JP2010514450A5 - - Google Patents
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- Publication number
- JP2010514450A5 JP2010514450A5 JP2009544281A JP2009544281A JP2010514450A5 JP 2010514450 A5 JP2010514450 A5 JP 2010514450A5 JP 2009544281 A JP2009544281 A JP 2009544281A JP 2009544281 A JP2009544281 A JP 2009544281A JP 2010514450 A5 JP2010514450 A5 JP 2010514450A5
- Authority
- JP
- Japan
- Prior art keywords
- probe
- region
- temperature
- nucleic acid
- target nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000523 sample Substances 0.000 claims 52
- 238000000034 method Methods 0.000 claims 27
- 238000009396 hybridization Methods 0.000 claims 15
- 150000007523 nucleic acids Chemical class 0.000 claims 14
- 102000039446 nucleic acids Human genes 0.000 claims 14
- 108020004707 nucleic acids Proteins 0.000 claims 14
- 108090000790 Enzymes Proteins 0.000 claims 11
- 102000004190 Enzymes Human genes 0.000 claims 11
- 238000002844 melting Methods 0.000 claims 11
- 230000008018 melting Effects 0.000 claims 11
- 239000007787 solid Substances 0.000 claims 11
- 239000012634 fragment Substances 0.000 claims 10
- 108060002716 Exonuclease Proteins 0.000 claims 9
- 102000013165 exonuclease Human genes 0.000 claims 9
- 238000000137 annealing Methods 0.000 claims 5
- KTWOOEGAPBSYNW-UHFFFAOYSA-N ferrocene Chemical group [Fe+2].C=1C=C[CH-]C=1.C=1C=C[CH-]C=1 KTWOOEGAPBSYNW-UHFFFAOYSA-N 0.000 claims 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical group [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims 2
- 239000010931 gold Substances 0.000 claims 2
- 229910052737 gold Inorganic materials 0.000 claims 2
- 206010011224 Cough Diseases 0.000 claims 1
- 108010006785 Taq Polymerase Proteins 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 238000010438 heat treatment Methods 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US87761106P | 2006-12-29 | 2006-12-29 | |
| PCT/US2007/089007 WO2008083259A1 (en) | 2006-12-29 | 2007-12-28 | Systems and methods for detecting nucleic acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2010514450A JP2010514450A (ja) | 2010-05-06 |
| JP2010514450A5 true JP2010514450A5 (enExample) | 2012-11-29 |
Family
ID=39368252
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009544281A Pending JP2010514450A (ja) | 2006-12-29 | 2007-12-28 | 核酸検出方法及びシステム |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20080193940A1 (enExample) |
| EP (1) | EP2069525A1 (enExample) |
| JP (1) | JP2010514450A (enExample) |
| CN (1) | CN101978070A (enExample) |
| AU (1) | AU2007339793A1 (enExample) |
| CA (1) | CA2674012A1 (enExample) |
| WO (1) | WO2008083259A1 (enExample) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX340258B (es) | 2011-01-11 | 2016-07-01 | Seegene Inc | Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto. |
| US8703653B2 (en) | 2011-02-18 | 2014-04-22 | NVS Technologies, Inc. | Quantitative, highly multiplexed detection of nucleic acids |
| CN103502471B (zh) * | 2011-02-18 | 2015-04-22 | Nvs技术股份有限公司 | 核酸的定量、高度多重检测 |
| JP5852222B2 (ja) | 2011-03-29 | 2016-02-03 | シージーン アイエヌシー | Ｐｔｏ切断及び延長−依存的切断によるターゲット核酸配列の検出 |
| US9850524B2 (en) | 2011-05-04 | 2017-12-26 | Seegene, Inc. | Detection of target nucleic acid sequences by PO cleavage and hybridization |
| MX342067B (es) * | 2011-05-04 | 2016-09-09 | Seegene Inc | Detección de secuencias de ácido nucleico objetivo por desdoblamiento e hibridización de oligonucleótido de sonda. |
| GB201107863D0 (en) * | 2011-05-11 | 2011-06-22 | Olink Ab | Method and product |
| DE102011056606B3 (de) * | 2011-12-19 | 2013-01-03 | Friz Biochem Gesellschaft Für Bioanalytik Mbh | Verfahren zur elektrochemischen Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen |
| KR20130101952A (ko) | 2012-02-02 | 2013-09-16 | 주식회사 씨젠 | Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출 |
| CA2864523C (en) * | 2012-03-05 | 2019-02-05 | Seegene, Inc. | Detection of nucleotide variation on target nucleic acid sequence by pto cleavage and extension assay |
| WO2015057008A2 (en) * | 2013-10-18 | 2015-04-23 | Seegene, Inc. | Detection of target nucleic acid sequence on solid phase by pto cleavage and extension using hcto assay |
| KR102345601B1 (ko) | 2017-09-29 | 2021-12-30 | 주식회사 씨젠 | Pto 절단 및 연장-의존적 연장 분석에 의한 타겟 핵산 서열의 검출 |
| EP4074839A1 (en) * | 2021-04-16 | 2022-10-19 | Biotype GmbH | Optimized oligonucleotide tx probe for a multiplexing analysis of nucleic acids and a multiplexing method |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5641625A (en) * | 1992-05-22 | 1997-06-24 | Isis Pharmaceuticals, Inc. | Cleaving double-stranded DNA with peptide nucleic acids |
| US5719262A (en) * | 1993-11-22 | 1998-02-17 | Buchardt, Deceased; Ole | Peptide nucleic acids having amino acid side chains |
| US5766855A (en) * | 1991-05-24 | 1998-06-16 | Buchardt, Deceased; Ole | Peptide nucleic acids having enhanced binding affinity and sequence specificity |
| US5539082A (en) * | 1993-04-26 | 1996-07-23 | Nielsen; Peter E. | Peptide nucleic acids |
| US5714331A (en) * | 1991-05-24 | 1998-02-03 | Buchardt, Deceased; Ole | Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility |
| DK51092D0 (da) * | 1991-05-24 | 1992-04-15 | Ole Buchardt | Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf |
| US5846717A (en) * | 1996-01-24 | 1998-12-08 | Third Wave Technologies, Inc. | Detection of nucleic acid sequences by invader-directed cleavage |
| GB9211979D0 (en) * | 1992-06-05 | 1992-07-15 | Buchard Ole | Uses of nucleic acid analogues |
| US5527675A (en) * | 1993-08-20 | 1996-06-18 | Millipore Corporation | Method for degradation and sequencing of polymers which sequentially eliminate terminal residues |
| DE4331012A1 (de) * | 1993-09-13 | 1995-03-16 | Bayer Ag | Nukleinsäuren-bindende Oligomere mit N-Verzweigung für Therapie und Diagnostik |
| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| GB2284209A (en) * | 1993-11-25 | 1995-05-31 | Ole Buchardt | Nucleic acid analogue-induced transcription of RNA from a double-stranded DNA template |
| US5985557A (en) * | 1996-01-24 | 1999-11-16 | Third Wave Technologies, Inc. | Invasive cleavage of nucleic acids |
| US6107470A (en) * | 1997-05-29 | 2000-08-22 | Nielsen; Peter E. | Histidine-containing peptide nucleic acids |
| US6262264B1 (en) * | 1998-06-01 | 2001-07-17 | Roche Diagnostics Corporation | Redox reversible imidazole osmium complex conjugates |
| US6290839B1 (en) * | 1998-06-23 | 2001-09-18 | Clinical Micro Sensors, Inc. | Systems for electrophoretic transport and detection of analytes |
| WO2001006016A2 (en) * | 1999-07-20 | 2001-01-25 | Clinical Micro Sensors, Inc. | Amplification of nucleic acids with electronic detection |
| US7118860B2 (en) * | 1999-10-29 | 2006-10-10 | Stratagene California | Methods for detection of a target nucleic acid by capture |
| US6350580B1 (en) * | 2000-10-11 | 2002-02-26 | Stratagene | Methods for detection of a target nucleic acid using a probe comprising secondary structure |
| US20070092880A1 (en) * | 2003-07-16 | 2007-04-26 | Crothers Donald M | Invasive cleavage reaction with electrochemical readout |
| WO2005068660A1 (en) * | 2003-12-19 | 2005-07-28 | Beckman Coulter, Inc. | Solid-phase multiplexed invader assay |
| EP1838880A2 (en) * | 2005-01-21 | 2007-10-03 | Third Wave Technologies, Inc. | Methods and compositions for increased dynamic range detection of nucleic acid molecules |
| US20070099211A1 (en) * | 2005-07-15 | 2007-05-03 | Vissarion Aivazachvili | Detection of nucleic acid amplification |
-
2007
- 2007-12-28 WO PCT/US2007/089007 patent/WO2008083259A1/en not_active Ceased
- 2007-12-28 EP EP07870017A patent/EP2069525A1/en not_active Ceased
- 2007-12-28 CN CN2007800362797A patent/CN101978070A/zh active Pending
- 2007-12-28 CA CA002674012A patent/CA2674012A1/en not_active Abandoned
- 2007-12-28 AU AU2007339793A patent/AU2007339793A1/en not_active Abandoned
- 2007-12-28 US US11/965,807 patent/US20080193940A1/en not_active Abandoned
- 2007-12-28 JP JP2009544281A patent/JP2010514450A/ja active Pending