CA2675445C - Improved culture of stem cells - Google Patents
Improved culture of stem cells Download PDFInfo
- Publication number
- CA2675445C CA2675445C CA2675445A CA2675445A CA2675445C CA 2675445 C CA2675445 C CA 2675445C CA 2675445 A CA2675445 A CA 2675445A CA 2675445 A CA2675445 A CA 2675445A CA 2675445 C CA2675445 C CA 2675445C
- Authority
- CA
- Canada
- Prior art keywords
- culture
- pluripotent stem
- cells
- agent
- stem cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Gynecology & Obstetrics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Developmental Biology & Embryology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Reproductive Health (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US88074707P | 2007-01-17 | 2007-01-17 | |
| US60/880,747 | 2007-01-17 | ||
| PCT/US2008/051347 WO2008089351A1 (en) | 2007-01-17 | 2008-01-17 | Improved culture of stem cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2675445A1 CA2675445A1 (en) | 2008-07-24 |
| CA2675445C true CA2675445C (en) | 2014-11-04 |
Family
ID=39277243
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2675445A Active CA2675445C (en) | 2007-01-17 | 2008-01-17 | Improved culture of stem cells |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7892830B2 (enExample) |
| JP (1) | JP5419279B2 (enExample) |
| CA (1) | CA2675445C (enExample) |
| GB (1) | GB2458863B (enExample) |
| SE (1) | SE0950586L (enExample) |
| WO (1) | WO2008089351A1 (enExample) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4667241B2 (ja) * | 2003-06-27 | 2011-04-06 | 旭化成株式会社 | 細胞分化抑制剤、これを用いた細胞培養方法、培養液及び培養された細胞株 |
| WO2007026353A2 (en) | 2005-08-29 | 2007-03-08 | Technion Research & Development Foundation Ltd. | Media for culturing stem cells |
| EP2206724A1 (en) | 2005-12-13 | 2010-07-14 | Kyoto University | Nuclear reprogramming factor |
| US8278104B2 (en) | 2005-12-13 | 2012-10-02 | Kyoto University | Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2 |
| US8129187B2 (en) | 2005-12-13 | 2012-03-06 | Kyoto University | Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2 |
| US20090227032A1 (en) | 2005-12-13 | 2009-09-10 | Kyoto University | Nuclear reprogramming factor and induced pluripotent stem cells |
| DK2733203T3 (en) | 2006-08-02 | 2019-02-04 | Technion Res & Dev Foundation | PROCEDURES FOR EXPANSION OF EMBRYONAL STEM CELLS IN A SUSPENSION CULTURE |
| JP2008099662A (ja) * | 2006-09-22 | 2008-05-01 | Institute Of Physical & Chemical Research | 幹細胞の培養方法 |
| JP2008307007A (ja) * | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞 |
| US9213999B2 (en) | 2007-06-15 | 2015-12-15 | Kyoto University | Providing iPSCs to a customer |
| JP5951254B2 (ja) | 2008-03-17 | 2016-07-13 | ザ スクリプス リサーチ インスティテュート | 人工多能性幹細胞を作製するための化学的手法と遺伝的手法の組み合わせ法 |
| WO2009116840A2 (en) * | 2008-03-21 | 2009-09-24 | Industry-Academic Cooperation Foundation, Yonsei University | Chemical reagents regulating stem cell fate |
| JP5346925B2 (ja) | 2008-05-02 | 2013-11-20 | 国立大学法人京都大学 | 核初期化方法 |
| EP2291511B1 (en) | 2008-05-02 | 2017-06-21 | Cellular Dynamics International, Inc. | Method for production of mast cells from stem cells |
| US8648170B2 (en) | 2008-09-19 | 2014-02-11 | Wisconsin Alumni Research Foundation | Peptide-presenting surfaces for long-term culture of pluripotent cells |
| MX2011005288A (es) * | 2008-11-20 | 2011-06-01 | Centocor Ortho Biotech Inc | Celulas madre pluripotentes en microportadores. |
| SG172160A1 (en) | 2008-12-17 | 2011-07-28 | Scripps Research Inst | Generation and maintenance of stem cells |
| US8642334B2 (en) | 2009-02-17 | 2014-02-04 | Memorial Sloan Kettering Cancer Center | Methods of neural conversion of human embryonic stem cells |
| EP2398897B1 (en) * | 2009-02-20 | 2017-06-28 | Cellular Dynamics International, Inc. | Methods and compositions for the differentiation of stem cells |
| SG174471A1 (en) * | 2009-03-20 | 2011-10-28 | Agency Science Tech & Res | Culture of pluripotent and multipotent cells on microcarriers |
| US8193235B2 (en) * | 2009-06-12 | 2012-06-05 | University Of Kansas | Compositions and methods for establishing and maintaining stem cells in an undifferentiated state |
| MX389666B (es) * | 2009-10-16 | 2025-03-19 | Scripps Research Inst | Induccion de celulas pluripotentes. |
| EP3255142A1 (en) * | 2009-10-19 | 2017-12-13 | Cellular Dynamics International, Inc. | Cardiomyocyte production |
| KR101775926B1 (ko) * | 2009-11-04 | 2017-09-07 | 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 | 화학 물질을 이용한 에피솜 재프로그래밍 |
| CN102712896A (zh) * | 2009-11-06 | 2012-10-03 | Rnl生物技术株式会社 | 用于增殖毛囊干细胞的方法 |
| WO2011058558A2 (en) | 2009-11-12 | 2011-05-19 | Technion Research & Development Foundation Ltd. | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
| CA2782577A1 (en) * | 2009-12-02 | 2011-06-09 | Research Development Foundation | Selection of stem cell clones with defined differentiation capabilities |
| CA2800498C (en) | 2010-03-31 | 2021-11-16 | The Scripps Research Institute | Reprogramming cells |
| WO2011159726A2 (en) | 2010-06-14 | 2011-12-22 | The Scripps Research Institute | Reprogramming of cells to a new fate |
| AU2011290123B2 (en) * | 2010-08-09 | 2016-11-10 | Takeda Pharmaceutical Company Limited | Method of producing pancreatic hormone-producing cells |
| EP3056198A2 (en) * | 2010-09-16 | 2016-08-17 | MSP Co., Ltd | Use of compounds for inducing differentiation of mesenchymal stem cells to chondrocytes |
| US9181529B2 (en) | 2010-10-19 | 2015-11-10 | Cellular Dynamics International, Inc. | Titration of differentiation medium components |
| US9732319B2 (en) | 2010-12-22 | 2017-08-15 | Fate Therapeutics, Inc. | Cell culture platform for single cell sorting and enhanced reprogramming of iPSCs |
| US9334523B2 (en) * | 2011-02-14 | 2016-05-10 | Life Technologies Corporation | Substrates and methods for staining live stem cells |
| CA2831609C (en) | 2011-03-30 | 2019-06-11 | Cellular Dynamics International, Inc. | Priming of pluripotent stem cells for neural differentiation |
| WO2013009825A1 (en) | 2011-07-11 | 2013-01-17 | Cellular Dynamics International, Inc. | Methods for cell reprogramming and genome engineering |
| AU2012332269B2 (en) | 2011-11-04 | 2018-05-31 | Memorial Sloan-Kettering Cancer Center | Midbrain dopamine (DA) neurons for engraftment |
| US10238755B2 (en) | 2011-11-30 | 2019-03-26 | The Wistar Institute Of Anatomy And Biology | Methods and compositions for regulation of cell aging, carcinogenesis and reprogramming |
| WO2014165663A1 (en) | 2013-04-03 | 2014-10-09 | Cellular Dynamics International, Inc. | Methods and compositions for culturing endoderm progenitor cells in suspension |
| JP6756610B2 (ja) | 2013-04-26 | 2020-09-16 | メモリアル スローン ケタリング キャンサー センター | 多分化能細胞および多能性細胞の分化を方向付けることによって発生させる皮質介在ニューロンおよびその他のニューロン細胞 |
| CN120536341A (zh) | 2014-03-04 | 2025-08-26 | 菲特治疗公司 | 改良的重编程方法和细胞培养平台 |
| US9694036B2 (en) | 2014-03-21 | 2017-07-04 | Cellular Dynamics International, Inc. | Production of midbrain dopaminergic neurons and methods for the use thereof |
| US10444227B2 (en) | 2014-08-19 | 2019-10-15 | FUJIFILM Cellular Dynamics, Inc. | Neural networks formed from cells derived from pluripotent stem cells |
| JP6581655B2 (ja) | 2014-10-14 | 2019-09-25 | フジフィルム セルラー ダイナミクス,インコーポレイテッド | 多能性幹細胞由来ケラチノサイトの生成およびケラチノサイト培養の維持 |
| CN117737124A (zh) | 2015-10-16 | 2024-03-22 | 菲特治疗公司 | 用于诱导和维护基态多能性的平台 |
| AU2016342015B2 (en) | 2015-10-21 | 2021-07-01 | Indiana University Research And Technology Corporation | Derivation of human skin organoids from pluripotent stem cells |
| CN108291197B (zh) | 2015-10-21 | 2022-09-16 | 印第安纳大学研究与技术公司 | 生成人内耳感觉上皮和感觉神经元的方法 |
| KR102929174B1 (ko) | 2015-10-30 | 2026-02-20 | 더 리전츠 오브 더 유니버시티 오브 캘리포니아 | 줄기세포로부터 t-세포를 생산하는 방법 및 상기 t-세포를 이용한 면역치료 방법 |
| US20190127707A1 (en) * | 2016-03-30 | 2019-05-02 | Kyowa Hakko Bio Co., Ltd. | Medium for culturing naive pluripotent stem cells and method for culturing pluripotent stem cells |
| AU2017313065B2 (en) | 2016-08-16 | 2021-06-24 | Fujifilm Cellular Dynamics Inc. | Methods for differentiating pluripotent cells |
| US11767508B2 (en) | 2017-07-24 | 2023-09-26 | Wisconsin Alumni Research Foundation | Methods and culture substrates for controlled induction of biomimetic neural tissues comprising singular rosette structures |
| JP7669266B2 (ja) | 2018-08-31 | 2025-04-28 | ウィスコンシン アラムニ リサーチ ファンデーション | 動脈内皮細胞を播種した血管移植片の生成 |
| EP4319876A1 (en) | 2021-04-07 | 2024-02-14 | Fujifilm Cellular Dynamics, Inc. | Dopaminergic precursor cells and methods of use |
| AU2022376962A1 (en) | 2021-10-29 | 2024-04-18 | FUJIFILM Cellular Dynamics, Inc. | Dopaminergic neurons comprising mutations and methods of use thereof |
| WO2025097051A1 (en) | 2023-11-03 | 2025-05-08 | Axent Biosciences Inc. | Thermoreversible polymers with improved stability and methods and uses thereof |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4456757A (en) * | 1981-03-20 | 1984-06-26 | Asahi Kasei Kogyo Kabushiki Kaisha | Isoquinolinesulfonyl derivatives and process for the preparation thereof |
| PT885888E (pt) * | 1996-02-02 | 2003-12-31 | Western Therapeutics Inst D | Derivados e farmacos de isoquinolina |
| PT956865E (pt) * | 1996-08-12 | 2007-07-30 | Mitsubishi Pharma Corp | Medicamentos compreendendo inibidores da rho cinase. |
| US7439064B2 (en) * | 2000-03-09 | 2008-10-21 | Wicell Research Institute, Inc. | Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium |
| US20020009713A1 (en) * | 2000-05-11 | 2002-01-24 | Miller Freda D. | Methods for identifying modulators of neuronal growth |
| WO2003080649A2 (en) * | 2002-03-20 | 2003-10-02 | Regents Of The University Of Minnesota | Induction of hepatocyte proliferation in vitro by inhibition of cell cycle inhibitors |
| EP1601759A4 (en) * | 2003-03-07 | 2007-02-14 | Gamida Cell Ltd | EXPANSION OF RENEWABLE STEM CELL POPULATIONS USING PI-3 KINASE MODULATORS |
| US7829332B2 (en) * | 2004-02-13 | 2010-11-09 | Cornell Research Foundation, Inc. | Purines are self-renewal signals for neural stem cells, and purine receptor antagonists promote neuronal and glial differentiation therefrom |
| JP4576545B2 (ja) * | 2004-05-27 | 2010-11-10 | 独立行政法人理化学研究所 | 羊膜由来因子による胚性幹細胞の培養方法 |
| US8187878B2 (en) | 2004-08-13 | 2012-05-29 | University Of Georgia Research Foundation, Inc. | Methods for increasing definitive endoderm differentiation of pluripotent human embryonic stem cells with PI-3 kinase inhibitors |
| BRPI0514641A (pt) * | 2004-09-08 | 2008-06-17 | Wisconsin Alumni Res Found | cultivação das células tronco embriÈnica humana |
| BRPI0514778B1 (pt) * | 2004-09-08 | 2018-07-10 | Wisconsin Alumni Research Foundation | Meio para cultivar células tronco embrionárias |
| US7641897B2 (en) * | 2004-11-23 | 2010-01-05 | The Board Of Trustees Of The Leland Stanford Junior University | Feeder layer and serum independent embryonic stem cells |
| ITVA20060041A1 (it) | 2006-07-05 | 2008-01-06 | Dialectica Srl | Uso di composti derivati amminotiazolici, di loro composizioni farmaceutiche, nel trattamento di malattie caratterizzate dalla anormale repressione della trascrizione genica, particolarmente il morbo di huntington |
| BRPI0713187A2 (pt) * | 2006-07-20 | 2012-10-16 | Mehmet Kahraman | método de inibir rho-quinase, método de tratamento de doença mediada por rho-quinase, composto e composição farmacêutica |
| JP2008099662A (ja) | 2006-09-22 | 2008-05-01 | Institute Of Physical & Chemical Research | 幹細胞の培養方法 |
-
2008
- 2008-01-17 SE SE0950586A patent/SE0950586L/sv not_active Application Discontinuation
- 2008-01-17 WO PCT/US2008/051347 patent/WO2008089351A1/en not_active Ceased
- 2008-01-17 CA CA2675445A patent/CA2675445C/en active Active
- 2008-01-17 US US12/016,066 patent/US7892830B2/en active Active
- 2008-01-17 GB GB0914193A patent/GB2458863B/en active Active
- 2008-01-17 JP JP2009546511A patent/JP5419279B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP5419279B2 (ja) | 2014-02-19 |
| US20080171385A1 (en) | 2008-07-17 |
| GB0914193D0 (en) | 2009-09-16 |
| JP2010516255A (ja) | 2010-05-20 |
| SE0950586L (sv) | 2009-08-13 |
| GB2458863A (en) | 2009-10-07 |
| WO2008089351A1 (en) | 2008-07-24 |
| US7892830B2 (en) | 2011-02-22 |
| GB2458863B (en) | 2011-10-12 |
| CA2675445A1 (en) | 2008-07-24 |
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