JPH10158144A - Skin cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a skin cosmetic that contains an extract of Pleurotus eryngii, a kind of Basidiomycetes, and is excellent in skin photo-aging prevention and improvement effect. SOLUTION: Pleurotus eryngii in Pleurotus, a kind of Basidiomycetes, is cultured and its fungus bodies or fruits are extracted through a general process continuously or batchwise, preferably with a polar solvent (for example, an alcohol such as methanol or an ether such as ethyl ether) to prepare the objective skin cosmetic. This extract may be in any form, for example, homogenate, crushed product, extract or their concentrate or dried products or may be in a mixture thereof. This extract is formulated to a cosmetic, preferably in an amount of 0.001-10wt.% based on the whole cosmetic on the dry basis to provide a skin cosmetic excellent in skin photo-again prevention and improvement effect.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to skin cosmetics,
More specifically, the present invention relates to a skin cosmetic that is highly safe and has an excellent effect of preventing and improving photoaging of the skin.

[0002]

2. Description of the Related Art In general, skin aging refers to a physiological phenomenon that occurs when physiological aging associated with aging and photoaging due to exposure to sunlight (ultraviolet rays) mutually affect each other. Attention has been paid to the relationship between photoaging and pigmentation such as wrinkles, rough skin, and spots and freckles (by Gilchrest;
Photodamage: Blackwell Sci
ence, Inc. , 1995). That is, it is known that, when the sun (ultraviolet rays) is continuously applied for a long period of time, deep wrinkles of the face and neck are increased, and furthermore, the skin is dried and the skin is pigmented such as rough spots, spots and freckles. Trouble came to be regarded as a problem.

Conventionally, various ultraviolet absorbing, scattering and shielding substances such as titanium oxide, zinc oxide, paramethoxycinnamic acid ester and paraaminobenzoic acid ester have been blended in order to prevent the accelerated photoaging of the skin due to these ultraviolet rays. Cosmetics (sunscreen, sunprotect cosmetics) have been developed and used. However, even with these cosmetics, it is difficult to protect the skin from overwhelming sun exposure. It is widely known that an external application of all-trans retinoic acid is effective as a method for improving the photoaging symptoms of the skin (Gil).
by Chrest; Photodamage: Black
well Science, Inc. , 1995, etc.), cosmetics problems such as rough skin,
Further, due to safety issues, the use in Japan has been limited to physicians, and there has been a keen need for the development of highly effective and safe photoaging prevention agents and skin cosmetics.

On the other hand, attempts have been made to mix basidiomycete cultures and fruiting body extracts into cosmetics, and various effects have been obtained. However, it has not been known that the cultured fungi and fruiting bodies of Pleurotus eryngii, a basidiomycete belonging to Pleurotus genus, contain a substance having an anti-aging effect. Extracts of cultured cells and fruiting bodies have not been used in cosmetics at all.

[0005]

DISCLOSURE OF THE INVENTION The present invention has been made in view of the above, and has as its object to provide a skin cosmetic which is highly safe and has an excellent effect of preventing and improving photoaging of the skin. I do.

[0006]

Means for Solving the Problems The present inventors have made intensive studies in order to solve the above-mentioned problems, and as a result, the extract of Pleurotus eryngis has the effect of preventing and improving the photoaging of the skin, and The present invention was also found to be excellent in safety, and completed the present invention.

That is, the present invention is a skin cosmetic comprising an extract of Pleurotus eryngii. As an extract of Pleurotus eryngis contained in the skin cosmetic of the present invention,
Specific examples include an extract obtained by extracting Pleurotus erysinus with a polar solvent.

[0008] The content of the extract of Pleurotus eryngis in the skin cosmetic of the present invention, specifically, 0.001 as a dry solid content relative to the total amount of the cosmetic.
The content may be about 10 to 10% by weight.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail.
First, Pleurotus containing the skin cosmetic of the present invention.
The extract of Erinji will be described. (1) Extract of Pleurotus eryngie The skin cosmetic of the present invention contains an extract of Pleurotus eryngie. Pleutus Elingy
rotus eryngii) is one of the Basidiomycetes belonging to the genus Pleurotus
In the present invention, cultured cells, fruiting bodies, etc. obtained by culturing Pleurotus erysinus are used after being extracted.

[0010] The cultured cells and fruiting bodies of Pleurotus erysinus can be obtained from the inoculum of Pleurotus erysinus by, for example, culturing and cultivating as follows.

A cultivation bottle is filled with a medium suitable for growing Pleurotus erysinus, and the cultivation bottle filled with this medium is sterilized by a usual method such as autoclave sterilization. After sterilization, the cultivation bottle is allowed to cool,
Inoculate Erinzie inoculum. After inoculation of the inoculum, the cultivation bottle is placed in a cultivation room where the temperature, humidity, light amount and the like are adjusted to a range of conditions suitable for cultivation of Pleurotus erysinus, for example, a temperature of about 13 to 15 ° C., and sufficient aging is performed. And ripening by culturing the cells for an appropriate period of time. When a cell culture is used in the present invention, for example, at this stage, the cell culture may be taken out from the cultivation bottle and separated from the medium.

In the present invention, when the fruit body of Pleurotus erysinus is used in the present invention, the cultured cell culture after ripening is kept in a cultivation bottle in the same manner as in normal mushroom cultivation. After scraping the bacteria and then sprouting according to the sprouting step, the cultivation bottle may be left in a growth room and grown until the final harvest, and the obtained fruiting bodies may be collected.

The fruiting body of Pleurotus eryngis is commercially available from Hokuto Sangyo Co., Ltd. under the trade name Miyamashimeji, and can be used in the present invention.

The thus obtained cultured cells and fruiting bodies of Pleurotus erysinus (hereinafter abbreviated as "Pleurotus erysinus") contain a substance having an action of preventing and improving photoaging. Therefore, it is possible to use a homogenate of Pleurotus eryngie or a finely crushed dried product of Pleurotus eryngie in the skin cosmetic composition of the present invention. By doing so, it is preferable to take out the substance having the above-mentioned action in the form of being contained in the extract, and mix it with the skin cosmetic of the present invention. In the present invention, the extract may be any of such homogenate, pulverized product and extract, or a concentrate or dried product thereof, or a mixture thereof.

[0015] The extraction treatment of Pleurotus eryngis can be carried out by a continuous method, a batch method, or the like, by a general method, by immersion or digestion for an arbitrary time. For example, after shredding the fruit body of Pleurotus eryngii, 160-170
Extraction can be performed by immersing for a period of time or by heating and refluxing for 4 to 6 hours together with the extraction solvent, and then removing the extraction residue from the extract by filtration or the like to obtain an extract. The obtained extract can be concentrated by reduced pressure or ultrafiltration to obtain a concentrate. Further, if necessary, the solvent may be completely removed and dried or lyophilized.

As the solvent used for the above extraction, a polar solvent can be preferably exemplified. Specific examples of the polar solvent include polar organic solvents such as alcohols such as methanol and ethanol, ethers such as ethyl ether, ketones such as acetone, and esters such as ethyl acetate, and water. . These polar solvents can be used alone or in combination of two or more.

[0017] The extract of Pleurotus erysinae obtained in this manner is highly safe and excellent in the effects of preventing and improving photoaging. Next, the skin cosmetic of the present invention containing the extract of Pleurotus erysinus will be described. (2) Skin Cosmetic of the Present Invention The skin cosmetic of the present invention is characterized by containing the extract of Pleurotus erysinii.

The content of the extract of Pleurotus erysinus in the skin cosmetic of the present invention is preferably 0.001 to 10% by weight, more preferably 0.05 to 5% by dry solid content based on the total amount of the cosmetic. % By weight. When the content of the above Pleurotus erysinus extract as a dry solid content in the skin cosmetic of the present invention is less than 0.001% by weight, the effect of preventing and improving the photoaging symptoms of the skin may not be sufficiently obtained. Yes, and even if the amount exceeds 10% by weight, the effect corresponding to the increase may not be expected.

The dosage form of the skin cosmetic of the present invention is not particularly limited, and specific examples include creams, emulsions, oils, lotions, ointments, packs, aqueous gels, oil gels, bath preparations, and the like. However, in consideration of transdermal absorbability, creams, emulsions, oils and the like are more preferable dosage forms. These skin cosmetics can be produced by the same method as ordinary skin cosmetics, except that the extract of Pleurotus erysinus is appropriately selected and blended according to the dosage form.

In addition to the extract of Pleurotus eryngii, the skin cosmetic of the present invention contains components usually applied to skin cosmetics, such as water, hydrocarbons, esters, oils and fats, Alcohols, higher fatty acids, surfactants, polyhydric alcohols, lower alcohols, preservatives, ultraviolet absorbers, antioxidants, thickeners, humectants, powder ingredients, anti-inflammatory agents, pH adjusters, metals Blocking agents, saccharides, fragrances, coloring agents, various medicinal ingredients and the like are appropriately selected and blended as needed.

Specifically, liquid paraffin, petrolatum, squalane and the like as hydrocarbons, isopropyl myristate (IPM), synthetic gay wax, jojoba oil, carnauba wax and the like as esters, olive oil, tallow and the like as fats and oils Cetanol as higher alcohols,
Stearyl alcohol, etc., stearic acid, oleic acid, etc. as higher fatty acids,

Examples of the surfactants include anionic surfactants such as sodium lauryl sulfate and alkyl sulfosuccinate, cationic surfactants such as quaternary alkylamine salts, polyoxyethylene cetyl ether, polyethylene glycol monostearate, monostearin Nonionic surfactants such as glycerin acid, polyoxyethylene sorbitan monolaurate, polyoxyethylene lauryl ether, polyoxyethylene polyoxypropylene glycol, fatty acid monoglyceride, polyoxyethylene hydrogenated castor oil, and amphoteric surfactants such as alkyl betaine To

Glycerin, propylene glycol, 1,3-butylene glycol and the like as polyhydric alcohols
Ethanol and propanol as lower alcohols, parabens and chlorhexidine gluconate as preservatives, paraaminobenzoic acid derivatives and benzophenone derivatives as ultraviolet absorbers, vitamin E and butylhydroxytoluene (BHT) as antioxidants, etc. Gum arabic, carboxyvinyl polymer as a thickener,
Xanthan gum, polyvinyl alcohol, etc., polyethylene glycol etc. as a humectant,

Titanium oxide, silica gel,
Talc, acrylic acid-methacrylic acid copolymer, etc .; dipotassium glycyrrhetinate, tranexamic acid and derivatives thereof as anti-inflammatory agents; citrate, acetate as pH adjusters; disodium edetate as sequestering agent , Trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, gluconic acid, etc.
As sugars, glucose, fructose, mannose, sucrose, trehalose, sulfated trehalose, etc.,
Examples of the medicinal component include medicinal components for various purposes, for example, hyaluronic acid, placenta extract, ginseng extract, vitamins, and sterol glycosides.

[0025]

Embodiments of the present invention will be described below. First, Pleuretus /
A production example of an extract of Erinji will be described. In addition,
The fruiting body of Pleurotus eryngii used in the production example was Miyamushi Megumi manufactured by Hokuto Sangyo Co., Ltd.

[0026]

[Preparation Example 1] Ethanol extract of Pleurotus erysinus 100 g of Pleurotus erysinus fruiting body was cut into small pieces, and 1000 ml of ethanol was added thereto, followed by boiling extraction for 4 hours. After cooling, insolubles were removed by filtration to obtain an extract. The obtained extract was concentrated and dried to obtain 5.1 g of an ethanol extract of Pleurotus ellingii.

[0027]

[Production Example 2] Acetone extract of Pleurotus erysinus 100 g of Pleurotus erysinus fruiting body was cut into small pieces, and 1000 ml of acetone was added thereto, followed by boiling extraction for 4 hours. After cooling, insolubles were removed by filtration to obtain an extract. The obtained extract was concentrated and dried to obtain 4.3 g of Pleurotus erysinus acetone extract.

[0028]

[Preparation Example 3] Methanol extract of Pleurotus eryngie 100 g of Pleurotus eryngie fruit body was cut into small pieces, and 1000 ml of methanol was added thereto, followed by boiling extraction for 4 hours. After cooling, insolubles were removed by filtration to obtain an extract. The obtained extract was concentrated and dried to obtain 5.05 g of a methanol extract of Pleurotus erysinus.

<Effects of Pleurotus erysinus Extract to Prevent Photoageing> In humans, wrinkling is observed as one of the skin photoageing symptoms, especially in the outer corners of the face.
Here, a UV-wrinkle model improvement test of a hairless mouse was performed to evaluate the effect of Pleurotus erysinae extract on preventing photoaging. In addition, the Pleurotus eryngis extract obtained in the above Production Examples 1 to 3 was used for the evaluation.

UVB (60 mj / cm ² ) was applied to the back skin of 10 hairless mice (5 weeks old, female) in each group.
Irradiation was performed once a day at a rate of 5 days a week. After 9 weeks, formation of wrinkles due to photoaging on the back skin was confirmed. Thereafter, each of the Pleurotus erysinus extracts obtained in Production Examples 1 to 3 above was contained at a concentration shown in Table 1 at a concentration of 60%.
100 μl of an aqueous ethanol solution is administered once a day for 5 times a week.
The hairless mice were applied to wrinkle-forming sites on a daily basis. Nine weeks after the start of application, the degree of improvement of wrinkles due to photoaging of the skin was visually evaluated according to the following criteria.

(Judgment criteria)-: No wrinkle improvement effect is recognized. ±: Slight wrinkle improvement effect is recognized. +: Wrinkle improvement effect is recognized.

For comparison, a solution prepared by dissolving retinoic acid at a concentration of 0.2% in a 60% aqueous ethanol solution, which has been conventionally used as a drug having an action to prevent photoaging, was used as a control. In the same manner as described above, the degree of improvement in wrinkles when the aqueous solution alone was applied to hairless mice after UVB irradiation was evaluated. Table 1 shows the results.

[0033]

[Table 1]

As is clear from the results, the Pleurotus eryngii extract obtained in each of the above Production Examples was almost the same as retinoic acid conventionally used in the UV wrinkle model improvement test of hairless mice. Furthermore, it was confirmed that the skin was excellent in the effect of improving skin wrinkles due to photoaging. In addition, no deterioration of the skin condition such as redness was observed in the hairless mice during the experiment, and it was found that the Pleurotus eryngis extract had no problem in safety.

Next, examples of the skin cosmetic of the present invention containing the Pleurotus erysinus extract obtained in each of the above Production Examples will be described. The amounts used below are all by weight.

[0036]

Example 1 Oil-in-water cream The component A shown in Table 2 was heated and mixed at 80 ° C. Also, A
Separately from the components, component B was heated and mixed at 80 ° C. Then
After the component A and the component B are added and emulsified by stirring, the mixture
After cooling, an oil-in-water cream containing the ethanol extract of Pleurotus erysinae obtained in Production Example 1 was obtained. Similarly, a cream (Comparative Example 1) was prepared in which the extract of Pleurotus erysinus was replaced with purified water.

[0037]

[Table 2]

[0038]

Example 2 Emulsion The components A and B shown in Table 3 were each dissolved at 70 ° C. with stirring. Subsequently, the component A was added to the component B to perform preliminary emulsification, and further, the mixture was uniformly emulsified by a homomixer.
After the emulsification, the mixture was cooled to 30 ° C. with stirring to obtain an emulsion containing the acetone extract of Pleurotus eryngis obtained in Production Example 2 above.

[0039]

[Table 3]

[0040]

Example 3 Lotion A component shown in Table 4 was mixed and dissolved at room temperature. On the other hand, the component B was also mixed and dissolved at room temperature. Then
The component B was added to the component A and solubilized to obtain a lotion containing the methanol extract of Pleurotus erysinae obtained in Production Example 3 above.

[0041]

[Table 4]

<Evaluation of Skin Cosmetic of the Present Invention> Using the oil-in-water cream obtained in Example 1 and Comparative Example 1, a practical use test was conducted to evaluate the skin photoaging of the skin cosmetic of the present invention. The effect of prevention and improvement was evaluated.

Twelve Japanese men and women with clear wrinkles and spots on the corners of the eyes were asked to participate in the experiment (half-face test) as panelists. Prior to the experiment, photographs were taken of the left and right corners of the eyes of all panelists. Then, the oil-in-water cream of Example 1 was applied to one of the left and right outer corners of the panel, and the oil-in-water cream of Comparative Example 1 was applied to the other outer corner twice a day for 12 weeks. I used it. Twelve weeks after the start of use, the state of the right and left corners of the eyes of all the panelists was photographed in the same manner as before the start of the experiment. In this way, a total of four photographs were obtained for one panelist before and after the left and right eyes and before and after the experiment.
Using this, evaluation was performed by the following method.

Without giving any information on the contents of the experiment to a third party other than the above-mentioned panelists, two photographs of the right outer corner of the eye of a certain panelist taken before and after the experiment were shown. One of them was selected as having less wrinkles. If the selected photo was a post-use photo, the cream used for the right corner of the eye by this panel was evaluated as having a wrinkle-reducing effect, and a pre-use photo was evaluated as a natural exacerbation. When no selection was possible, it was determined that there was no effect. After that, two photographs of the left outer corner of the panel taken before and after the experiment were compared in the same manner, and the cream used by the panel for the left outer corner was evaluated. Furthermore, the wrinkle improving effect of each cream was similarly evaluated using photographs of 11 other panelists. The results are summarized in Table 5.

[0045]

[Table 5]

As is evident from the results, the cream of Example 1 containing the extract of Pleurotus erysinii had a better evaluation of the effect of improving wrinkles than the cream of the comparative example containing no extract. Was. In addition,
During the period of use of each cream in the above experiments, no panelists complained of troubles such as redness and rough skin.

[0047]

EFFECT OF THE INVENTION The skin cosmetic composition of the present invention is excellent in the effect of preventing and improving photoaging symptoms such as wrinkles on the skin, and can be used safely without harm to the skin.

Continued on the front page (72) Inventor Yukiko Mishiro 560 Polarization, Kashio-cho, Totsuka-ku, Yokohama-shi, Kanagawa Prefecture Inside the Totsuka Laboratory, Inc. Industrial Co., Ltd.

Claims (3)

[Claims] [1] Pleurotu Erinzy (Pleurotu)
s eryngii), which is an extract of the skin. 2. The skin cosmetic according to claim 1, wherein the extract is extracted with a polar solvent. 3. The skin cosmetic according to claim 1, wherein the content of the extract is 0.001 to 10% by weight as a dry solid content based on the total amount of the cosmetic.