DE602004015618D1 - Verfahren und ausrüstungen zur reinigung von proteinen mit his-tag - Google Patents

Verfahren und ausrüstungen zur reinigung von proteinen mit his-tag

Info

Publication number
DE602004015618D1
DE602004015618D1 DE602004015618T DE602004015618T DE602004015618D1 DE 602004015618 D1 DE602004015618 D1 DE 602004015618D1 DE 602004015618 T DE602004015618 T DE 602004015618T DE 602004015618 T DE602004015618 T DE 602004015618T DE 602004015618 D1 DE602004015618 D1 DE 602004015618D1
Authority
DE
Germany
Prior art keywords
tag
equipment
proteins
cleaning
cleaning proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE602004015618T
Other languages
German (de)
English (en)
Inventor
Tonny Johnson
Rebecca Godat
Laurie Engel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of DE602004015618D1 publication Critical patent/DE602004015618D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J45/00—Ion-exchange in which a complex or a chelate is formed; Use of material as complex or chelate forming ion-exchangers; Treatment of material for improving the complex or chelate forming ion-exchange properties
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Detergent Compositions (AREA)
DE602004015618T 2003-09-12 2004-09-10 Verfahren und ausrüstungen zur reinigung von proteinen mit his-tag Expired - Fee Related DE602004015618D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US50254403P 2003-09-12 2003-09-12
US10/840,408 US7115397B2 (en) 2003-09-12 2004-05-06 Methods and kits for purifying his-tagged proteins
PCT/US2004/029580 WO2005035092A1 (en) 2003-09-12 2004-09-10 Methods and kits for purifying his-tagged proteins

Publications (1)

Publication Number Publication Date
DE602004015618D1 true DE602004015618D1 (de) 2008-09-18

Family

ID=34278838

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004015618T Expired - Fee Related DE602004015618D1 (de) 2003-09-12 2004-09-10 Verfahren und ausrüstungen zur reinigung von proteinen mit his-tag

Country Status (8)

Country Link
US (4) US7115397B2 (enExample)
EP (1) EP1660206B1 (enExample)
JP (1) JP2007505134A (enExample)
AT (1) ATE403480T1 (enExample)
AU (1) AU2004279361A1 (enExample)
CA (1) CA2536066A1 (enExample)
DE (1) DE602004015618D1 (enExample)
WO (1) WO2005035092A1 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005203321A1 (en) * 2004-08-03 2006-02-23 Axis-Shield Diagnostics Limited Assay
US20070092943A1 (en) * 2005-09-28 2007-04-26 The University Of Chicago Versatile vectors for expression of foreign proteins in photosynthetic bacteria
JP5656339B2 (ja) * 2007-03-28 2015-01-21 Jsr株式会社 タンパク質固定化担体およびその製造方法
US8815533B2 (en) 2010-01-30 2014-08-26 University Of Rochester Method and system for purifying and quantitating proteins using heme fusion tags
US8796419B2 (en) 2010-05-19 2014-08-05 Hoffmann-La Roche Inc. Hydrophobic interaction chromatography method
CN109569546B (zh) * 2018-07-05 2021-05-18 华侨大学 一种用于组氨酸标签蛋白纯化的螯合金属磁性纳米颗粒的制备方法及应用
US12392795B2 (en) 2020-03-09 2025-08-19 Idexx Laboratories, Inc. Matrix and associated sample or mixing cup used for removing components of a liquid sample
US12366506B2 (en) 2020-03-09 2025-07-22 Idexx Laboratories, Inc. Method for removing interfering components of a liquid sample prior to dispensing same on a chemical reagent test slide
CN119178879A (zh) * 2023-06-21 2024-12-24 广东菲鹏生物有限公司 降低背景信号的方法
WO2025028658A1 (ja) * 2023-08-03 2025-02-06 国立大学法人東京農工大学 免疫増強剤及び免疫増強方法並びにタンパク質の凝集体及び／又は会合体の製造方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5840851A (en) 1993-07-23 1998-11-24 Plomer; J. Jeffrey Purification of hemoglobin
US5962641A (en) 1996-08-16 1999-10-05 Clontech Laboratories, Inc. Method for purification of recombinant proteins
WO1999057992A1 (en) 1998-05-14 1999-11-18 Clontech Laboratories, Inc. Compositions and methods for protein purification based on a metal ion affinity site
US6232083B1 (en) * 1999-03-12 2001-05-15 The Research Foundation Of State University Of New York Methods and compositions for screening cloned proteins
ATE320443T1 (de) * 1999-07-15 2006-04-15 Qiagen Gmbh Verfahren zur trennung von teilchenförmigen substraten aus einer lösung indem man den teilchenverlust minimiert
WO2002037100A2 (en) 2000-10-30 2002-05-10 Lytton Simon D Novel applications of nickel nitrilotriacetic acid (ni-nta) resin: hemeprotein removal, recovery, and purification from biological samples
CA2440530A1 (en) * 2002-09-11 2004-03-11 Henkel Kommanditgesellschaft Auf Aktien Coating removal compositions
EP1581337A4 (en) 2002-10-18 2008-05-07 Promega Corp COMPOSITION FOR DISCONNECTING MOLECULES

Also Published As

Publication number Publication date
US7115397B2 (en) 2006-10-03
US20050209129A1 (en) 2005-09-22
AU2004279361A1 (en) 2005-04-21
EP1660206B1 (en) 2008-08-06
US20050059809A1 (en) 2005-03-17
JP2007505134A (ja) 2007-03-08
EP1660206A1 (en) 2006-05-31
CA2536066A1 (en) 2005-04-21
ATE403480T1 (de) 2008-08-15
US20050209444A1 (en) 2005-09-22
US20060281906A1 (en) 2006-12-14
US7339036B2 (en) 2008-03-04
US7150973B2 (en) 2006-12-19
WO2005035092A1 (en) 2005-04-21

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee