JP2006083064A - Antioxidant, beautifying and whitening agent, and skin preparation for external use comprising the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a highly safe antioxidant, a beautifying and whitening agent, and a skin preparation for external use comprising the same. <P>SOLUTION: The antioxidant and the beautifying and whitening agent comprise as effective ingredients extracts from at least one basidiomycete selected from among Pleurotus abalones, Pholiota squarrosa, Onnia orientalis, Astraeus hygrometricus and Pleurotus nebrodensis. These extracts exhibit excellent free radical-scavenging ability and are useful for suppression of oxidation of the skin and prevention/improvement of skin ageing. Moreover, these extracts have tyrosinase activity-inhibiting actions and can suppress melanogenesis. Fungi of these basidiomycetes are edible and are excellently safe, and therefore these extracts can be appropriately incorporated with the skin preparation for external use. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to an antioxidant, a whitening agent, and a skin external preparation containing the same, and particularly to an active ingredient thereof.

It is well known that active oxygen is generated by ultraviolet rays. Among such active oxygens, free radicals react with lipids to induce a chain oxidation reaction. Thus, free radicals cause amplified damage to the tissue.
Since skin is constantly exposed to oxygen and ultraviolet rays, such oxidative stress damage is one of the greatest organs. In recent years, various active oxygens generated by ultraviolet rays cause sebum and lipid peroxidation, protein denaturation, enzyme inhibition, etc., which induces skin inflammation in the short term, and in the long term, It is thought to cause aging and cancer. Active oxygen and lipid peroxide are also considered to be involved in skin diseases such as atopic dermatitis, contact dermatitis, and psoriasis. Thus, active oxygen (free radicals) is deeply related to skin aging and skin diseases.

A substance having the ability to scavenge free radicals can suppress and stop the radical chain reaction. Therefore, an external preparation for skin containing such an antioxidant can be expected to have a preventive / improving effect on skin aging (for example, spots, wrinkles, sagging) due to photooxidative stress. In addition, prevention and improvement effects can be expected as a skin external preparation for various skin diseases related to free radicals.
Vitamin E and vitamin C are free radical scavenging antioxidants in the living body. Synthetic antioxidants such as BHT and BHA are also known.
However, these antioxidant ability (free radical scavenging ability) cannot be said to be sufficient, and BHT and BHA are suspected to be carcinogenic.
Therefore, an antioxidant having excellent free radical scavenging ability and high safety to the skin has been desired.

On the other hand, pigmentation such as skin spots and freckles occurs because hormonal abnormalities and ultraviolet light stimulation trigger melanin production in epidermal pigment cells and melanin is excessively deposited in the epidermis.
As a means for preventing such stains and freckles, a method of administering and applying a substance that suppresses the production of melanin has been conventionally used. Specifically, for example, a method of administering L-ascorbic acid in a large amount, a method of injecting glutathione, etc., a method of applying kojic acid, cysteine, hydroquinone, etc. in the form of an ointment, cream, lotion, etc. locally .
However, at present, no satisfactory product has been obtained yet in terms of its effects, stability, safety and the like.

As natural antioxidants, extracts of shiitake mushroom, enokitake mushroom, shimeji mushroom, kawatake mushroom, matsutake mushroom, mannentake mushroom, rot mushroom, nameko and other basidiomycetes have been reported (patent documents 1 to 3).
However, regarding the extracts of Azumatake, Tsuchiguri, and Pleurotus nebrodensis, there is no knowledge about free radical scavenging ability, antioxidant ability, melanin production inhibitory action, or application to an external skin preparation.
Moreover, about the extract of black abalone, although the platelet aggregation inhibitory effect and the rough skin improvement effect have been reported (patent document 4), it is not known about radical scavenging ability, antioxidant ability, and melanin production inhibitory action.
As for Sugitake, although it has been reported that melanin-degrading substances are produced by culturing mycelia (Patent Document 5), there is no known radical scavenging ability, antioxidant ability, or melanin production inhibitory action. .

Japanese Patent Laid-Open No. 5-317016 JP-A-6-65575 JP 59-124984 A JP 2002-348247 A JP 2004-107284 A

  The present invention has been made in view of the above-mentioned problems of the prior art, and an object of the present invention is to provide a highly safe antioxidant having excellent free radical scavenging ability, and a skin external preparation containing the same. There is. Another object of the present invention is to provide a whitening agent having an excellent melanin production inhibitory action and high safety, and a skin external preparation containing the same.

As a result of intensive studies by the present inventors in order to achieve the above object, it has been found that an extract of a specific basidiomycete has a free radical scavenging ability and is useful as an antioxidant. Moreover, the melanin production inhibitory action was also found and the present invention was completed.
That is, the antioxidant according to the present invention contains, as an active ingredient, an extract from one or more basidiomycetes selected from black abalone, sugitake, azumatake, tsutsuguri, and Pleurotus nebrodensis.
Moreover, the antioxidant skin external preparation concerning this invention mix | blended the extract from 1 or more types of basidiomycetes chosen from Black abalone bamboo, Sugitake, Azumatake, Tsuchiguri, and Pleurotus nebrodensis.
Moreover, the skin external preparation concerning this invention mix | blended the extract from 1 or more types of basidiomycetes chosen from azumatake, a tsutsuguri, and Pleurotus nebrodensis.
The whitening agent according to the present invention contains, as an active ingredient, an extract from one or more basidiomycetes selected from black abalone, sugitake, azumatake, tsutsuguri, and Pleurotus nebrodensis.
The melanin production inhibitor according to the present invention contains, as an active ingredient, an extract from one or more basidiomycetes selected from black abalone, cedar, azumatake, tsuchiguri, and Pleurotus nebrodensis.

Extracts of black abalone, sugitake, azumatake, tsutsuguri, and Pleurotus nebrodensis have free radical scavenging ability and exert an antioxidant effect. The fruiting bodies (mushrooms) of these basidiomycetes are known as medicinal or edible and are highly safe. Therefore, the antioxidant of the present invention can be used in various fields, but can be suitably used particularly in the fields of cosmetics, pharmaceuticals, quasi drugs, foods and the like.
Moreover, the skin external preparation which mix | blended the antioxidant of this invention suppresses oxidation of skin by trapping free radicals in the skin, and is useful for prevention and improvement of skin aging such as spots, wrinkles and sagging. It can also be expected to prevent and improve free radical related skin diseases.
In addition, black abalone, sugitake, azumatake, tsutsuguri, and Pleurotus nebrodensis extracts are useful as whitening agents because they inhibit tyrosinase activity and suppress melanin production.

The scientific names and classifications of basidiomycetes, sugitake mushrooms, azumatake mushrooms, and tsuchiguri which are basidiomycetes used in the present invention are as follows.
Pleurotus abalonus: Pleurotus squarrosa: Pleurotus squarrosa: Onnia orientalis: Tobacco spp. (Astraeus hygrometricus)
Pleurotus nebrodensis is a basidiomycete belonging to the genus Oleander genus, and the official Japanese name is not fixed, but the mushroom is commercially available under the trade name of “Yukireitake” (Agricultural Union Corporation Neo Plants, (Yes) Akagi Mushroom Honpo, (Yes) Erasawa Sangyo). Further, a Pleurotus nebrodensis strain having a deposit number of FERM P-19370 can also be suitably used.
The scientific properties of FERM P-19370 are as follows.

1. Scientific properties ... Fungus characteristics: Forms white colonies under nutrient medium containing carbon and nitrogen sources. In addition, a clamp connection (a faint connection) is observed under an optical microscope.
2. Taxonomic position ... basidiomycetes 3. Culture conditions (1) Medium name: SMYA medium (S: saccharose, M: malto extract, Y: yeast extract, A: agar)
S, A → commercial product M, Y → Difco (Difco)
(2) Medium composition: per 1000 ml of medium
1% sucrose 10g
1% malt extract 10g
0.4% yeast extract 4g
2% agar 20g

(3) pH of medium: 5.0 to 7.0 (optimum pH 5.5)
(4) Sterilization condition of medium ... 121 ° C 20 minutes (5) Medium temperature ... 28 ° C
(6) Culture period: 10 days (7) Oxygen requirement ... Aerobic Storage conditions Can be stored by freezing method.
(1) Freezing conditions: -80 ° C
(2) Protective agent: 10-20% glycerin aqueous solution (optimum 20%)
(3) Restoration rate after freezing: 100% in 1 year and 99% in 3 years
5. Conditions of survival test (1) Microorganism restoration ... 40 ℃
(2) Inoculation / culture / confirmation method: Same as the culture conditions.

  The fruiting bodies (mushrooms) of the basidiomycetes of the present invention are either native or cultivated in Japan, and are used for medicinal or edible purposes. There has never been a report that it has an inhibitory action, and it has been revealed for the first time by the present inventors.

In the present invention, it is preferable to use fruit bodies (mushrooms) as the extraction site. The fruiting body may be either raw or dried, but the dried fruiting body is desirable from the viewpoints of handleability, storage stability and extraction efficiency. In addition, an effect can be expected for the mycelium. As the mycelium, raw or dried mycelium obtained by culturing inoculum in a medium containing a carbon source and a nitrogen source can be used, but a dried one is convenient.
In this invention, what was obtained by either self-growth or cultivation can be used, and the said basidiomycete extract may mix and use 2 or more types.

  Prior to obtaining the extract, it is preferred to destroy the tissue. This makes it possible to extract efficiently. As a means for destroying the tissue, pulverization processing by various pulverization mixers such as bead mill, Waring blender, homogenizer, impact crushing processing by blasting machine, physical processing such as freezing processing, ultrasonic processing, aqueous solution of sodium hydroxide, etc. There are chemical treatments such as alkali treatment with, treatment with enzymes having cell wall decomposing action such as cellulase and pectinase, and osmotic pressure treatment, and these can be carried out alone or in appropriate combination.

  In the enzyme treatment, a known cell wall degrading enzyme or polysaccharide degrading enzyme can be used, and one or two of cellulase, hemicellulase, chitinase, α- and β-glucuronidase, pectinase, xylanase, α- and β-glucanase, etc. The above can be used. For the enzyme treatment, the enzyme may be added as an aqueous solution to mushrooms that have been appropriately shredded or crushed and shaken or stirred.

  As a method for obtaining the extract of the present invention, an extraction solvent may be added to the mushroom whose tissue has been destroyed as described above, and the mixture may be appropriately stirred and extracted. The extraction solvent is not particularly limited as long as the effects of the present invention are not impaired. For example, primary alcohols such as water, methanol and ethanol, polyhydric alcohols such as 1,3-butylene glycol and propylene glycol, and lower alcohols such as ethyl acetate. Examples include alkyl esters, aromatic carbon such as benzene and hexane, ethyl ether, acetone and the like. In the present invention, two or more of these may be combined. As a preferable solvent, water, methanol, ethanol, 1,3-butylene glycol or a mixed solvent thereof is used. Note that the extraction residue may be re-extracted under the same conditions as described above, and this extraction operation may be repeated several times. The extract can be used as an extract as it is or after removing the solvent by a known appropriate method such as concentration under reduced pressure.

In the external preparation for skin of the present invention, the amount of the extract of black abalone, sugitake, azumatake, tsutsuguri, or Pleurotus nebrodensis is 0.001 to 20.0% by mass, preferably 0.01% in terms of dry mass. -10.0 mass%. When the amount is less than 0.001% by mass, the effect of the present invention is not sufficiently exhibited, and even if the amount exceeds 20.0% by mass, a further increase in the effect cannot be substantially expected. Also tend to be difficult. A Pleurotus nebrodensis extract is particularly suitable as a whitening agent.
In addition to the above essential components, the external preparation for skin of the present invention is usually used for external preparations for skin such as cosmetics and pharmaceuticals, for example, whitening agents, moisturizers, antioxidants, oily components, ultraviolet absorbers, surfactants. Agents, thickeners, alcohols, powder components, color materials, aqueous components, water, various skin nutrients, various drugs, chelating agents, pH adjusters, and the like can be appropriately blended as necessary.

The topical skin preparation of the present invention refers to those usually used in the fields of pharmaceuticals, quasi drugs, cosmetics, etc. The dosage form is not particularly limited as long as the effects of the present invention are exhibited. For example, any ointment, cream, milky lotion, lotion, pack, bath preparation and the like conventionally used for external preparations for skin may be used.
Hereinafter, the present invention will be described in more detail with reference to examples. In addition, this invention is not limited by this. The blending amount is mass% unless otherwise specified.

Antioxidant activity test of mushroom extract (1) Preparation of mushroom extract 80 ml of methanol was added to 10 g of each fruit body dry powder and allowed to stand at room temperature for 7 days to obtain a methanol extract. Next, the methanol extract was filtered with a filter paper, concentrated with an evaporator, and the residue was dried under reduced pressure to obtain a mushroom extract. Each mushroom extract was dissolved in an appropriate amount of methanol (ethanol in Azumatake, DMSO in Pleurotus nebrodensis), and used as a test solution in the following experiments. The strains used in this test are as follows.
Black abalone bamboo: FCE732
Sugitake: FCE1561
Azumatake: FCE660, FCE659, FCE661
Tsuchiguri: FCE2500
Pleurotus nebrodensis: FERM P-19370

(2) Measurement of antioxidant activity (free radical scavenging activity) Antioxidant activity was measured by the DPPH method. DPPH (1,1-Diphenyl-2-picrylhydrazyl) previously dissolved in ethanol was added to each test solution to a final concentration of 0.1 mM, stirred, allowed to stand at room temperature for 10 minutes, and absorbance at 517 nm. Was measured. The control was a measured value of only the solvent (methanol, ethanol or DMSO) not containing the extract.

(3) Results As can be seen from FIGS. 1 to 7, free radical scavenging activity was observed in each fruit body extract of black abalone, sugitake, azumatake, tsutsuguri, and Pleurotus nebrodensis. Thus, it is understood that these extracts can be used as free radical scavenging antioxidants.

Mushroom extract tyrosinase activity inhibition test (1) Preparation of mushroom extract A sample solution was prepared in the same manner as (1) of Example 1 above.
(2) Cell culture method Mouse-derived B16 melanoma culture cells were used. The cells were cultured at 37 ° C. in a CO ₂ incubator (95% air, 5% carbon dioxide) in an Eagle MEM medium containing 10% FBS and hand and / or theophylline (0.09 mg / ml). After 24 hours of culture, the sample solution was added so as to have a final concentration (concentration of extracted dry product) of 0.01 to 0.1% by mass, and the culture was further continued for 3 days. Determination and tyrosinase activity inhibitory effect were measured.

(3) Visibility determination of the amount of melanin A diffusion plate was placed on the lid of the well plate, the amount of intracellular melanin was observed with an inverted microscope, and comparison was made in the case of a sample (reference) to which no mushroom extract was added. The score and criteria are as follows.
<Score: Criteria>
-2: It was judged to be white compared with the standard and the amount of melanin was small.
-1: It was judged to be slightly white compared to the standard and slightly less melanin.
0: It was judged that the amount of melanin was almost the same level of white compared to the standard.

(4) Measurement of tyrosinase activity Before the measurement, the medium in the well was removed and washed twice with 100 μl of PBS. To each well was added 45 μl of PBS containing 1% Triton-X (Rohm and Haas, surfactant). The plate was vibrated for 1 minute to thoroughly break the cell membrane, and the absorbance at 475 nm was measured with a microplate reader to obtain the absorbance at 0 minute. Then, 5 μl of 10 mM L-DOPA solution was quickly added, transferred to an incubator at 37 ° C., and reacted for 60 minutes. The plate was shaken for 1 minute, and the absorbance (475 nm) at 60 minutes was measured. The ratio of the absorbance difference of the extract-added sample to the absorbance difference at 0 minutes and 60 minutes in the case of the sample not added with the mushroom extract (control) was defined as the tyrosinase activity rate (%).

(5) Results (Table 1)
――――――――――――――――――――――――――――――――――――
Pleurotus nebrodensis concentration (%)
0.01 0.03 0.1
――――――――――――――――――――――――――――――――――――
Visual judgment evaluation -1 -2 -2
Tyrosinase activity rate (%) 96 94 43
――――――――――――――――――――――――――――――――――――
As shown in Table 1, the mushroom extract of the present invention has excellent melanin production inhibitory action and tyrosinase activity inhibitory action, and is useful as a whitening agent.

Formulation examples of the external preparation for skin of the present invention are shown below.
Formulation Example 1 Lotion Ethyl alcohol 5
Glycerin 1
1,3-butylene glycol 5
Polyoxyethylene polyoxypropylene decyl tetradecyl ether 0.2
Sodium hexametaphosphate 0.003
Trimethylglycine 1
Sodium polyaspartate 0.1
α-tocopherol 2-L-ascorbic acid potassium phosphate diester 0.1
Thiotaurine 0.1
Green tea extract 0.1
Western mint extract 0.1
Iris root extract 0.1
Tutigriethanol extract 0.1
HEDTA3 sodium 0.1
Carboxyvinyl polymer 0.05
Potassium hydroxide 0.02
Appropriate amount of phenoxyethanol Purified water Residue Fragrance Appropriate amount

Formulation Example 2 Lotion Glycerin 2
1,3-butylene glycol 4
Erythritol 1
Polyoxyethylene methyl glucoside 1
Polyoxyethylene hydrogenated castor oil 0.5
Azumatake 50% 1,3-butylene glycol extract (dry residue concentration 1%) 0.1
Citric acid 0.02
Sodium citrate 0.08
Phenoxyethanol appropriate amount N-coconut oil fatty acid acyl L-arginine ethyl
DL-pyrrolidonecarboxylic acid 0.1
Purified water residue

Formulation Example 3 Latex Dimethylpolysiloxane 3
Decamethylcyclopentasiloxane 4
Ethanol 5
Glycerin 6
1,3-butylene glycol 5
Polyoxyethylene methyl glucoside 3
Sunflower oil 1
Squalane 2
Potassium hydroxide 0.1
Sodium hexametaphosphate 0.05
Hydroxypropyl-β-cyclodextrin 0.1
Dipotassium glycyrrhizinate 0.05
Loquat leaf extract 0.1
Sodium L-glutamate 0.05
Fennel extract 0.1
Yeast extract 0.1
Lavender oil 0.1
Giant extract 0.1
Sugitake 50% ethanol extract 0.1
Dimorpholinopyridazinone 0.1
Xanthan gum 0.1
Carboxyvinyl polymer 0.1
Acrylic acid / alkyl methacrylate copolymer (Pemulene TR-1) 0.1
Bengala appropriate amount Yellow iron oxide appropriate amount Paraben appropriate amount Purified water residue

Formulation Example 4 Latex Dimethylpolysiloxane 2
Decamethylcyclopentasiloxane 25
Dodecamethylcyclohexasiloxane 10
Polyoxyethylene / methylpolysiloxane copolymer 1.5
Trimethylsiloxysilicic acid 1
1,3-butylene glycol 5
Squalane 0.5
Talc 5
Dipotassium glycyrrhizinate 0.1
Black abalone bamboo ethanol extract 0.1
Tocopherol acetate 0.1
Edetate trisodium 0.05
4-t-butyl-4′-methoxydibenzoylmethane 1
2-methoxyhexyl paramethoxycinnamate 5
Diparamethoxycinnamic acid mono-2-ethylhexanoic acid glyceryl 1
Silicone coated fine particle titanium oxide (40 nm) 4
Dimethyl distearyl ammonium hectorite 0.5
Spherical polyethylene powder 3
Appropriate amount of phenoxyethanol Purified water Residue Fragrance Appropriate amount

Formulation Example 5 Latex Liquid Paraffin 7
Vaseline 3
Decamethylcyclopentasiloxane 2
Behenyl alcohol 1
Glycerin 5
Dipropylene glycol 7
Polyethylene glycol 1500 2
Jojoba oil 1
Isostearic acid 0.5
Stearic acid 0.5
Behenic acid 0.5
Tetra-2-ethylhexanoic acid pentaerythrit 3
Cetyl 2-ethylhexanoate 3
Glycerol monostearate 1
Polyoxyethylene glyceryl monostearate 1
Potassium hydroxide 0.1
Sodium hexametaphosphate 0.05
Stearyl glycyrrhetinate 0.05
L-Arginine 0.1
Royal Jelly Extract 0.1
Yeast extract 0.1
Sugitake ethyl acetate extract 0.01
Tocopherol acetate 0.1
Acetylated sodium hyaluronate 0.1
Edetate trisodium 0.05
4-t-butyl-4'-methoxydibenzoylmethane 0.1
2-Ethylhexyl paramethoxycinnamate 0.1
Carboxyvinyl polymer 0.15
Paraben appropriate amount Purified water Residue Fragrance appropriate amount

Formulation Example 6 Cream α-olefin oligomer 10
Vaseline 1
Microcrystalline wax 3
Decamethylcyclopentasiloxane 5
Glycerin 10
Dipropylene glycol 2
1,3-butylene glycol 2
Erythritol 2
Squalane 1
Glycerin fatty acid ester eicosannic acid condensate 0.1
Isostearic acid 1
Cetyl 2-ethylhexanoate 5
Sodium chloride 0.5
Sodium hexametaphosphate 0.05
Stearyl glycyrrhetinate 0.05
Koubo extract 0.1
L-ascorbyl magnesium phosphate 2
Tocopherol acetate 0.5
Thiotaurine 0.1
DL-pyrrolidonecarboxylate sodium 1
Turmeric extract 0.1
Tutigri 50% ethanol extract 0.1
Edetate trisodium 0.1
Dimethyl distearyl ammonium hectorite 2
Sodium carboxymethylcellulose 0.1
Paraben appropriate amount Purified water Residue Fragrance appropriate amount

Formulation Example 7 Cream Liquid Paraffin 8
Vaseline 3
Dimethylpolysiloxane 2
Stearyl alcohol 3
Behenyl alcohol 2
Glycerin 5
Dipropylene glycol 4
Trehalose 1
Tetra-2-ethylhexanoic acid pentaerythrit 4
Polyisoethylene glyceryl monoisostearate 2
Polyoxyethylene glyceryl monostearate 1
Lipophilic glyceryl monostearate 2
Citric acid 0.05
Sodium citrate 0.05
Potassium hydroxide 0.015
Oil-soluble licorice extract 0.1
Retinol palmitate (1 million units) 0.25
Tocopherol acetate 0.1
Black abalone mushroom 50% ethanol extract 0.1
P-hydroxybenzoate appropriate amount phenoxyethanol appropriate amount dibutylhydroxytoluene appropriate amount edetate trisodium 0.05
4-t-butyl-4′-methoxydibenzoylmethane 0.01
2-Ethylhexyl paramethoxycinnamate 0.1
β-carotene 0.01
Polyvinyl alcohol 0.5
Hydroxyethyl cellulose 0.5
Carboxyvinyl polymer 0.05
Purified water Residue Fragrance Appropriate amount

Formulation Example 8 Two-layer type cream dimethylpolysiloxane 5
Decamethylcyclopentasiloxane 25
Trimethylsiloxysilicic acid 5
Polyoxyethylene / methylpolysiloxane copolymer 2
Dipropylene glycol 5
Dextrin palmitate coated fine particle zinc oxide (60nm) 15
Dipotassium glycyrrhizinate 0.02
Glutathione 1
Thiotaurine 0.05
Clara extract 1
Azumatake 1,3-butylene glycol extract (dry residue concentration 0.1%) 5
Paraben appropriate amount Phenoxyethanol appropriate amount Trisodium edetate appropriate amount 2-ethylhexyl paramethoxycinnamate 7.5
Dimethyl distearyl ammonium hectorite 0.5
Spherical polyalkyl acrylate powder 5
Butylethylpropanediol 0.5
Purified water Residue Fragrance Appropriate amount

Formulation Example 9 Gel Glycerin 2
1,3-butylene glycol 4
Potassium hydroxide 0.1
Fish collagen 20
Edetic acid-3Na 0.05
Tutigri 50% 1,3-butylene glycol (dry residue concentration 1%) 1
Carboxyvinyl polymer 0.25
P-Hydroxybenzoate Equal amount Purified water Residue

Formulation Example 10 Pack Dipropylene Glycol 5.0
Polyoxyethylene (60 mol) hydrogenated castor oil 5.0
Azumatake 50% ethanol extract 0.01
Olive oil 5.0
Tocophenol acetate 0.2
Ethylparaben 0.2
Fragrance 0.2
Sodium bisulfite 0.03
Polyvinyl alcohol (degree of saponification 90, degree of polymerization 2000) 13.0
Ethanol 7.0
Purified water residue

Formulation Example 11 Emulsion Foundation Behenyl Alcohol 0.5
Dipropylene glycol 6
Stearic acid 1
Glycerol monostearate 1
Potassium hydroxide 0.2
Triethanolamine 0.8
DL-α-tocopherol acetate 0.1
P-Hydroxybenzoate Equivalent amount Yellow iron oxide 1
α-olefin oligomer 3
Dimethylpolysiloxane (6 mPa.s) 2
Dimethylpolysiloxane (100 mPa.s) 5
Batyl alcohol 0.5
Isostearic acid 1
Behenic acid 0.5
Cetyl 2-ethylhexanoate 10
Polyoxyethylene glyceryl monostearate 1
Titanium oxide 3
Mica titanium / polyalkyl acrylate composite powder 0.5
Surface-treated titanium oxide (MT-062) 10
Polyacrylic acid alkyl coated mica titanium 0.5
Black iron oxide coated mica titanium 0.5
Silicic anhydride 6
2-Ethylhexyl paramethoxycinnamate 2
Bengala appropriate amount Ultramarine blue appropriate amount Black iron oxide appropriate amount Legal dye appropriate amount Xanthan gum 0.1
Bentonite 1
Sodium carboxymethylcellulose 0.1
Black abalone bamboo ethanol extract 0.1
Purified water Residue Fragrance Appropriate amount

Formulation Example 12 Solid Foundation Talc 43.1
Kaolin 15.0
Sericite 10.0
Zinc flower 7.0
Titanium dioxide 3.8
Yellow iron oxide 2.9
Black iron oxide 0.2
Squalane 8.0
Isostearic acid 4.0
Monooleic acid POE sorbitan 3.0
Isocetyl octoate 2.0
Sugitake ethanol extract 0.5
Preservative appropriate amount Fragrance proper amount

Formulation Example 13 Latex Dimethylpolysiloxane 2
Decamethylcyclopentasiloxane 25
Dodecamethylcyclohexasiloxane 10
Polyoxyethylene / methylpolysiloxane copolymer 1.5
Trimethylsiloxysilicic acid 1
1,3-butylene glycol 5
Squalane 0.5
Talc 5
Dipotassium glycyrrhizinate 0.1
Pleurotus nebrodensis ethanol extract 0.1
Tocopherol acetate 0.1
Edetate trisodium 0.05
4-t-butyl-4′-methoxydibenzoylmethane 1
2-methoxyhexyl paramethoxycinnamate 5
Diparamethoxycinnamic acid mono-2-ethylhexanoic acid glyceryl 1
Silicone coated fine particle titanium oxide (40 nm) 4
Dimethyl distearyl ammonium hectorite 0.5
Spherical polyethylene powder 3
Appropriate amount of phenoxyethanol Purified water Residue Fragrance Appropriate amount

Formulation Example 14 Cream Liquid Paraffin 8
Vaseline 3
Dimethylpolysiloxane 2
Stearyl alcohol 3
Behenyl alcohol 2
Glycerin 5
Dipropylene glycol 4
Trehalose 1
Tetra-2-ethylhexanoic acid pentaerythrit 4
Polyisoethylene glyceryl monoisostearate 2
Polyoxyethylene glyceryl monostearate 1
Lipophilic glyceryl monostearate 2
Citric acid 0.05
Sodium citrate 0.05
Potassium hydroxide 0.015
Oil-soluble licorice extract 0.1
Retinol palmitate (1 million units) 0.25
Tocopherol acetate 0.1
Pleurotus nebrodensis 50% ethanol extract 0.1
P-hydroxybenzoate appropriate amount phenoxyethanol appropriate amount dibutylhydroxytoluene appropriate amount edetate trisodium 0.05
4-t-butyl-4′-methoxydibenzoylmethane 0.01
2-Ethylhexyl paramethoxycinnamate 0.1
β-carotene 0.01
Polyvinyl alcohol 0.5
Hydroxyethyl cellulose 0.5
Carboxyvinyl polymer 0.05
Purified water Residue Fragrance Appropriate amount

Formulation Example 15 Emulsification Foundation Behenyl Alcohol 0.5
Dipropylene glycol 6
Stearic acid 1
Glycerol monostearate 1
Potassium hydroxide 0.2
Triethanolamine 0.8
DL-α-tocopherol acetate 0.1
P-Hydroxybenzoate Equivalent amount Yellow iron oxide 1
α-olefin oligomer 3
Dimethylpolysiloxane (6 mPa.s) 2
Dimethylpolysiloxane (100 mPa.s) 5
Batyl alcohol 0.5
Isostearic acid 1
Behenic acid 0.5
Cetyl 2-ethylhexanoate 10
Polyoxyethylene glyceryl monostearate 1
Titanium oxide 3
Mica titanium / polyalkyl acrylate composite powder 0.5
Surface-treated titanium oxide (MT-062) 10
Polyacrylic acid alkyl coated mica titanium 0.5
Black iron oxide coated mica titanium 0.5
Silicic anhydride 6
2-Ethylhexyl paramethoxycinnamate 2
Bengala appropriate amount Ultramarine blue appropriate amount Black iron oxide appropriate amount Legal dye appropriate amount Xanthan gum 0.1
Bentonite 1
Sodium carboxymethylcellulose 0.1
Pleurotus nebrodensis ethanol extract 0.1
Purified water Residue Fragrance Appropriate amount

It is a figure which shows the free radical capture | acquisition activity of the black abalone mushroom fruit body extract concerning one Example of this invention. It is a figure which shows the free radical scavenging activity of the Sugitake fruiting body extract concerning one Example of this invention. It is a figure which shows the free radical scavenging activity of the Azumatake fruiting body extract concerning one Example of this invention. It is a figure which shows the free radical scavenging activity of the Azumatake fruiting body extract concerning one Example of this invention. It is a figure which shows the free radical scavenging activity of the Azumatake fruiting body extract concerning one Example of this invention. It is a figure which shows the free radical capture | acquisition activity of the cuticle fruit body extract concerning one Example of this invention. It is a figure which shows the free radical scavenging activity of the Pleurotus nebrodensis fruiting body extract concerning one Example of this invention.

Claims (5)

  An antioxidant comprising as an active ingredient an extract from one or more basidiomycetes selected from black abalone, cedar, azumatake, tsuchiguri, and Pleurotus nebrodensis.   Antioxidant skin external preparation containing an extract from one or more basidiomycetes selected from black abalone, sugitake, azumatake, tsuchiguri, and Pleurotus nebrodensis.   An external preparation for skin containing an extract from at least one basidiomycete selected from Azumatake, Tsuchiguri, and Pleurotus nebrodensis.   A whitening agent containing as an active ingredient an extract from one or more basidiomycetes selected from black abalone, sugitake, azumatake, tsuchiguri, and Pleurotus nebrodensis. A melanin production inhibitor comprising, as an active ingredient, an extract from one or more basidiomycetes selected from black abalone, cedar, azumatake, tsuchiguri, and Pleurotus nebrodensis.

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