JP2010514429A5 - - Google Patents
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- Publication number
- JP2010514429A5 JP2010514429A5 JP2009543399A JP2009543399A JP2010514429A5 JP 2010514429 A5 JP2010514429 A5 JP 2010514429A5 JP 2009543399 A JP2009543399 A JP 2009543399A JP 2009543399 A JP2009543399 A JP 2009543399A JP 2010514429 A5 JP2010514429 A5 JP 2010514429A5
- Authority
- JP
- Japan
- Prior art keywords
- mrsa
- probe
- fluorescent moiety
- sample
- primers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000523 sample Substances 0.000 claims 41
- 230000003321 amplification Effects 0.000 claims 15
- 238000003199 nucleic acid amplification method Methods 0.000 claims 15
- 238000000034 method Methods 0.000 claims 13
- 150000007523 nucleic acids Chemical group 0.000 claims 11
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims 9
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 8
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 claims 4
- 241000191967 Staphylococcus aureus Species 0.000 claims 4
- 229960003085 meticillin Drugs 0.000 claims 4
- 239000002773 nucleotide Substances 0.000 claims 4
- 125000003729 nucleotide group Chemical group 0.000 claims 4
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 2
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 claims 2
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 claims 2
- 230000015572 biosynthetic process Effects 0.000 claims 2
- 210000000349 chromosome Anatomy 0.000 claims 2
- 238000001514 detection method Methods 0.000 claims 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical group O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims 2
- 238000002372 labelling Methods 0.000 claims 2
- 238000002844 melting Methods 0.000 claims 2
- 230000008018 melting Effects 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 239000012472 biological sample Substances 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 210000001124 body fluid Anatomy 0.000 claims 1
- 239000010839 body fluid Substances 0.000 claims 1
- 210000001217 buttock Anatomy 0.000 claims 1
- 230000002759 chromosomal effect Effects 0.000 claims 1
- 239000013068 control sample Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 238000009396 hybridization Methods 0.000 claims 1
- 108010068698 spleen exonuclease Proteins 0.000 claims 1
- 229940035893 uracil Drugs 0.000 claims 1
- 210000002700 urine Anatomy 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US88279906P | 2006-12-29 | 2006-12-29 | |
| US60/882,799 | 2006-12-29 | ||
| EP07008206.0 | 2007-04-23 | ||
| EP07008206 | 2007-04-23 | ||
| PCT/EP2007/011458 WO2008080620A1 (en) | 2006-12-29 | 2007-12-28 | Methods for detecting methicillin-resistant s. aureus as well as primers, probes and kits for the same |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010514429A JP2010514429A (ja) | 2010-05-06 |
| JP2010514429A5 true JP2010514429A5 (enExample) | 2010-11-25 |
| JP5203387B2 JP5203387B2 (ja) | 2013-06-05 |
Family
ID=38473002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009543399A Active JP5203387B2 (ja) | 2006-12-29 | 2007-12-28 | メチシリン耐性黄色ブドウ球菌の検出方法ならびにその検出用プライマー、プローブおよびキット |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP2118306B1 (enExample) |
| JP (1) | JP5203387B2 (enExample) |
| CN (1) | CN101680023B (enExample) |
| CA (1) | CA2673958C (enExample) |
| ES (1) | ES2403587T3 (enExample) |
| WO (1) | WO2008080620A1 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2348042A1 (en) | 2001-06-04 | 2002-12-04 | Ann Huletsky | Sequences for detection and identification of methicillin-resistant staphylococcus aureus |
| US11834720B2 (en) | 2005-10-11 | 2023-12-05 | Geneohm Sciences, Inc. | Sequences for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) of MREJ types xi to xx |
| JP5503552B2 (ja) | 2007-12-21 | 2014-05-28 | バイオメリュー・エスエイ | メチシリン耐性黄色ブドウ球菌の検出 |
| JP5550060B2 (ja) * | 2008-10-03 | 2014-07-16 | 独立行政法人産業技術総合研究所 | そうか病病原菌種のｐｃｒ定量用試薬キット |
| CN104169437B (zh) | 2011-12-23 | 2017-07-11 | 生物梅里埃公司 | 甲氧西林抗性金黄色葡萄球菌的mecA变体菌株的检测 |
| EP2834375B1 (en) * | 2012-04-06 | 2021-05-05 | Geneohm Sciences Canada, Inc. | Sequences for detection and identification of methicillin-resistant staphylococcus aureus (mrsa) of mrej type xxi |
| US9080217B2 (en) * | 2012-09-21 | 2015-07-14 | Roche Molecular Systems, Inc. | Methods for detection of Clostridium difficile |
| CN108498806A (zh) * | 2018-04-13 | 2018-09-07 | 四川大学 | 一种反义肽核酸-dna四面体载体复合物及其制备方法和应用 |
| WO2020111713A1 (ko) * | 2018-11-26 | 2020-06-04 | 재단법인 바이오나노헬스가드연구단 | 황색포도상구균-특이 핵산 프로브 및 이의 용도 |
| CN111549151A (zh) * | 2020-05-06 | 2020-08-18 | 南京实践医学检验有限公司 | 一种用于检测多种细菌耐药基因的试剂盒 |
| KR102714821B1 (ko) * | 2023-07-27 | 2024-10-14 | 드림디엑스 주식회사 | 항생제 내성 세균 검출용 조성물 및 이를 이용한 검출방법 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2348042A1 (en) * | 2001-06-04 | 2002-12-04 | Ann Huletsky | Sequences for detection and identification of methicillin-resistant staphylococcus aureus |
| ATE322559T1 (de) * | 2003-11-07 | 2006-04-15 | Fed Rep Of Germany Repr By The | Verfahren zur identifizierung von methicilin resistente stapylococcus aureus (mrsa) |
| US20060252069A1 (en) * | 2005-04-21 | 2006-11-09 | Zhang Kunyan | Pcr for mrsa sccmec typing |
| CA2616241C (en) * | 2005-07-25 | 2012-02-07 | Asm Scientific, Inc. | Methods for multiplexing recombinase polymerase amplification |
-
2007
- 2007-12-28 ES ES07857150T patent/ES2403587T3/es active Active
- 2007-12-28 WO PCT/EP2007/011458 patent/WO2008080620A1/en not_active Ceased
- 2007-12-28 CN CN200780051863.XA patent/CN101680023B/zh active Active
- 2007-12-28 EP EP07857150A patent/EP2118306B1/en active Active
- 2007-12-28 CA CA2673958A patent/CA2673958C/en active Active
- 2007-12-28 JP JP2009543399A patent/JP5203387B2/ja active Active
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