JP2010514450A - 核酸検出方法及びシステム - Google Patents

核酸検出方法及びシステム Download PDF

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Publication number
JP2010514450A
JP2010514450A JP2009544281A JP2009544281A JP2010514450A JP 2010514450 A JP2010514450 A JP 2010514450A JP 2009544281 A JP2009544281 A JP 2009544281A JP 2009544281 A JP2009544281 A JP 2009544281A JP 2010514450 A JP2010514450 A JP 2010514450A
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probe
region
nucleic acid
hybridization probe
temperature
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JP2010514450A5 (enExample
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アイヴァザクヴィル・ヴィサリオン
スカボー・クリスティアン
シュピエール・ユーゲン
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アプライド バイオシステムズ インコーポレイテッド
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Publication of JP2010514450A publication Critical patent/JP2010514450A/ja
Publication of JP2010514450A5 publication Critical patent/JP2010514450A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2009544281A 2006-12-29 2007-12-28 核酸検出方法及びシステム Pending JP2010514450A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US87761106P 2006-12-29 2006-12-29
PCT/US2007/089007 WO2008083259A1 (en) 2006-12-29 2007-12-28 Systems and methods for detecting nucleic acids

Publications (2)

Publication Number Publication Date
JP2010514450A true JP2010514450A (ja) 2010-05-06
JP2010514450A5 JP2010514450A5 (enExample) 2012-11-29

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Family Applications (1)

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JP2009544281A Pending JP2010514450A (ja) 2006-12-29 2007-12-28 核酸検出方法及びシステム

Country Status (7)

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US (1) US20080193940A1 (enExample)
EP (1) EP2069525A1 (enExample)
JP (1) JP2010514450A (enExample)
CN (1) CN101978070A (enExample)
AU (1) AU2007339793A1 (enExample)
CA (1) CA2674012A1 (enExample)
WO (1) WO2008083259A1 (enExample)

Cited By (1)

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Publication number Priority date Publication date Assignee Title
JP2014511178A (ja) * 2011-02-18 2014-05-15 エヌブイエス テクノロジーズ，インコーポレイティド 核酸の定量的かつ高度に多重化された検出

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MX340258B (es) 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
US8703653B2 (en) 2011-02-18 2014-04-22 NVS Technologies, Inc. Quantitative, highly multiplexed detection of nucleic acids
JP5852222B2 (ja) 2011-03-29 2016-02-03 シージーン アイエヌシー Ｐｔｏ切断及び延長−依存的切断によるターゲット核酸配列の検出
US9850524B2 (en) 2011-05-04 2017-12-26 Seegene, Inc. Detection of target nucleic acid sequences by PO cleavage and hybridization
MX342067B (es) * 2011-05-04 2016-09-09 Seegene Inc Detección de secuencias de ácido nucleico objetivo por desdoblamiento e hibridización de oligonucleótido de sonda.
GB201107863D0 (en) * 2011-05-11 2011-06-22 Olink Ab Method and product
DE102011056606B3 (de) * 2011-12-19 2013-01-03 Friz Biochem Gesellschaft Für Bioanalytik Mbh Verfahren zur elektrochemischen Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
CA2864523C (en) * 2012-03-05 2019-02-05 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence by pto cleavage and extension assay
WO2015057008A2 (en) * 2013-10-18 2015-04-23 Seegene, Inc. Detection of target nucleic acid sequence on solid phase by pto cleavage and extension using hcto assay
KR102345601B1 (ko) 2017-09-29 2021-12-30 주식회사 씨젠 Pto 절단 및 연장-의존적 연장 분석에 의한 타겟 핵산 서열의 검출
EP4074839A1 (en) * 2021-04-16 2022-10-19 Biotype GmbH Optimized oligonucleotide tx probe for a multiplexing analysis of nucleic acids and a multiplexing method

Citations (3)

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JP2003530822A (ja) * 1999-07-20 2003-10-21 クリニカル・マイクロ・センサーズ・インコーポレイテッド 電子的核酸検出の増幅
WO2005010199A2 (en) * 2003-07-16 2005-02-03 Geneohm Sciences, Inc. Invasive cleavage reaction with electrochemical readout
US20050255512A1 (en) * 1999-10-29 2005-11-17 Stratagene California Methods for detection of a target nucleic acid by capture

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US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5641625A (en) * 1992-05-22 1997-06-24 Isis Pharmaceuticals, Inc. Cleaving double-stranded DNA with peptide nucleic acids
US5719262A (en) * 1993-11-22 1998-02-17 Buchardt, Deceased; Ole Peptide nucleic acids having amino acid side chains
US5766855A (en) * 1991-05-24 1998-06-16 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity and sequence specificity
US5539082A (en) * 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5714331A (en) * 1991-05-24 1998-02-03 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility
DK51092D0 (da) * 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
GB9211979D0 (en) * 1992-06-05 1992-07-15 Buchard Ole Uses of nucleic acid analogues
US5527675A (en) * 1993-08-20 1996-06-18 Millipore Corporation Method for degradation and sequencing of polymers which sequentially eliminate terminal residues
DE4331012A1 (de) * 1993-09-13 1995-03-16 Bayer Ag Nukleinsäuren-bindende Oligomere mit N-Verzweigung für Therapie und Diagnostik
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
GB2284209A (en) * 1993-11-25 1995-05-31 Ole Buchardt Nucleic acid analogue-induced transcription of RNA from a double-stranded DNA template
US5985557A (en) * 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US6107470A (en) * 1997-05-29 2000-08-22 Nielsen; Peter E. Histidine-containing peptide nucleic acids
US6262264B1 (en) * 1998-06-01 2001-07-17 Roche Diagnostics Corporation Redox reversible imidazole osmium complex conjugates
US6290839B1 (en) * 1998-06-23 2001-09-18 Clinical Micro Sensors, Inc. Systems for electrophoretic transport and detection of analytes
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
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Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
JP2003530822A (ja) * 1999-07-20 2003-10-21 クリニカル・マイクロ・センサーズ・インコーポレイテッド 電子的核酸検出の増幅
US20050255512A1 (en) * 1999-10-29 2005-11-17 Stratagene California Methods for detection of a target nucleic acid by capture
WO2005010199A2 (en) * 2003-07-16 2005-02-03 Geneohm Sciences, Inc. Invasive cleavage reaction with electrochemical readout

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014511178A (ja) * 2011-02-18 2014-05-15 エヌブイエス テクノロジーズ，インコーポレイティド 核酸の定量的かつ高度に多重化された検出

Also Published As

Publication number Publication date
AU2007339793A1 (en) 2008-07-10
CN101978070A (zh) 2011-02-16
WO2008083259A1 (en) 2008-07-10
CA2674012A1 (en) 2008-07-10
EP2069525A1 (en) 2009-06-17
US20080193940A1 (en) 2008-08-14

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