CA2673958C - Methods for detecting methicillin-resistant s. aureus as well as primers, probes and kits for the same - Google Patents

Methods for detecting methicillin-resistant s. aureus as well as primers, probes and kits for the same Download PDF

Info

Publication number
CA2673958C
CA2673958C CA2673958A CA2673958A CA2673958C CA 2673958 C CA2673958 C CA 2673958C CA 2673958 A CA2673958 A CA 2673958A CA 2673958 A CA2673958 A CA 2673958A CA 2673958 C CA2673958 C CA 2673958C
Authority
CA
Canada
Prior art keywords
mrsa
seq
direct
fwd
primers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2673958A
Other languages
English (en)
French (fr)
Other versions
CA2673958A1 (en
Inventor
Christian Aichinger
Astrid Reiser
James R. Uhl
Franklin R. Cockerill
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Mayo Clinic in Florida
Original Assignee
F Hoffmann La Roche AG
Mayo Clinic in Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG, Mayo Clinic in Florida filed Critical F Hoffmann La Roche AG
Publication of CA2673958A1 publication Critical patent/CA2673958A1/en
Application granted granted Critical
Publication of CA2673958C publication Critical patent/CA2673958C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2673958A 2006-12-29 2007-12-28 Methods for detecting methicillin-resistant s. aureus as well as primers, probes and kits for the same Active CA2673958C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US88279906P 2006-12-29 2006-12-29
US60/882,799 2006-12-29
EP07008206.0 2007-04-23
EP07008206 2007-04-23
PCT/EP2007/011458 WO2008080620A1 (en) 2006-12-29 2007-12-28 Methods for detecting methicillin-resistant s. aureus as well as primers, probes and kits for the same

Publications (2)

Publication Number Publication Date
CA2673958A1 CA2673958A1 (en) 2008-07-10
CA2673958C true CA2673958C (en) 2014-04-08

Family

ID=38473002

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2673958A Active CA2673958C (en) 2006-12-29 2007-12-28 Methods for detecting methicillin-resistant s. aureus as well as primers, probes and kits for the same

Country Status (6)

Country Link
EP (1) EP2118306B1 (enExample)
JP (1) JP5203387B2 (enExample)
CN (1) CN101680023B (enExample)
CA (1) CA2673958C (enExample)
ES (1) ES2403587T3 (enExample)
WO (1) WO2008080620A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2348042A1 (en) 2001-06-04 2002-12-04 Ann Huletsky Sequences for detection and identification of methicillin-resistant staphylococcus aureus
US11834720B2 (en) 2005-10-11 2023-12-05 Geneohm Sciences, Inc. Sequences for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) of MREJ types xi to xx
JP5503552B2 (ja) 2007-12-21 2014-05-28 バイオメリュー・エスエイ メチシリン耐性黄色ブドウ球菌の検出
JP5550060B2 (ja) * 2008-10-03 2014-07-16 独立行政法人産業技術総合研究所 そうか病病原菌種のｐｃｒ定量用試薬キット
CN104169437B (zh) 2011-12-23 2017-07-11 生物梅里埃公司 甲氧西林抗性金黄色葡萄球菌的mecA变体菌株的检测
EP2834375B1 (en) * 2012-04-06 2021-05-05 Geneohm Sciences Canada, Inc. Sequences for detection and identification of methicillin-resistant staphylococcus aureus (mrsa) of mrej type xxi
US9080217B2 (en) * 2012-09-21 2015-07-14 Roche Molecular Systems, Inc. Methods for detection of Clostridium difficile
CN108498806A (zh) * 2018-04-13 2018-09-07 四川大学 一种反义肽核酸-dna四面体载体复合物及其制备方法和应用
WO2020111713A1 (ko) * 2018-11-26 2020-06-04 재단법인 바이오나노헬스가드연구단 황색포도상구균-특이 핵산 프로브 및 이의 용도
CN111549151A (zh) * 2020-05-06 2020-08-18 南京实践医学检验有限公司 一种用于检测多种细菌耐药基因的试剂盒
KR102714821B1 (ko) * 2023-07-27 2024-10-14 드림디엑스 주식회사 항생제 내성 세균 검출용 조성물 및 이를 이용한 검출방법

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2348042A1 (en) * 2001-06-04 2002-12-04 Ann Huletsky Sequences for detection and identification of methicillin-resistant staphylococcus aureus
ATE322559T1 (de) * 2003-11-07 2006-04-15 Fed Rep Of Germany Repr By The Verfahren zur identifizierung von methicilin resistente stapylococcus aureus (mrsa)
US20060252069A1 (en) * 2005-04-21 2006-11-09 Zhang Kunyan Pcr for mrsa sccmec typing
CA2616241C (en) * 2005-07-25 2012-02-07 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification

Also Published As

Publication number Publication date
CN101680023B (zh) 2014-07-23
CA2673958A1 (en) 2008-07-10
EP2118306B1 (en) 2013-02-13
ES2403587T3 (es) 2013-05-20
JP5203387B2 (ja) 2013-06-05
WO2008080620A1 (en) 2008-07-10
CN101680023A (zh) 2010-03-24
JP2010514429A (ja) 2010-05-06
EP2118306A1 (en) 2009-11-18

Similar Documents

Publication Publication Date Title
US8535888B2 (en) Compositions and methods for detecting methicillin-resistant S. aureus
CA2673958C (en) Methods for detecting methicillin-resistant s. aureus as well as primers, probes and kits for the same
Barken et al. Advances in nucleic acid-based diagnostics of bacterial infections
JP5665548B2 (ja) クロストリジウム・ディフィシレの検出
JP5986043B2 (ja) クラミジア・トラコマチスを検出するためのプライマー及びプローブ配列
US9719133B2 (en) Qualitative and quantitative detection of microbial nucleic acids
CA2802548C (en) Qualitative and quantitative detection of microbial nucleic acids
CA2787958A1 (en) Methods for identifying drug resistant mycobacterium
EP1338656A1 (en) Detection of group A streptococcus
US9816143B2 (en) Compositions and methods for detection of Staphylococcus aureus
US8097413B2 (en) Detection of group B Streptococcus
US10190178B2 (en) Compositions and methods for detecting mecC-containing methicillin-resistant Staphylococcus aureus
US20030165866A1 (en) Detection of bordetella
US7691571B2 (en) Detection of bordetella
US20110189665A1 (en) Methods for detecting drug-resistant microbes
HK1142099B (en) Primers, probes and kits for detecting methicillin-resistant s. aureus
HK1142099A1 (en) Primers, probes and kits for detecting methicillin-resistant s. aureus
JP2007195421A (ja) メタロ−β−ラクタマーゼ遺伝子検出用のプライマー、メタロ−β−ラクタマーゼ遺伝子の検出方法及びβ−ラクタム薬耐性菌の検出方法
EP4547873A1 (en) Compositions and methods for detecting lymphogranuloma venereum (lgv) serovars of chlamydia trachomatis
US20100092949A1 (en) Methods for detecting staphylococcus aureus

Legal Events

Date Code Title Description
EEER Examination request