010516255A5 JP 2009546511 A JP2009546511 A JP 2009546511A JP 2009546511 A JP2009546511 A JP 2009546511A JP 2010516255 A5 JP2010516255 A5 JP 2010516255A5
- Authority
- JP
- Japan
- Prior art keywords
- pluripotent stem
- culture
- human pluripotent
- stem cells
- stem cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 210000001778 pluripotent stem cell Anatomy 0.000 claims description 30
- 239000001963 growth medium Substances 0.000 claims description 22
- 238000004113 cell culture Methods 0.000 claims description 13
- 229940079593 drug Drugs 0.000 claims description 11
- 239000003814 drug Substances 0.000 claims description 11
- 239000003795 chemical substances by application Substances 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 210000000130 stem cell Anatomy 0.000 claims description 9
- 238000010367 cloning Methods 0.000 claims description 8
- 229940043355 kinase inhibitor Drugs 0.000 claims description 4
- 239000003757 phosphotransferase inhibitor Substances 0.000 claims description 4
- 210000004027 cell Anatomy 0.000 claims description 3
- 210000002966 serum Anatomy 0.000 claims description 3
- 150000003384 small molecules Chemical class 0.000 claims description 2
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 claims 12
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 claims 12
- 238000012258 culturing Methods 0.000 claims 3
- 210000001671 embryonic stem cell Anatomy 0.000 claims 3
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims 3
- 108091000080 Phosphotransferase Proteins 0.000 claims 1
- 239000006143 cell culture medium Substances 0.000 claims 1
- 239000003112 inhibitor Substances 0.000 claims 1
- 238000012423 maintenance Methods 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 102000020233 phosphotransferase Human genes 0.000 claims 1
- 239000011435 rock Substances 0.000 claims 1
- 239000003102 growth factor Substances 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 101710175516 14 kDa zinc-binding protein Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- ZPFIEFYHXOMDRX-UHFFFAOYSA-N CCc1cncc2cccc(S(N3C(C)CNCCC3)(=O)=O)c12 Chemical compound CCc1cncc2cccc(S(N3C(C)CNCCC3)(=O)=O)c12 ZPFIEFYHXOMDRX-UHFFFAOYSA-N 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102000010780 Platelet-Derived Growth Factor Human genes 0.000 description 1
- 108010038512 Platelet-Derived Growth Factor Proteins 0.000 description 1
- 229940123924 Protein kinase C inhibitor Drugs 0.000 description 1
- 102000004338 Transferrin Human genes 0.000 description 1
- 108090000901 Transferrin Proteins 0.000 description 1
- 102000009618 Transforming Growth Factors Human genes 0.000 description 1
- 108010009583 Transforming Growth Factors Proteins 0.000 description 1
- 239000003099 cyclic gmp dependent protein kinase inhibitor Substances 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 229940043437 protein kinase A inhibitor Drugs 0.000 description 1
- 239000012656 protein kinase A inhibitor Substances 0.000 description 1
- 239000003881 protein kinase C inhibitor Substances 0.000 description 1
- 239000012581 transferrin Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US88074707P | 2007-01-17 | 2007-01-17 | |
| US60/880,747 | 2007-01-17 | ||
| PCT/US2008/051347 WO2008089351A1 (en) | 2007-01-17 | 2008-01-17 | Improved culture of stem cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010516255A JP2010516255A (ja) | 2010-05-20 |
| JP2010516255A5 true JP2010516255A5 (enExample) | 2013-09-12 |
| JP5419279B2 JP5419279B2 (ja) | 2014-02-19 |
Family
ID=39277243
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009546511A Active JP5419279B2 (ja) | 2007-01-17 | 2008-01-17 | 改良された幹細胞の培養 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7892830B2 (enExample) |
| JP (1) | JP5419279B2 (enExample) |
| CA (1) | CA2675445C (enExample) |
| GB (1) | GB2458863B (enExample) |
| SE (1) | SE0950586L (enExample) |
| WO (1) | WO2008089351A1 (enExample) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4667241B2 (ja) * | 2003-06-27 | 2011-04-06 | 旭化成株式会社 | 細胞分化抑制剤、これを用いた細胞培養方法、培養液及び培養された細胞株 |
| EP1962719A4 (en) | 2005-08-29 | 2011-05-04 | Technion Res And Dev Of Foundation Ltd | MEDIA FOR BREEDING STEM CELLS |
| US8129187B2 (en) | 2005-12-13 | 2012-03-06 | Kyoto University | Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2 |
| US20090227032A1 (en) * | 2005-12-13 | 2009-09-10 | Kyoto University | Nuclear reprogramming factor and induced pluripotent stem cells |
| KR101420740B1 (ko) * | 2005-12-13 | 2014-07-17 | 교또 다이가꾸 | 핵초기화 인자 |
| US8278104B2 (en) * | 2005-12-13 | 2012-10-02 | Kyoto University | Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2 |
| DK3441459T3 (da) | 2006-08-02 | 2021-06-07 | Technion Res & Dev Foundation | Fremgangsmåder til ekspansion af embryonale stamceller i en suspensionskultur |
| JP2008099662A (ja) | 2006-09-22 | 2008-05-01 | Institute Of Physical & Chemical Research | 幹細胞の培養方法 |
| US9213999B2 (en) * | 2007-06-15 | 2015-12-15 | Kyoto University | Providing iPSCs to a customer |
| JP2008307007A (ja) * | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞 |
| ES2690554T3 (es) | 2008-03-17 | 2018-11-21 | The Scripps Research Institute | Enfoques químicos y genéticos combinados para la generación de células madre pluripotentes inducidas |
| WO2009116840A2 (en) * | 2008-03-21 | 2009-09-24 | Industry-Academic Cooperation Foundation, Yonsei University | Chemical reagents regulating stem cell fate |
| ES2722198T3 (es) | 2008-05-02 | 2019-08-08 | Univ Kyoto | Método de reprogramación nuclear |
| WO2009135206A1 (en) | 2008-05-02 | 2009-11-05 | Stem Cell Products, Inc. | Method for production of mast cells from stem cells |
| US8648170B2 (en) * | 2008-09-19 | 2014-02-11 | Wisconsin Alumni Research Foundation | Peptide-presenting surfaces for long-term culture of pluripotent cells |
| EP3260534A1 (en) * | 2008-11-20 | 2017-12-27 | Janssen Biotech, Inc. | Pluripotent stem cell culture on micro-carriers |
| ES2645869T3 (es) | 2008-12-17 | 2017-12-11 | The Scripps Research Institute | Generación y mantenimiento de células madre |
| US8642334B2 (en) | 2009-02-17 | 2014-02-04 | Memorial Sloan Kettering Cancer Center | Methods of neural conversion of human embryonic stem cells |
| JP6189581B2 (ja) * | 2009-02-20 | 2017-08-30 | セルラー ダイナミクス インターナショナル， インコーポレイテッド | 幹細胞の分化のための方法および組成物 |
| WO2010107392A1 (en) * | 2009-03-20 | 2010-09-23 | Agency For Science, Technology And Research | Culture of pluripotent and multipotent cells on microcarriers |
| US8193235B2 (en) * | 2009-06-12 | 2012-06-05 | University Of Kansas | Compositions and methods for establishing and maintaining stem cells in an undifferentiated state |
| ES2638464T3 (es) * | 2009-10-16 | 2017-10-20 | The Scripps Research Institute | Inducción de células pluripotentes |
| CA2777552C (en) | 2009-10-19 | 2019-05-21 | Cellular Dynamics International, Inc. | Cardiomyocyte production |
| ES2539487T3 (es) * | 2009-11-04 | 2015-07-01 | Cellular Dynamics International, Inc. | Reprogramación episómica con compuestos químicos |
| CN102712896A (zh) * | 2009-11-06 | 2012-10-03 | Rnl生物技术株式会社 | 用于增殖毛囊干细胞的方法 |
| EP2499236B1 (en) | 2009-11-12 | 2020-01-01 | Technion Research & Development Foundation Ltd. | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
| US10947499B2 (en) * | 2009-12-02 | 2021-03-16 | Research Development Foundation | Selection of stem cell clones with defined differentiation capabilities |
| AU2011235212B2 (en) | 2010-03-31 | 2014-07-31 | The Scripps Research Institute | Reprogramming cells |
| EP3399026B1 (en) | 2010-06-14 | 2024-06-26 | The Scripps Research Institute | Reprogramming of cells to a new fate |
| WO2012020845A1 (ja) * | 2010-08-09 | 2012-02-16 | 武田薬品工業株式会社 | 膵ホルモン産生細胞の製造法 |
| EP3056198A2 (en) * | 2010-09-16 | 2016-08-17 | MSP Co., Ltd | Use of compounds for inducing differentiation of mesenchymal stem cells to chondrocytes |
| US9181529B2 (en) | 2010-10-19 | 2015-11-10 | Cellular Dynamics International, Inc. | Titration of differentiation medium components |
| CN106893692B (zh) | 2010-12-22 | 2021-11-26 | 菲特治疗公司 | 用于单细胞分选与增强ipsc重新编程的细胞培养平台 |
| EP2676140A2 (en) * | 2011-02-14 | 2013-12-25 | Life Technologies Corporation | Substrates and methods for staining live cells |
| US9487752B2 (en) | 2011-03-30 | 2016-11-08 | Cellular Dynamics International, Inc. | Priming of pluripotent stem cells for neural differentiation |
| JP2014520551A (ja) | 2011-07-11 | 2014-08-25 | セルラー ダイナミクス インターナショナル， インコーポレイテッド | 細胞のリプログラミング方法およびゲノムの改変方法 |
| CN110684734A (zh) | 2011-11-04 | 2020-01-14 | 纪念斯隆-凯特琳癌症中心 | 用于植入的中脑多巴胺（da）神经元 |
| WO2013082268A1 (en) | 2011-11-30 | 2013-06-06 | The Wistar Institute Of Anatomy And Biology | Methods and compositions for regulation of cell aging, carcinogenesis, and reprogramming |
| US10266807B2 (en) | 2013-04-03 | 2019-04-23 | FUJIFILM Cellular Dynamics, Inc. | Methods and compositions for culturing endoderm progenitor cells in suspension |
| EP3498824A1 (en) | 2013-04-26 | 2019-06-19 | Memorial Sloan-Kettering Cancer Center | Cortical interneurons and other neuronal cells produced by the directed differentiation of pluripotent and multipotent cells |
| SG10201807292YA (en) | 2014-03-04 | 2018-09-27 | Fate Therapeutics Inc | Improved reprogramming methods and cell culture platforms |
| ES2939807T3 (es) | 2014-03-21 | 2023-04-27 | Fujifilm Cellular Dynamics Inc | Producción de neuronas dopaminérgicas del mesencéfalo y métodos para su utilización |
| EP3183338B1 (en) | 2014-08-19 | 2020-03-04 | FUJIFILM Cellular Dynamics, Inc. | Neural networks formed from cells derived from pluripotent stem cells |
| EP3207122B1 (en) | 2014-10-14 | 2019-05-08 | FUJIFILM Cellular Dynamics, Inc. | Generation of keratinocytes from pluripotent stem cells and maintenance of keratinocyte cultures |
| AU2016338680B2 (en) | 2015-10-16 | 2022-11-17 | Fate Therapeutics, Inc. | Platform for the induction and maintenance of ground state pluripotency |
| AU2016341982B2 (en) | 2015-10-21 | 2022-07-07 | Indiana University Research And Technology Corporation | Methods of generating human inner ear sensory epithelia and sensory neurons |
| JP7018875B2 (ja) | 2015-10-21 | 2022-02-14 | インディアナ ユニバーシティー リサーチ アンド テクノロジー コーポレーション | 多能性幹細胞からのヒト皮膚オルガノイドの誘導 |
| TWI812584B (zh) | 2015-10-30 | 2023-08-21 | 美國加利福尼亞大學董事會 | 從幹細胞產生t細胞之方法及使用該t細胞之免疫療法 |
| EP3438248A4 (en) * | 2016-03-30 | 2019-11-06 | Kyowa Hakko Bio Co., Ltd. | NAIVES PLURIPOTENT STEM CELL CULTIVATION MEDIUM AND PLURIPOTENT STEM CELL CULTIVATION PROCEDURE |
| WO2018035214A1 (en) | 2016-08-16 | 2018-02-22 | Cellular Dynamics International., Inc. | Methods for differentiating pluripotent cells |
| US11767508B2 (en) | 2017-07-24 | 2023-09-26 | Wisconsin Alumni Research Foundation | Methods and culture substrates for controlled induction of biomimetic neural tissues comprising singular rosette structures |
| WO2020047380A1 (en) | 2018-08-31 | 2020-03-05 | Wisconsin Alumni Research Foundation | Generating arterial endothelial cell-seeded vascular grafts |
| US20240219375A1 (en) | 2021-04-07 | 2024-07-04 | FUJIFILM Cellular Dynamics, Inc. | Dopaminergic precursor cells and methods of use |
| CN118339279A (zh) | 2021-10-29 | 2024-07-12 | 富士胶片细胞动力公司 | 包含突变的多巴胺能神经元及其使用方法 |
| WO2025097051A1 (en) | 2023-11-03 | 2025-05-08 | Axent Biosciences Inc. | Thermoreversible polymers with improved stability and methods and uses thereof |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4456757A (en) | 1981-03-20 | 1984-06-26 | Asahi Kasei Kogyo Kabushiki Kaisha | Isoquinolinesulfonyl derivatives and process for the preparation thereof |
| WO1997028130A1 (en) | 1996-02-02 | 1997-08-07 | Nippon Shinyaku Co., Ltd. | Isoquinoline derivatives and drugs |
| ES2286834T5 (es) | 1996-08-12 | 2011-01-31 | Mitsubishi Tanabe Pharma Corporation | Medicamentos que comprenden un inhibidor de la rho quinasa. |
| US7439064B2 (en) | 2000-03-09 | 2008-10-21 | Wicell Research Institute, Inc. | Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium |
| US20020009713A1 (en) * | 2000-05-11 | 2002-01-24 | Miller Freda D. | Methods for identifying modulators of neuronal growth |
| WO2003080649A2 (en) * | 2002-03-20 | 2003-10-02 | Regents Of The University Of Minnesota | Induction of hepatocyte proliferation in vitro by inhibition of cell cycle inhibitors |
| CA2517959A1 (en) * | 2003-03-07 | 2004-09-16 | Gamida-Cell Ltd. | Expansion of renewable stem cell populations using modulators of pi 3-kinase |
| US7829332B2 (en) * | 2004-02-13 | 2010-11-09 | Cornell Research Foundation, Inc. | Purines are self-renewal signals for neural stem cells, and purine receptor antagonists promote neuronal and glial differentiation therefrom |
| JP4576545B2 (ja) * | 2004-05-27 | 2010-11-10 | 独立行政法人理化学研究所 | 羊膜由来因子による胚性幹細胞の培養方法 |
| EP1791952A4 (en) * | 2004-08-13 | 2008-06-11 | Univ Georgia Res Found | COMPOSITIONS AND METHODS OF SELF-RENEWAL AND DIFFERENTIATION IN HUMAN EMBRYONAL STEM CELLS |
| ES2383813T3 (es) | 2004-09-08 | 2012-06-26 | Wisconsin Alumni Research Foundation | Método de cultivo y cultivo de células madre embrionarias |
| US7442548B2 (en) | 2004-09-08 | 2008-10-28 | Wisconsin Alumni Research Foundation | Culturing human embryonic stem cells in medium containing pipecholic acid and gamma amino butyric acid |
| US7641897B2 (en) * | 2004-11-23 | 2010-01-05 | The Board Of Trustees Of The Leland Stanford Junior University | Feeder layer and serum independent embryonic stem cells |
| ITVA20060041A1 (it) | 2006-07-05 | 2008-01-06 | Dialectica Srl | Uso di composti derivati amminotiazolici, di loro composizioni farmaceutiche, nel trattamento di malattie caratterizzate dalla anormale repressione della trascrizione genica, particolarmente il morbo di huntington |
| CA2658764A1 (en) | 2006-07-20 | 2008-01-24 | Mehmet Kahraman | Benzothiophene inhibitors of rho kinase |
| JP2008099662A (ja) * | 2006-09-22 | 2008-05-01 | Institute Of Physical & Chemical Research | 幹細胞の培養方法 |
-
2008
- 2008-01-17 JP JP2009546511A patent/JP5419279B2/ja active Active
- 2008-01-17 SE SE0950586A patent/SE0950586L/sv not_active Application Discontinuation
- 2008-01-17 GB GB0914193A patent/GB2458863B/en active Active
- 2008-01-17 CA CA2675445A patent/CA2675445C/en active Active
- 2008-01-17 US US12/016,066 patent/US7892830B2/en active Active
- 2008-01-17 WO PCT/US2008/051347 patent/WO2008089351A1/en not_active Ceased
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