JP2007511238A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2007511238A5 JP2007511238A5 JP2006541422A JP2006541422A JP2007511238A5 JP 2007511238 A5 JP2007511238 A5 JP 2007511238A5 JP 2006541422 A JP2006541422 A JP 2006541422A JP 2006541422 A JP2006541422 A JP 2006541422A JP 2007511238 A5 JP2007511238 A5 JP 2007511238A5
- Authority
- JP
- Japan
- Prior art keywords
- cell
- nucleic acid
- recombinant polypeptide
- cells
- laci
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 31
- 150000007523 nucleic acids Chemical class 0.000 claims 22
- 108020004707 nucleic acids Proteins 0.000 claims 20
- 102000039446 nucleic acids Human genes 0.000 claims 20
- 229920001184 polypeptide Polymers 0.000 claims 20
- 102000004196 processed proteins & peptides Human genes 0.000 claims 20
- 108090000765 processed proteins & peptides Proteins 0.000 claims 20
- 101150109249 lacI gene Proteins 0.000 claims 9
- 239000002207 metabolite Substances 0.000 claims 6
- 108090000790 Enzymes Proteins 0.000 claims 4
- 102000004190 Enzymes Human genes 0.000 claims 4
- BMCQMVFGOVHVNG-TUFAYURCSA-N cortisol 17-butyrate Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)CO)(OC(=O)CCC)[C@@]1(C)C[C@@H]2O BMCQMVFGOVHVNG-TUFAYURCSA-N 0.000 claims 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
- 229910019142 PO4 Inorganic materials 0.000 claims 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 2
- 230000002759 chromosomal effect Effects 0.000 claims 2
- 239000010452 phosphate Substances 0.000 claims 2
- 101150108812 proC gene Proteins 0.000 claims 2
- 101150116440 pyrF gene Proteins 0.000 claims 2
- 230000001105 regulatory effect Effects 0.000 claims 2
- DWAKNKKXGALPNW-BYPYZUCNSA-N (S)-1-pyrroline-5-carboxylic acid Chemical compound OC(=O)[C@@H]1CCC=N1 DWAKNKKXGALPNW-BYPYZUCNSA-N 0.000 claims 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- 241000894006 Bacteria Species 0.000 claims 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims 1
- 108010054278 Lac Repressors Proteins 0.000 claims 1
- 108010036940 Levansucrase Proteins 0.000 claims 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims 1
- 241000589516 Pseudomonas Species 0.000 claims 1
- 239000003242 anti bacterial agent Substances 0.000 claims 1
- 229940088710 antibiotic agent Drugs 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 230000005764 inhibitory process Effects 0.000 claims 1
- 238000003780 insertion Methods 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 230000004083 survival effect Effects 0.000 claims 1
- 229940035893 uracil Drugs 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US52342003P | 2003-11-19 | 2003-11-19 | |
| US60/523,420 | 2003-11-19 | ||
| US53714704P | 2004-01-16 | 2004-01-16 | |
| US60/537,147 | 2004-01-16 | ||
| PCT/US2004/038884 WO2005052151A1 (en) | 2003-11-19 | 2004-11-19 | Improved protein expression systems |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011275338A Division JP2012100669A (ja) | 2003-11-19 | 2011-12-16 | 改良タンパク質発現系 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2007511238A JP2007511238A (ja) | 2007-05-10 |
| JP2007511238A5 true JP2007511238A5 (enExample) | 2008-01-10 |
| JP5087741B2 JP5087741B2 (ja) | 2012-12-05 |
Family
ID=34636466
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006541422A Expired - Lifetime JP5087741B2 (ja) | 2003-11-19 | 2004-11-19 | 改良タンパク質発現系 |
| JP2011275338A Pending JP2012100669A (ja) | 2003-11-19 | 2011-12-16 | 改良タンパク質発現系 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011275338A Pending JP2012100669A (ja) | 2003-11-19 | 2011-12-16 | 改良タンパク質発現系 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US20050186666A1 (enExample) |
| EP (1) | EP1692282B1 (enExample) |
| JP (2) | JP5087741B2 (enExample) |
| KR (1) | KR101237651B1 (enExample) |
| CN (1) | CN1906294B (enExample) |
| AU (1) | AU2004293810B2 (enExample) |
| BR (1) | BRPI0416744A (enExample) |
| CA (1) | CA2545610C (enExample) |
| MX (1) | MXPA06005634A (enExample) |
| WO (1) | WO2005052151A1 (enExample) |
Families Citing this family (60)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9453251B2 (en) | 2002-10-08 | 2016-09-27 | Pfenex Inc. | Expression of mammalian proteins in Pseudomonas fluorescens |
| JP5087741B2 (ja) * | 2003-11-19 | 2012-12-05 | フェネックス インコーポレイテッド | 改良タンパク質発現系 |
| JP2008507294A (ja) | 2004-07-26 | 2008-03-13 | ダウ グローバル テクノロジーズ インコーポレイティド | 菌株遺伝子操作による改善されたタンパク質発現のための方法 |
| KR101461408B1 (ko) * | 2005-10-06 | 2014-11-13 | 어드밴스드 엑셀러레이터 어플리케이션즈 에스.에이. | 신규 선택 시스템 |
| WO2007055789A2 (en) * | 2005-10-31 | 2007-05-18 | Neose Technologies, Inc. | Expression of soluble therapeutic proteins |
| GB0602173D0 (en) | 2006-02-03 | 2006-03-15 | Avecia Ltd | Expression system |
| US8198045B2 (en) | 2006-04-19 | 2012-06-12 | Biogenerix Ag | Expression of O-glycosylated therapeutic proteins in prokaryotic microorganisms |
| WO2007142954A2 (en) * | 2006-05-30 | 2007-12-13 | Dow Global Technologies Inc. | Codon optimization method |
| KR100824541B1 (ko) * | 2007-02-26 | 2008-04-24 | 고려대학교 산학협력단 | 항산화 효소의 과발현을 통해 환경오염물질 분해능이향상된 균주 |
| EP2615172A1 (en) | 2007-04-27 | 2013-07-17 | Pfenex Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
| US9580719B2 (en) | 2007-04-27 | 2017-02-28 | Pfenex, Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
| US20100267067A1 (en) * | 2007-11-29 | 2010-10-21 | Novozymes A/S | Synthase Inhibitor Screening Method |
| JP2011505147A (ja) * | 2007-11-30 | 2011-02-24 | スカラブ ゲノミクス， エルエルシー | ｌａｃ発現システム |
| WO2010008764A1 (en) * | 2008-06-23 | 2010-01-21 | Dow Global Technologies Inc. | Pseudomonas fluorescens strains for production of extracellular recombinant protein |
| JP2010017131A (ja) * | 2008-07-10 | 2010-01-28 | Mitsui Eng & Shipbuild Co Ltd | モーレラ属細菌及びプライマー |
| US8153391B2 (en) | 2008-08-29 | 2012-04-10 | Bunge Oils, Inc. | Hydrolases, nucleic acids encoding them and methods for making and using them |
| US8357503B2 (en) | 2008-08-29 | 2013-01-22 | Bunge Oils, Inc. | Hydrolases, nucleic acids encoding them and methods for making and using them |
| US8198062B2 (en) | 2008-08-29 | 2012-06-12 | Dsm Ip Assets B.V. | Hydrolases, nucleic acids encoding them and methods for making and using them |
| US8623652B2 (en) * | 2009-04-06 | 2014-01-07 | Lucigen Corporation | Host-vector system for cloning and expressing genes |
| US9243253B2 (en) | 2009-05-11 | 2016-01-26 | Pfenex, Inc. | Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein |
| UA109884C2 (uk) | 2009-10-16 | 2015-10-26 | Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування | |
| UA111708C2 (uk) | 2009-10-16 | 2016-06-10 | Бандж Ойлз, Інк. | Спосіб рафінування олії |
| PE20130557A1 (es) | 2010-03-04 | 2013-05-19 | Pfenex Inc | Metodo para producir proteinas de interferon recombinantes solubles sin desnaturalizacion |
| BR112012024898A2 (pt) | 2010-03-30 | 2015-10-06 | Pfenex Inc | expressão de nível elevado de proteínas recombinantes de toxinas |
| EP2552949B1 (en) | 2010-04-01 | 2016-08-17 | Pfenex Inc. | Methods for g-csf production in a pseudomonas host cell |
| EP4242320A3 (en) | 2010-07-12 | 2023-11-29 | Inbiose N.V. | Metabolically engineered organisms for the production of added value bio-products |
| DK3366697T3 (da) | 2010-08-10 | 2021-03-29 | Sachdev Sidhu | Specifikke aktive stedshæmmere af enzymer eller substratbindingspartnere og fremgangsmåde til fremstilling heraf |
| PL222067B1 (pl) * | 2011-08-11 | 2016-06-30 | Inst Biotechnologii I Antybiotyków | Kaseta ekspresyjna, zastosowanie kasety ekspresyjnej, wektor ekspresyjny, komórka prokariotycznego gospodarza zawierająca wektor ekspresyjny, szczep bakteryjny oraz sposób wytwarzania polipeptydu |
| CN104302771A (zh) | 2012-03-30 | 2015-01-21 | 维莱尼姆公司 | 编码纤维素酶的基因 |
| US9169304B2 (en) | 2012-05-01 | 2015-10-27 | Pfenex Inc. | Process for purifying recombinant Plasmodium falciparum circumsporozoite protein |
| US9452205B2 (en) * | 2012-09-24 | 2016-09-27 | Montana State University | Recombinant Lactococcus lactis expressing Escherichia coli colonization factor antigen I (CFA/I) fimbriae and their methods of use |
| JP6448909B2 (ja) * | 2013-03-01 | 2019-01-09 | 学校法人関西学院 | 目的遺伝子を欠損した酢酸菌の製造方法及びその利用 |
| GB201308853D0 (en) | 2013-03-12 | 2013-07-03 | Verenium Corp | Genes encoding xylanase |
| GB201308828D0 (en) | 2013-03-12 | 2013-07-03 | Verenium Corp | Phytase |
| GB201308843D0 (en) | 2013-03-14 | 2013-07-03 | Verenium Corp | Phytase formulation |
| US10093909B2 (en) | 2013-07-25 | 2018-10-09 | Basf Enzymes Llc | Phytase |
| CA2951152A1 (en) | 2014-06-06 | 2015-12-10 | The Hospital For Sick Children | Soluble bacterial and fungal proteins and methods and uses thereof in inhibiting and dispersing biofilm |
| CN104004760B (zh) * | 2014-06-12 | 2017-11-28 | 华东理工大学 | 一种用于在米曲霉细胞分泌表达外源蛋白的表达设备及其米曲霉基因工程菌 |
| WO2015196275A1 (en) | 2014-06-27 | 2015-12-30 | National Research Council Of Canada (Nrc) | Cannabichromenic acid synthase from cannabis sativa |
| WO2016004024A1 (en) | 2014-07-02 | 2016-01-07 | Sutro Biopharma, Inc. | High growth capacity auxotrophic escherichia coli and methods of use |
| CN113444183B (zh) | 2014-12-01 | 2025-06-17 | 佩利肯科技控股公司 | 用于肽生产的融合伴侣 |
| RS65152B1 (sr) | 2015-02-05 | 2024-02-29 | Canem Holdings Llc | Preparati za lečenje granulomatoze sa poliangiitisom |
| KR20170068304A (ko) * | 2015-12-09 | 2017-06-19 | 삼성전자주식회사 | 대장균 및 코마가타에이박터 속 세포에서 복제가능한 벡터, 그를 포함한 세포, 및 그를 이용하는 방법 |
| WO2019063613A1 (en) | 2017-09-26 | 2019-04-04 | Dsm Ip Assets B.V. | ENZYMATIC ELIMINATION OF CHLOROPHYLL SUBSTRATES FROM TRIACYLGLYCEROL OILS |
| AU2018354069A1 (en) | 2017-10-27 | 2020-06-11 | Pelican Technology Holdings, Inc. | Bacterial leader sequences for periplasmic protein expression |
| WO2019083794A1 (en) | 2017-10-27 | 2019-05-02 | Pfenex Inc. | PROCESS FOR PRODUCING EAR ASPARAGINASE. RECOMBINED COLI |
| AU2018354067B2 (en) | 2017-10-27 | 2023-03-30 | Pfenex Inc. | Method for production of recombinant Erwinia asparaginase |
| CN108504677B (zh) * | 2018-03-06 | 2020-05-12 | 上海晶诺生物科技有限公司 | 一个用于构建谷氨酸基因家族缺失结核分枝杆菌的重组tm4噬菌体文库及其应用 |
| EP3946720A1 (en) | 2019-03-27 | 2022-02-09 | Bunge Global Innovation, LLC. | Silica adsorbent treatment for removal of chlorophyll derivatives from triacylglycerol-based oils |
| WO2020243642A2 (en) * | 2019-05-31 | 2020-12-03 | The Trustees Of Columbia University In The City Of New York | Multiply auxotrophic cell line for the production of recombinant proteins and methods thereof |
| EP4022070A1 (en) * | 2019-08-26 | 2022-07-06 | Voyager Therapeutics, Inc. | Controlled expression of viral proteins |
| CN111041029B (zh) * | 2019-12-23 | 2022-05-10 | 中国科学院天津工业生物技术研究所 | 一种强启动子及其在产维生素b12菌株中的应用 |
| CN113025601B (zh) * | 2019-12-25 | 2024-06-21 | 上海奥博生物医药股份有限公司 | 腈水解酶启动子优化表达及应用 |
| US11746352B2 (en) | 2019-12-30 | 2023-09-05 | Eligo Bioscience | Microbiome modulation of a host by delivery of DNA payloads with minimized spread |
| US12098372B2 (en) | 2019-12-30 | 2024-09-24 | Eligo Bioscience | Microbiome modulation of a host by delivery of DNA payloads with minimized spread |
| CN112080451A (zh) * | 2020-07-13 | 2020-12-15 | 宁波大学 | 一种嗜酸乳杆菌的食品级基因表达体系及其制备方法和应用 |
| WO2022144382A1 (en) | 2020-12-30 | 2022-07-07 | Eligo Bioscience | Chimeric receptor binding proteins resistant to proteolytic degradation |
| WO2022211829A1 (en) | 2021-03-30 | 2022-10-06 | Jazz Pharmaceuticals Ireland Ltd. | Dosing of recombinant l-asparaginase |
| CA3219207A1 (en) | 2021-05-12 | 2022-11-17 | Eligo Bioscience | Production bacterial cells and use thereof in production methods |
| WO2024211833A2 (en) | 2023-04-05 | 2024-10-10 | Primrose Bio, Inc. | Methods and compositions for nucleic acid synthesis |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4511503A (en) | 1982-12-22 | 1985-04-16 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
| US4755465A (en) | 1983-04-25 | 1988-07-05 | Genentech, Inc. | Secretion of correctly processed human growth hormone in E. coli and Pseudomonas |
| US4680264A (en) | 1983-07-01 | 1987-07-14 | Lubrizol Genetics, Inc. | Class II mobilizable gram-negative plasmid |
| US5281532A (en) | 1983-07-27 | 1994-01-25 | Mycogen Corporation | Pseudomas hosts transformed with bacillus endotoxin genes |
| DK594084A (da) * | 1984-12-12 | 1986-06-13 | Novo Industri As | Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning |
| US4695455A (en) | 1985-01-22 | 1987-09-22 | Mycogen Corporation | Cellular encapsulation of pesticides produced by expression of heterologous genes |
| US4695462A (en) | 1985-06-28 | 1987-09-22 | Mycogen Corporation | Cellular encapsulation of biological pesticides |
| GB8517071D0 (en) | 1985-07-05 | 1985-08-14 | Hoffmann La Roche | Gram-positive expression control sequence |
| US5232840A (en) * | 1986-03-27 | 1993-08-03 | Monsanto Company | Enhanced protein production in bacteria by employing a novel ribosome binding site |
| US5128130A (en) | 1988-01-22 | 1992-07-07 | Mycogen Corporation | Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens |
| US5055294A (en) | 1988-03-03 | 1991-10-08 | Mycogen Corporation | Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens |
| US5169760A (en) * | 1989-07-27 | 1992-12-08 | Mycogen Corporation | Method, vectors, and host cells for the control of expression of heterologous genes from lac operated promoters |
| JP2600090B2 (ja) | 1991-04-19 | 1997-04-16 | 農林水産省農業環境技術研究所長 | 宿主ベクター系 |
| US6566121B1 (en) * | 1991-06-13 | 2003-05-20 | Albert Einstein College Of Medicine Of Yeshiva University | Insertional mutations in mycobacteria |
| US5691185A (en) * | 1993-10-08 | 1997-11-25 | Chr. Hansen A/S | Lactic acid bacterial suppressor mutants and their use as selective markers and as means of containment in lactic acid bacteria |
| US5527883A (en) | 1994-05-06 | 1996-06-18 | Mycogen Corporation | Delta-endotoxin expression in pseudomonas fluorescens |
| AU6489996A (en) | 1995-07-14 | 1997-02-18 | Somatogen, Inc. | Methods for increasing protein expression |
| US7390645B2 (en) * | 1997-11-20 | 2008-06-24 | William Marsh Rice University | Lactose repressor proteins with increased operator DNA binding affinity |
| AU1467399A (en) | 1997-11-20 | 1999-06-15 | William Marsh Rice University | Lactose repressor proteins with altered ligand responsivity |
| DE19801120A1 (de) * | 1998-01-15 | 1999-07-22 | Basf Ag | Orotidin-5'-Phosphatdecarboxylase-Gen, Genkonstrukt enthaltend dieses Gen und seine Verwendung |
| JP4320689B2 (ja) | 1998-06-16 | 2009-08-26 | 株式会社力王 | 履物用先芯 |
| EP0972838B1 (en) * | 1998-07-15 | 2004-09-15 | Roche Diagnostics GmbH | Escherichia coli host/vector system based on antibiotic-free selection by complementation of an auxotrophy |
| US6291245B1 (en) * | 1998-07-15 | 2001-09-18 | Roche Diagnostics Gmbh | Host-vector system |
| US6413768B1 (en) * | 1998-12-02 | 2002-07-02 | University Of Maryland | Expression plasmids |
| US20040171824A1 (en) * | 2001-03-05 | 2004-09-02 | Scrable Heidi Jean | A Lac operator-repressor system |
| WO2003062399A2 (en) * | 2002-01-23 | 2003-07-31 | Diversa Corporation | Biocatalytic reduction of nitro groups |
| AU2002306484A1 (en) * | 2002-02-13 | 2003-09-04 | Dow Global Technologies Inc. | Over-expression of extremozyme genes in pseudomonads and closely related bacteria |
| DE10214406A1 (de) * | 2002-03-30 | 2003-10-09 | Nutrinova Gmbh | Verfahren und Marker zur einfachen Transformation und Selektion von rekombinanten Protisten |
| WO2003089455A2 (en) * | 2002-04-22 | 2003-10-30 | Dow Global Technologies Inc. | Low-cost production of peptides |
| JP5087741B2 (ja) | 2003-11-19 | 2012-12-05 | フェネックス インコーポレイテッド | 改良タンパク質発現系 |
-
2004
- 2004-11-19 JP JP2006541422A patent/JP5087741B2/ja not_active Expired - Lifetime
- 2004-11-19 CA CA2545610A patent/CA2545610C/en not_active Expired - Lifetime
- 2004-11-19 US US10/994,138 patent/US20050186666A1/en not_active Abandoned
- 2004-11-19 CN CN2004800407027A patent/CN1906294B/zh not_active Expired - Lifetime
- 2004-11-19 MX MXPA06005634A patent/MXPA06005634A/es active IP Right Grant
- 2004-11-19 BR BRPI0416744-9A patent/BRPI0416744A/pt not_active IP Right Cessation
- 2004-11-19 WO PCT/US2004/038884 patent/WO2005052151A1/en not_active Ceased
- 2004-11-19 KR KR1020067009838A patent/KR101237651B1/ko not_active Expired - Lifetime
- 2004-11-19 EP EP04811581A patent/EP1692282B1/en not_active Expired - Lifetime
- 2004-11-19 AU AU2004293810A patent/AU2004293810B2/en not_active Expired
-
2009
- 2009-07-30 US US12/512,930 patent/US8288127B2/en active Active
-
2011
- 2011-12-16 JP JP2011275338A patent/JP2012100669A/ja active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2007511238A5 (enExample) | ||
| ES2273356T3 (es) | Procedimiento para producir l-lisina. | |
| ES2373863T3 (es) | Procedimiento de producción de l-lisina. | |
| CA2442679A1 (en) | Methods for secretory production of proteins | |
| JP2023540797A (ja) | 塩基編集酵素 | |
| WO2003066818A3 (en) | Secretion, transcription and sporulation genes in bacillus clausii | |
| WO2005098016A3 (en) | Methods for producing hyaluronic acid | |
| CN107787361A (zh) | 生成l‑天冬氨酸衍生物的突变微生物及使用该突变微生物生成l‑天冬氨酸衍生物的方法 | |
| JP2009536017A5 (enExample) | ||
| CN115135759B (zh) | 新型葡萄糖胺-6-磷酸脱氨酶变体及使用其生产l-谷氨酸的方法 | |
| CN107287144B (zh) | 一种代谢改造的枯草芽孢杆菌生物转化细胞及其制备方法与应用 | |
| JP2025518813A (ja) | Ｌ－オルニチン生産能を有する微生物及びそれを用いたｌ－オルニチン生産方法 | |
| CN115029289B (zh) | 高产l-苏氨酸的基因工程菌及其构建方法与应用 | |
| CN105238820A (zh) | 一种用于大肠杆菌基因敲入的负筛选标记 | |
| CN106947776A (zh) | 多效调控蛋白CcpA及其在提升菌株发酵性能中的用途 | |
| JP2009171886A (ja) | 新規枯草菌変異株及びタンパク質の製造方法 | |
| KR20160111947A (ko) | 재조합 미생물의 생산 방법 | |
| An et al. | Reconstitution of TCA cycle involving L-isoleucine dioxygenase for hydroxylation of L-isoleucine in Escherichia coli using CRISPR-Cas9 | |
| US8927254B2 (en) | Pyrococcus furiosus strains and methods of using same | |
| TWI819552B (zh) | 用於生產高濃度左旋麩胺酸的菌株及使用其生產左旋麩胺酸的方法 | |
| CN115485373B (zh) | 新二氢硫辛酰胺乙酰基转移酶变体及使用其生产l-缬氨酸的方法 | |
| CN114981289B (zh) | 新蛋白变体及使用其生产l-赖氨酸的方法 | |
| CN105723219A (zh) | 重组微生物和其使用方法 | |
| CN103146739B (zh) | 双歧杆菌功能基因无痕敲除方法的建立 | |
| CN101137743B (zh) | 能够将XMP转化成GMP并保持与GMP降解有关的基因为失活状态的Escherichia菌株及其使用方法 |