JP2010215574A - Melanogenesis inhibitor, and skin care preparation or cosmetic product - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a melanogenesis inhibitor having excellent safety and stability and high melanogenesis inhibiting action, and a skin care preparation or a cosmetic product, especially a skin care preparation or cosmetic product for skin whitening use containing the inhibitor. <P>SOLUTION: The melanogenesis inhibitor contains a solvent extract of a mycelium or fruit body of Pleurotus eryngii belonging to genus Pleurotus, family Pleurotaceae. The skin care preparation or the cosmetic product, especially the skin care preparation or the cosmetic product for skin whitening use containing the inhibitor has excellent stability and safety, exhibits melanogenesis inhibiting action and gives high skin whitening effect. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

The present invention relates to a novel melanin production inhibitor, and a skin external preparation or cosmetic containing the same.

  Various plant-derived components have been used as active ingredients for external preparations for skin or cosmetics. For example, it is known that a specific mushroom fruit body, a culture solution or an extract of cultured mycelium has a whitening effect, and it is also proposed to be blended into a cosmetic or skin external preparation for whitening purposes. (Patent Documents 1 to 6). In addition, the types of mushrooms that have been reported are wide, and Patent Document 5 includes xymediaceae, octopus kinaceae, mannenaceae, cypressaceae, oyster mushrooms, agaricaceae, cypress mushrooms, tobacco mushrooms, dust mushrooms, nymph mushrooms, Fusentaceae is listed. In addition, Patent Document 3 describes that the melanin production inhibitor is formed by combining an eringi extract and other plant extracts, but the specification describes the melanin production inhibition in the eringi extract itself. There is no specific description that the melanin production inhibitory action is further increased by combining eringi extract with other plant extracts, and the description that eringi extract has a whitening effect I can't find any. Thus, although the above-mentioned patent document describes the effect of whitening action and the like for mushroom extracts, there is no description specifically showing the effect of melanin production inhibiting action and whitening action for eringi. Absent.

JP 05-186324 A JP 2002-322044 A JP 2003-104835 A JP 2004-285058 A JP 2005-239644 A JP 2006-083064 A

The challenge to solve the problem

  Conventionally, cosmetics such as emulsions, creams, lotions, packs, cleaning agents, etc. and skin external preparations such as dispersions, ointments, external liquids (lotions), etc., are intended to give them a predetermined medicinal effect. As a medicinal ingredient has been added. For example, whitening agents such as ascorbic acids, placenta extract, glutathione, hydroquinones and the like are added to prevent or improve skin blackening caused by sunburn or the like, stains caused by pigmentation, buckwheat etc. However, with these whitening agents, the whitening effect is not sufficient, or the desired medicinal effect is often not obtained due to deterioration in the preparation, and the improvement has been desired.

  As a result of intensive studies on the components that can be used in the external preparation for skin or cosmetics, the present inventors have found that the eringi extract has a high melanin production inhibitory action. Furthermore, it has been found that eringi extract can be easily and stably blended into a skin external preparation or cosmetic, and a skin external preparation or cosmetic with a high whitening effect due to its melanin production inhibitory action can be obtained, and the present invention has been completed. It came to do.

  The eringi extract of the present invention is excellent in safety and has a high melanin production inhibitory action. In addition, the eringi extract can be easily and stably blended into a skin external preparation or cosmetic, and when applied to the skin, a skin external preparation or cosmetic having a high whitening effect in which a melanin production inhibitory action is exhibited.

  That is, this invention relates to the melanin production inhibitor which uses an eringi extract as an active ingredient. Also, a skin external preparation or cosmetic containing an eringi extract as an active ingredient, in particular, a skin whitening external preparation excellent in prevention and improvement of pigmentation such as dullness, stains, buckwheat or senile pigment spots and liver spots. It relates to cosmetics.

Hereinafter, the present invention will be described in detail. In the present specification, “to” means a range including numerical values before and after.
The eryngii (scientific name: Pleurotus eryngii) used in the present invention is a kind of mushroom belonging to the genus Oleander, and its fruiting body is edible. The place of origin is centered on Mediterranean climatic regions such as Italy and France, as well as step climatic regions such as southern Russia and Central Asia. There is no native species of this species in Japan, and all are cultivated products. The eringi extract used in the present invention is extracted from the mycelium or fruiting body of eringi using a solvent. Although the preparation method is not particularly limited, for example, the extraction is performed at a low temperature or from room temperature to warming using a press extraction method or various appropriate solvents. In addition, the mycelium or fruit body may be subjected to an appropriate treatment such as drying, chopping, pressing, or fermentation, followed by an extraction treatment or the like, if desired.

  Examples of the extraction solvent include water, lower monohydric alcohols (methyl alcohol, ethyl alcohol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (glycerin, propylene glycol, 1, 3 -Butylene glycol, etc.), lower alkyl esters (ethyl acetate, etc.), hydrocarbons (benzene, hexane, pentane, etc.), ketones (acetone, methyl ethyl ketone, etc.), ethers (diethyl ether, tetrahydrofuran, dipropyl ether, etc.), acetonitrile Etc., and one or more of these can be used. As an example of a preferable extraction method, there may be mentioned a method in which water or ethyl alcohol having a water content of 0 to 100 vol% is used, and extraction is performed at room temperature or after heating for 1 to 5 days, followed by filtration.

  Further, the extract may be any of molecular weight fraction, solvent fraction, purified by various resin treatments (ion exchange resin, adsorbent, etc.), freeze-dried, etc.

  The content of the eringi extract of the present invention in the external preparation for skin or cosmetic is preferably 0.00001 to 1% by mass (hereinafter simply referred to as “%”), more preferably 0.01 to 0 as a dry matter. .5%. Within this range, the eringi extract can be blended stably and a high whitening effect can be exhibited. Moreover, when using an extract, if the content of the dry part which is a solute is in the said range, the extract concentration will not be limited at all.

  In addition to the above essential components, the skin external preparation or cosmetic of the present invention, in addition to the above-mentioned essential components, various components usually used in cosmetics, quasi-drugs, external medicines, etc., that is, water, alcohol, oils, surfactants , Thickeners, powders, chelating agents, pH adjusters, various medicinal agents, extracts derived from animals, plants and microorganisms, fragrances, and the like can be appropriately added within a range not impairing the effects of the present invention. In particular, other whitening agents, UV protection agents, antibacterial agents, anti-inflammatory agents, cell activators, active oxygen scavengers, moisturizers and other medicinal ingredients are used in combination to further enhance the effect of the present invention, or other Further effects can be added.

  Examples of whitening agents that can be used include ascorbic acid or derivatives thereof, arbutin, linoleic acid, vitamin E and derivatives thereof, glycyrrhizic acid and derivatives thereof, tranexamic acid, placenta extract, chamomile extract, licorice extract, age extract , Ogon extract, seaweed extract, cucumber extract, caquette extract, gokahi extract, rice bran extract, wheat germ extract, saicin extract, hawthorn extract, sun penz extract, shirayuri extract, peony extract , Sempukuka extract, soybean extract, tea extract, molasses extract, juniper extract, grape extract, hop extract, micaika extract, mokka extract, yukinoshita extract and the like.

  Examples of the UV protection agent include paramethoxycinnamate-2-ethylhexyl, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5 sodium sulfate, 4-t-butyl-4′- Examples thereof include methoxydibenzoylmethane, 2-phenyl-benzimidazole-5-sulfuric acid, titanium oxide, and zinc oxide.

  Examples of the antibacterial agent include benzoic acid, sodium benzoate, p-hydroxybenzoate ester, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol, and the like.

  Anti-inflammatory agents have the effect of suppressing inflammation such as hot flashes and erythema on the skin after sunburn. For example, sulfur and its derivatives, glycyrrhizic acid and its derivatives, glycyrrhetinic acid and its derivatives, Altea extract, Ashitaba extract , Arnica extract, Ginseng extract, nettle extract, Duckweed extract, Hypericum extract, Chamomile extract, Snapdragon extract, Watercress extract, Comfrey extract, Salvia extract, Bamboo extract, Perilla extract , Birch extract, gentian extract and the like.

  Cell activators are used for the purpose of improving rough skin, for example, caffeine, chicken crown extract, shell extract, shell meat extract, royal jelly, silk protein and its degradation products or derivatives thereof, lactoferrin or its degradation products. , Mucopolysaccharides such as chondroitin sulfate and hyaluronic acid or their salts, collagen, yeast extract, lactic acid bacteria extract, bifidobacteria extract, fermentative metabolic extract, ginkgo biloba extract, barley extract, assembly extract, lysium extract Products, carrot extract, rosemary extract, glycolic acid, citric acid, lactic acid, malic acid, tartaric acid, succinic acid and the like.

  The active oxygen scavenger has an action such as suppression of lipid peroxide production, for example, superoxide dismutase, astaxanthin, mannitol, quercetin, catechin and derivatives thereof, rutin and derivatives thereof, button pi extract, yashajitsu extract, Melissa extract, Rahan fruit extract, retinol and its derivatives, vitamin A such as carotenoid, thiamine and its derivative, riboflavin and its derivative, pyridoxine and its derivative, vitamin B such as nicotinic acid and its derivative, tocopherol and its derivative And vitamin E such as dibutylhydroxytoluene and butylhydroxyanisole.

  Examples of humectants include proteins such as elastin and keratin or derivatives thereof, hydrolysates and salts thereof, amino acids such as glycine, serine, aspartic acid, glutamic acid, arginine and theanine and derivatives thereof, sorbitol, erythritol, Trehalose, inositol, glucose, sucrose and derivatives thereof, dextrin and derivatives thereof, saccharides such as honey, D-pantenol and derivatives thereof, urea, phospholipid, ceramide, lauren extract, ginger extract, diautum extract, senkyu extract Product, mallow extract, gypsophila extract, dokudami extract, hamamelis extract, bodaige extract, maronier extract, quince extract and the like.

  The skin external preparation or cosmetic of the present invention is suitable for preparation as a cosmetic intended for beautifying skin, particularly as a whitening cosmetic. The form of the cosmetic is not particularly limited. For example, in addition to skin care cosmetics such as a milky lotion, cream, lotion, pack, cosmetic liquid, and detergent, cosmetics such as makeup cosmetics such as a foundation, blusher, and white powder It may be a quasi-drug. Further, it may be in the form of dispersion, ointment, liquid, aerosol, patch, poultice, liniment, etc. In the emulsifier type, in addition to the W / O type and O / W type, the O / W / O type Or a composite type such as W / O / W type.

[Production Example 1] Manufacture of eringi extract To 10 g of eringi fruiting body, 100 mL of heated purified water was added, extracted at room temperature for 3 days, filtered, and the solvent was removed to remove eringi extract (dried fraction). As 0.5 g).

[Test Example 1] Inhibition of melanin production by cell culture and cell viability test B16 melanoma cultured cells derived from mice were used. An appropriate amount of medium was taken in two 6-well petri dishes, seeded with B16 melanoma cells, and allowed to stand at 37 ° C. in a carbon dioxide concentration of 5%. On the next day, the sample preparation solution was added and mixed with the eringi extract obtained in Production Example 1 so that the final concentration was 0 (control), 10, 30, 100, 300, 1000 μg / mL. On day 5 of the culture, the medium was changed, and the sample preparation solution was added again. On the next day, the medium was removed and the cells were collected after washing with a phosphate buffer for one petri dish, and the whiteness of the cultured B16 melanoma cells was evaluated according to the following criteria.
In addition, the same test was done using L-ascorbic acid 2-glucoside known for the melanin production inhibitory effect as a comparison control of the eringi extract.

(Criteria)
+++: Very white compared to the control (no addition).
++: Clearly white with respect to the control (no addition).
+: White with respect to the control (no addition).
±: Slightly white with respect to the control (no addition).
-: The same black color as that of the control (no addition).

For the remaining one petri dish, cells were fixed in formalin, added to a 1% crystal violet solution and stained. The viable cell ratio with respect to each sample concentration was measured with a monocerator (manufactured by Olympus). The results are shown in Table 1.

(result)

  As is clear from the results in Table 1, the eringi extract has a melanin production inhibitory effect even at a low concentration compared to L-ascorbic acid 2-glucoside, and a higher melanin production inhibitory effect at the same concentration as L-ascorbic acid 2-glucoside. It was found to have It was also observed that the toxicity to B16 melanoma cultured cells was low. Therefore, the external preparation for skin or cosmetics of the present invention blended with eringi extract is safe even when applied to the skin, and exhibits an excellent melanin production inhibitory effect, and the skin becomes darkened by sunburn, A skin-beautifying effect that effectively suppresses spots, freckles, etc. can be expected.

  Hereinafter, examples of the preparation for external preparation for skin or cosmetics will be described. However, the present invention is not limited thereto.

[Example 1: Preparation of lotion]
A lotion having the following composition was prepared by the following method.
A. Components (1) to (8) are mixed and dissolved.
B. Components (9) to (14) are mixed and dissolved.
C. A is added to B and mixed to obtain a skin lotion.
(Ingredient) (%)
(1) Meadow home oil 0.1
(2) Jojoba oil 0.05
(3) Perfume appropriate amount (4) preservative appropriate amount (5) polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
(6) Polyoxyethylene isostearate (50 EO)
Hydrogenated castor oil
1.0
(7) Ethyl alcohol 8.0
(8) Tocopherol acetate * 1 0.05
(9) Dipotassium glycyrrhizinate * 2 0.1
(10) Glycerin 5.0
(11) 1,3-butylene glycol 5.0
(12) Polyethylene glycol 1500 0.1
(13) Elingi extract * 3 0.01
(14) Purified water remaining * 1: Riken Vitamin Co., Ltd. * 2: Ichimaru Falcos * 3: Obtained in Production Example 1

[Example 2: Preparation of lotion preparation]
A lotion having the following composition was prepared by the following method.
A. Components (1) to (5) are mixed and dissolved.
B. Components (6) to (13) are mixed and dissolved.
C. B was added to A and mixed to obtain a skin lotion.
(Ingredient) (%)
(1) Citric acid 0.05
(2) Sodium citrate 0.2
(3) Sodium pyrrolidonecarboxylate (50%) aqueous solution 0.5
(4) Glycerin 3.0
(5) 1,3-butylene glycol 8.0
(6) Purified water remaining amount (7) Ethyl alcohol 10.0
(8) Catechin * 1 0.0001
(9) Ascorbic acid glucoside * 2 0.3
(10) Elingi extract * 3 0.1
(11) Perfume Appropriate amount (12) Preservative Appropriate amount (13) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
* 1: Made by Mitsui Norin Co., Ltd. * 2: Made by Hayashibara Biochemical Research Co., Ltd. * 3: Obtained in Production Example 1

[Example 3: Preparation of emulsion]
An emulsion having the following composition was prepared by the following method.
A. Ingredient (10) is heat mixed and maintained at 70 ° C.
B. Ingredients (1) to (9) and (11) are heated and mixed and maintained at 70 ° C.
C. A is added to B, mixed and uniformly emulsified.
D. After cooling C, (12) to (16) were added and mixed uniformly to obtain an emulsion.
(Ingredient) (%)
(1) Polyoxyethylene (10E.O.) sorbitan monostearate 1.0
(2) Polyoxyethylene (60E.O.) sorbit tetraoleate 0.5
(3) Glyceryl monostearate 1.0
(4) Stearic acid 0.5
(5) Behenyl alcohol 0.5
(6) Squalane 8.0
(7) Ethyl alcohol 5.0
(8) Retinol palmitate * 1 0.1
(9) Stearyl glycyrrhizinate * 2 0.1
(10) Purified water Remaining amount (11) Preservative Appropriate amount (12) Carboxyvinyl polymer 0.2
(13) Sodium hydroxide 0.1
(14) Sodium hyaluronate * 3 0.1
(15) Elingi extract * 4 0.05
(16) Fragrance Appropriate amount * 1: Riken Vitamin Co., Ltd. * 2: Nikko Chemicals * 3: Nikko Chemicals * 4: Obtained in Production Example 1

[Example 4: Preparation of serum]
A serum having the following composition was prepared by the following method.
A. The components (1) to (9) are mixed and dissolved.
B. Components (10) to (19) are mixed and dissolved.
C. A is added to B and mixed to obtain a serum.
(Ingredient) (%)
(1) Glyceryl tri-2-ethylhexanoate 0.1
(2) Macadamian nut oil
0.05
(3) Olive oil
0.05
(4) Wheat germ oil
0.01
(5) Perfume appropriate amount (6) Preservative appropriate amount (7) Polyoxyethylene monooleate (20E.O.)
Sorbitan
0.4
(8) Polyoxyethylene isostearate (50 EO)
Hydrogenated castor oil
1.6
(9) Ethyl alcohol 8.0
(10) Glycerin
6.0
(11) 1,3-butylene glycol
8.0
(12) Dipropylene glycol
4.0
(13) Serine * 1
0.1
(14) Sorbitol
0.5
(15) Sodium lactate 1.0
(16) Elingi extract * 2
0.2
(17) Hydroxyethyl cellulose
0.08
(18) Sodium alginate
0.05
(19) Purified water remaining amount * 1: manufactured by Ajinomoto Co., Inc. * 2: obtained in Production Example 1

[Example 5: Preparation of foundation]
A foundation having the following composition was prepared by the following method.
A. The components (8) to (14) are mixed.
B. Components (15) to (21) are mixed.
C. Add components (1) to (7) to A and mix and disperse.
D. B is added to C and emulsified.
E. Add component (22) to D and mix.
(Ingredient) (%)
(1) Sericite
8.0
(2) Titanium oxide 10.0
(3) Bengala
1.0
(4) Yellow iron oxide
2.2
(5) Mica titanium
2.0
(6) Black iron oxide
0.1
(7) Organically modified bentonite (* 1)
0.5
(8) Long chain alkyl-containing polyoxyalkylene-modified organopolysiloxane (* 2)
2.0
(9) Polyoxyalkylene-modified organo polysiloxane (* 3)
2.0
(10) Decamethylcyclopentasiloxane
12.0
(11) Octamethylcyclopentasiloxane
10.0
(12) Silicone solution of trimethylsiloxysilicate (* 4)
3.0
(13) 2-methoxyhexyl paramethoxycinnamate
1.0
(14) Glyceryl tri-2-ethylhexanoate
5.0
(15) 1,3-butylene glycol
5.0
(16) Diglycerin
2.0
(17) Glycerin
2.0
(18) Elingi extract * 5
0.1
(19) Xanthan gum
0.2
(20) Sodium chloride
0.2
(21) Purified water Remaining amount (22) Fragrance proper amount * 1: Benton 38 (manufactured by NL Endustry)
* 2: Avil EM-90 (Gold Schmidt)
* 3: Silicon KF-6017 (manufactured by Shin-Etsu Chemical Co., Ltd.)
* 4: Silicon KF-7312J (manufactured by Shin-Etsu Chemical Co., Ltd.)
* 5: Obtained in Production Example 1

[Example 6: Preparation of ointment]
An ointment having the following composition was prepared by the following method.
A. A part of the components (3), (4), (6), (7) and (8) is heated and mixed and kept at 70 ° C.
B. Ingredients (1) and (2) are heated and mixed and maintained at 70 ° C.
C. Gradually add A to B and mix.
D. B is added to C and emulsified.
While cooling E.C, component (5) dissolved in the remainder of component (8) was added and mixed to obtain an ointment.
(Ingredient) (%)
(1) Stearic acid 18.0
(2) Cetanol 4.0
(3) Triethanolamine 2.0
(4) Glycerin 5.0
(5) Elingi extract * 1 0.2
(6) Lactic acid 1.0
(7) Preservative appropriate amount (8) Purified water remaining amount * 1: obtained in Production Example 1

  The above skin external preparations or cosmetics of Examples 1 to 6 are also excellent in stability and safety, and when applied to the skin, the melanin production inhibitory effect is exerted, preventing spots and freckles and whitening the skin. There was something.

Claims (3)

Melanin production inhibitor containing eringi (scientific name: Pleurotus eryngii) extract as an active ingredient. A skin external preparation or cosmetic comprising the melanin production inhibitor according to claim 1 as an active ingredient. A whitening skin external preparation or cosmetic comprising the melanin production inhibitor according to claim 1 as an active ingredient.