JP2007505134A5 - - Google Patents
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- Publication number
- JP2007505134A5 JP2007505134A5 JP2006526315A JP2006526315A JP2007505134A5 JP 2007505134 A5 JP2007505134 A5 JP 2007505134A5 JP 2006526315 A JP2006526315 A JP 2006526315A JP 2006526315 A JP2006526315 A JP 2006526315A JP 2007505134 A5 JP2007505134 A5 JP 2007505134A5
- Authority
- JP
- Japan
- Prior art keywords
- solid support
- histidine
- polypeptide
- tagged polypeptide
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims 32
- 229920001184 polypeptide Polymers 0.000 claims 26
- 102000004196 processed proteins & peptides Human genes 0.000 claims 26
- 108090000765 processed proteins & peptides Proteins 0.000 claims 26
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 claims 24
- 239000007787 solid Substances 0.000 claims 18
- 239000000523 sample Substances 0.000 claims 9
- 239000012148 binding buffer Substances 0.000 claims 7
- 102000008015 Hemeproteins Human genes 0.000 claims 5
- 108010089792 Hemeproteins Proteins 0.000 claims 5
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims 5
- 239000012149 elution buffer Substances 0.000 claims 5
- 229910052725 zinc Inorganic materials 0.000 claims 5
- 239000011701 zinc Substances 0.000 claims 5
- 102000004169 proteins and genes Human genes 0.000 claims 4
- 108090000623 proteins and genes Proteins 0.000 claims 4
- 241000283973 Oryctolagus cuniculus Species 0.000 claims 3
- 229910017052 cobalt Inorganic materials 0.000 claims 3
- 239000010941 cobalt Substances 0.000 claims 3
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims 3
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims 3
- 230000003993 interaction Effects 0.000 claims 3
- 239000006166 lysate Substances 0.000 claims 3
- 210000001995 reticulocyte Anatomy 0.000 claims 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 238000010828 elution Methods 0.000 claims 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 1
- 102000001554 Hemoglobins Human genes 0.000 claims 1
- 108010054147 Hemoglobins Proteins 0.000 claims 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 1
- 239000012723 sample buffer Substances 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US50254403P | 2003-09-12 | 2003-09-12 | |
| US10/840,408 US7115397B2 (en) | 2003-09-12 | 2004-05-06 | Methods and kits for purifying his-tagged proteins |
| PCT/US2004/029580 WO2005035092A1 (en) | 2003-09-12 | 2004-09-10 | Methods and kits for purifying his-tagged proteins |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2007505134A JP2007505134A (ja) | 2007-03-08 |
| JP2007505134A5 true JP2007505134A5 (enExample) | 2007-09-13 |
Family
ID=34278838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006526315A Pending JP2007505134A (ja) | 2003-09-12 | 2004-09-10 | ヒスチジンタグ付加タンパク質を精製するための方法およびキット |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US7115397B2 (enExample) |
| EP (1) | EP1660206B1 (enExample) |
| JP (1) | JP2007505134A (enExample) |
| AT (1) | ATE403480T1 (enExample) |
| AU (1) | AU2004279361A1 (enExample) |
| CA (1) | CA2536066A1 (enExample) |
| DE (1) | DE602004015618D1 (enExample) |
| WO (1) | WO2005035092A1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2005203321A1 (en) * | 2004-08-03 | 2006-02-23 | Axis-Shield Diagnostics Limited | Assay |
| US20070092943A1 (en) * | 2005-09-28 | 2007-04-26 | The University Of Chicago | Versatile vectors for expression of foreign proteins in photosynthetic bacteria |
| JP5656339B2 (ja) * | 2007-03-28 | 2015-01-21 | Jsr株式会社 | タンパク質固定化担体およびその製造方法 |
| US8815533B2 (en) | 2010-01-30 | 2014-08-26 | University Of Rochester | Method and system for purifying and quantitating proteins using heme fusion tags |
| US8796419B2 (en) | 2010-05-19 | 2014-08-05 | Hoffmann-La Roche Inc. | Hydrophobic interaction chromatography method |
| CN109569546B (zh) * | 2018-07-05 | 2021-05-18 | 华侨大学 | 一种用于组氨酸标签蛋白纯化的螯合金属磁性纳米颗粒的制备方法及应用 |
| US12392795B2 (en) | 2020-03-09 | 2025-08-19 | Idexx Laboratories, Inc. | Matrix and associated sample or mixing cup used for removing components of a liquid sample |
| US12366506B2 (en) | 2020-03-09 | 2025-07-22 | Idexx Laboratories, Inc. | Method for removing interfering components of a liquid sample prior to dispensing same on a chemical reagent test slide |
| CN119178879A (zh) * | 2023-06-21 | 2024-12-24 | 广东菲鹏生物有限公司 | 降低背景信号的方法 |
| WO2025028658A1 (ja) * | 2023-08-03 | 2025-02-06 | 国立大学法人東京農工大学 | 免疫増強剤及び免疫増強方法並びにタンパク質の凝集体及び／又は会合体の製造方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5840851A (en) | 1993-07-23 | 1998-11-24 | Plomer; J. Jeffrey | Purification of hemoglobin |
| US5962641A (en) | 1996-08-16 | 1999-10-05 | Clontech Laboratories, Inc. | Method for purification of recombinant proteins |
| WO1999057992A1 (en) | 1998-05-14 | 1999-11-18 | Clontech Laboratories, Inc. | Compositions and methods for protein purification based on a metal ion affinity site |
| US6232083B1 (en) * | 1999-03-12 | 2001-05-15 | The Research Foundation Of State University Of New York | Methods and compositions for screening cloned proteins |
| ATE320443T1 (de) * | 1999-07-15 | 2006-04-15 | Qiagen Gmbh | Verfahren zur trennung von teilchenförmigen substraten aus einer lösung indem man den teilchenverlust minimiert |
| WO2002037100A2 (en) | 2000-10-30 | 2002-05-10 | Lytton Simon D | Novel applications of nickel nitrilotriacetic acid (ni-nta) resin: hemeprotein removal, recovery, and purification from biological samples |
| CA2440530A1 (en) * | 2002-09-11 | 2004-03-11 | Henkel Kommanditgesellschaft Auf Aktien | Coating removal compositions |
| EP1581337A4 (en) | 2002-10-18 | 2008-05-07 | Promega Corp | COMPOSITION FOR DISCONNECTING MOLECULES |
-
2004
- 2004-05-06 US US10/840,408 patent/US7115397B2/en not_active Expired - Fee Related
- 2004-09-10 WO PCT/US2004/029580 patent/WO2005035092A1/en not_active Ceased
- 2004-09-10 CA CA002536066A patent/CA2536066A1/en not_active Abandoned
- 2004-09-10 EP EP04788679A patent/EP1660206B1/en not_active Expired - Lifetime
- 2004-09-10 JP JP2006526315A patent/JP2007505134A/ja active Pending
- 2004-09-10 AT AT04788679T patent/ATE403480T1/de not_active IP Right Cessation
- 2004-09-10 AU AU2004279361A patent/AU2004279361A1/en not_active Abandoned
- 2004-09-10 DE DE602004015618T patent/DE602004015618D1/de not_active Expired - Fee Related
-
2005
- 2005-01-10 US US11/032,281 patent/US7150973B2/en not_active Expired - Fee Related
- 2005-01-10 US US11/032,282 patent/US20050209129A1/en not_active Abandoned
-
2006
- 2006-08-22 US US11/466,274 patent/US7339036B2/en not_active Expired - Fee Related
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